Q9H0I9 (TKTL2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transketolase-like protein 2
EC=2.2.1.1

Gene names

Name:

TKTL2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	626 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an essential role in total transketolase activity and cell proliferation in cancer cells; after transfection with anti-TKTL1 siRNA, total transketolase activity dramatically decreases and proliferation was significantly inhibited in cancer cells. Play a pivotal role in carcinogenesis. Ref.5
Catalytic activity	Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate.
Cofactor	Binds 1 calcium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Tissue specificity	Overexpressed in hepatoma cancer cells. Ref.5
Sequence similarities	Belongs to the transketolase family.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	Calcium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay. Source: LIFEdb
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW transketolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 626	626	Transketolase-like protein 2		PRO_0000285200
Sites
	Metal binding	156	1	Calcium By similarity
	Metal binding	186	1	Calcium By similarity
Amino acid modifications
	Modified residue	325	1	Phosphotyrosine Ref.6
Natural variations
	Natural variant	442	1	R → Q. Corresponds to variant rs3811750 [ dbSNP | Ensembl ].		VAR_031990
	Natural variant	590	1	Q → H. Ref.4 Corresponds to variant rs11735477 [ dbSNP | Ensembl ].		VAR_031991
Experimental info
	Sequence conflict	54	1	F → L in BAB71427. Ref.2
	Sequence conflict	302	1	I → V in AAH28707. Ref.4
	Sequence conflict	311	1	P → H in AAH28707. Ref.4
	Sequence conflict	406	1	M → I in BAB71427. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9H0I9 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 446C4EC81EDC0EA4	FASTA	626	67,877
	10 20 30 40 50 60 MMANDAKPDV KTVQVLRDTA NRLRIHSIRA TCASGSGQLT SCCSAAEVVS VLFFHTMKYK 70 80 90 100 110 120 QTDPEHPDND RFILSRGHAA PILYAAWVEV GDISESDLLN LRKLHSDLER HPTPRLPFVD 130 140 150 160 170 180 VATGSLGQGL GTACGMAYTG KYLDKASYRV FCLMGDGESS EGSVWEAFAF ASHYNLDNLV 190 200 210 220 230 240 AVFDVNRLGQ SGPAPLEHGA DIYQNCCEAF GWNTYLVDGH DVEALCQAFW QASQVKNKPT 250 260 270 280 290 300 AIVAKTFKGR GIPNIEDAEN WHGKPVPKER ADAIVKLIES QIQTNENLIP KSPVEDSPQI 310 320 330 340 350 360 SITDIKMTSP PAYKVGDKIA TQKTYGLALA KLGRANERVI VLSGDTMNST FSEIFRKEHP 370 380 390 400 410 420 ERFIECIIAE QNMVSVALGC ATRGRTIAFA GAFAAFFTRA FDQLRMGAIS QANINLIGSH 430 440 450 460 470 480 CGVSTGEDGV SQMALEDLAM FRSIPNCTVF YPSDAISTEH AIYLAANTKG MCFIRTSQPE 490 500 510 520 530 540 TAVIYTPQEN FEIGQAKVVR HGVNDKVTVI GAGVTLHEAL EAADHLSQQG ISVRVIDPFT 550 560 570 580 590 600 IKPLDAATII SSAKATGGRV ITVEDHYREG GIGEAVCAAV SREPDILVHQ LAVSGVPQRG 610 620 KTSELLDMFG ISTRHIIAAV TLTLMK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT HIS-590. Tissue: Testis.
[5]	"Gene silencing of TKTL1 by RNAi inhibits cell proliferation in human hepatoma cells." Zhang S., Yang J.-H., Guo C.-K., Cai P.-C. Cancer Lett. 253:108-114(2007) [PubMed: 17321041] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-325, MASS SPECTROMETRY. Tissue: Lung carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL136779 mRNA. Translation: CAB66713.1. AK057325 mRNA. Translation: BAB71427.1. CR533560 mRNA. Translation: CAG38591.1. BC028707 mRNA. Translation: AAH28707.4. BC125101 mRNA. Translation: AAI25102.1. BC142943 mRNA. Translation: AAI42944.1.
IPI	IPI00549825.
RefSeq	NP_115512.3. NM_032136.4.
UniGene	Hs.303923.
3D structure databases
HSSP	HSSP built from PDB template 1W88 based on UniProtKB P21874.
ProteinModelPortal	Q9H0I9.
SMR	Q9H0I9. Positions 7-620.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H0I9. 1 interaction.
MINT	MINT-1451143.
STRING	Q9H0I9.
PTM databases
PhosphoSite	Q9H0I9.
Polymorphism databases
DMDM	74717985.
Proteomic databases
PRIDE	Q9H0I9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000280605; ENSP00000280605; ENSG00000151005.
GeneID	84076.
KEGG	hsa:84076.
NMPDR	fig|9606.3.peg.24819.
UCSC	uc003iqp.2. human.
Organism-specific databases
CTD	84076.
GeneCards	GC04M164392.
H-InvDB	HIX0004606.
HGNC	HGNC:25313. TKTL2.
HPA	HPA043797.
neXtProt	NX_Q9H0I9.
PharmGKB	PA142670806.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000005240.
HOGENOM	HBG465450.
HOVERGEN	HBG004036.
InParanoid	Q9H0I9.
OMA	DMFGIST.
OrthoDB	EOG4R23TG.
PhylomeDB	Q9H0I9.
Gene expression databases
ArrayExpress	Q9H0I9.
Bgee	Q9H0I9.
CleanEx	HS_TKTL2.
Genevestigator	Q9H0I9.
Family and domain databases
InterPro	IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K00615.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. PF00456. Transketolase_N. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. False negative. PS00802. TRANSKETOLASE_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	73287.

Entry information

Entry name

TKTL2_HUMAN

Accession

Primary (citable) accession number: Q9H0I9
Secondary accession number(s): A4FVB4, Q8NCT0, Q96M82

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.