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Q9H081

- MIS12_HUMAN

UniProt

Q9H081 - MIS12_HUMAN

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Protein

Protein MIS12 homolog

Gene

MIS12

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis.3 Publications

GO - Biological processi

  1. chromosome segregation Source: UniProtKB
  2. kinetochore assembly Source: UniProtKB
  3. mitotic cell cycle Source: Reactome
  4. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Enzyme and pathway databases

ReactomeiREACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.
REACT_682. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MIS12 homolog
Gene namesi
Name:MIS12Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:24967. MIS12.

Subcellular locationi

Nucleus. Chromosomecentromerekinetochore
Note: Associated with the kinetochore.

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. MIS12/MIND type complex Source: UniProtKB
  3. nucleus Source: LIFEdb
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134951024.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Protein MIS12 homologPRO_0000248234Add
BLAST

Proteomic databases

MaxQBiQ9H081.
PaxDbiQ9H081.
PRIDEiQ9H081.

PTM databases

PhosphoSiteiQ9H081.

Expressioni

Gene expression databases

BgeeiQ9H081.
CleanExiHS_MIS12.
ExpressionAtlasiQ9H081. baseline and differential.
GenevestigatoriQ9H081.

Organism-specific databases

HPAiHPA044482.

Interactioni

Subunit structurei

Component of the MIS12 complex composed of MIS12, DSN1, NSL1 and PMF1. Also interacts with CASC5, CBX3, CBX5, NDC80 and ZWINT.1 Publication

Protein-protein interaction databases

BioGridi122474. 32 interactions.
IntActiQ9H081. 26 interactions.
MINTiMINT-4718912.
STRINGi9606.ENSP00000370557.

Structurei

3D structure databases

ProteinModelPortaliQ9H081.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili108 – 20598Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the mis12 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG45566.
GeneTreeiENSGT00390000018665.
HOGENOMiHOG000251597.
HOVERGENiHBG055269.
InParanoidiQ9H081.
KOiK11543.
OMAiPMAYEAQ.
PhylomeDBiQ9H081.
TreeFamiTF101136.

Family and domain databases

InterProiIPR008685. Centromere_Mis12.
[Graphical view]
PfamiPF05859. Mis12. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9H081-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVDPMTYEA QFFGFTPQTC MLRIYIAFQD YLFEVMQAVE QVILKKLDGI
60 70 80 90 100
PDCDISPVQI RKCTEKFLCF MKGHFDNLFS KMEQLFLQLI LRIPSNILLP
110 120 130 140 150
EDKCKETPYS EEDFQHLQKE IEQLQEKYKT ELCTKQALLA ELEEQKIVQA
160 170 180 190 200
KLKQTLTFFD ELHNVGRDHG TSDFRESLVS LVQNSRKLQN IRDNVEKESK

RLKIS
Length:205
Mass (Da):24,140
Last modified:March 1, 2001 - v1
Checksum:i8CC906E843D4BD56
GO

Sequence cautioni

The sequence AK056072 differs from that shown. Reason: Frameshift at position 147.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti21 – 211M → V.
Corresponds to variant rs16954781 [ dbSNP | Ensembl ].
VAR_034106

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL136906 mRNA. Translation: CAB66840.1.
AK056072 mRNA. No translation available.
CR533460 mRNA. Translation: CAG38491.1.
BC000229 mRNA. Translation: AAH00229.1.
CCDSiCCDS11074.1.
RefSeqiNP_001245146.1. NM_001258217.1.
NP_001245147.1. NM_001258218.1.
NP_001245148.1. NM_001258219.1.
NP_001245149.1. NM_001258220.1.
NP_076944.1. NM_024039.2.
XP_005256854.1. XM_005256797.2.
XP_005256855.1. XM_005256798.2.
XP_006721638.1. XM_006721575.1.
UniGeneiHs.267194.

