Q9H074 (PAIP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyadenylate-binding protein-interacting protein 1
Short name=PABP-interacting protein 1
Short name=PAIP-1
Short name=Poly(A)-binding protein-interacting protein 1

Gene names

Name:

PAIP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Ref.1 Ref.4
Subunit structure	Interacts with the RRM1-RRM2 and C-terminus regions of PABPC1 in a 1:1 stoichiometry. Interacts with EIF4A. Ref.1 Ref.4 Ref.5 Ref.6 Ref.7
Subcellular location	Cytoplasm Probable.
Domain	Only the PABPC1-interacting motif-1 (PAM1) stimulates translation initiation.
Sequence similarities	Contains 1 MIF4G domain.

Ontologies

Keywords
Biological process	Translation regulation
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA stabilization Traceable author statement Ref.7. Source: UniProtKB nuclear-transcribed mRNA poly(A) tail shortening Traceable author statement. Source: Reactome translational initiation Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro RNA binding Traceable author statement Ref.1. Source: ProtInc translation activator activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PABPC1	P11940	6	EBI-81519,EBI-81531

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9H074-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9H074-2)

The sequence of this isoform differs from the canonical sequence as follows:
     10-88: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 479

479

Polyadenylate-binding protein-interacting protein 1

PRO_0000058177

Regions

Domain

159 – 376

218

MIF4G

Region

116 – 143

28

PABPC1-interacting motif-2 (PAM2)

Region

440 – 479

40

PABPC1-interacting motif-1 (PAM1)

Compositional bias

10 – 36

27

Gly-rich

Compositional bias

45 – 98

54

Pro-rich

Natural variations

Alternative sequence

10 – 88

79

Missing in isoform 2.

VSP_010005

Experimental info

Sequence conflict

239

1

R → C in AAH15937. Ref.3

Secondary structure

1

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479

Helix Strand Turn

Details...

Helix

158 – 171

14

Helix

173 – 175

3

Helix

176 – 190

15

Helix

194 – 209

16

Helix

215 – 228

14

Helix

238 – 250

13

Helix

253 – 256

4

Helix

261 – 280

20

Beta strand

288 – 291

4

Helix

294 – 309

16

Helix

313 – 336

24

Helix

341 – 355

15

Helix

360 – 372

13

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: A4820607190A3A43	FASTA	479	53,525
	10 20 30 40 50 60 MSDGFDRAPG AGRGRSRGLG RGGGGPEGGG FPNGAGPAER ARHQPPQPKA PGFLQPPPLR 70 80 90 100 110 120 QPRTTPPPGA QCEVPASPQR PSRPGALPEQ TRPLRAPPSS QDKIPQQNSE SAMAKPQVVV 130 140 150 160 170 180 APVLMSKLSV NAPEFYPSGY SSSYTESYED GCEDYPTLSE YVQDFLNHLT EQPGSFETEI 190 200 210 220 230 240 EQFAETLNGC VTTDDALQEL VELIYQQATS IPNFSYMGAR LCNYLSHHLT ISPQSGNFRQ 250 260 270 280 290 300 LLLQRCRTEY EVKDQAAKGD EVTRKRFHAF VLFLGELYLN LEIKGTNGQV TRADILQVGL 310 320 330 340 350 360 RELLNALFSN PMDDNLICAV KLLKLTGSVL EDAWKEKGKM DMEEIIQRIE NVVLDANCSR 370 380 390 400 410 420 DVKQMLLKLV ELRSSNWGRV HATSTYREAT PENDPNYFMN EPTFYTSDGV PFTAADPDYQ 430 440 450 460 470 EKYQELLERE DFFPDYEENG TDLSGAGDPY LDDIDDEMDP EIEEAYEKFC LESERKRKQ
	Isoform 2 [UniParc]. Checksum: 9743587A6F54BA25	FASTA	400	45,631
	10 20 30 40 50 60 MSDGFDRAPE QTRPLRAPPS SQDKIPQQNS ESAMAKPQVV VAPVLMSKLS VNAPEFYPSG 70 80 90 100 110 120 YSSSYTESYE DGCEDYPTLS EYVQDFLNHL TEQPGSFETE IEQFAETLNG CVTTDDALQE 130 140 150 160 170 180 LVELIYQQAT SIPNFSYMGA RLCNYLSHHL TISPQSGNFR QLLLQRCRTE YEVKDQAAKG 190 200 210 220 230 240 DEVTRKRFHA FVLFLGELYL NLEIKGTNGQ VTRADILQVG LRELLNALFS NPMDDNLICA 250 260 270 280 290 300 VKLLKLTGSV LEDAWKEKGK MDMEEIIQRI ENVVLDANCS RDVKQMLLKL VELRSSNWGR 310 320 330 340 350 360 VHATSTYREA TPENDPNYFM NEPTFYTSDG VPFTAADPDY QEKYQELLER EDFFPDYEEN 370 380 390 400 GTDLSGAGDP YLDDIDDEMD PEIEEAYEKF CLESERKRKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation." Craig A.W.B., Haghighat A., Yu A.T.K., Sonenberg N. Nature 392:520-523(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION IN TRANSLATION INITIATION STIMULATION, INTERACTION WITH PABPC1 AND EIF4A. Tissue: Placenta.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Kidney and Skin.
[4]	"A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex." Grosset C., Chen C.-Y.A., Xu N., Sonenberg N., Jacquemin-Sablon H., Shyu A.-B. Cell 103:29-40(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN TRANSLATIONALLY COUPLED MRNA TURNOVER, IDENTIFICATION IN A COMPLEX WITH HNRPD; SYNCRIP; PABPC1 AND UNR.
[5]	"Multiple portions of poly(A)-binding protein stimulate translation in vivo." Gray N.K., Coller J.M., Dickson K.S., Wickens M. EMBO J. 19:4723-4733(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PABPC1.
[6]	"Translational repression by a novel partner of human poly(A) binding protein, Paip2." Khaleghpour K., Svitkin Y.V., Craig A.W.B., DeMaria C.T., Deo R.C., Burley S.K., Sonenberg N. Mol. Cell 7:205-216(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PABPC1.
[7]	"Paip1 interacts with poly(A) binding protein through two independent binding motifs." Roy G., De Crescenzo G., Khaleghpour K., Kahvejian A., O'Connor-McCourt M., Sonenberg N. Mol. Cell. Biol. 22:3769-3782(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PABPC1.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF013758 mRNA. Translation: AAC39697.2.
AL136920 mRNA. Translation: CAB66854.1.
BC005295 mRNA. Translation: AAH05295.1.
BC015937 mRNA. Translation: AAH15937.1.

