Polyadenylate-binding protein-interacting protein 1

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

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Names and origin

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General annotation (Comments)

Ontologies

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Alternative products

Sequence annotation (Features)

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Entry

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IntAct

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Reviewed, UniProtKB/Swiss-Prot Q9H074 (PAIP1_HUMAN)

Last modified February 9, 2010. Version 78. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Protein names

Recommended name:
    Polyadenylate-binding protein-interacting protein 1
      Short name=Poly(A)-binding protein-interacting protein 1
      Short name=PABP-interacting protein 1
      Short name=PAIP-1

Gene names

Name:

PAIP1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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Function	Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Ref.1 Ref.4
Subunit structure	Interacts with the RRM1-RRM2 and C-terminus regions of PABPC1 in a 1:1 stoichiometry. Interacts with EIF4A. Ref.1 Ref.5 Ref.6 Ref.7
Subcellular location	Cytoplasm Probable.
Domain	Only the PABPC1-interacting motif-1 (PAM1) stimulates translation initiation.
Sequence similarities	Contains 1 MIF4G domain.

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Keywords
Biological process	Translation regulation
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	mRNA stabilization Ref.7 Traceable author statement. Source: UniProtKB regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW translational initiation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Ref.1 Traceable author statement. Source: ProtInc
Molecular function	RNA binding Ref.1 Traceable author statement. Source: ProtInc protein binding Ref.1 Ref.5 Ref.7 Inferred from physical interaction. Source: UniProtKB translation activator activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

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With	Entry	#Exp.	IntAct	Notes
PABPC1	P11940	3	EBI-81519,EBI-81531

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Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 479

479

Polyadenylate-binding protein-interacting protein 1

PRO_0000058177

Domain

159 – 376

218

MIF4G

Region

116 – 143

PABPC1-interacting motif-2 (PAM2)

Region

440 – 479

PABPC1-interacting motif-1 (PAM1)

Compositional bias

10 – 36

Gly-rich

Compositional bias

45 – 98

Pro-rich

Alternative sequence

10 – 88

Missing in isoform 2.

VSP_010005

Sequence conflict

239

R → C in AAH15937. Ref.3

479

Helix Strand Turn

Details...

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Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: A4820607190A3A43 Show »	FASTA	479	53,525
10 20 30 40 50 60 MSDGFDRAPG AGRGRSRGLG RGGGGPEGGG FPNGAGPAER ARHQPPQPKA PGFLQPPPLR 70 80 90 100 110 120 QPRTTPPPGA QCEVPASPQR PSRPGALPEQ TRPLRAPPSS QDKIPQQNSE SAMAKPQVVV 130 140 150 160 170 180 APVLMSKLSV NAPEFYPSGY SSSYTESYED GCEDYPTLSE YVQDFLNHLT EQPGSFETEI 190 200 210 220 230 240 EQFAETLNGC VTTDDALQEL VELIYQQATS IPNFSYMGAR LCNYLSHHLT ISPQSGNFRQ 250 260 270 280 290 300 LLLQRCRTEY EVKDQAAKGD EVTRKRFHAF VLFLGELYLN LEIKGTNGQV TRADILQVGL 310 320 330 340 350 360 RELLNALFSN PMDDNLICAV KLLKLTGSVL EDAWKEKGKM DMEEIIQRIE NVVLDANCSR 370 380 390 400 410 420 DVKQMLLKLV ELRSSNWGRV HATSTYREAT PENDPNYFMN EPTFYTSDGV PFTAADPDYQ 430 440 450 460 470 EKYQELLERE DFFPDYEENG TDLSGAGDPY LDDIDDEMDP EIEEAYEKFC LESERKRKQ « Hide
Isoform 2. Checksum: 9743587A6F54BA25 Show »	FASTA	400	45,631
10 20 30 40 50 60 MSDGFDRAPE QTRPLRAPPS SQDKIPQQNS ESAMAKPQVV VAPVLMSKLS VNAPEFYPSG 70 80 90 100 110 120 YSSSYTESYE DGCEDYPTLS EYVQDFLNHL TEQPGSFETE IEQFAETLNG CVTTDDALQE 130 140 150 160 170 180 LVELIYQQAT SIPNFSYMGA RLCNYLSHHL TISPQSGNFR QLLLQRCRTE YEVKDQAAKG 190 200 210 220 230 240 DEVTRKRFHA FVLFLGELYL NLEIKGTNGQ VTRADILQVG LRELLNALFS NPMDDNLICA 250 260 270 280 290 300 VKLLKLTGSV LEDAWKEKGK MDMEEIIQRI ENVVLDANCS RDVKQMLLKL VELRSSNWGR 310 320 330 340 350 360 VHATSTYREA TPENDPNYFM NEPTFYTSDG VPFTAADPDY QEKYQELLER EDFFPDYEEN 370 380 390 400 GTDLSGAGDP YLDDIDDEMD PEIEEAYEKF CLESERKRKQ « Hide

