Q9H0E3 (SP130_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone deacetylase complex subunit SAP130

Alternative name(s):
130 kDa Sin3-associated polypeptide
Sin3-associated polypeptide p130

Gene names

Name:

SAP130

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1048 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. Ref.1
Subunit structure	Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts (released by dead or dying cells) with CLEC4E By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Expressed in various cancer cell ines. Ref.1
Domain	The N-terminus may interact with a transcriptional coactivator. The C-terminus may interact with HDAC-dependent and HDAC-independent corepressors.
Post-translational modification	Acetylated Probable.
Sequence similarities	Belongs to the SAP130 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Repressor
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histone H3 acetylation Inferred from direct assay. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	STAGA complex Inferred from direct assay. Source: UniProtKB
Molecular function	transcription coactivator activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H0E3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H0E3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-470: Missing.
     678-678: D → DGMAVRKTLIPPQPPDVASPRVESSMRSTSGSPRPA

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1048	1048	Histone deacetylase complex subunit SAP130		PRO_0000283736
Regions
	Region	836 – 1047	212	Interactions with SIN3A and HDAC1
	Compositional bias	81 – 85	5	Poly-Ser
	Compositional bias	124 – 169	46	Pro-rich
	Compositional bias	234 – 238	5	Poly-Ala
	Compositional bias	709 – 836	128	Pro-rich
Amino acid modifications
	Modified residue	355	1	Phosphothreonine Ref.7
	Modified residue	442	1	Phosphoserine Ref.7
	Modified residue	454	1	Phosphoserine Ref.6
	Modified residue	455	1	Phosphoserine Ref.6
	Modified residue	875	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	1 – 470	470	Missing in isoform 2.		VSP_024355
	Alternative sequence	678	1	D → DGMAVRKTLIPPQPPDVASP RVESSMRSTSGSPRPA in isoform 2.		VSP_024356