IPI

IPI00021466.
IPI00337775.

RefSeq

NP_006442.2. NM_006451.4.
NP_877590.1. NM_182789.3.
NP_899152.1. NM_183323.2.

UniGene

Hs.482038.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JH4	NMR	-	B	124-144	[»]
3NTW	X-ray	2.60	B/D	123-144	[»]
3RK6	X-ray	2.00	A/B	157-373	[»]

ProteinModelPortal

Q9H074.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9H074. 4 interactions.

STRING

9606.ENSP00000302768.

PTM databases

PhosphoSite

Q9H074.

Polymorphism databases

DMDM

46397025.

Proteomic databases

PaxDb

Q9H074.

PRIDE

Q9H074.

Protocols and materials databases

DNASU

10605.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000306846; ENSP00000302768; ENSG00000172239.
ENST00000436644; ENSP00000387729; ENSG00000172239.

GeneID

10605.

KEGG

hsa:10605.

UCSC

uc003joa.3. human.
uc003job.3. human.

Organism-specific databases

CTD

10605.

GeneCards

GC05M043526.

HGNC

HGNC:16945. PAIP1.

MIM

605184. gene.

neXtProt

NX_Q9H074.

PharmGKB

PA134941557.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG253454.

HOGENOM

HOG000285987.

HOVERGEN

HBG053492.

InParanoid

Q9H074.

KO

K14322.

OMA

KMDMEEI.

OrthoDB

EOG4H72BQ.

PhylomeDB

Q9H074.

Enzyme and pathway databases

Reactome

REACT_21257. Metabolism of RNA.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

Q9H074.

Bgee

Q9H074.

CleanEx

HS_PAIP1.

Genevestigator

Q9H074.

GermOnline

ENSG00000172239. Homo sapiens.

Family and domain databases

Gene3D

1.25.40.180. 1 hit.

InterPro

IPR016024. ARM-type_fold.
IPR009818. Ataxin-2_C.
IPR016021. MIF4-like_typ_1/2/3.
IPR003890. MIF4G-like_typ-3.
[Graphical view]

Pfam

PF02854. MIF4G. 1 hit.
PF07145. PAM2. 1 hit.
[Graphical view]

SMART

SM00543. MIF4G. 1 hit.
[Graphical view]

SUPFAM

SSF48371. ARM-type_fold. 1 hit.

ProtoNet

Search...

Other

ChiTaRS

PAIP1. human.

EvolutionaryTrace

Q9H074.

GenomeRNAi

10605.

NextBio

40270.

SOURCE

Search...

Entry information

Entry name

PAIP1_HUMAN

Accession

Primary (citable) accession number: Q9H074
Secondary accession number(s): O60455, Q96B61, Q9BS63

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.