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	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation." Craig A.W.B., Haghighat A., Yu A.T.K., Sonenberg N. Nature 392:520-523(1998) [PubMed: 9548260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION IN TRANSLATION INITIATION STIMULATION, INTERACTION WITH PABPC1 AND EIF4A. Tissue: Placenta.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Kidney and Skin.
[4]	"A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex." Grosset C., Chen C.-Y.A., Xu N., Sonenberg N., Jacquemin-Sablon H., Shyu A.-B. Cell 103:29-40(2000) [PubMed: 11051545] [Abstract] Cited for: FUNCTION IN TRANSLATIONALLY COUPLED MRNA TURNOVER, IDENTIFICATION IN A COMPLEX WITH HNRPD; SYNCRIP; PABPC1 AND UNR.
[5]	"Multiple portions of poly(A)-binding protein stimulate translation in vivo." Gray N.K., Coller J.M., Dickson K.S., Wickens M. EMBO J. 19:4723-4733(2000) [PubMed: 10970864] [Abstract] Cited for: INTERACTION WITH PABPC1.
[6]	"Translational repression by a novel partner of human poly(A) binding protein, Paip2." Khaleghpour K., Svitkin Y.V., Craig A.W.B., DeMaria C.T., Deo R.C., Burley S.K., Sonenberg N. Mol. Cell 7:205-216(2001) [PubMed: 11172725] [Abstract] Cited for: INTERACTION WITH PABPC1.
[7]	"Paip1 interacts with poly(A) binding protein through two independent binding motifs." Roy G., De Crescenzo G., Khaleghpour K., Kahvejian A., O'Connor-McCourt M., Sonenberg N. Mol. Cell. Biol. 22:3769-3782(2002) [PubMed: 11997512] [Abstract] Cited for: INTERACTION WITH PABPC1.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

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EMBL
GenBank
DDBJ

AF013758 mRNA. Translation: AAC39697.2.
AL136920 mRNA. Translation: CAB66854.1.
BC005295 mRNA. Translation: AAH05295.1.
BC015937 mRNA. Translation: AAH15937.1.

IPI

IPI00021466.
IPI00337775.

RefSeq

NP_006442.2.
NP_877590.1.
NP_899152.1.

UniGene

Hs.482038

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JH4	NMR	-	B	124-144	[»]

SMR

Q9H074. Positions 172-386.

ModBase

Search...

IntAct

Q9H074. 1 interaction.

STRING

Q9H074.

PhosphoSite

Q9H074.

PRIDE

Q9H074.

Ensembl

ENST00000306846; ENSP00000302768; ENSG00000172239; Homo sapiens. [Genome view]

GeneID

10605.

KEGG

hsa:10605.

UCSC

uc003job.1. human.

CTD

10605.

GeneCards

GC05M043562.
GC05M043563.
GC17P018489.

H-InvDB

HIX0004847.

HGNC

HGNC:16945. PAIP1.

MIM

605184. gene.

PharmGKB

PA134941557.

GenAtlas

Search...

eggNOG

prNOG18463.

HOGENOM

HBG713446.

HOVERGEN

Q9H074.

InParanoid

Q9H074.

OMA

LNGWVTT.

PhylomeDB

Q9H074.

Reactome

REACT_20605. Metabolism of mRNA.

ArrayExpress

Q9H074.

Bgee

Q9H074.

CleanEx

HS_PAIP1.

Genevestigator

Q9H074.

GermOnline

ENSG00000172239. Homo sapiens.

InterPro

IPR016024. ARM-type_fold.
IPR009818. Ataxin-2_C.
IPR016021. MIF4-like_typ_1/2/3.
IPR003890. MIF4G-like_typ-3.
[Graphical view]

Gene3D

G3DSA:1.25.40.180. MIF4-like_typ_1/2/3. 1 hit.

Pfam

PF02854. MIF4G. 1 hit.
PF07145. PAM2. 1 hit.
[Graphical view]

SMART

SM00543. MIF4G. 1 hit.
[Graphical view]

ProtoNet

Search...

NextBio

40270.

SOURCE

Search...

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Entry name

PAIP1_HUMAN

Accession

Primary (citable) accession number: Q9H074
Secondary accession number(s): O60455, Q96B61, Q9BS63

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H074-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H074-2)

The sequence of this isoform differs from the canonical sequence as follows:
10-88: Missing.