Q9H009 (NACA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nascent polypeptide-associated complex subunit alpha-2

Alternative name(s):
Alpha-NAC-like
Hom s 2.01
Nascent polypeptide-associated complex subunit alpha-like
Short name=NAC-alpha-like

Gene names

Name:	NACA2
Synonyms:	NACAL

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites) By similarity.
Subunit structure	Part of the nascent polypeptide-associated complex (NAC), consisting of NACA and BTF3. NAC associates with ribosomes through the BTF3 subunit. Both subunits can contact nascent polypeptide chains By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Expressed specifically in testis and skeletal muscle. Ref.3
Miscellaneous	NACAL arose from a recent (40-63 million year old), anthropoid primate-specific retroduplication of NACA.
Sequence similarities	Belongs to the NAC-alpha family. Contains 1 NAC-A/B (NAC-alpha/beta) domain. Contains 1 UBA domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transcription coactivator activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	Nascent polypeptide-associated complex subunit alpha-2		PRO_0000280746
Regions
	Domain	70 – 135	66	NAC-A/B
	Domain	176 – 213	38	UBA
Amino acid modifications
	Modified residue	214	1	Phosphothreonine Ref.4
Natural variations
	Natural variant	64	1	V → I. Corresponds to variant rs17531723 [ dbSNP | Ensembl ].		VAR_050218

Sequences

	Sequence		Length	Mass (Da)
	Q9H009 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C6B96A8370E9B24E	FASTA	215	23,223
	10 20 30 40 50 60 MPGEATETVP ATEQELPQSQ AETGSGTASD SGESVPGIEE QDSTQTTTQK AWLVAAAEID 70 80 90 100 110 120 EEPVGKAKQS RSEKRARKAM SKLGLLQVTG VTRVTIWKSK NILFVITKLD VYKSPASDAY 130 140 150 160 170 180 IVFGEAKIQD LSQQAQLAAA EKFRVQGEAV GNIQENTQTP TVQEESEEEE VDETGVEVKD 190 200 210 VKLVMSQANV SRAKAVRALK NNSNDIVNAI MELTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a novel isoform of alpha-nascent polypeptide-associated complex as IgE-defined autoantigen." Mossabeb R., Seiberler S., Mittermann I., Reininger R., Spitzauer S., Natter S., Verdino P., Keller W., Kraft D., Valenta R. J. Invest. Dermatol. 119:820-829(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Epithelium.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"Emergence of young human genes after a burst of retroposition in primates." Marques A.C., Dupanloup I., Vinckenbosch N., Reymond A., Kaessmann H. PLoS Biol. 3:E357-E357(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-210, TISSUE SPECIFICITY.
[4]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-214, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ278883 Genomic DNA. Translation: CAC06614.1. BC062710 mRNA. Translation: AAH62710.1. DQ120612 Genomic DNA. Translation: ABB92399.1.
IPI	IPI00007471.
RefSeq	NP_954984.1. NM_199290.3.
UniGene	Hs.591178.
3D structure databases
ProteinModelPortal	Q9H009.
ModBase	Search...
Protein family/group databases
Allergome	412. Hom s 2.
PTM databases
PhosphoSite	Q9H009.
Polymorphism databases
DMDM	74733511.
Proteomic databases
PaxDb	Q9H009.
PeptideAtlas	Q9H009.
PRIDE	Q9H009.
Protocols and materials databases
DNASU	342538.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000521764; ENSP00000427802; ENSG00000253506.
GeneID	342538.
KEGG	hsa:342538.
UCSC	uc002izj.2. human.
Organism-specific databases
CTD	342538.
GeneCards	GC17M059668.
HGNC	HGNC:23290. NACA2.
MIM	609274. gene.
neXtProt	NX_Q9H009.
PharmGKB	PA162396699.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1308.
HOGENOM	HOG000239674.
HOVERGEN	HBG082004.
InParanoid	Q9H009.
OMA	ASDAYIV.
PhylomeDB	Q9H009.
Gene expression databases
Bgee	Q9H009.
CleanEx	HS_NACA2.
Genevestigator	Q9H009.
Family and domain databases
InterPro	IPR016641. EGD2/NACA. IPR002715. Nas_poly-pep-assoc_cplx_dom. [Graphical view]
PANTHER	PTHR21713. PTHR21713. 1 hit.
Pfam	PF01849. NAC. 1 hit. [Graphical view]
PIRSF	PIRSF015901. NAC_alpha. 1 hit.
PROSITE	PS51151. NAC_AB. 1 hit. PS50030. UBA. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	342538.
NextBio	98309.
SOURCE	Search...

Entry information

Entry name

NACA2_HUMAN

Accession

Primary (citable) accession number: Q9H009
Secondary accession number(s): Q2VIR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.