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Q9H008 (LHPP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phospholysine phosphohistidine inorganic pyrophosphate phosphatase

Short name=hLHPP
EC=3.1.3.-
EC=3.6.1.1
Gene names
Name:LHPP
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphatase that hydrolyzes imidodiphosphate, 3-phosphohistidine and 6-phospholysine. Has broad substrate specificity and can also hydrolyze inorganic diphosphate, but with lower efficiency By similarity.

Catalytic activity

Diphosphate + H2O = 2 phosphate. Ref.1

Cofactor

Binds 1 magnesium ion per subunit. Ref.1 Ref.6

Subunit structure

Homodimer. Ref.1

Subcellular location

Cytoplasm. Nucleus Ref.5.

Tissue specificity

Expressed in brain, and at lower levels in liver and kidney. Detected in thyroid (at protein level). Expressed in liver, kidney and moderately in brain. Ref.1 Ref.5

Sequence similarities

Belongs to the HAD-like hydrolase superfamily.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.0 for PNP, and 5.5 for PPi. Ref.1

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9H008-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9H008-2)

The sequence of this isoform differs from the canonical sequence as follows:
     210-210: V → Q
     211-270: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 270270Phospholysine phosphohistidine inorganic pyrophosphate phosphatase
PRO_0000305074

Regions

Region17 – 193Substrate binding
Region54 – 552Substrate binding

Sites

Metal binding171Magnesium
Metal binding191Magnesium; via carbonyl oxygen
Metal binding2141Magnesium
Binding site1891Substrate

Natural variations

Alternative sequence2101V → Q in isoform 2.
VSP_041685
Alternative sequence211 – 27060Missing in isoform 2.
VSP_041686
Natural variant941Q → R. Ref.1 Ref.2 Ref.4
Corresponds to variant rs6597801 [ dbSNP | Ensembl ].
VAR_035163

Experimental info

Sequence conflict1551K → R in BAG51532. Ref.2
Sequence conflict1701G → C in AAI10345. Ref.4

Secondary structure

....................................................... 270
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 2, 2007. Version 2.
Checksum: 09C301584453E79D

FASTA27029,165
        10         20         30         40         50         60 
MAPWGKRLAG VRGVLLDISG VLYDSGAGGG TAIAGSVEAV ARLKRSRLKV RFCTNESQKS 

        70         80         90        100        110        120 
RAELVGQLQR LGFDISEQEV TAPAPAACQI LKEQGLRPYL LIHDGVRSEF DQIDTSNPNC 

       130        140        150        160        170        180 
VVIADAGESF SYQNMNNAFQ VLMELEKPVL ISLGKGRYYK ETSGLMLDVG PYMKALEYAC 

       190        200        210        220        230        240 
GIKAEVVGKP SPEFFKSALQ AIGVEAHQAV MIGDDIVGDV GGAQRCGMRA LQVRTGKFRP 

       250        260        270 
SDEHHPEVKA DGYVDNLAEA VDLLLQHADK 

« Hide

Isoform 2 [UniParc].

Checksum: 93F0D37C2D348195
Show »

FASTA21022,696

References

« Hide 'large scale' references
[1]"Molecular cloning of a cDNA for the human phospholysine phosphohistidine inorganic pyrophosphate phosphatase."
Yokoi F., Hiraishi H., Izuhara K.
J. Biochem. 133:607-614(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBUNIT, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, VARIANT ARG-94.
Tissue: Cervix carcinoma.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-94.
Tissue: Heart.
[3]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ARG-94.
Tissue: Brain.
[5]"Expression of new human inorganic pyrophosphatase in thyroid diseases: its intimate association with hyperthyroidism."
Koike E., Toda S., Yokoi F., Izuhara K., Koike N., Itoh K., Miyazaki K., Sugihara H.
Biochem. Biophys. Res. Commun. 341:691-696(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]"Crystal structure of human phospholysine phosphohistidine inorganic pyrophosphate phosphatase LHPP."
Structural genomics consortium (SGC)
Submitted (MAR-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG AND MAGNESIUM IONS, COFACTOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB049629 mRNA. Translation: BAB16411.1.
AK055532 mRNA. Translation: BAG51532.1.
AL513190, AL445237 Genomic DNA. Translation: CAH70377.1.
AL513190, AL391708, AL445237 Genomic DNA. Translation: CAH70378.1.
AL391708, AL445237, AL513190 Genomic DNA. Translation: CAH74011.1.
AL445237, AL513190 Genomic DNA. Translation: CAI17295.1.
AL445237, AL391708, AL513190 Genomic DNA. Translation: CAI17296.1.
BC110344 mRNA. Translation: AAI10345.1.
BC113629 mRNA. Translation: AAI13630.1.
BC113631 mRNA. Translation: AAI13632.1.
RefSeqNP_001161352.1. NM_001167880.1.
NP_071409.3. NM_022126.3.
UniGeneHs.527748.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2X4DX-ray1.92A/B1-270[»]
ProteinModelPortalQ9H008.
SMRQ9H008. Positions 1-269.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000357835.

PTM databases

PhosphoSiteQ9H008.

Polymorphism databases

DMDM158705883.

Proteomic databases

PaxDbQ9H008.
PRIDEQ9H008.

Protocols and materials databases

DNASU64077.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000368839; ENSP00000357832; ENSG00000107902. [Q9H008-2]
ENST00000368842; ENSP00000357835; ENSG00000107902. [Q9H008-1]
GeneID64077.
KEGGhsa:64077.
UCSCuc001lhs.2. human. [Q9H008-1]
uc001lht.2. human. [Q9H008-2]

Organism-specific databases

CTD64077.
GeneCardsGC10P126140.
HGNCHGNC:30042. LHPP.
HPAHPA009163.
HPA009269.
neXtProtNX_Q9H008.
PharmGKBPA165548763.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0647.
HOGENOMHOG000068106.
HOVERGENHBG075146.
InParanoidQ9H008.
KOK11725.
OMAQAMGVEA.
PhylomeDBQ9H008.
TreeFamTF314344.

Gene expression databases

ArrayExpressQ9H008.
BgeeQ9H008.
GenevestigatorQ9H008.

Family and domain databases

Gene3D3.40.50.1000. 2 hits.
3.40.50.10410. 1 hit.
InterProIPR023214. HAD-like_dom.
IPR006357. HAD-SF_hydro_IIA.
IPR006355. HAD-SF_hydro_IIA_hyp2.
IPR023215. NPhePase-like_dom.
[Graphical view]
PfamPF13344. Hydrolase_6. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR01460. HAD-SF-IIA. 1 hit.
TIGR01458. HAD-SF-IIA-hyp3. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi64077.
NextBio65859.
PROQ9H008.

Entry information

Entry nameLHPP_HUMAN
AccessionPrimary (citable) accession number: Q9H008
Secondary accession number(s): B3KP20 expand/collapse secondary AC list , Q2TBE9, Q5VUV9, Q5VUW0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: April 16, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM