Q9H0A0 (NAT10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyltransferase 10
EC=2.3.1.-

Gene names

Name:	NAT10
Synonyms:	ALP, KIAA1709

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1025 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division. Ref.6 Ref.8 Ref.9 Ref.12
Subunit structure	Interacts with SUN1 (via N-terminus). Also interacts with TERT. Ref.8 Ref.9
Subcellular location	Nucleus › nucleolus. Note: Nucleolar in interphase and redistributes to the perichromosomal layer and to the midbody during telophase. Ref.6 Ref.7 Ref.12
Induction	Transcriptionally activated by genotoxic agents; possible role in DNA damage and induction of cellular resistance to genotoxic agents. Ref.10
Sequence similarities	Belongs to the UPF0202 family. Contains 1 N-acetyltransferase domain.
Sequence caution	The sequence AAO49126.1 differs from that shown. Reason: Frameshift at position 981. The sequence BAB21800.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Acyltransferase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW N-acetyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LYAR	Q9NX58	1	EBI-876527,EBI-713507

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1025	1025	N-acetyltransferase 10		PRO_0000215883
Regions
	Domain	558 – 753	196	N-acetyltransferase
	Nucleotide binding	284 – 291	8	ATP Potential
	Region	702 – 1025	324	Required for localization to the nucleolus and midbody
	Compositional bias	989 – 1025	37	Lys-rich
Amino acid modifications
	Modified residue	426	1	N6-acetyllysine Ref.13
	Modified residue	934	1	Phosphoserine Ref.11
	Modified residue	984	1	Phosphoserine Ref.11
	Modified residue	987	1	Phosphoserine Ref.11
Natural variations
	Natural variant	461	1	Y → H. Ref.1 Ref.2 Ref.3 Ref.5 Ref.6 Ref.14 Corresponds to variant rs2957516 [ dbSNP | Ensembl ].		VAR_059858
	Natural variant	983	1	A → T. Corresponds to variant rs36006049 [ dbSNP | Ensembl ].		VAR_061894
Experimental info
	Sequence conflict	26	1	F → L in BAA91800. Ref.3
	Sequence conflict	162	1	T → A in AAO49126. Ref.6
	Sequence conflict	315	1	S → C in BAB13995. Ref.3
	Sequence conflict	879	1	Q → R in BAB13995. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9H0A0 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: 88734933A46EB0EE	FASTA	1,025	115,730
	10 20 30 40 50 60 MHRKKVDNRI RILIENGVAE RQRSLFVVVG DRGKDQVVIL HHMLSKATVK ARPSVLWCYK 70 80 90 100 110 120 KELGFSSHRK KRMRQLQKKI KNGTLNIKQD DPFELFIAAT NIRYCYYNET HKILGNTFGM 130 140 150 160 170 180 CVLQDFEALT PNLLARTVET VEGGGLVVIL LRTMNSLKQL YTVTMDVHSR YRTEAHQDVV 190 200 210 220 230 240 GRFNERFILS LASCKKCLVI DDQLNILPIS SHVATMEALP PQTPDESLGP SDLELRELKE 250 260 270 280 290 300 SLQDTQPVGV LVDCCKTLDQ AKAVLKFIEG ISEKTLRSTV ALTAARGRGK SAALGLAIAG 310 320 330 340 350 360 AVAFGYSNIF VTSPSPDNLH TLFEFVFKGF DALQYQEHLD YEIIQSLNPE FNKAVIRVNV 370 380 390 400 410 420 FREHRQTIQY IHPADAVKLG QAELVVIDEA AAIPLPLVKS LLGPYLVFMA STINGYEGTG 430 440 450 460 470 480 RSLSLKLIQQ LRQQSAQSQV STTAENKTTT TARLASARTL YEVSLQESIR YAPGDAVEKW 490 500 510 520 530 540 LNDLLCLDCL NITRIVSGCP LPEACELYYV NRDTLFCYHK ASEVFLQRLM ALYVASHYKN 550 560 570 580 590 600 SPNDLQMLSD APAHHLFCLL PPVPPTQNAL PEVLAVIQVC LEGEISRQSI LNSLSRGKKA 610 620 630 640 650 660 SGDLIPWTVS EQFQDPDFGG LSGGRVVRIA VHPDYQGMGY GSRALQLLQM YYEGRFPCLE 670 680 690 700 710 720 EKVLETPQEI HTVSSEAVSL LEEVITPRKD LPPLLLKLNE RPAERLDYLG VSYGLTPRLL 730 740 750 760 770 780 KFWKRAGFVP VYLRQTPNDL TGEHSCIMLK TLTDEDEADQ GGWLAAFWKD FRRRFLALLS 790 800 810 820 830 840 YQFSTFSPSL ALNIIQNRNM GKPAQPALSR EELEALFLPY DLKRLEMYSR NMVDYHLIMD 850 860 870 880 890 900 MIPAISRIYF LNQLGDLALS AAQSALLLGI GLQHKSVDQL EKEIELPSGQ LMGLFNRIIR 910 920 930 940 950 960 KVVKLFNEVQ EKAIEEQMVA AKDVVMEPTM KTLSDDLDEA AKEFQEKHKK EVGKLKSMDL 970 980 990 1000 1010 1020 SEYIIRGDDE EWNEVLNKAG PNASIISLKS DKKRKLEAKQ EPKQSKKLKN RETKNKKDMK LKRKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Hattori A., Kondo Y., Okumura K., Ohara O. DNA Res. 7:347-355(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT HIS-461. Tissue: Brain.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT HIS-461. Tissue: Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT HIS-461. Tissue: Mammary gland.
