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Q9H0A6 (RNF32_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RING finger protein 32
Gene names
Name:RNF32
ORF Names:FKSG33, HSD15
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in sperm formation. Ref.1

Subcellular location

Cytoplasm Ref.1.

Tissue specificity

Highly expressed in testis, less abundant in ovary. Ref.1

Sequence similarities

Contains 1 IQ domain.

Contains 2 RING-type zinc fingers.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentaggresome

Inferred from direct assay Ref.1. Source: MGI

endosome

Inferred from direct assay. Source: LIFEdb

   Molecular_functionzinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Note: Other isoforms seem to exist.
Isoform 1 (identifier: Q9H0A6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9H0A6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     141-169: LLSCSHVFHKACLQAFEKFTNKKTCPLCR → FSIRG
Note: Derived from EST data.
Isoform 3 (identifier: Q9H0A6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     229-235: FTEISHR → TQDWKPA
     236-362: Missing.
Isoform 4 (identifier: Q9H0A6-5)

The sequence of this isoform differs from the canonical sequence as follows:
     170-352: Missing.
Note: No experimental confirmation available.
Isoform 5 (identifier: Q9H0A6-6)

The sequence of this isoform differs from the canonical sequence as follows:
     92-95: AQKL → GYHS
     96-362: Missing.
Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 362362RING finger protein 32
PRO_0000245532

Regions

Domain186 – 21530IQ
Zinc finger127 – 16943RING-type 1; atypical
Zinc finger293 – 35260RING-type 2; atypical

Natural variations

Alternative sequence92 – 954AQKL → GYHS in isoform 5.
VSP_019738
Alternative sequence96 – 362267Missing in isoform 5.
VSP_019739
Alternative sequence141 – 16929LLSCS…CPLCR → FSIRG in isoform 2.
VSP_019740
Alternative sequence170 – 352183Missing in isoform 4.
VSP_019741
Alternative sequence229 – 2357FTEISHR → TQDWKPA in isoform 3.
VSP_019743
Alternative sequence236 – 362127Missing in isoform 3.
VSP_019744
Natural variant2881R → Q.
Corresponds to variant rs2302148 [ dbSNP | Ensembl ].
VAR_026982
Natural variant2911H → Q.
Corresponds to variant rs2302147 [ dbSNP | Ensembl ].
VAR_026983
Natural variant3071R → C.
Corresponds to variant rs2302146 [ dbSNP | Ensembl ].
VAR_026984

Experimental info

Sequence conflict971L → P in CAG38544. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 5C6AF6908FCCA456

FASTA36241,516
        10         20         30         40         50         60 
MLKNKGHSSK KDNLAVNAVA LQDHILHDLQ LRNLSVADHS KTQVQKKENK SLKRDTKAII 

        70         80         90        100        110        120 
DTGLKKTTQC PKLEDSEKEY VLDPKPPPLT LAQKLGLIGP PPPPLSSDEW EKVKQRSLLQ 

       130        140        150        160        170        180 
GDSVQPCPIC KEEFELRPQV LLSCSHVFHK ACLQAFEKFT NKKTCPLCRK NQYQTRVIHD 

       190        200        210        220        230        240 
GARLFRIKCV TRIQAYWRGC VVRKWYRNLR KTVPPTDAKL RKKFFEKKFT EISHRILCSY 

       250        260        270        280        290        300 
NTNIEELFAE IDQCLAINRS VLQQLEEKCG HEITEEEWEK IQVQALRRET HECSICLAPL 

       310        320        330        340        350        360 
SAAGGQRVGA GRRSREMALL SCSHVFHHAC LLALEEFSVG DRPPFHACPL CRSCYQKKIL 


EC 

« Hide

Isoform 2 [UniParc].

Checksum: CC5A54DB27C34AF3
Show »

FASTA33838,741
Isoform 3 [UniParc].

Checksum: 2D51EBF78FCCDC65
Show »

FASTA23527,131
Isoform 4 [UniParc].

Checksum: F1F7344CCBF8B636
Show »

FASTA17920,222
Isoform 5 [UniParc].

