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Protein

Polyadenylate-binding protein-interacting protein 1

Gene

PAIP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.2 Publications

GO - Molecular functioni

  • RNA binding Source: ProtInc
  • translation activator activity Source: UniProtKB

GO - Biological processi

  • mRNA stabilization Source: UniProtKB
  • nuclear-transcribed mRNA poly(A) tail shortening Source: Reactome
  • translational initiation Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Translation regulation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000172239-MONOMER.
ReactomeiR-HSA-429947. Deadenylation of mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyadenylate-binding protein-interacting protein 1
Short name:
PABP-interacting protein 1
Short name:
PAIP-1
Short name:
Poly(A)-binding protein-interacting protein 1
Gene namesi
Name:PAIP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:16945. PAIP1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: ProtInc
  • cytosol Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000172239.
PharmGKBiPA134941557.

Polymorphism and mutation databases

BioMutaiPAIP1.
DMDMi46397025.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000581771 – 479Polyadenylate-binding protein-interacting protein 1Add BLAST479
Isoform 3 (identifier: Q9H074-3)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei21Omega-N-methylarginineCombined sources1
Isoform 3 (identifier: Q9H074-3)
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

EPDiQ9H074.
MaxQBiQ9H074.
PaxDbiQ9H074.
PeptideAtlasiQ9H074.
PRIDEiQ9H074.

PTM databases

iPTMnetiQ9H074.
PhosphoSitePlusiQ9H074.
SwissPalmiQ9H074.

Expressioni

Gene expression databases

BgeeiENSG00000172239.
CleanExiHS_PAIP1.
ExpressionAtlasiQ9H074. baseline and differential.
GenevisibleiQ9H074. HS.

Organism-specific databases

HPAiHPA073653.

Interactioni

Subunit structurei

Interacts with the RRM1-RRM2 and C-terminus regions of PABPC1 in a 1:1 stoichiometry. Interacts with EIF4A.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PABPC1P1194012EBI-81519,EBI-81531

Protein-protein interaction databases

BioGridi115851. 44 interactors.
IntActiQ9H074. 14 interactors.
MINTiMINT-196116.
STRINGi9606.ENSP00000302768.

Structurei

Secondary structure

1479
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi158 – 171Combined sources14
Helixi173 – 175Combined sources3
Helixi176 – 190Combined sources15
Helixi194 – 209Combined sources16
Helixi215 – 228Combined sources14
Helixi238 – 250Combined sources13
Helixi253 – 256Combined sources4
Helixi261 – 280Combined sources20
Beta strandi288 – 291Combined sources4
Helixi294 – 309Combined sources16
Helixi313 – 336Combined sources24
Helixi341 – 355Combined sources15
Helixi360 – 372Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JH4NMR-B123-144[»]
3NTWX-ray2.60B/D123-144[»]
3RK6X-ray2.00A/B157-373[»]
ProteinModelPortaliQ9H074.
SMRiQ9H074.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9H074.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini159 – 376MIF4GAdd BLAST218

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 143PABPC1-interacting motif-2 (PAM2)Add BLAST28
Regioni440 – 479PABPC1-interacting motif-1 (PAM1)Add BLAST40

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi10 – 36Gly-richAdd BLAST27
Compositional biasi45 – 98Pro-richAdd BLAST54

Domaini

Only the PABPC1-interacting motif-1 (PAM1) stimulates translation initiation.

Sequence similaritiesi

Contains 1 MIF4G domain.Curated

Phylogenomic databases

eggNOGiENOG410IPIA. Eukaryota.
ENOG41109D3. LUCA.
GeneTreeiENSGT00630000089897.
HOGENOMiHOG000285987.
HOVERGENiHBG053492.
InParanoidiQ9H074.
KOiK14322.
OMAiEPTFYTE.
OrthoDBiEOG091G05G0.
PhylomeDBiQ9H074.
TreeFamiTF325625.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR009818. Ataxin-2_C.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF02854. MIF4G. 1 hit.
PF07145. PAM2. 1 hit.
[Graphical view]
SMARTiSM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9H074-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDGFDRAPG AGRGRSRGLG RGGGGPEGGG FPNGAGPAER ARHQPPQPKA
60 70 80 90 100
PGFLQPPPLR QPRTTPPPGA QCEVPASPQR PSRPGALPEQ TRPLRAPPSS
110 120 130 140 150
QDKIPQQNSE SAMAKPQVVV APVLMSKLSV NAPEFYPSGY SSSYTESYED
160 170 180 190 200
GCEDYPTLSE YVQDFLNHLT EQPGSFETEI EQFAETLNGC VTTDDALQEL
210 220 230 240 250
VELIYQQATS IPNFSYMGAR LCNYLSHHLT ISPQSGNFRQ LLLQRCRTEY
260 270 280 290 300
EVKDQAAKGD EVTRKRFHAF VLFLGELYLN LEIKGTNGQV TRADILQVGL
310 320 330 340 350
RELLNALFSN PMDDNLICAV KLLKLTGSVL EDAWKEKGKM DMEEIIQRIE
360 370 380 390 400
NVVLDANCSR DVKQMLLKLV ELRSSNWGRV HATSTYREAT PENDPNYFMN
410 420 430 440 450
EPTFYTSDGV PFTAADPDYQ EKYQELLERE DFFPDYEENG TDLSGAGDPY
460 470
LDDIDDEMDP EIEEAYEKFC LESERKRKQ
Length:479
Mass (Da):53,525
Last modified:March 1, 2001 - v1
Checksum:iA4820607190A3A43
GO
Isoform 2 (identifier: Q9H074-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     10-88: Missing.

