Q9H0B8 (CRLD2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine-rich secretory protein LCCL domain-containing 2

Alternative name(s):
Cysteine-rich secretory protein 11
Short name=CRISP-11
LCCL domain-containing cysteine-rich secretory protein 2

Gene names

Name:	CRISPLD2
Synonyms:	CRISP11, LCRISP2
ORF Names:	UNQ2914/PRO1156/PRO9783

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes matrix assembly By similarity.
Subunit structure	Binds to heparin, dermatan sulfate and chondroitin sulfate By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the CRISP family. Contains 2 LCCL domains. Contains 1 SCP domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	extracellular matrix organization Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular matrix Inferred from electronic annotation. Source: Compara extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell transport vesicle Inferred from direct assay. Source: LIFEdb
Molecular_function	heparin binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H0B8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H0B8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     436-497: TSSICKTAVH...KAFRIFAVRQ → VSRMHFQQLSRKAC

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9H0B8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     371-371: S → R
     372-497: Missing.

Isoform 4 (identifier: Q9H0B8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     81-159: TWDDELEKSA...CPERCSGPMC → VSAGSGRRGW...QWHNRHALKP
     160-497: Missing.

Note: No experimental confirmation available.

Isoform 5 (identifier: Q9H0B8-5)

The sequence of this isoform differs from the canonical sequence as follows:
     121-497: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 497	475	Cysteine-rich secretory protein LCCL domain-containing 2		PRO_0000248149
Regions
	Domain	62 – 200	139	SCP
	Domain	284 – 379	96	LCCL 1
	Domain	385 – 488	104	LCCL 2
Amino acid modifications
	Glycosylation	27	1	N-linked (GlcNAc...) Potential
	Disulfide bond	290 ↔ 308		By similarity
	Disulfide bond	312 ↔ 332		By similarity
	Disulfide bond	391 ↔ 413		By similarity
	Disulfide bond	417 ↔ 440		By similarity
Natural variations
	Alternative sequence	81 – 159	79	TWDDE…SGPMC → VSAGSGRRGWHRGWGLGHQP ALFPSQLCSPASACDGWLRV SSGRGGSRLCSVLFVCFETG SHSATDAGVQWHNRHALKP in isoform 4.		VSP_020178
	Alternative sequence	121 – 497	377	Missing in isoform 5.		VSP_020179
	Alternative sequence	160 – 497	338	Missing in isoform 4.		VSP_020180
	Alternative sequence	371	1	S → R in isoform 3.		VSP_020181
	Alternative sequence	372 – 497	126	Missing in isoform 3.		VSP_020182
	Alternative sequence	436 – 497	62	TSSIC…FAVRQ → VSRMHFQQLSRKAC in isoform 2.		VSP_020183
	Natural variant	105	1	S → G. Ref.4 Ref.5 Corresponds to variant rs12051468 [ dbSNP | Ensembl ].		VAR_027256
	Natural variant	322	1	T → S. Ref.2 Ref.5 Corresponds to variant rs721005 [ dbSNP | Ensembl ].		VAR_027257