Genome annotation databases

EnsembliENST00000381165; ENSP00000370557; ENSG00000167842.
ENST00000573759; ENSP00000461252; ENSG00000167842.
ENST00000611091; ENSP00000484532; ENSG00000167842.
GeneIDi79003.
KEGGihsa:79003.
UCSCiuc002gcd.4. human.

Polymorphism databases

DMDMi74733516.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL136906 mRNA. Translation: CAB66840.1 .
AK056072 mRNA. No translation available.
CR533460 mRNA. Translation: CAG38491.1 .
BC000229 mRNA. Translation: AAH00229.1 .
CCDSi CCDS11074.1.
RefSeqi NP_001245146.1. NM_001258217.1.
NP_001245147.1. NM_001258218.1.
NP_001245148.1. NM_001258219.1.
NP_001245149.1. NM_001258220.1.
NP_076944.1. NM_024039.2.
XP_005256854.1. XM_005256797.2.
XP_005256855.1. XM_005256798.2.
XP_006721638.1. XM_006721575.1.
UniGenei Hs.267194.

3D structure databases

ProteinModelPortali Q9H081.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 122474. 32 interactions.
IntActi Q9H081. 26 interactions.
MINTi MINT-4718912.
STRINGi 9606.ENSP00000370557.

PTM databases

PhosphoSitei Q9H081.

Polymorphism databases

DMDMi 74733516.

Proteomic databases

MaxQBi Q9H081.
PaxDbi Q9H081.
PRIDEi Q9H081.

Protocols and materials databases

DNASUi 79003.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000381165 ; ENSP00000370557 ; ENSG00000167842 .
ENST00000573759 ; ENSP00000461252 ; ENSG00000167842 .
ENST00000611091 ; ENSP00000484532 ; ENSG00000167842 .
GeneIDi 79003.
KEGGi hsa:79003.
UCSCi uc002gcd.4. human.

Organism-specific databases

CTDi 79003.
GeneCardsi GC17P005390.
HGNCi HGNC:24967. MIS12.
HPAi HPA044482.
MIMi 609178. gene.
neXtProti NX_Q9H081.
PharmGKBi PA134951024.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG45566.
GeneTreei ENSGT00390000018665.
HOGENOMi HOG000251597.
HOVERGENi HBG055269.
InParanoidi Q9H081.
KOi K11543.
OMAi PMAYEAQ.
PhylomeDBi Q9H081.
TreeFami TF101136.

Enzyme and pathway databases

Reactomei REACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.
REACT_682. Mitotic Prometaphase.

Miscellaneous databases

GeneWikii MIS12.
GenomeRNAii 79003.
NextBioi 67625.
PROi Q9H081.
SOURCEi Search...

Gene expression databases

Bgeei Q9H081.
CleanExi HS_MIS12.
ExpressionAtlasi Q9H081. baseline and differential.
Genevestigatori Q9H081.

Family and domain databases

InterProi IPR008685. Centromere_Mis12.
[Graphical view ]
Pfami PF05859. Mis12. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: TestisImported.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: EyeImported.
  5. "Human centromere chromatin protein hMis12, essential for equal segregation, is independent of CENP-A loading pathway."
    Goshima G., Kiyomitsu T., Yoda K., Yanagida M.
    J. Cell Biol. 160:25-39(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1."
    Obuse C., Iwasaki O., Kiyomitsu T., Goshima G., Toyoda Y., Yanagida M.
    Nat. Cell Biol. 6:1135-1141(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CASC5; CBX3; CBX5; DSN1; NDC80; NSL1; PMF1 AND ZWINT, SUBCELLULAR LOCATION.
  7. "The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation."
    Kline S.L., Cheeseman I.M., Hori T., Fukagawa T., Desai A.
    J. Cell Biol. 173:9-17(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, COMPONENT OF MIS12 COMPLEX, SUBCELLULAR LOCATION.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMIS12_HUMAN
AccessioniPrimary (citable) accession number: Q9H081
Secondary accession number(s): Q96N24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3