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: EB4E74E35BA07AEE	FASTA	1,048	110,324
	10 20 30 40 50 60 MGPPRHPQAG EIEAGGAGGG RRLQVEMSSQ QFPRLGAPST GLSQAPSQIA NSGSAGLINP 70 80 90 100 110 120 AATVNDESGR DSEVSAREHM SSSSSLQSRE EKQEPVVVRP YPQVQMLSTH HAVASATPVA 130 140 150 160 170 180 VTAPPAHLTP AVPLSFSEGL MKPPPKPTMP SRPIAPAPPS TLSLPPKVPG QVTVTMESSI 190 200 210 220 230 240 PQASAIPVAT ISGQQGHPSN LHHIMTTNVQ MSIIRSNAPG PPLHIGASHL PRGAAAAAVM 250 260 270 280 290 300 SSSKVTTVLR PTSQLPNAAT AQPAVQHIIH QPIQSRPPVT TSNAIPPAVV ATVSATRAQS 310 320 330 340 350 360 PVITTTAAHA TDSALSRPTL SIQHPPSAAI SIQRPAQSRD VTTRITLPSH PALGTPKQQL 370 380 390 400 410 420 HTMAQKTIFS TGTPVAAATV APILATNTIP SATTAGSVSH TQAPTSTIVT MTVPSHSSHA 430 440 450 460 470 480 TAVTTSNIPV AKVVPQQITH TSPRIQPDYP AERSSLIPIS GHRASPNPVA METRSDNRPS 490 500 510 520 530 540 VPVQFQYFLP TYPPSAYPLA AHTYTPITSS VSTIRQYPVS AQAPNSAITA QTGVGVASTV 550 560 570 580 590 600 HLNPMQLMTV DASHARHIQG IQPAPISTQG IQPAPIGTPG IQPAPLGTQG IHSATPINTQ 610 620 630 640 650 660 GLQPAPMGTQ QPQPEGKTSA VVLADGATIV ANPISNPFSA APAATTVVQT HSQSASTNAP 670 680 690 700 710 720 AQGSSPRPSI LRKKPATDGA KPKSEIHVSM ATPVTVSMET VSNQNNDQPT IAVPPTAQQP 730 740 750 760 770 780 PPTIPTMIAA ASPPSQPAVA LSTIPGAVPI TPPITTIAAA PPPSVTVGGS LSSVLGPPVP 790 800 810 820 830 840 EIKVKEEVEP MDIMRPVSAV PPLATNTVSP SLALLANNLS MPTSDLPPGA SPRKKPRKQQ 850 860 870 880 890 900 HVISTEEGDM METNSTDDEK STAKSLLVKA EKRKSPPKEY IDEEGVRYVP VRPRPPITLL 910 920 930 940 950 960 RHYRNPWKAA YHHFQRYSDV RVKEEKKAML QEIANQKGVS CRAQGWKVHL CAAQLLQLTN 970 980 990 1000 1010 1020 LEHDVYERLT NLQEGIIPKK KAATDDDLHR INELIQGNMQ RCKLVMDQIS EARDSMLKVL 1030 1040 DHKDRVLKLL NKNGTVKKVS KLKRKEKV
	Isoform 2 [UniParc]. Checksum: 6392B91A2F6FCDF5	FASTA	613	65,689
	10 20 30 40 50 60 METRSDNRPS VPVQFQYFLP TYPPSAYPLA AHTYTPITSS VSTIRQYPVS AQAPNSAITA 70 80 90 100 110 120 QTGVGVASTV HLNPMQLMTV DASHARHIQG IQPAPISTQG IQPAPIGTPG IQPAPLGTQG 130 140 150 160 170 180 IHSATPINTQ GLQPAPMGTQ QPQPEGKTSA VVLADGATIV ANPISNPFSA APAATTVVQT 190 200 210 220 230 240 HSQSASTNAP AQGSSPRPSI LRKKPATDGM AVRKTLIPPQ PPDVASPRVE SSMRSTSGSP 250 260 270 280 290 300 RPAGAKPKSE IHVSMATPVT VSMETVSNQN NDQPTIAVPP TAQQPPPTIP TMIAAASPPS 310 320 330 340 350 360 QPAVALSTIP GAVPITPPIT TIAAAPPPSV TVGGSLSSVL GPPVPEIKVK EEVEPMDIMR 370 380 390 400 410 420 PVSAVPPLAT NTVSPSLALL ANNLSMPTSD LPPGASPRKK PRKQQHVIST EEGDMMETNS 430 440 450 460 470 480 TDDEKSTAKS LLVKAEKRKS PPKEYIDEEG VRYVPVRPRP PITLLRHYRN PWKAAYHHFQ 490 500 510 520 530 540 RYSDVRVKEE KKAMLQEIAN QKGVSCRAQG WKVHLCAAQL LQLTNLEHDV YERLTNLQEG 550 560 570 580 590 600 IIPKKKAATD DDLHRINELI QGNMQRCKLV MDQISEARDS MLKVLDHKDR VLKLLNKNGT 610 VKKVSKLKRK EKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of three new components of the mSin3A corepressor complex." Fleischer T.C., Yun U.J., Ayer D.E. Mol. Cell. Biol. 23:3456-3467(2003) [PubMed: 12724404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, ACETYLATION, IDENTIFICATION IN A MSIN3A COREPRESSOR COMPLEX WITH SIN3A; SAP130; SUDS3; ARID4B; HDAC1 AND HDAC2.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-454 AND SER-455, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-355; SER-442 AND SER-875, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY220791 mRNA. Translation: AAO63591.1. AL136833 mRNA. Translation: CAB66767.1. AK022823 mRNA. Translation: BAB14261.1. AC118060 Genomic DNA. Translation: AAX88975.1. AC012306 Genomic DNA. Translation: AAY14878.1. BC017453 mRNA. Translation: AAH17453.2. BC117255 mRNA. Translation: AAI17256.1.
IPI	IPI00002220. IPI00748953.
RefSeq	NP_001139400.1. NM_001145928.1. NP_078821.2. NM_024545.3.
UniGene	Hs.32995.
3D structure databases
ProteinModelPortal	Q9H0E3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H0E3. 2 interactions.
MINT	MINT-1185872.
STRING	Q9H0E3.
PTM databases
PhosphoSite	Q9H0E3.
Polymorphism databases
DMDM	74717977.
Proteomic databases
PRIDE	Q9H0E3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000259235; ENSP00000259235; ENSG00000136715.
GeneID	79595.
KEGG	hsa:79595.
UCSC	uc002tpn.1. human. uc002tpp.1. human.
Organism-specific databases
CTD	79595.
GeneCards	GC02M128792.
H-InvDB	HIX0020746.
HGNC	HGNC:29813. SAP130.
HPA	HPA034664.
MIM	609697. gene.
neXtProt	NX_Q9H0E3.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00440000037733.
HOVERGEN	HBG106600.
OrthoDB	EOG49W2GD.
PhylomeDB	Q9H0E3.
Gene expression databases
ArrayExpress	Q9H0E3.
Bgee	Q9H0E3.
CleanEx	HS_SAP130.
Genevestigator	Q9H0E3.
Family and domain databases
InterPro	IPR024137. His_deAcase_cplx_SAP130. [Graphical view]
PANTHER	PTHR13497. PTHR13497. 1 hit.
ProtoNet	Search...
Other
NextBio	68614.
SOURCE	Search...

Entry information

Entry name

SP130_HUMAN

Accession

Primary (citable) accession number: Q9H0E3
Secondary accession number(s): C9K0X9 , Q4ZFV4, Q53T46, Q8WVW4, Q9H9G8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.