Q9H078 (CLPB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Caseinolytic peptidase B protein homolog

Alternative name(s):
Suppressor of potassium transport defect 3

Gene names

Name:	CLPB
Synonyms:	HSP78, SKD3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	707 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as a regulatory ATPase and be related to secretion/protein trafficking process.
Sequence similarities	Belongs to the clpA/clpB family. Contains 4 ANK repeats.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	ANK repeat Repeat
Ligand	ATP-binding Nucleotide-binding
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to heat Inferred from direct assay PubMed 2745427. Source: UniProtKB
Cellular_component	mitochondrion Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H078-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H078-2)

The sequence of this isoform differs from the canonical sequence as follows:
     216-245: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9H078-3)

The sequence of this isoform differs from the canonical sequence as follows:
     152-180: Missing.
     216-245: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q9H078-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-151: MLGSLVLRRK...ARANNMQEVS → MPRGCHLGTP...TKISSSEWPL

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 707	707	Caseinolytic peptidase B protein homolog		PRO_0000191239
Regions
	Repeat	133 – 162	30	ANK 1
	Repeat	166 – 195	30	ANK 2
	Repeat	265 – 295	31	ANK 3
	Repeat	298 – 327	30	ANK 4
	Nucleotide binding	381 – 388	8	ATP Potential
Amino acid modifications
	Modified residue	589	1	N6-acetyllysine Ref.5
Natural variations
	Alternative sequence	1 – 151	151	MLGSL…MQEVS → MPRGCHLGTPSWSRRNTPRT GQLERGPQQGRTGHVRPGRS AGGSLLQQESVQQGCSPVGS CPCQQYARSQQPQETAKNDA QSRSWAGLNAGVSLKNTKIS SSEWPL in isoform 4.		VSP_044725
	Alternative sequence	152 – 180	29	Missing in isoform 3.		VSP_044726
	Alternative sequence	216 – 245	30	Missing in isoform 2 and isoform 3.		VSP_001106
	Natural variant	295	1	R → T. Corresponds to variant rs7938203 [ dbSNP | Ensembl ].		VAR_048740
Experimental info
	Sequence conflict	413	1	E → K in BAG63459. Ref.2
	Sequence conflict	563	1	N → S in BAG63409. Ref.2
	Sequence conflict	650	1	R → C in BAG63459. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 0E0F2A244CA20635	FASTA	707	78,729
	10 20 30 40 50 60 MLGSLVLRRK ALAPRLLLRL LRSPTLRGHG GASGRNVTTG SLGEPQWLRV ATGGRPGTSP 70 80 90 100 110 120 ALFSGRGAAT GGRQGGRFDT KCLAAATWGR LPGPEETLPG QDSWNGVPSR AGLGMCALAA 130 140 150 160 170 180 ALVVHCYSKS PSNKDAALLE AARANNMQEV SRLLSEGADV NAKHRLGWTA LMVAAINRNN 190 200 210 220 230 240 SVVQVLLAAG ADPNLGDDFS SVYKTAKEQG IHSLEDGGQD GASRHITNQW TSALEFRRWL 250 260 270 280 290 300 GLPAGVLITR EDDFNNRLNN RASFKGCTAL HYAVLADDYR TVKELLDGGA NPLQRNEMGH 310 320 330 340 350 360 TPLDYAREGE VMKLLRTSEA KYQEKQRKRE AEERRRFPLE QRLKEHIIGQ ESAIATVGAA 370 380 390 400 410 420 IRRKENGWYD EEHPLVFLFL GSSGIGKTEL AKQTAKYMHK DAKKGFIRLD MSEFQERHEV 430 440 450 460 470 480 AKFIGSPPGY VGHEEGGQLT KKLKQCPNAV VLFDEVDKAH PDVLTIMLQL FDEGRLTDGK 490 500 510 520 530 540 GKTIDCKDAI FIMTSNVASD EIAQHALQLR QEALEMSRNR IAENLGDVQI SDKITISKNF 550 560 570 580 590 600 KENVIRPILK AHFRRDEFLG RINEIVYFLP FCHSELIQLV NKELNFWAKR AKQRHNITLL 610 620 630 640 650 660 WDREVADVLV DGYNVHYGAR SIKHEVERRV VNQLAAAYEQ DLLPGGCTLR ITVEDSDKQL 670 680 690 700 LKSPELPSPQ AEKRLPKLRL EIIDKDSKTR RLDIRAPLHP EKVCNTI
