Q9H0A6 (RNF32_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RING finger protein 32

Gene names

Name:	RNF32
ORF Names:	FKSG33, HSD15

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in sperm formation. Ref.1
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Highly expressed in testis, less abundant in ovary. Ref.1
Sequence similarities	Contains 1 IQ domain. Contains 2 RING-type zinc fingers.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	aggresome Inferred from direct assay Ref.1. Source: MGI endosome Inferred from direct assay. Source: LIFEdb
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FBXL12	Q9NXK8	2	EBI-724829,EBI-719790
PRAM1	Q96QH2	2	EBI-724829,EBI-2860740
STAMBPL1	Q96FJ0	2	EBI-724829,EBI-745021

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Note: Other isoforms seem to exist.

Isoform 1 (identifier: Q9H0A6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H0A6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     141-169: LLSCSHVFHKACLQAFEKFTNKKTCPLCR → FSIRG

Note: Derived from EST data.

Isoform 3 (identifier: Q9H0A6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     229-235: FTEISHR → TQDWKPA
     236-362: Missing.

Isoform 4 (identifier: Q9H0A6-5)

The sequence of this isoform differs from the canonical sequence as follows:
     170-352: Missing.

Note: No experimental confirmation available.

Isoform 5 (identifier: Q9H0A6-6)

