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Protein

Caseinolytic peptidase B protein homolog

Gene

CLPB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May function as a regulatory ATPase and be related to secretion/protein trafficking process.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi381 – 3888ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular response to heat Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Caseinolytic peptidase B protein homolog
Alternative name(s):
Suppressor of potassium transport defect 3
Gene namesi
Name:CLPB
Synonyms:HSP78, SKD3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:30664. CLPB.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: Ensembl
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142672092.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 707707Caseinolytic peptidase B protein homologPRO_0000191239Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei589 – 5891N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9H078.
PaxDbiQ9H078.
PRIDEiQ9H078.

PTM databases

PhosphoSiteiQ9H078.

Expressioni

Gene expression databases

BgeeiQ9H078.
CleanExiHS_CLPB.
ExpressionAtlasiQ9H078. baseline and differential.
GenevestigatoriQ9H078.

Organism-specific databases

HPAiHPA039005.
HPA039006.

Interactioni

Protein-protein interaction databases

BioGridi123531. 16 interactions.
IntActiQ9H078. 15 interactions.
MINTiMINT-1196059.
STRINGi9606.ENSP00000294053.

Structurei

3D structure databases

ProteinModelPortaliQ9H078.
SMRiQ9H078. Positions 93-306, 339-645.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati133 – 16230ANK 1Add
BLAST
Repeati166 – 19530ANK 2Add
BLAST
Repeati265 – 29531ANK 3Add
BLAST
Repeati298 – 32730ANK 4Add
BLAST

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated
Contains 4 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00390000012961.
HOGENOMiHOG000006543.
HOVERGENiHBG025978.
InParanoidiQ9H078.
KOiK03695.
OrthoDBiEOG769ZJM.
PhylomeDBiQ9H078.
TreeFamiTF328654.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR001270. ClpA/B.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF07724. AAA_2. 1 hit.
PF00023. Ank. 1 hit.
PF12796. Ank_2. 1 hit.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 1 hit.
SM00248. ANK. 3 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 2 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9H078-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLGSLVLRRK ALAPRLLLRL LRSPTLRGHG GASGRNVTTG SLGEPQWLRV
60 70 80 90 100
ATGGRPGTSP ALFSGRGAAT GGRQGGRFDT KCLAAATWGR LPGPEETLPG
110 120 130 140 150
QDSWNGVPSR AGLGMCALAA ALVVHCYSKS PSNKDAALLE AARANNMQEV
160 170 180 190 200
SRLLSEGADV NAKHRLGWTA LMVAAINRNN SVVQVLLAAG ADPNLGDDFS
210 220 230 240 250
SVYKTAKEQG IHSLEDGGQD GASRHITNQW TSALEFRRWL GLPAGVLITR
260 270 280 290 300
EDDFNNRLNN RASFKGCTAL HYAVLADDYR TVKELLDGGA NPLQRNEMGH
310 320 330 340 350
TPLDYAREGE VMKLLRTSEA KYQEKQRKRE AEERRRFPLE QRLKEHIIGQ
360 370 380 390 400
ESAIATVGAA IRRKENGWYD EEHPLVFLFL GSSGIGKTEL AKQTAKYMHK
410 420 430 440 450
DAKKGFIRLD MSEFQERHEV AKFIGSPPGY VGHEEGGQLT KKLKQCPNAV
460 470 480 490 500
VLFDEVDKAH PDVLTIMLQL FDEGRLTDGK GKTIDCKDAI FIMTSNVASD
510 520 530 540 550
EIAQHALQLR QEALEMSRNR IAENLGDVQI SDKITISKNF KENVIRPILK
560 570 580 590 600
AHFRRDEFLG RINEIVYFLP FCHSELIQLV NKELNFWAKR AKQRHNITLL
610 620 630 640 650
WDREVADVLV DGYNVHYGAR SIKHEVERRV VNQLAAAYEQ DLLPGGCTLR
660 670 680 690 700
ITVEDSDKQL LKSPELPSPQ AEKRLPKLRL EIIDKDSKTR RLDIRAPLHP

EKVCNTI
Length:707
Mass (Da):78,729
Last modified:March 1, 2001 - v1
Checksum:i0E0F2A244CA20635
GO
Isoform 2 (identifier: Q9H078-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     216-245: Missing.

Note: No experimental confirmation available.

Show »
Length:677
Mass (Da):75,449
Checksum:iC03F18C94337B9E4
GO
Isoform 3 (identifier: Q9H078-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     152-180: Missing.
     216-245: Missing.

Note: No experimental confirmation available.

Show »
Length:648
Mass (Da):72,276
Checksum:i586F3100E877B4D4
GO
Isoform 4 (identifier: Q9H078-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-151: MLGSLVLRRK...ARANNMQEVS → MPRGCHLGTP...TKISSSEWPL

Note: No experimental confirmation available.

Show »
Length:662
Mass (Da):74,543
Checksum:iF9DE15704672A495
GO
Isoform 5 (identifier: Q9H078-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-171: Missing.
     216-245: Missing.

Note: No experimental confirmation available.