Checksum: 33AD8ABF2FB5FE29
Show »

FASTA9510,682

References

« Hide 'large scale' references
[1]"A double RING-H2 domain in RNF32, a gene expressed during sperm formation."
van Baren M.J., van der Linde H.C., Breedveld G.J., Baarends W.M., Rizzu P., de Graaff E., Oostra B.A., Heutink P.
Biochem. Biophys. Res. Commun. 292:58-65(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 5), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
[2]"Characterization of FKSG33, a novel gene located on human chromosome 7q35-36."
Wang Y.-G., Gong L.
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[4]"A new spermatogenesis-related gene."
Hu T.H., Miao S.Y., Zhang X.D., Qiao Y., Liang G., Wang L.F.
Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Tissue: Testis.
[5]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[6]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Duodenum.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF441222 mRNA. Translation: AAM18664.1.
AF441224 mRNA. Translation: AAM18666.1.
AF325690 mRNA. Translation: AAG50281.1.
AL136874 mRNA. Translation: CAB66808.1.
AY251165 mRNA. Translation: AAP20062.1.
BT007037 mRNA. Translation: AAP35686.1.
CR533513 mRNA. Translation: CAG38544.1.
AC005534 Genomic DNA. Translation: AAD43189.1.
BC015416 mRNA. Translation: AAH15416.1.
RefSeqNP_001171925.1. NM_001184996.1.
NP_001171926.1. NM_001184997.1.
NP_112198.1. NM_030936.3.
XP_005249579.1. XM_005249522.2.
UniGeneHs.446194.

3D structure databases

ProteinModelPortalQ9H0A6.
SMRQ9H0A6. Positions 125-206, 291-360.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid126622. 43 interactions.
IntActQ9H0A6. 30 interactions.
MINTMINT-1376415.
STRING9606.ENSP00000315950.

Polymorphism databases

DMDM74717970.

Proteomic databases

PaxDbQ9H0A6.
PRIDEQ9H0A6.

Protocols and materials databases

DNASU140545.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000317955; ENSP00000315950; ENSG00000105982. [Q9H0A6-1]
ENST00000343665; ENSP00000341185; ENSG00000105982. [Q9H0A6-2]
ENST00000392740; ENSP00000376496; ENSG00000105982. [Q9H0A6-6]
ENST00000392741; ENSP00000376497; ENSG00000105982. [Q9H0A6-4]
ENST00000392743; ENSP00000376499; ENSG00000105982. [Q9H0A6-1]
ENST00000392747; ENSP00000376503; ENSG00000105982. [Q9H0A6-6]
ENST00000405335; ENSP00000385285; ENSG00000105982. [Q9H0A6-1]
ENST00000432459; ENSP00000405588; ENSG00000105982. [Q9H0A6-1]
GeneID140545.
KEGGhsa:140545.
UCSCuc003wmo.3. human. [Q9H0A6-1]
uc003wms.3. human. [Q9H0A6-4]

Organism-specific databases

CTD140545.
GeneCardsGC07P156433.
HGNCHGNC:17118. RNF32.
HPAHPA019796.
MIM610241. gene.
neXtProtNX_Q9H0A6.
PharmGKBPA34435.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG270611.
HOVERGENHBG082459.
InParanoidQ9H0A6.
OMACSHMFHH.
PhylomeDBQ9H0A6.
TreeFamTF329796.

Gene expression databases

ArrayExpressQ9H0A6.
BgeeQ9H0A6.
CleanExHS_RNF32.
GenevestigatorQ9H0A6.

Family and domain databases

Gene3D3.30.40.10. 2 hits.
InterProIPR000048. IQ_motif_EF-hand-BS.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF00612. IQ. 1 hit.
PF13639. zf-RING_2. 2 hits.
[Graphical view]
SMARTSM00015. IQ. 1 hit.
SM00184. RING. 2 hits.
[Graphical view]
PROSITEPS50096. IQ. 1 hit.
PS50089. ZF_RING_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiRNF32.
GenomeRNAi140545.
NextBio84152.
PROQ9H0A6.
SOURCESearch...

Entry information

Entry nameRNF32_HUMAN
AccessionPrimary (citable) accession number: Q9H0A6
Secondary accession number(s): Q6FIB3 expand/collapse secondary AC list , Q6X7T4, Q8N6V8, Q8TDG0, Q96BM5, Q9Y6U1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM