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Protein

NUAK family SNF1-like kinase 2

Gene

NUAK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactori

Mg2+2 Publications

Enzyme regulationi

Activated by phosphorylation on Thr-208.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81ATPPROSITE-ProRule annotation1 Publication1
Active sitei175Proton acceptorPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi59 – 67ATPPROSITE-ProRule annotationBy similarity9

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  • actin cytoskeleton organization Source: UniProtKB
  • apoptotic process Source: UniProtKB-KW
  • cellular response to glucose starvation Source: UniProtKB
  • intracellular signal transduction Source: GO_Central
  • negative regulation of apoptotic process Source: UniProtKB
  • protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS08878-MONOMER.
SignaLinkiQ9H093.
SIGNORiQ9H093.

Names & Taxonomyi

Protein namesi
Recommended name:
NUAK family SNF1-like kinase 2 (EC:2.7.11.1)
Alternative name(s):
Omphalocele kinase 2
SNF1/AMP kinase-related kinase
Short name:
SNARK
Gene namesi
Name:NUAK2Imported
Synonyms:OMPHK2, SNARK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:29558. NUAK2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi81K → R: Loss of autophosphorylation, kinase activity and of anti-apoptotic activity. 1 Publication1
Mutagenesisi208T → A: Prevents phosphorylation and activation by STK11/LKB1 complex. 1 Publication1

Organism-specific databases

DisGeNETi81788.
PharmGKBiPA142671243.

Chemistry databases

ChEMBLiCHEMBL5698.
GuidetoPHARMACOLOGYi2130.

Polymorphism and mutation databases

BioMutaiNUAK2.
DMDMi74761376.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002477561 – 628NUAK family SNF1-like kinase 2Add BLAST628

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei208Phosphothreonine; by LKB11 Publication1
Modified residuei435PhosphoserineCombined sources1
Modified residuei523PhosphoserineCombined sources1
Modified residuei544PhosphoserineCombined sources1
Modified residuei547PhosphoserineCombined sources1
Modified residuei573PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Autophosphorylated in vitro.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9H093.
MaxQBiQ9H093.
PaxDbiQ9H093.
PeptideAtlasiQ9H093.
PRIDEiQ9H093.

PTM databases

iPTMnetiQ9H093.
PhosphoSitePlusiQ9H093.

Expressioni

Gene expression databases

BgeeiENSG00000163545.
CleanExiHS_NUAK2.
ExpressionAtlasiQ9H093. baseline and differential.
GenevisibleiQ9H093. HS.

Organism-specific databases

HPAiHPA008958.

Interactioni

Protein-protein interaction databases

BioGridi123581. 17 interactors.
IntActiQ9H093. 3 interactors.
MINTiMINT-4719062.
STRINGi9606.ENSP00000356125.

Chemistry databases

BindingDBiQ9H093.

Structurei

3D structure databases

ProteinModelPortaliQ9H093.
SMRiQ9H093.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini53 – 303Protein kinasePROSITE-ProRule annotationAdd BLAST251

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0611. Eukaryota.
ENOG410XPDM. LUCA.
HOGENOMiHOG000004864.
HOVERGENiHBG007160.
InParanoidiQ9H093.
KOiK08800.
OMAiTDCQEVT.
OrthoDBiEOG091G033W.
PhylomeDBiQ9H093.
TreeFamiTF324572.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLVFARRS GPTPSAAELA RPLAEGLIKS PKPLMKKQAV KRHHHKHNLR
60 70 80 90 100
HRYEFLETLG KGTYGKVKKA RESSGRLVAI KSIRKDKIKD EQDLMHIRRE
110 120 130 140 150
IEIMSSLNHP HIIAIHEVFE NSSKIVIVME YASRGDLYDY ISERQQLSER
160 170 180 190 200
EARHFFRQIV SAVHYCHQNR VVHRDLKLEN ILLDANGNIK IADFGLSNLY
210 220 230 240 250
HQGKFLQTFC GSPLYASPEI VNGKPYTGPE VDSWSLGVLL YILVHGTMPF
260 270 280 290 300
DGHDHKILVK QISNGAYREP PKPSDACGLI RWLLMVNPTR RATLEDVASH
310 320 330 340 350
WWVNWGYATR VGEQEAPHEG GHPGSDSARA SMADWLRRSS RPLLENGAKV
360 370 380 390 400
CSFFKQHAPG GGSTTPGLER QHSLKKSRKE NDMAQSLHSD TADDTAHRPG
410 420 430 440 450
KSNLKLPKGI LKKKVSASAE GVQEDPPELS PIPASPGQAA PLLPKKGILK
460 470 480 490 500
KPRQRESGYY SSPEPSESGE LLDAGDVFVS GDPKEQKPPQ ASGLLLHRKG
510 520 530 540 550
ILKLNGKFSQ TALELAAPTT FGSLDELAPP RPLARASRPS GAVSEDSILS
560 570 580 590 600
SESFDQLDLP ERLPEPPLRG CVSVDNLTGL EEPPSEGPGS CLRRWRQDPL
610 620
GDSCFSLTDC QEVTATYRQA LRVCSKLT
Length:628
Mass (Da):69,612
Last modified:March 1, 2001 - v1
Checksum:iF76F8B1BF94F4C87
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_040964309T → S.1 PublicationCorresponds to variant rs55745939dbSNPEnsembl.1
Natural variantiVAR_040965341R → L.1 PublicationCorresponds to variant rs35208615dbSNPEnsembl.1
Natural variantiVAR_040966503K → R in an ovarian Endometrioid carcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_040967516A → V.1 PublicationCorresponds to variant rs35070935dbSNPEnsembl.1
Natural variantiVAR_040968541G → E in a breast pleomorphic lobular carcinoma sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136891 mRNA. Translation: CAB66825.1.
AK074830 mRNA. Translation: BAC11234.1.
BC017306 mRNA. Translation: AAH17306.1.
RefSeqiNP_112214.2. NM_030952.2.
UniGeneiHs.497512.

Genome annotation databases

EnsembliENST00000367157; ENSP00000356125; ENSG00000163545.
GeneIDi81788.
KEGGihsa:81788.
UCSCiuc001hce.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136891 mRNA. Translation: CAB66825.1.
AK074830 mRNA. Translation: BAC11234.1.
BC017306 mRNA. Translation: AAH17306.1.
RefSeqiNP_112214.2. NM_030952.2.
UniGeneiHs.497512.

3D structure databases

ProteinModelPortaliQ9H093.
SMRiQ9H093.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123581. 17 interactors.
IntActiQ9H093. 3 interactors.
MINTiMINT-4719062.
STRINGi9606.ENSP00000356125.

Chemistry databases

BindingDBiQ9H093.
ChEMBLiCHEMBL5698.
GuidetoPHARMACOLOGYi2130.

PTM databases

iPTMnetiQ9H093.
PhosphoSitePlusiQ9H093.

Polymorphism and mutation databases

BioMutaiNUAK2.
DMDMi74761376.

Proteomic databases

EPDiQ9H093.
MaxQBiQ9H093.
PaxDbiQ9H093.
PeptideAtlasiQ9H093.
PRIDEiQ9H093.

Protocols and materials databases

DNASUi81788.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367157; ENSP00000356125; ENSG00000163545.
GeneIDi81788.
KEGGihsa:81788.
UCSCiuc001hce.4. human.

Organism-specific databases

CTDi81788.
DisGeNETi81788.
GeneCardsiNUAK2.
HGNCiHGNC:29558. NUAK2.
HPAiHPA008958.
MIMi608131. gene.
neXtProtiNX_Q9H093.
PharmGKBiPA142671243.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0611. Eukaryota.
ENOG410XPDM. LUCA.
HOGENOMiHOG000004864.
HOVERGENiHBG007160.
InParanoidiQ9H093.
KOiK08800.
OMAiTDCQEVT.
OrthoDBiEOG091G033W.
PhylomeDBiQ9H093.
TreeFamiTF324572.

Enzyme and pathway databases

BioCyciZFISH:HS08878-MONOMER.
SignaLinkiQ9H093.
SIGNORiQ9H093.

Miscellaneous databases

GeneWikiiNUAK2.
GenomeRNAii81788.
PROiQ9H093.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163545.
CleanExiHS_NUAK2.
ExpressionAtlasiQ9H093. baseline and differential.
GenevisibleiQ9H093. HS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUAK2_HUMAN
AccessioniPrimary (citable) accession number: Q9H093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.