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 1E7ADDCAB37CA4ED	FASTA	497	55,920
	10 20 30 40 50 60 MSCVLGGVIP LGLLFLVCGS QGYLLPNVTL LEELLSKYQH NESHSRVRRA IPREDKEEIL 70 80 90 100 110 120 MLHNKLRGQV QPQASNMEYM TWDDELEKSA AAWASQCIWE HGPTSLLVSI GQNLGAHWGR 130 140 150 160 170 180 YRSPGFHVQS WYDEVKDYTY PYPSECNPWC PERCSGPMCT HYTQIVWATT NKIGCAVNTC 190 200 210 220 230 240 RKMTVWGEVW ENAVYFVCNY SPKGNWIGEA PYKNGRPCSE CPPSYGGSCR NNLCYREETY 250 260 270 280 290 300 TPKPETDEMN EVETAPIPEE NHVWLQPRVM RPTKPKKTSA VNYMTQVVRC DTKMKDRCKG 310 320 330 340 350 360 STCNRYQCPA GCLNHKAKIF GTLFYESSSS ICRAAIHYGI LDDKGGLVDI TRNGKVPFFV 370 380 390 400 410 420 KSERHGVQSL SKYKPSSSFM VSKVKVQDLD CYTTVAQLCP FEKPATHCPR IHCPAHCKDE 430 440 450 460 470 480 PSYWAPVFGT NIYADTSSIC KTAVHAGVIS NESGGDVDVM PVDKKKTYVG SLRNGVQSES 490 LGTPRDGKAF RIFAVRQ
	Isoform 2 [UniParc]. Checksum: 1E597E5C8CA8CD66	FASTA	449	51,044
	10 20 30 40 50 60 MSCVLGGVIP LGLLFLVCGS QGYLLPNVTL LEELLSKYQH NESHSRVRRA IPREDKEEIL 70 80 90 100 110 120 MLHNKLRGQV QPQASNMEYM TWDDELEKSA AAWASQCIWE HGPTSLLVSI GQNLGAHWGR 130 140 150 160 170 180 YRSPGFHVQS WYDEVKDYTY PYPSECNPWC PERCSGPMCT HYTQIVWATT NKIGCAVNTC 190 200 210 220 230 240 RKMTVWGEVW ENAVYFVCNY SPKGNWIGEA PYKNGRPCSE CPPSYGGSCR NNLCYREETY 250 260 270 280 290 300 TPKPETDEMN EVETAPIPEE NHVWLQPRVM RPTKPKKTSA VNYMTQVVRC DTKMKDRCKG 310 320 330 340 350 360 STCNRYQCPA GCLNHKAKIF GTLFYESSSS ICRAAIHYGI LDDKGGLVDI TRNGKVPFFV 370 380 390 400 410 420 KSERHGVQSL SKYKPSSSFM VSKVKVQDLD CYTTVAQLCP FEKPATHCPR IHCPAHCKDE 430 440 PSYWAPVFGT NIYADVSRMH FQQLSRKAC
	Isoform 3 [UniParc]. Checksum: 8D23FBE14F53E85C	FASTA	371	42,207
	10 20 30 40 50 60 MSCVLGGVIP LGLLFLVCGS QGYLLPNVTL LEELLSKYQH NESHSRVRRA IPREDKEEIL 70 80 90 100 110 120 MLHNKLRGQV QPQASNMEYM TWDDELEKSA AAWASQCIWE HGPTSLLVSI GQNLGAHWGR 130 140 150 160 170 180 YRSPGFHVQS WYDEVKDYTY PYPSECNPWC PERCSGPMCT HYTQIVWATT NKIGCAVNTC 190 200 210 220 230 240 RKMTVWGEVW ENAVYFVCNY SPKGNWIGEA PYKNGRPCSE CPPSYGGSCR NNLCYREETY 250 260 270 280 290 300 TPKPETDEMN EVETAPIPEE NHVWLQPRVM RPTKPKKTSA VNYMTQVVRC DTKMKDRCKG 310 320 330 340 350 360 STCNRYQCPA GCLNHKAKIF GTLFYESSSS ICRAAIHYGI LDDKGGLVDI TRNGKVPFFV 370 KSERHGVQSL R
	Isoform 4 [UniParc]. Checksum: CE3BAAB490465049	FASTA	159	17,476
	10 20 30 40 50 60 MSCVLGGVIP LGLLFLVCGS QGYLLPNVTL LEELLSKYQH NESHSRVRRA IPREDKEEIL 70 80 90 100 110 120 MLHNKLRGQV QPQASNMEYM VSAGSGRRGW HRGWGLGHQP ALFPSQLCSP ASACDGWLRV 130 140 150 SSGRGGSRLC SVLFVCFETG SHSATDAGVQ WHNRHALKP
	Isoform 5 [UniParc]. Checksum: D0B40A3149B1099C	FASTA	120	13,497
	10 20 30 40 50 60 MSCVLGGVIP LGLLFLVCGS QGYLLPNVTL LEELLSKYQH NESHSRVRRA IPREDKEEIL 70 80 90 100 110 120 MLHNKLRGQV QPQASNMEYM TWDDELEKSA AAWASQCIWE HGPTSLLVSI GQNLGAHWGR