	Isoform 2 [UniParc]. Checksum: C03F18C94337B9E4	FASTA	677	75,449
	10 20 30 40 50 60 MLGSLVLRRK ALAPRLLLRL LRSPTLRGHG GASGRNVTTG SLGEPQWLRV ATGGRPGTSP 70 80 90 100 110 120 ALFSGRGAAT GGRQGGRFDT KCLAAATWGR LPGPEETLPG QDSWNGVPSR AGLGMCALAA 130 140 150 160 170 180 ALVVHCYSKS PSNKDAALLE AARANNMQEV SRLLSEGADV NAKHRLGWTA LMVAAINRNN 190 200 210 220 230 240 SVVQVLLAAG ADPNLGDDFS SVYKTAKEQG IHSLEVLITR EDDFNNRLNN RASFKGCTAL 250 260 270 280 290 300 HYAVLADDYR TVKELLDGGA NPLQRNEMGH TPLDYAREGE VMKLLRTSEA KYQEKQRKRE 310 320 330 340 350 360 AEERRRFPLE QRLKEHIIGQ ESAIATVGAA IRRKENGWYD EEHPLVFLFL GSSGIGKTEL 370 380 390 400 410 420 AKQTAKYMHK DAKKGFIRLD MSEFQERHEV AKFIGSPPGY VGHEEGGQLT KKLKQCPNAV 430 440 450 460 470 480 VLFDEVDKAH PDVLTIMLQL FDEGRLTDGK GKTIDCKDAI FIMTSNVASD EIAQHALQLR 490 500 510 520 530 540 QEALEMSRNR IAENLGDVQI SDKITISKNF KENVIRPILK AHFRRDEFLG RINEIVYFLP 550 560 570 580 590 600 FCHSELIQLV NKELNFWAKR AKQRHNITLL WDREVADVLV DGYNVHYGAR SIKHEVERRV 610 620 630 640 650 660 VNQLAAAYEQ DLLPGGCTLR ITVEDSDKQL LKSPELPSPQ AEKRLPKLRL EIIDKDSKTR 670 RLDIRAPLHP EKVCNTI
	Isoform 3 [UniParc]. Checksum: 586F3100E877B4D4	FASTA	648	72,276
	10 20 30 40 50 60 MLGSLVLRRK ALAPRLLLRL LRSPTLRGHG GASGRNVTTG SLGEPQWLRV ATGGRPGTSP 70 80 90 100 110 120 ALFSGRGAAT GGRQGGRFDT KCLAAATWGR LPGPEETLPG QDSWNGVPSR AGLGMCALAA 130 140 150 160 170 180 ALVVHCYSKS PSNKDAALLE AARANNMQEV SSVVQVLLAA GADPNLGDDF SSVYKTAKEQ 190 200 210 220 230 240 GIHSLEVLIT REDDFNNRLN NRASFKGCTA LHYAVLADDY RTVKELLDGG ANPLQRNEMG 250 260 270 280 290 300 HTPLDYAREG EVMKLLRTSE AKYQEKQRKR EAEERRRFPL EQRLKEHIIG QESAIATVGA 310 320 330 340 350 360 AIRRKENGWY DEEHPLVFLF LGSSGIGKTE LAKQTAKYMH KDAKKGFIRL DMSEFQERHE 370 380 390 400 410 420 VAKFIGSPPG YVGHEEGGQL TKKLKQCPNA VVLFDEVDKA HPDVLTIMLQ LFDEGRLTDG 430 440 450 460 470 480 KGKTIDCKDA IFIMTSNVAS DEIAQHALQL RQEALEMSRN RIAENLGDVQ ISDKITISKN 490 500 510 520 530 540 FKENVIRPIL KAHFRRDEFL GRINEIVYFL PFCHSELIQL VNKELNFWAK RAKQRHNITL 550 560 570 580 590 600 LWDREVADVL VDGYNVHYGA RSIKHEVERR VVNQLAAAYE QDLLPGGCTL RITVEDSDKQ 610 620 630 640 LLKSPELPSP QAEKRLPKLR LEIIDKDSKT RRLDIRAPLH PEKVCNTI
	Isoform 4 [UniParc]. Checksum: F9DE15704672A495	FASTA	662	74,543
	10 20 30 40 50 60 MPRGCHLGTP SWSRRNTPRT GQLERGPQQG RTGHVRPGRS AGGSLLQQES VQQGCSPVGS 70 80 90 100 110 120 CPCQQYARSQ QPQETAKNDA QSRSWAGLNA GVSLKNTKIS SSEWPLRLLS EGADVNAKHR 130 140 150 160 170 180 LGWTALMVAA INRNNSVVQV LLAAGADPNL GDDFSSVYKT AKEQGIHSLE DGGQDGASRH 190 200 210 220 230 240 ITNQWTSALE FRRWLGLPAG VLITREDDFN NRLNNRASFK GCTALHYAVL ADDYRTVKEL 250 260 270 280 290 300 LDGGANPLQR NEMGHTPLDY AREGEVMKLL RTSEAKYQEK QRKREAEERR RFPLEQRLKE 310 320 330 340 350 360 HIIGQESAIA TVGAAIRRKE NGWYDEEHPL VFLFLGSSGI GKTELAKQTA KYMHKDAKKG 370 380 390 400 410 420 FIRLDMSEFQ ERHEVAKFIG SPPGYVGHEE GGQLTKKLKQ CPNAVVLFDE VDKAHPDVLT 430 440 450 460 470 480 IMLQLFDEGR LTDGKGKTID CKDAIFIMTS NVASDEIAQH ALQLRQEALE MSRNRIAENL 490 500 510 520 530 540 GDVQISDKIT ISKNFKENVI RPILKAHFRR DEFLGRINEI VYFLPFCHSE LIQLVNKELN 550 560 570 580 590 600 FWAKRAKQRH NITLLWDREV ADVLVDGYNV HYGARSIKHE VERRVVNQLA AAYEQDLLPG 610 620 630 640 650 660 GCTLRITVED SDKQLLKSPE LPSPQAEKRL PKLRLEIIDK DSKTRRLDIR APLHPEKVCN TI