Show »
Length:400
Mass (Da):45,631
Checksum:i9743587A6F54BA25
GO
Isoform 3 (identifier: Q9H074-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-112: Missing.

Note: No experimental confirmation available.Combined sources
Show »
Length:367
Mass (Da):42,007
Checksum:iF03BA773A062FA9B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti239R → C in AAH15937 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0475031 – 112Missing in isoform 3. 1 PublicationAdd BLAST112
Alternative sequenceiVSP_01000510 – 88Missing in isoform 2. 1 PublicationAdd BLAST79

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013758 mRNA. Translation: AAC39697.2.
AL136920 mRNA. Translation: CAB66854.1.
AC114956 Genomic DNA. No translation available.
BC005295 mRNA. Translation: AAH05295.1.
BC015937 mRNA. Translation: AAH15937.1.
DB089732 mRNA. No translation available.
CCDSiCCDS3947.1. [Q9H074-1]
CCDS3948.1. [Q9H074-3]
CCDS47204.1. [Q9H074-2]
RefSeqiNP_006442.2. NM_006451.4. [Q9H074-1]
NP_877590.1. NM_182789.3. [Q9H074-2]
NP_899152.1. NM_183323.2. [Q9H074-3]
XP_005248287.1. XM_005248230.3. [Q9H074-3]
XP_016864445.1. XM_017008956.1. [Q9H074-3]
UniGeneiHs.482038.

Genome annotation databases

EnsembliENST00000306846; ENSP00000302768; ENSG00000172239. [Q9H074-1]
ENST00000338972; ENSP00000339622; ENSG00000172239. [Q9H074-3]
ENST00000436644; ENSP00000387729; ENSG00000172239. [Q9H074-2]
GeneIDi10605.
KEGGihsa:10605.
UCSCiuc003joa.4. human. [Q9H074-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013758 mRNA. Translation: AAC39697.2.
AL136920 mRNA. Translation: CAB66854.1.
AC114956 Genomic DNA. No translation available.
BC005295 mRNA. Translation: AAH05295.1.
BC015937 mRNA. Translation: AAH15937.1.
DB089732 mRNA. No translation available.
CCDSiCCDS3947.1. [Q9H074-1]
CCDS3948.1. [Q9H074-3]
CCDS47204.1. [Q9H074-2]
RefSeqiNP_006442.2. NM_006451.4. [Q9H074-1]
NP_877590.1. NM_182789.3. [Q9H074-2]
NP_899152.1. NM_183323.2. [Q9H074-3]
XP_005248287.1. XM_005248230.3. [Q9H074-3]
XP_016864445.1. XM_017008956.1. [Q9H074-3]
UniGeneiHs.482038.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JH4NMR-B123-144[»]
3NTWX-ray2.60B/D123-144[»]
3RK6X-ray2.00A/B157-373[»]
ProteinModelPortaliQ9H074.
SMRiQ9H074.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115851. 44 interactors.
IntActiQ9H074. 14 interactors.
MINTiMINT-196116.
STRINGi9606.ENSP00000302768.

PTM databases

iPTMnetiQ9H074.
PhosphoSitePlusiQ9H074.
SwissPalmiQ9H074.

Polymorphism and mutation databases

BioMutaiPAIP1.
DMDMi46397025.

Proteomic databases

EPDiQ9H074.
MaxQBiQ9H074.
PaxDbiQ9H074.
PeptideAtlasiQ9H074.
PRIDEiQ9H074.

Protocols and materials databases

DNASUi10605.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000306846; ENSP00000302768; ENSG00000172239. [Q9H074-1]
ENST00000338972; ENSP00000339622; ENSG00000172239. [Q9H074-3]
ENST00000436644; ENSP00000387729; ENSG00000172239. [Q9H074-2]
GeneIDi10605.
KEGGihsa:10605.
UCSCiuc003joa.4. human. [Q9H074-1]

Organism-specific databases

CTDi10605.
GeneCardsiPAIP1.
HGNCiHGNC:16945. PAIP1.
HPAiHPA073653.
MIMi605184. gene.
neXtProtiNX_Q9H074.
OpenTargetsiENSG00000172239.
PharmGKBiPA134941557.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IPIA. Eukaryota.
ENOG41109D3. LUCA.
GeneTreeiENSGT00630000089897.
HOGENOMiHOG000285987.
HOVERGENiHBG053492.
InParanoidiQ9H074.
KOiK14322.
OMAiEPTFYTE.
OrthoDBiEOG091G05G0.
PhylomeDBiQ9H074.
TreeFamiTF325625.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000172239-MONOMER.
ReactomeiR-HSA-429947. Deadenylation of mRNA.

Miscellaneous databases

ChiTaRSiPAIP1. human.
EvolutionaryTraceiQ9H074.
GeneWikiiPAIP1.
GenomeRNAii10605.
PROiQ9H074.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000172239.
CleanExiHS_PAIP1.
ExpressionAtlasiQ9H074. baseline and differential.
GenevisibleiQ9H074. HS.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR009818. Ataxin-2_C.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF02854. MIF4G. 1 hit.
PF07145. PAM2. 1 hit.
[Graphical view]
SMARTiSM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPAIP1_HUMAN
AccessioniPrimary (citable) accession number: Q9H074
Secondary accession number(s): A6NKV8
, O60455, Q96B61, Q9BS63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.