[4]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT HIS-461. Tissue: Brain.
[6]	"Molecular cloning of a novel human gene encoding histone acetyltransferase-like protein involved in transcriptional activation of hTERT." Lv J., Liu H., Wang Q., Tang Z., Hou L., Zhang B. Biochem. Biophys. Res. Commun. 311:506-513(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 154-1025, FUNCTION, SUBCELLULAR LOCATION, VARIANT HIS-461. Tissue: Cervix carcinoma.
[7]	"Functional proteomic analysis of human nucleolus." Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J. Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Histone acetyltransferase hALP and nuclear membrane protein hsSUN1 function in de-condensation of mitotic chromosomes." Chi Y.-H., Haller K., Peloponese J.-M. Jr., Jeang K.-T. J. Biol. Chem. 282:27447-27458(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SUN1.
[9]	"Purification of human telomerase complexes identifies factors involved in telomerase biogenesis and telomere length regulation." Fu D., Collins K. Mol. Cell 28:773-785(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TERT.
[10]	"DNA damage induces N-acetyltransferase NAT10 gene expression through transcriptional activation." Liu H., Ling Y., Gong Y., Sun Y., Hou L., Zhang B. Mol. Cell. Biochem. 300:249-258(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY GENOTOXIC AGENTS.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-934; SER-984 AND SER-987, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"NAT10, a nucleolar protein, localizes to the midbody and regulates cytokinesis and acetylation of microtubules." Shen Q., Zheng X., McNutt M.A., Guang L., Sun Y., Wang J., Gong Y., Hou L., Zhang B. Exp. Cell Res. 315:1653-1667(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[13]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-426, MASS SPECTROMETRY.
[14]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] HIS-461, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB051496 mRNA. Translation: BAB21800.1. Different initiation. AL136882 mRNA. Translation: CAB66816.1. AK001636 mRNA. Translation: BAA91800.1. AK022241 mRNA. Translation: BAB13995.1. AC090469 Genomic DNA. No translation available. BC035558 mRNA. Translation: AAH35558.1. AF489535 mRNA. Translation: AAO49126.1. Frameshift.
IPI	IPI00300127.
RefSeq	NP_001137502.1. NM_001144030.1. NP_078938.2. NM_024662.2.
UniGene	Hs.577281.
3D structure databases
ProteinModelPortal	Q9H0A0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H0A0. 6 interactions.
MINT	MINT-2817086.
STRING	9606.ENSP00000257829.
PTM databases
PhosphoSite	Q9H0A0.
2D gel databases
SWISS-2DPAGE	Q9H0A0.
Proteomic databases
PaxDb	Q9H0A0.
PRIDE	Q9H0A0.
Protocols and materials databases
DNASU	55226.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000257829; ENSP00000257829; ENSG00000135372.
GeneID	55226.
KEGG	hsa:55226.
UCSC	uc001mvk.3. human.
Organism-specific databases
CTD	55226.
GeneCards	GC11P034084.
HGNC	HGNC:29830. NAT10.
HPA	CAB035546.
MIM	609221. gene.
neXtProt	NX_Q9H0A0.
PharmGKB	PA143485555.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1444.
HOGENOM	HOG000210833.
HOVERGEN	HBG052206.
InParanoid	Q9H0A0.
KO	K14521.
OMA	LSAFWKD.
OrthoDB	EOG4H9XJS.
PhylomeDB	Q9H0A0.
Gene expression databases
ArrayExpress	Q9H0A0.
Bgee	Q9H0A0.
CleanEx	HS_NAT10.
Genevestigator	Q9H0A0.
GermOnline	ENSG00000135372. Homo sapiens.
Family and domain databases
Gene3D	3.40.630.30. 2 hits.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR013562. DUF1726. IPR000182. GNAT_dom. IPR007807. Helicase_dom. [Graphical view]
Pfam	PF08351. DUF1726. 1 hit. PF05127. Helicase_RecD. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NAT10. human.
GenomeRNAi	55226.
NextBio	59220.
SOURCE	Search...

Entry information

Entry name

NAT10_HUMAN

Accession

Primary (citable) accession number: Q9H0A0
Secondary accession number(s): Q86WK5 , Q9C0F4, Q9HA61, Q9NVF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	November 30, 2010
Last modified:	May 29, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.