Show »
Length:506
Mass (Da):57,664
Checksum:i55E4A602D8B4F44A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti413 – 4131E → K in BAG63459. (PubMed:14702039)Curated
Sequence conflicti563 – 5631N → S in BAG63409. (PubMed:14702039)Curated
Sequence conflicti650 – 6501R → C in BAG63459. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti295 – 2951R → T.
Corresponds to variant rs7938203 [ dbSNP | Ensembl ].
VAR_048740

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 171171Missing in isoform 5. 1 PublicationVSP_057397Add
BLAST
Alternative sequencei1 – 151151MLGSL…MQEVS → MPRGCHLGTPSWSRRNTPRT GQLERGPQQGRTGHVRPGRS AGGSLLQQESVQQGCSPVGS CPCQQYARSQQPQETAKNDA QSRSWAGLNAGVSLKNTKIS SSEWPL in isoform 4. 1 PublicationVSP_044725Add
BLAST
Alternative sequencei152 – 18029Missing in isoform 3. 1 PublicationVSP_044726Add
BLAST
Alternative sequencei216 – 24530Missing in isoform 2, isoform 3 and isoform 5. 1 PublicationVSP_001106Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136909 mRNA. Translation: CAB66843.1.
AL834484 mRNA. Translation: CAD39142.1.
AK023214 mRNA. Translation: BAB14467.1.
AK302006 mRNA. Translation: BAG63409.1.
AK302069 mRNA. Translation: BAG63459.1.
AK302158 mRNA. Translation: BAG63526.1.
AP000593 Genomic DNA. No translation available.
AP002892 Genomic DNA. No translation available.
AP003785 Genomic DNA. No translation available.
BC006404 mRNA. Translation: AAH06404.1.
CCDSiCCDS58152.1. [Q9H078-4]
CCDS58153.1. [Q9H078-3]
CCDS58154.1. [Q9H078-2]
CCDS8215.1. [Q9H078-1]
RefSeqiNP_001245321.1. NM_001258392.1. [Q9H078-2]
NP_001245322.1. NM_001258393.1. [Q9H078-3]
NP_001245323.1. NM_001258394.1. [Q9H078-4]
NP_110440.1. NM_030813.4. [Q9H078-1]
UniGeneiHs.523877.

Genome annotation databases

EnsembliENST00000294053; ENSP00000294053; ENSG00000162129. [Q9H078-1]
ENST00000340729; ENSP00000340385; ENSG00000162129. [Q9H078-3]
ENST00000437826; ENSP00000407296; ENSG00000162129. [Q9H078-4]
ENST00000538039; ENSP00000441518; ENSG00000162129. [Q9H078-2]
GeneIDi81570.
KEGGihsa:81570.
UCSCiuc001osj.4. human. [Q9H078-1]
uc001osk.4. human. [Q9H078-2]

Polymorphism databases

DMDMi25009267.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136909 mRNA. Translation: CAB66843.1.
AL834484 mRNA. Translation: CAD39142.1.
AK023214 mRNA. Translation: BAB14467.1.
AK302006 mRNA. Translation: BAG63409.1.
AK302069 mRNA. Translation: BAG63459.1.
AK302158 mRNA. Translation: BAG63526.1.
AP000593 Genomic DNA. No translation available.
AP002892 Genomic DNA. No translation available.
AP003785 Genomic DNA. No translation available.
BC006404 mRNA. Translation: AAH06404.1.
CCDSiCCDS58152.1. [Q9H078-4]
CCDS58153.1. [Q9H078-3]
CCDS58154.1. [Q9H078-2]
CCDS8215.1. [Q9H078-1]
RefSeqiNP_001245321.1. NM_001258392.1. [Q9H078-2]
NP_001245322.1. NM_001258393.1. [Q9H078-3]
NP_001245323.1. NM_001258394.1. [Q9H078-4]
NP_110440.1. NM_030813.4. [Q9H078-1]
UniGeneiHs.523877.

3D structure databases

ProteinModelPortaliQ9H078.
SMRiQ9H078. Positions 93-306, 339-645.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123531. 16 interactions.
IntActiQ9H078. 15 interactions.
MINTiMINT-1196059.
STRINGi9606.ENSP00000294053.

PTM databases

PhosphoSiteiQ9H078.

Polymorphism databases

DMDMi25009267.

Proteomic databases

MaxQBiQ9H078.
PaxDbiQ9H078.
PRIDEiQ9H078.

Protocols and materials databases

DNASUi81570.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000294053; ENSP00000294053; ENSG00000162129. [Q9H078-1]
ENST00000340729; ENSP00000340385; ENSG00000162129. [Q9H078-3]
ENST00000437826; ENSP00000407296; ENSG00000162129. [Q9H078-4]
ENST00000538039; ENSP00000441518; ENSG00000162129. [Q9H078-2]
GeneIDi81570.
KEGGihsa:81570.
UCSCiuc001osj.4. human. [Q9H078-1]
uc001osk.4. human. [Q9H078-2]

Organism-specific databases

CTDi81570.
GeneCardsiGC11M072008.
HGNCiHGNC:30664. CLPB.
HPAiHPA039005.
HPA039006.
neXtProtiNX_Q9H078.
PharmGKBiPA142672092.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00390000012961.
HOGENOMiHOG000006543.
HOVERGENiHBG025978.
InParanoidiQ9H078.
KOiK03695.
OrthoDBiEOG769ZJM.
PhylomeDBiQ9H078.
TreeFamiTF328654.

Miscellaneous databases

ChiTaRSiCLPB. human.
GeneWikiiCLPB.
GenomeRNAii81570.
NextBioi71868.
PROiQ9H078.

Gene expression databases

BgeeiQ9H078.
CleanExiHS_CLPB.
ExpressionAtlasiQ9H078. baseline and differential.
GenevestigatoriQ9H078.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR001270. ClpA/B.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF07724. AAA_2. 1 hit.
PF00023. Ank. 1 hit.
PF12796. Ank_2. 1 hit.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 1 hit.
SM00248. ANK. 3 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 2 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3; 4 AND 5).
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Uterus.
  5. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-589, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCLPB_HUMAN
AccessioniPrimary (citable) accession number: Q9H078
Secondary accession number(s): B4DXJ7
, B4DXP7, B4DXW4, E7EWN6, F8W7P6, Q8ND11, Q9H8Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: March 1, 2001
Last modified: February 4, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.