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Protein

Integrin-linked kinase-associated serine/threonine phosphatase 2C

Gene

ILKAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Enzyme regulationi

Inhibited rather than stimulated by magnesium.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi152Manganese 1By similarity1
Metal bindingi152Manganese 2By similarity1
Metal bindingi153Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi326Manganese 2By similarity1
Metal bindingi381Manganese 2By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:HS05617-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin-linked kinase-associated serine/threonine phosphatase 2C (EC:3.1.3.16)
Short name:
ILKAP
Gene namesi
Name:ILKAP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:15566. ILKAP.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi152D → A: Loss of >90% of activity. 1 Publication1
Mutagenesisi154H → D: Loss of >90% of activity. 1 Publication1
Mutagenesisi154H → L: Loss of >90% of activity. 1 Publication1

Organism-specific databases

DisGeNETi80895.
OpenTargetsiENSG00000132323.
PharmGKBiPA29856.

Polymorphism and mutation databases

BioMutaiILKAP.
DMDMi74752560.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002722711 – 392Integrin-linked kinase-associated serine/threonine phosphatase 2CAdd BLAST392

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei13PhosphoserineCombined sources1
Modified residuei210N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H0C8.
MaxQBiQ9H0C8.
PaxDbiQ9H0C8.
PeptideAtlasiQ9H0C8.
PRIDEiQ9H0C8.

PTM databases

DEPODiQ9H0C8.
iPTMnetiQ9H0C8.
PhosphoSitePlusiQ9H0C8.

Expressioni

Tissue specificityi

Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.1 Publication

Gene expression databases

BgeeiENSG00000132323.
CleanExiHS_ILKAP.
ExpressionAtlasiQ9H0C8. baseline and differential.
GenevisibleiQ9H0C8. HS.

Organism-specific databases

HPAiHPA004752.
HPA056099.

Interactioni

Subunit structurei

Interacts with ILK. Specific association with ILK is independent of the catalytic activity of either partner.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ILKQ134183EBI-2620298,EBI-747644

Protein-protein interaction databases

BioGridi123343. 26 interactors.
IntActiQ9H0C8. 12 interactors.
STRINGi9606.ENSP00000254654.

Structurei

3D structure databases

ProteinModelPortaliQ9H0C8.
SMRiQ9H0C8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 390PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST283

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
GeneTreeiENSGT00840000129732.
HOGENOMiHOG000233896.
HOVERGENiHBG054286.
InParanoidiQ9H0C8.
KOiK17500.
OMAiPDIKRCQ.
OrthoDBiEOG091G0L5M.
PhylomeDBiQ9H0C8.
TreeFamiTF313513.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H0C8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLFGDLPEP ERSPRPAAGK EAQKGPLLFD DLPPASSTDS GSGGPLLFDD
60 70 80 90 100
LPPASSGDSG SLATSISQMV KTEGKGAKRK TSEEEKNGSE ELVEKKVCKA
110 120 130 140 150
SSVIFGLKGY VAERKGEREE MQDAHVILND ITEECRPPSS LITRVSYFAV
160 170 180 190 200
FDGHGGIRAS KFAAQNLHQN LIRKFPKGDV ISVEKTVKRC LLDTFKHTDE
210 220 230 240 250
EFLKQASSQK PAWKDGSTAT CVLAVDNILY IANLGDSRAI LCRYNEESQK
260 270 280 290 300
HAALSLSKEH NPTQYEERMR IQKAGGNVRD GRVLGVLEVS RSIGDGQYKR
310 320 330 340 350
CGVTSVPDIR RCQLTPNDRF ILLACDGLFK VFTPEEAVNF ILSCLEDEKI
360 370 380 390
QTREGKSAAD ARYEAACNRL ANKAVQRGSA DNVTVMVVRI GH
Length:392
Mass (Da):42,907
Last modified:March 1, 2001 - v1
Checksum:iAF6ACC98508CBEA3
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06154266I → M.1 PublicationCorresponds to variant rs34371548dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY024365 mRNA. Translation: AAK07736.1.
AL136850 mRNA. Translation: CAB66784.1.
CR533533 mRNA. Translation: CAG38564.1.
AK001043 mRNA. Translation: BAG50851.1.
AC016757 Genomic DNA. Translation: AAY24336.1.
BC006576 mRNA. Translation: AAH06576.1.
CCDSiCCDS2526.1.
RefSeqiNP_110395.1. NM_030768.2.
UniGeneiHs.92033.

Genome annotation databases

EnsembliENST00000254654; ENSP00000254654; ENSG00000132323.
GeneIDi80895.
KEGGihsa:80895.
UCSCiuc002vxv.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY024365 mRNA. Translation: AAK07736.1.
AL136850 mRNA. Translation: CAB66784.1.
CR533533 mRNA. Translation: CAG38564.1.
AK001043 mRNA. Translation: BAG50851.1.
AC016757 Genomic DNA. Translation: AAY24336.1.
BC006576 mRNA. Translation: AAH06576.1.
CCDSiCCDS2526.1.
RefSeqiNP_110395.1. NM_030768.2.
UniGeneiHs.92033.

3D structure databases

ProteinModelPortaliQ9H0C8.
SMRiQ9H0C8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123343. 26 interactors.
IntActiQ9H0C8. 12 interactors.
STRINGi9606.ENSP00000254654.

PTM databases

DEPODiQ9H0C8.
iPTMnetiQ9H0C8.
PhosphoSitePlusiQ9H0C8.

Polymorphism and mutation databases

BioMutaiILKAP.
DMDMi74752560.

Proteomic databases

EPDiQ9H0C8.
MaxQBiQ9H0C8.
PaxDbiQ9H0C8.
PeptideAtlasiQ9H0C8.
PRIDEiQ9H0C8.

Protocols and materials databases

DNASUi80895.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254654; ENSP00000254654; ENSG00000132323.
GeneIDi80895.
KEGGihsa:80895.
UCSCiuc002vxv.4. human.

Organism-specific databases

CTDi80895.
DisGeNETi80895.
GeneCardsiILKAP.
HGNCiHGNC:15566. ILKAP.
HPAiHPA004752.
HPA056099.
neXtProtiNX_Q9H0C8.
OpenTargetsiENSG00000132323.
PharmGKBiPA29856.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
GeneTreeiENSGT00840000129732.
HOGENOMiHOG000233896.
HOVERGENiHBG054286.
InParanoidiQ9H0C8.
KOiK17500.
OMAiPDIKRCQ.
OrthoDBiEOG091G0L5M.
PhylomeDBiQ9H0C8.
TreeFamiTF313513.

Enzyme and pathway databases

BioCyciZFISH:HS05617-MONOMER.

Miscellaneous databases

GeneWikiiILKAP.
GenomeRNAii80895.
PROiQ9H0C8.

Gene expression databases

BgeeiENSG00000132323.
CleanExiHS_ILKAP.
ExpressionAtlasiQ9H0C8. baseline and differential.
GenevisibleiQ9H0C8. HS.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiILKAP_HUMAN
AccessioniPrimary (citable) accession number: Q9H0C8
Secondary accession number(s): B3KM39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.