The sequence of this isoform differs from the canonical sequence as follows:
     92-95: AQKL → GYHS
     96-362: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	RING finger protein 32		PRO_0000245532
Regions
	Domain	186 – 215	30	IQ
	Zinc finger	127 – 169	43	RING-type 1; atypical
	Zinc finger	293 – 352	60	RING-type 2; atypical
Natural variations
	Alternative sequence	92 – 95	4	AQKL → GYHS in isoform 5.		VSP_019738
	Alternative sequence	96 – 362	267	Missing in isoform 5.		VSP_019739
	Alternative sequence	141 – 169	29	LLSCS…CPLCR → FSIRG in isoform 2.		VSP_019740
	Alternative sequence	170 – 352	183	Missing in isoform 4.		VSP_019741
	Alternative sequence	229 – 235	7	FTEISHR → TQDWKPA in isoform 3.		VSP_019743
	Alternative sequence	236 – 362	127	Missing in isoform 3.		VSP_019744
	Natural variant	288	1	R → Q. Corresponds to variant rs2302148 [ dbSNP | Ensembl ].		VAR_026982
	Natural variant	291	1	H → Q. Corresponds to variant rs2302147 [ dbSNP | Ensembl ].		VAR_026983
	Natural variant	307	1	R → C. Corresponds to variant rs2302146 [ dbSNP | Ensembl ].		VAR_026984
Experimental info
	Sequence conflict	97	1	L → P in CAG38544. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 5C6AF6908FCCA456	FASTA	362	41,516
	10 20 30 40 50 60 MLKNKGHSSK KDNLAVNAVA LQDHILHDLQ LRNLSVADHS KTQVQKKENK SLKRDTKAII 70 80 90 100 110 120 DTGLKKTTQC PKLEDSEKEY VLDPKPPPLT LAQKLGLIGP PPPPLSSDEW EKVKQRSLLQ 130 140 150 160 170 180 GDSVQPCPIC KEEFELRPQV LLSCSHVFHK ACLQAFEKFT NKKTCPLCRK NQYQTRVIHD 190 200 210 220 230 240 GARLFRIKCV TRIQAYWRGC VVRKWYRNLR KTVPPTDAKL RKKFFEKKFT EISHRILCSY 250 260 270 280 290 300 NTNIEELFAE IDQCLAINRS VLQQLEEKCG HEITEEEWEK IQVQALRRET HECSICLAPL 310 320 330 340 350 360 SAAGGQRVGA GRRSREMALL SCSHVFHHAC LLALEEFSVG DRPPFHACPL CRSCYQKKIL EC
	Isoform 2 [UniParc]. Checksum: CC5A54DB27C34AF3	FASTA	338	38,741
	10 20 30 40 50 60 MLKNKGHSSK KDNLAVNAVA LQDHILHDLQ LRNLSVADHS KTQVQKKENK SLKRDTKAII 70 80 90 100 110 120 DTGLKKTTQC PKLEDSEKEY VLDPKPPPLT LAQKLGLIGP PPPPLSSDEW EKVKQRSLLQ 130 140 150 160 170 180 GDSVQPCPIC KEEFELRPQV FSIRGKNQYQ TRVIHDGARL FRIKCVTRIQ AYWRGCVVRK 190 200 210 220 230 240 WYRNLRKTVP PTDAKLRKKF FEKKFTEISH RILCSYNTNI EELFAEIDQC LAINRSVLQQ 250 260 270 280 290 300 LEEKCGHEIT EEEWEKIQVQ ALRRETHECS ICLAPLSAAG GQRVGAGRRS REMALLSCSH 310 320 330 VFHHACLLAL EEFSVGDRPP FHACPLCRSC YQKKILEC
	Isoform 3 [UniParc]. Checksum: 2D51EBF78FCCDC65	FASTA	235	27,131
	10 20 30 40 50 60 MLKNKGHSSK KDNLAVNAVA LQDHILHDLQ LRNLSVADHS KTQVQKKENK SLKRDTKAII 70 80 90 100 110 120 DTGLKKTTQC PKLEDSEKEY VLDPKPPPLT LAQKLGLIGP PPPPLSSDEW EKVKQRSLLQ 130 140 150 160 170 180 GDSVQPCPIC KEEFELRPQV LLSCSHVFHK ACLQAFEKFT NKKTCPLCRK NQYQTRVIHD 190 200 210 220 230 GARLFRIKCV TRIQAYWRGC VVRKWYRNLR KTVPPTDAKL RKKFFEKKTQ DWKPA
	Isoform 4 [UniParc]. Checksum: F1F7344CCBF8B636	FASTA	179	20,222
	10 20 30 40 50 60 MLKNKGHSSK KDNLAVNAVA LQDHILHDLQ LRNLSVADHS KTQVQKKENK SLKRDTKAII 70 80 90 100 110 120 DTGLKKTTQC PKLEDSEKEY VLDPKPPPLT LAQKLGLIGP PPPPLSSDEW EKVKQRSLLQ 130 140 150 160 170 GDSVQPCPIC KEEFELRPQV LLSCSHVFHK ACLQAFEKFT NKKTCPLCRS CYQKKILEC
	Isoform 5 [UniParc]. Checksum: 33AD8ABF2FB5FE29	FASTA	95	10,682
	10 20 30 40 50 60 MLKNKGHSSK KDNLAVNAVA LQDHILHDLQ LRNLSVADHS KTQVQKKENK SLKRDTKAII 70 80 90 DTGLKKTTQC PKLEDSEKEY VLDPKPPPLT LGYHS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A double RING-H2 domain in RNF32, a gene expressed during sperm formation." van Baren M.J., van der Linde H.C., Breedveld G.J., Baarends W.M., Rizzu P., de Graaff E., Oostra B.A., Heutink P. Biochem. Biophys. Res. Commun. 292:58-65(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 5), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
[2]	"Characterization of FKSG33, a novel gene located on human chromosome 7q35-36." Wang Y.-G., Gong L. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[4]	"A new spermatogenesis-related gene." Hu T.H., Miao S.Y., Zhang X.D., Qiao Y., Liang G., Wang L.F. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Testis.
[5]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[6]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Duodenum.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF441222 mRNA. Translation: AAM18664.1. AF441224 mRNA. Translation: AAM18666.1. AF325690 mRNA. Translation: AAG50281.1. AL136874 mRNA. Translation: CAB66808.1. AY251165 mRNA. Translation: AAP20062.1. BT007037 mRNA. Translation: AAP35686.1. CR533513 mRNA. Translation: CAG38544.1. AC005534 Genomic DNA. Translation: AAD43189.1. BC015416 mRNA. Translation: AAH15416.1.
IPI	IPI00002602. IPI00008498. IPI00760732. IPI00760842. IPI00873750.
RefSeq	NP_001171925.1. NM_001184996.1. NP_001171926.1. NM_001184997.1. NP_112198.1. NM_030936.3.
UniGene	Hs.446194.
3D structure databases
ProteinModelPortal	Q9H0A6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H0A6. 30 interactions.
MINT	MINT-1376415.
STRING	9606.ENSP00000315950.
Polymorphism databases
DMDM	74717970.
Proteomic databases
PaxDb	Q9H0A6.
PRIDE	Q9H0A6.
Protocols and materials databases
DNASU	140545.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000317955; ENSP00000315950; ENSG00000105982. ENST00000343665; ENSP00000341185; ENSG00000105982. ENST00000392740; ENSP00000376496; ENSG00000105982. ENST00000392741; ENSP00000376497; ENSG00000105982. ENST00000392743; ENSP00000376499; ENSG00000105982. ENST00000392747; ENSP00000376503; ENSG00000105982. ENST00000405335; ENSP00000385285; ENSG00000105982. ENST00000432459; ENSP00000405588; ENSG00000105982.
GeneID	140545.
KEGG	hsa:140545.
UCSC	uc003wmo.3. human. uc003wms.3. human.
Organism-specific databases
CTD	140545.
GeneCards	GC07P156433.
HGNC	HGNC:17118. RNF32.
HPA	HPA019796.
MIM	610241. gene.
neXtProt	NX_Q9H0A6.
PharmGKB	PA34435.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG270611.
HOVERGEN	HBG082459.
InParanoid	Q9H0A6.
OMA	LLSCSHM.
PhylomeDB	Q9H0A6.
Gene expression databases
ArrayExpress	Q9H0A6.
Bgee	Q9H0A6.
CleanEx	HS_RNF32.
Genevestigator	Q9H0A6.
GermOnline	ENSG00000105982. Homo sapiens.
Family and domain databases
Gene3D	3.30.40.10. 2 hits.
InterPro	IPR000048. IQ_motif_EF-hand-BS. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF00612. IQ. 1 hit. PF13639. zf-RING_2. 2 hits. [Graphical view]
SMART	SM00015. IQ. 1 hit. SM00184. RING. 2 hits. [Graphical view]
PROSITE	PS50096. IQ. 1 hit. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	140545.
NextBio	84152.
SOURCE	Search...

Entry information

Entry name

RNF32_HUMAN

Accession

Primary (citable) accession number: Q9H0A6
Secondary accession number(s): Q6FIB3 , Q6X7T4, Q8N6V8, Q8TDG0, Q96BM5, Q9Y6U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.