References

[1]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4), VARIANT SER-322.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Mammary gland.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLY-105.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 5), VARIANTS GLY-105 AND SER-322. Tissue: Blood and Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL136861 mRNA. Translation: CAB66795.1. AY358413 mRNA. Translation: AAQ88779.1. AY358790 mRNA. Translation: AAQ89150.1. AK027395 mRNA. Translation: BAB55081.1. CH471114 Genomic DNA. Translation: EAW95468.1. CH471114 Genomic DNA. Translation: EAW95469.1. BC007689 mRNA. Translation: AAH07689.1. BC063012 mRNA. Translation: AAH63012.1.
IPI	IPI00027821. IPI00785039. IPI00785047. IPI00785080. IPI00785102.
RefSeq	NP_113664.1. NM_031476.3.
UniGene	Hs.513779. Hs.617091.
3D structure databases
HSSP	HSSP built from PDB template 1RC9 based on UniProtKB P60623.
ProteinModelPortal	Q9H0B8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H0B8. 1 interaction.
STRING	9606.ENSP00000262424.
PTM databases
PhosphoSite	Q9H0B8.
Polymorphism databases
DMDM	74717972.
Proteomic databases
PaxDb	Q9H0B8.
PeptideAtlas	Q9H0B8.
PRIDE	Q9H0B8.
Protocols and materials databases
DNASU	83716.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000262424; ENSP00000262424; ENSG00000103196. ENST00000564567; ENSP00000457655; ENSG00000103196. ENST00000569090; ENSP00000454858; ENSG00000103196.
GeneID	83716.
KEGG	hsa:83716.
UCSC	uc002fik.4. human. uc002fil.2. human. uc002fim.2. human. uc002fin.4. human. uc010voh.1. human.
Organism-specific databases
CTD	83716.
GeneCards	GC16P084853.
HGNC	HGNC:25248. CRISPLD2.
HPA	HPA030055.
MIM	612434. gene.
neXtProt	NX_Q9H0B8.
PharmGKB	PA142672077.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2340.
HOVERGEN	HBG062505.
InParanoid	Q9H0B8.
OMA	KDKCKGS.
OrthoDB	EOG4XGZZV.
PhylomeDB	Q9H0B8.
Gene expression databases
ArrayExpress	Q9H0B8.
Bgee	Q9H0B8.
CleanEx	HS_CRISPLD2.
Genevestigator	Q9H0B8.
GermOnline	ENSG00000103196. Homo sapiens.
Family and domain databases
Gene3D	2.170.130.20. 2 hits. 3.40.33.10. 1 hit.
InterPro	IPR001283. Allrgn_V5/Tpx1. IPR018244. Allrgn_V5/Tpx1_CS. IPR014044. CAP_domain. IPR004043. LCCL. [Graphical view]
PANTHER	PTHR10334. PTHR10334. 1 hit.
Pfam	PF00188. CAP. 1 hit. PF03815. LCCL. 2 hits. [Graphical view]
PRINTS	PR00837. V5TPXLIKE.
SMART	SM00603. LCCL. 2 hits. SM00198. SCP. 1 hit. [Graphical view]
SUPFAM	SSF69848. LCCL. 2 hits. SSF55797. SCP-like_extracellular. 1 hit.
PROSITE	PS01009. CRISP_1. False negative. PS01010. CRISP_2. 1 hit. PS50820. LCCL. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CRISPLD2. human.
GenomeRNAi	83716.
NextBio	72711.
SOURCE	Search...

Entry information

Entry name

CRLD2_HUMAN

Accession

Primary (citable) accession number: Q9H0B8
Secondary accession number(s): D3DUM0 , Q6P590, Q6UWH0, Q6UXC6, Q96IB1, Q96K61

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.