Kinesin light chain 2 - Homo sapiens (Human)

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Names and origin

Protein names

Recommended name:
Kinesin light chain 2
Short name=KLC 2

Gene names

Name:

KLC2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity By similarity.

Subunit structure

Oligomeric complex composed of two heavy chains and two light chains By similarity.

Sequence similarities

Belongs to the kinesin light chain family.

Contains 6 TPR repeats.

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Ontologies

Keywords
Cellular component	Microtubule
Coding sequence diversity	Polymorphism
Domain	Coiled coil Repeat TPR repeat
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Cellular component	cytosol Inferred from sequence or structural similarity. Source: HGNC kinesin complex Inferred from sequence or structural similarity. Source: HGNC microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	microtubule motor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
YWHAG	P61981	1	EBI-726994,EBI-359832
YWHAQ	P27348	1	EBI-726994,EBI-359854

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 622

622

Kinesin light chain 2

PRO_0000215095

Regions

Repeat

198 – 231

34

TPR 1

Repeat

240 – 273

34

TPR 2

Repeat

282 – 315

34

TPR 3

Repeat

324 – 357

34

TPR 4

Repeat

366 – 399

34

TPR 5

Repeat

449 – 482

34

TPR 6

Coiled coil

78 – 143

66

Potential

Amino acid modifications

Modified residue

445

1

Phosphoserine Ref.6 Ref.7 Ref.8

Modified residue

507

1

Phosphoserine Ref.5

Modified residue

508

1

Phosphoserine Ref.5

Modified residue

582

1

Phosphoserine Ref.8 Ref.5 Ref.4

Modified residue

589

1

Phosphoserine Ref.8

Natural variations

Natural variant

517

1

P → S: dbSNP rs2276036.

VAR_020379

Experimental info

Sequence conflict

6

1

F → Y in BAB14302. Ref.2

Sequence conflict

306

1

K → R in BAB14039. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9H0B6-1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 5B57ABE4DF6E396E
Show »

FASTA

622

68,935

        10         20         30         40         50         60 
MAMMVFPREE KLSQDEIVLG TKAVIQGLET LRGEHRALLA PLVAPEAGEA EPGSQERCIL 

        70         80         90        100        110        120 
LRRSLEAIEL GLGEAQVILA LSSHLGAVES EKQKLRAQVR RLVQENQWLR EELAGTQQKL 

       130        140        150        160        170        180 
QRSEQAVAQL EEEKQHLLFM SQIRKLDEDA SPNEEKGDVP KDTLDDLFPN EDEQSPAPSP 

       190        200        210        220        230        240 
GGGDVSGQHG GYEIPARLRT LHNLVIQYAS QGRYEVAVPL CKQALEDLEK TSGHDHPDVA 

       250        260        270        280        290        300 
TMLNILALVY RDQNKYKEAA HLLNDALAIR EKTLGKDHPA VAATLNNLAV LYGKRGKYKE 

       310        320        330        340        350        360 
AEPLCKRALE IREKVLGKFH PDVAKQLSNL ALLCQNQGKA EEVEYYYRRA LEIYATRLGP 

       370        380        390        400        410        420 
DDPNVAKTKN NLASCYLKQG KYQDAETLYK EILTRAHEKE FGSVNGDNKP IWMHAEEREE 

       430        440        450        460        470        480 
SKDKRRDSAP YGEYGSWYKA CKVDSPTVNT TLRSLGALYR RQGKLEAAHT LEDCASRNRK 

       490        500        510        520        530        540 
QGLDPASQTK VVELLKDGSG RRGDRRSSRD MAGGAGPRSE SDLEDVGPTA EWNGDGSGSL 

       550        560        570        580        590        600 
RRSGSFGKLR DALRRSSEML VKKLQGGTPQ EPPNPRMKRA SSLNFLNKSV EEPTQPGGTG 

       610        620 
LSDSRTLSSS SMDLSRRSSL VG

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References

[1]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary gland and Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph.
[4]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-582, MASS SPECTROMETRY. Tissue: Epithelium.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-507; SER-508 AND SER-582, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-445, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-445, MASS SPECTROMETRY.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-445; SER-582 AND SER-589, MASS SPECTROMETRY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

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Cross-references

Sequence databases

AL136864 mRNA. Translation: CAB66798.1.
AK022449 mRNA. Translation: BAB14039.1.
AK022907 mRNA. Translation: BAB14302.1.
AK315725 mRNA. Translation: BAG38081.1.
BC034373 mRNA. Translation: AAH34373.1.

IPI

IPI00021634.

RefSeq

NP_001128246.1.
NP_001128247.1.
NP_001128248.1.
NP_073733.1.

UniGene

Hs.280792

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CEQ	X-ray	2.75	A/B	217-480	[»]
3EDT	X-ray	2.70	B/D/F/H	217-480	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9H0B6. 5 interactions.

PTM databases

PhosphoSite

Q9H0B6.

Proteomic databases

PRIDE

Q9H0B6.

Genome annotation databases

Ensembl

ENSG00000174996. Homo sapiens. [Contig view]

GeneID

64837.

UCSC

uc001ohb.1. human.

Organism-specific databases

GeneCards

GC11P065781.
GC11P065782.

H-InvDB

HIX0009825.

HGNC

HGNC:20716. KLC2.

MIM

611729. gene.

PharmGKB

PA142671587.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q9H0B6.

HOVERGEN

Q9H0B6.

OMA

Q9H0B6. PAAEWSG.

Gene expression databases

ArrayExpress

Q9H0B6.

Bgee

Q9H0B6.

CleanEx

HS_KLC2.

GermOnline

ENSG00000174996. Homo sapiens.

Family and domain databases

InterPro

IPR002151. Kinesin_light.
IPR015792. Kinesin_light_repeat.
IPR015390. Rabaptin_Rab5-bd.
IPR001440. TPR-1.
IPR011990. TPR-like_helical.
IPR013026. TPR_region.
IPR019734. TPR_repeat.
[Graphical view]

Gene3D

G3DSA:1.25.40.10. TPR-like_helical. 1 hit.

Pfam

PF09311. Rab5-bind. 1 hit.
PF00515. TPR_1. 4 hits.
[Graphical view]

PRINTS

PR00381. KINESINLIGHT.

SMART

SM00028. TPR. 4 hits.
[Graphical view]

PROSITE

PS01160. KINESIN_LIGHT. 2 hits.
PS50005. TPR. 5 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

66920.

SOURCE

Search...

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Entry information

Entry name

KLC2_HUMAN

Accession

Primary (citable) accession number: Q9H0B6
Secondary accession number(s): B2RDY4, Q9H9C8, Q9HA20

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	March 1, 2001
Last modified:	July 7, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents