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Q9H008

- LHPP_HUMAN

UniProt

Q9H008 - LHPP_HUMAN

Protein

Phospholysine phosphohistidine inorganic pyrophosphate phosphatase

Gene

LHPP

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Phosphatase that hydrolyzes imidodiphosphate, 3-phosphohistidine and 6-phospholysine. Has broad substrate specificity and can also hydrolyze inorganic diphosphate, but with lower efficiency By similarity.By similarity

    Catalytic activityi

    Diphosphate + H2O = 2 phosphate.1 Publication

    Cofactori

    Binds 1 magnesium ion per subunit.2 Publications

    pH dependencei

    Optimum pH is 7.0 for PNP, and 5.5 for PPi.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi17 – 171Magnesium
    Metal bindingi19 – 191Magnesium; via carbonyl oxygen
    Binding sitei189 – 1891Substrate
    Metal bindingi214 – 2141Magnesium

    GO - Molecular functioni

    1. inorganic diphosphatase activity Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. phosphohistidine phosphatase activity Source: UniProtKB
    4. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. phosphate-containing compound metabolic process Source: UniProtKB
    2. protein dephosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (EC:3.1.3.-, EC:3.6.1.1)
    Short name:
    hLHPP
    Gene namesi
    Name:LHPP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 10

    Organism-specific databases

    HGNCiHGNC:30042. LHPP.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. cytosol Source: UniProtKB
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA165548763.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 270270Phospholysine phosphohistidine inorganic pyrophosphate phosphatasePRO_0000305074Add
    BLAST

    Proteomic databases

    MaxQBiQ9H008.
    PaxDbiQ9H008.
    PRIDEiQ9H008.

    PTM databases

    PhosphoSiteiQ9H008.

    Expressioni

    Tissue specificityi

    Expressed in brain, and at lower levels in liver and kidney. Detected in thyroid (at protein level). Expressed in liver, kidney and moderately in brain.2 Publications

    Gene expression databases

    ArrayExpressiQ9H008.
    BgeeiQ9H008.
    GenevestigatoriQ9H008.

    Organism-specific databases

    HPAiHPA009163.
    HPA009269.

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Protein-protein interaction databases

    STRINGi9606.ENSP00000357835.

    Structurei

    Secondary structure

    1
    270
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 74
    Turni8 – 103
    Beta strandi13 – 164
    Turni19 – 213
    Beta strandi22 – 243
    Turni27 – 293
    Helixi36 – 4510
    Beta strandi46 – 538
    Helixi61 – 7010
    Helixi77 – 793
    Helixi83 – 9412
    Beta strandi98 – 1014
    Helixi104 – 1107
    Beta strandi119 – 1235
    Helixi127 – 1293
    Helixi132 – 14413
    Beta strandi150 – 1534
    Beta strandi157 – 1615
    Beta strandi164 – 1674
    Helixi169 – 18012
    Beta strandi185 – 1884
    Helixi192 – 20211
    Helixi206 – 2083
    Beta strandi209 – 2146
    Turni216 – 2194
    Helixi220 – 2256
    Beta strandi229 – 2357
    Helixi240 – 2445
    Beta strandi251 – 2566
    Helixi257 – 26711

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2X4DX-ray1.92A/B1-270[»]
    ProteinModelPortaliQ9H008.
    SMRiQ9H008. Positions 1-269.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni17 – 193Substrate binding
    Regioni54 – 552Substrate binding

    Sequence similaritiesi

    Belongs to the HAD-like hydrolase superfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0647.
    HOGENOMiHOG000068106.
    HOVERGENiHBG075146.
    InParanoidiQ9H008.
    KOiK11725.
    OMAiEAHQAVM.
    PhylomeDBiQ9H008.
    TreeFamiTF314344.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    3.40.50.10410. 1 hit.
    InterProiIPR023214. HAD-like_dom.
    IPR006357. HAD-SF_hydro_IIA.
    IPR006355. HAD-SF_hydro_IIA_hyp2.
    IPR023215. NPhePase-like_dom.
    [Graphical view]
    PfamiPF13344. Hydrolase_6. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01460. HAD-SF-IIA. 1 hit.
    TIGR01458. HAD-SF-IIA-hyp3. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9H008-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAPWGKRLAG VRGVLLDISG VLYDSGAGGG TAIAGSVEAV ARLKRSRLKV    50
    RFCTNESQKS RAELVGQLQR LGFDISEQEV TAPAPAACQI LKEQGLRPYL 100
    LIHDGVRSEF DQIDTSNPNC VVIADAGESF SYQNMNNAFQ VLMELEKPVL 150
    ISLGKGRYYK ETSGLMLDVG PYMKALEYAC GIKAEVVGKP SPEFFKSALQ 200
    AIGVEAHQAV MIGDDIVGDV GGAQRCGMRA LQVRTGKFRP SDEHHPEVKA 250
    DGYVDNLAEA VDLLLQHADK 270
    Length:270
    Mass (Da):29,165
    Last modified:October 2, 2007 - v2
    Checksum:i09C301584453E79D
    GO
    Isoform 2 (identifier: Q9H008-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         210-210: V → Q
         211-270: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:210
    Mass (Da):22,696
    Checksum:i93F0D37C2D348195
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti155 – 1551K → R in BAG51532. (PubMed:14702039)Curated
    Sequence conflicti170 – 1701G → C in AAI10345. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti94 – 941Q → R.3 Publications
    Corresponds to variant rs6597801 [ dbSNP | Ensembl ].
    VAR_035163

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei210 – 2101V → Q in isoform 2. 1 PublicationVSP_041685
    Alternative sequencei211 – 27060Missing in isoform 2. 1 PublicationVSP_041686Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB049629 mRNA. Translation: BAB16411.1.
    AK055532 mRNA. Translation: BAG51532.1.
    AL513190, AL445237 Genomic DNA. Translation: CAH70377.1.
    AL513190, AL391708, AL445237 Genomic DNA. Translation: CAH70378.1.
    AL391708, AL445237, AL513190 Genomic DNA. Translation: CAH74011.1.
    AL445237, AL513190 Genomic DNA. Translation: CAI17295.1.
    AL445237, AL391708, AL513190 Genomic DNA. Translation: CAI17296.1.
    BC110344 mRNA. Translation: AAI10345.1.
    BC113629 mRNA. Translation: AAI13630.1.
    BC113631 mRNA. Translation: AAI13632.1.
    CCDSiCCDS53587.1. [Q9H008-2]
    CCDS7640.1. [Q9H008-1]
    RefSeqiNP_001161352.1. NM_001167880.1. [Q9H008-2]
    NP_071409.3. NM_022126.3. [Q9H008-1]
    UniGeneiHs.527748.

    Genome annotation databases

    EnsembliENST00000368839; ENSP00000357832; ENSG00000107902. [Q9H008-2]
    ENST00000368842; ENSP00000357835; ENSG00000107902. [Q9H008-1]
    GeneIDi64077.
    KEGGihsa:64077.
    UCSCiuc001lhs.2. human. [Q9H008-1]
    uc001lht.2. human. [Q9H008-2]

    Polymorphism databases

    DMDMi158705883.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB049629 mRNA. Translation: BAB16411.1 .
    AK055532 mRNA. Translation: BAG51532.1 .
    AL513190 , AL445237 Genomic DNA. Translation: CAH70377.1 .
    AL513190 , AL391708 , AL445237 Genomic DNA. Translation: CAH70378.1 .
    AL391708 , AL445237 , AL513190 Genomic DNA. Translation: CAH74011.1 .
    AL445237 , AL513190 Genomic DNA. Translation: CAI17295.1 .
    AL445237 , AL391708 , AL513190 Genomic DNA. Translation: CAI17296.1 .
    BC110344 mRNA. Translation: AAI10345.1 .
    BC113629 mRNA. Translation: AAI13630.1 .
    BC113631 mRNA. Translation: AAI13632.1 .
    CCDSi CCDS53587.1. [Q9H008-2 ]
    CCDS7640.1. [Q9H008-1 ]
    RefSeqi NP_001161352.1. NM_001167880.1. [Q9H008-2 ]
    NP_071409.3. NM_022126.3. [Q9H008-1 ]
    UniGenei Hs.527748.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2X4D X-ray 1.92 A/B 1-270 [» ]
    ProteinModelPortali Q9H008.
    SMRi Q9H008. Positions 1-269.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000357835.

    PTM databases

    PhosphoSitei Q9H008.

    Polymorphism databases

    DMDMi 158705883.

    Proteomic databases

    MaxQBi Q9H008.
    PaxDbi Q9H008.
    PRIDEi Q9H008.

    Protocols and materials databases

    DNASUi 64077.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000368839 ; ENSP00000357832 ; ENSG00000107902 . [Q9H008-2 ]
    ENST00000368842 ; ENSP00000357835 ; ENSG00000107902 . [Q9H008-1 ]
    GeneIDi 64077.
    KEGGi hsa:64077.
    UCSCi uc001lhs.2. human. [Q9H008-1 ]
    uc001lht.2. human. [Q9H008-2 ]

    Organism-specific databases

    CTDi 64077.
    GeneCardsi GC10P126140.
    HGNCi HGNC:30042. LHPP.
    HPAi HPA009163.
    HPA009269.
    neXtProti NX_Q9H008.
    PharmGKBi PA165548763.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0647.
    HOGENOMi HOG000068106.
    HOVERGENi HBG075146.
    InParanoidi Q9H008.
    KOi K11725.
    OMAi EAHQAVM.
    PhylomeDBi Q9H008.
    TreeFami TF314344.

    Miscellaneous databases

    GenomeRNAii 64077.
    NextBioi 65859.
    PROi Q9H008.

    Gene expression databases

    ArrayExpressi Q9H008.
    Bgeei Q9H008.
    Genevestigatori Q9H008.

    Family and domain databases

    Gene3Di 3.40.50.1000. 2 hits.
    3.40.50.10410. 1 hit.
    InterProi IPR023214. HAD-like_dom.
    IPR006357. HAD-SF_hydro_IIA.
    IPR006355. HAD-SF_hydro_IIA_hyp2.
    IPR023215. NPhePase-like_dom.
    [Graphical view ]
    Pfami PF13344. Hydrolase_6. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR01460. HAD-SF-IIA. 1 hit.
    TIGR01458. HAD-SF-IIA-hyp3. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a cDNA for the human phospholysine phosphohistidine inorganic pyrophosphate phosphatase."
      Yokoi F., Hiraishi H., Izuhara K.
      J. Biochem. 133:607-614(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBUNIT, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, VARIANT ARG-94.
      Tissue: Cervix carcinoma.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-94.
      Tissue: Heart.
    3. "The DNA sequence and comparative analysis of human chromosome 10."
      Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
      , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
      Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ARG-94.
      Tissue: Brain.
    5. "Expression of new human inorganic pyrophosphatase in thyroid diseases: its intimate association with hyperthyroidism."
      Koike E., Toda S., Yokoi F., Izuhara K., Koike N., Itoh K., Miyazaki K., Sugihara H.
      Biochem. Biophys. Res. Commun. 341:691-696(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    6. "Crystal structure of human phospholysine phosphohistidine inorganic pyrophosphate phosphatase LHPP."
      Structural genomics consortium (SGC)
      Submitted (MAR-2010) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG AND MAGNESIUM IONS, COFACTOR.

    Entry informationi

    Entry nameiLHPP_HUMAN
    AccessioniPrimary (citable) accession number: Q9H008
    Secondary accession number(s): B3KP20
    , Q2TBE9, Q5VUV9, Q5VUW0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 10
      Human chromosome 10: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3