References

[1]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4). Tissue: Testis.
[3]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[5]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-589, MASS SPECTROMETRY.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL136909 mRNA. Translation: CAB66843.1. AL834484 mRNA. Translation: CAD39142.1. AK023214 mRNA. Translation: BAB14467.1. AK302006 mRNA. Translation: BAG63409.1. AK302069 mRNA. Translation: BAG63459.1. AP000593 Genomic DNA. No translation available. AP002892 Genomic DNA. No translation available. AP003785 Genomic DNA. No translation available. BC006404 mRNA. Translation: AAH06404.1.
IPI	IPI00006615. IPI00216192. IPI00910365. IPI01014146.
RefSeq	NP_001245321.1. NM_001258392.1. NP_001245322.1. NM_001258393.1. NP_001245323.1. NM_001258394.1. NP_110440.1. NM_030813.4.
UniGene	Hs.523877.
3D structure databases
ProteinModelPortal	Q9H078.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9H078. 12 interactions.
MINT	MINT-1196059.
STRING	9606.ENSP00000294053.
PTM databases
PhosphoSite	Q9H078.
Polymorphism databases
DMDM	25009267.
Proteomic databases
PaxDb	Q9H078.
PRIDE	Q9H078.
Protocols and materials databases
DNASU	81570.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000294053; ENSP00000294053; ENSG00000162129. ENST00000340729; ENSP00000340385; ENSG00000162129. ENST00000437826; ENSP00000407296; ENSG00000162129. ENST00000538039; ENSP00000441518; ENSG00000162129.
GeneID	81570.
KEGG	hsa:81570.
UCSC	uc001osj.3. human. uc001osk.3. human.
Organism-specific databases
CTD	81570.
GeneCards	GC11M072008.
HGNC	HGNC:30664. CLPB.
HPA	HPA039006.
neXtProt	NX_Q9H078.
PharmGKB	PA142672092.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0666.
HOGENOM	HOG000006543.
HOVERGEN	HBG025978.
InParanoid	Q9H078.
KO	K03695.
OrthoDB	EOG4KKZ2V.
PhylomeDB	Q9H078.
Gene expression databases
ArrayExpress	Q9H078.
Bgee	Q9H078.
CleanEx	HS_CLPB.
Genevestigator	Q9H078.
GermOnline	ENSG00000162129. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.20. 2 hits.
InterPro	IPR003593. AAA+_ATPase. IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR013093. ATPase_AAA-2. IPR001270. Chaprnin_ClpA/B. IPR019489. Clp_ATPase_C. [Graphical view]
Pfam	PF07724. AAA_2. 1 hit. PF12796. Ank_2. 1 hit. PF10431. ClpB_D2-small. 1 hit. [Graphical view]
PRINTS	PR00300. CLPPROTEASEA.
SMART	SM00382. AAA. 1 hit. SM00248. ANK. 3 hits. SM01086. ClpB_D2-small. 1 hit. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 3 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	81570.
NextBio	71868.

Entry information

Entry name

CLPB_HUMAN

Accession

Primary (citable) accession number: Q9H078
Secondary accession number(s): B4DXJ7 , B4DXP7, E7EWN6, F8W7P6, Q8ND11, Q9H8Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.