Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Integrin-linked kinase-associated serine/threonine phosphatase 2C

Gene

ILKAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Enzyme regulationi

Inhibited rather than stimulated by magnesium.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi152Manganese 1By similarity1
Metal bindingi152Manganese 2By similarity1
Metal bindingi153Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi326Manganese 2By similarity1
Metal bindingi381Manganese 2By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMagnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin-linked kinase-associated serine/threonine phosphatase 2C (EC:3.1.3.16)
Short name:
ILKAP
Gene namesi
Name:ILKAP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000132323.8
HGNCiHGNC:15566 ILKAP
neXtProtiNX_Q9H0C8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi152D → A: Loss of >90% of activity. 1 Publication1
Mutagenesisi154H → D: Loss of >90% of activity. 1 Publication1
Mutagenesisi154H → L: Loss of >90% of activity. 1 Publication1

Organism-specific databases

DisGeNETi80895
OpenTargetsiENSG00000132323
PharmGKBiPA29856

Polymorphism and mutation databases

BioMutaiILKAP
DMDMi74752560

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002722711 – 392Integrin-linked kinase-associated serine/threonine phosphatase 2CAdd BLAST392

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei13PhosphoserineCombined sources1
Modified residuei210N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H0C8
MaxQBiQ9H0C8
PaxDbiQ9H0C8
PeptideAtlasiQ9H0C8
PRIDEiQ9H0C8

PTM databases

DEPODiQ9H0C8
iPTMnetiQ9H0C8
PhosphoSitePlusiQ9H0C8

Expressioni

Tissue specificityi

Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.1 Publication

Gene expression databases

BgeeiENSG00000132323
CleanExiHS_ILKAP
ExpressionAtlasiQ9H0C8 baseline and differential
GenevisibleiQ9H0C8 HS

Organism-specific databases

HPAiHPA004752
HPA056099

Interactioni

Subunit structurei

Interacts with ILK. Specific association with ILK is independent of the catalytic activity of either partner.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ILKQ134183EBI-2620298,EBI-747644

Protein-protein interaction databases

BioGridi123343, 47 interactors
IntActiQ9H0C8, 23 interactors
MINTiQ9H0C8
STRINGi9606.ENSP00000254654

Structurei

3D structure databases

ProteinModelPortaliQ9H0C8
SMRiQ9H0C8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 390PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST283

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiKOG0698 Eukaryota
COG0631 LUCA
GeneTreeiENSGT00900000140907
HOGENOMiHOG000233896
HOVERGENiHBG054286
InParanoidiQ9H0C8
KOiK17500
OMAiPDIKRCQ
OrthoDBiEOG091G0L5M
PhylomeDBiQ9H0C8
TreeFamiTF313513

Family and domain databases

CDDicd00143 PP2Cc, 1 hit
Gene3Di3.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015655 PP2C
IPR000222 PP2C_BS
IPR036457 PPM-type_dom_sf
IPR001932 PPM-type_phosphatase_dom
PANTHERiPTHR13832 PTHR13832, 1 hit
PfamiView protein in Pfam
PF00481 PP2C, 1 hit
SMARTiView protein in SMART
SM00332 PP2Cc, 1 hit
SUPFAMiSSF81606 SSF81606, 1 hit
PROSITEiView protein in PROSITE
PS01032 PPM_1, 1 hit
PS51746 PPM_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9H0C8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLFGDLPEP ERSPRPAAGK EAQKGPLLFD DLPPASSTDS GSGGPLLFDD
60 70 80 90 100
LPPASSGDSG SLATSISQMV KTEGKGAKRK TSEEEKNGSE ELVEKKVCKA
110 120 130 140 150
SSVIFGLKGY VAERKGEREE MQDAHVILND ITEECRPPSS LITRVSYFAV
160 170 180 190 200
FDGHGGIRAS KFAAQNLHQN LIRKFPKGDV ISVEKTVKRC LLDTFKHTDE
210 220 230 240 250
EFLKQASSQK PAWKDGSTAT CVLAVDNILY IANLGDSRAI LCRYNEESQK
260 270 280 290 300
HAALSLSKEH NPTQYEERMR IQKAGGNVRD GRVLGVLEVS RSIGDGQYKR
310 320 330 340 350
CGVTSVPDIR RCQLTPNDRF ILLACDGLFK VFTPEEAVNF ILSCLEDEKI
360 370 380 390
QTREGKSAAD ARYEAACNRL ANKAVQRGSA DNVTVMVVRI GH
Length:392
Mass (Da):42,907
Last modified:March 1, 2001 - v1
Checksum:iAF6ACC98508CBEA3
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06154266I → M1 PublicationCorresponds to variant dbSNP:rs34371548Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY024365 mRNA Translation: AAK07736.1
AL136850 mRNA Translation: CAB66784.1
CR533533 mRNA Translation: CAG38564.1
AK001043 mRNA Translation: BAG50851.1
AC016757 Genomic DNA Translation: AAY24336.1
BC006576 mRNA Translation: AAH06576.1
CCDSiCCDS2526.1
RefSeqiNP_110395.1, NM_030768.2
UniGeneiHs.92033

Genome annotation databases

EnsembliENST00000254654; ENSP00000254654; ENSG00000132323
GeneIDi80895
KEGGihsa:80895
UCSCiuc002vxv.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiILKAP_HUMAN
AccessioniPrimary (citable) accession number: Q9H0C8
Secondary accession number(s): B3KM39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: March 1, 2001
Last modified: March 28, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health