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Q9H000

- MKRN2_HUMAN

UniProt

Q9H000 - MKRN2_HUMAN

Protein

Probable E3 ubiquitin-protein ligase makorin-2

Gene

MKRN2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (15 Mar 2004)
      Previous versions | rss
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    Functioni

    E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri2 – 2928C3H1-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri31 – 5828C3H1-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri165 – 19228C3H1-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri238 – 29255RING-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri321 – 35030C3H1-type 4PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. poly(A) RNA binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable E3 ubiquitin-protein ligase makorin-2 (EC:6.3.2.-)
    Alternative name(s):
    RING finger protein 62
    Gene namesi
    Name:MKRN2
    Synonyms:RNF62
    ORF Names:HSPC070
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:7113. MKRN2.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: UniProtKB

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30832.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 416416Probable E3 ubiquitin-protein ligase makorin-2PRO_0000055955Add
    BLAST

    Proteomic databases

    MaxQBiQ9H000.
    PaxDbiQ9H000.
    PRIDEiQ9H000.

    PTM databases

    PhosphoSiteiQ9H000.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    ArrayExpressiQ9H000.
    BgeeiQ9H000.
    CleanExiHS_MKRN2.
    GenevestigatoriQ9H000.

    Organism-specific databases

    HPAiHPA037559.
    HPA037560.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HTTP428583EBI-2341005,EBI-466029

    Protein-protein interaction databases

    BioGridi117142. 3 interactions.
    IntActiQ9H000. 3 interactions.
    STRINGi9606.ENSP00000170447.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9H000.
    SMRiQ9H000. Positions 233-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni193 – 22230Makorin-type Cys-HisAdd
    BLAST

    Sequence similaritiesi

    Contains 4 C3H1-type zinc fingers.PROSITE-ProRule annotation
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri2 – 2928C3H1-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri31 – 5828C3H1-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri165 – 19228C3H1-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri238 – 29255RING-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri321 – 35030C3H1-type 4PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5084.
    HOGENOMiHOG000252976.
    HOVERGENiHBG066965.
    InParanoidiQ9H000.
    KOiK15687.
    OMAiYCAYGTR.
    OrthoDBiEOG79W95Q.
    PhylomeDBiQ9H000.
    TreeFamiTF315108.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR026293. Makorin_2.
    IPR018957. Znf_C3HC4_RING-type.
    IPR000571. Znf_CCCH.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view]
    PANTHERiPTHR11224:SF17. PTHR11224:SF17. 1 hit.
    PfamiPF00097. zf-C3HC4. 1 hit.
    PF00642. zf-CCCH. 2 hits.
    [Graphical view]
    SMARTiSM00184. RING. 1 hit.
    SM00356. ZnF_C3H1. 4 hits.
    [Graphical view]
    PROSITEiPS50103. ZF_C3H1. 4 hits.
    PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9H000-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSTKQITCRY FMHGVCREGS QCLFSHDLAN SKPSTICKYY QKGYCAYGTR    50
    CRYDHTRPSA AAGGAVGTMA HSVPSPAFHS PHPPSEVTAS IVKTNSHEPG 100
    KREKRTLVLR DRNLSGMAER KTQPSMVSNP GSCSDPQPSP EMKPHSYLDA 150
    IRSGLDDVEA SSSYSNEQQL CPYAAAGECR FGDACVYLHG EVCEICRLQV 200
    LHPFDPEQRK AHEKICMLTF EHEMEKAFAF QASQDKVCSI CMEVILEKAS 250
    ASERRFGILS NCNHTYCLSC IRQWRCAKQF ENPIIKSCPE CRVISEFVIP 300
    SVYWVEDQNK KNELIEAFKQ GMGKKACKYF EQGKGTCPFG SKCLYRHAYP 350
    DGRLAEPEKP RKQLSSQGTV RFFNSVRLWD FIENRESRHV PNNEDVDMTE 400
    LGDLFMHLSG VESSEP 416
    Length:416
    Mass (Da):46,940
    Last modified:March 15, 2004 - v2
    Checksum:i1F1564E589EB6632
    GO
    Isoform 2 (identifier: Q9H000-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         8-50: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:373
    Mass (Da):41,985
    Checksum:i21F2B0DABBF9467D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti186 – 1861V → F in AAG30426. (PubMed:11597136)Curated
    Sequence conflicti186 – 1861V → F in AAG27595. (PubMed:11597136)Curated
    Sequence conflicti278 – 2781K → E in AAF29042. (PubMed:11042152)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti388 – 3881R → Q.
    Corresponds to variant rs5746260 [ dbSNP | Ensembl ].
    VAR_052085

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei8 – 5043Missing in isoform 2. 1 PublicationVSP_055275Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302084 mRNA. Translation: AAG30426.1.
    AF277170
    , AF277164, AF277165, AF277166, AF277167, AF277168, AF277169 Genomic DNA. Translation: AAG27595.1.
    AF161555 mRNA. Translation: AAF29042.2.
    AK091318 mRNA. Translation: BAG52337.1.
    AK298463 mRNA. Translation: BAG60676.1.
    AC018500 Genomic DNA. No translation available.
    CH471055 Genomic DNA. Translation: EAW64132.1.
    CH471055 Genomic DNA. Translation: EAW64133.1.
    BC001799 mRNA. Translation: AAH01799.2.
    BC015715 mRNA. Translation: AAH15715.1.
    AL834512 mRNA. Translation: CAD39168.1.
    CCDSiCCDS33702.1. [Q9H000-1]
    CCDS63545.1. [Q9H000-2]
    RefSeqiNP_001258636.1. NM_001271707.1.
    NP_054879.3. NM_014160.4.
    UniGeneiHs.591666.

    Genome annotation databases

    EnsembliENST00000170447; ENSP00000170447; ENSG00000075975. [Q9H000-1]
    ENST00000411987; ENSP00000396340; ENSG00000075975. [Q9H000-2]
    GeneIDi23609.
    KEGGihsa:23609.
    UCSCiuc003bxd.4. human. [Q9H000-1]

    Polymorphism databases

    DMDMi45645205.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302084 mRNA. Translation: AAG30426.1 .
    AF277170
    , AF277164 , AF277165 , AF277166 , AF277167 , AF277168 , AF277169 Genomic DNA. Translation: AAG27595.1 .
    AF161555 mRNA. Translation: AAF29042.2 .
    AK091318 mRNA. Translation: BAG52337.1 .
    AK298463 mRNA. Translation: BAG60676.1 .
    AC018500 Genomic DNA. No translation available.
    CH471055 Genomic DNA. Translation: EAW64132.1 .
    CH471055 Genomic DNA. Translation: EAW64133.1 .
    BC001799 mRNA. Translation: AAH01799.2 .
    BC015715 mRNA. Translation: AAH15715.1 .
    AL834512 mRNA. Translation: CAD39168.1 .
    CCDSi CCDS33702.1. [Q9H000-1 ]
    CCDS63545.1. [Q9H000-2 ]
    RefSeqi NP_001258636.1. NM_001271707.1.
    NP_054879.3. NM_014160.4.
    UniGenei Hs.591666.

    3D structure databases

    ProteinModelPortali Q9H000.
    SMRi Q9H000. Positions 233-296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 117142. 3 interactions.
    IntActi Q9H000. 3 interactions.
    STRINGi 9606.ENSP00000170447.

    PTM databases

    PhosphoSitei Q9H000.

    Polymorphism databases

    DMDMi 45645205.

    Proteomic databases

    MaxQBi Q9H000.
    PaxDbi Q9H000.
    PRIDEi Q9H000.

    Protocols and materials databases

    DNASUi 23609.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000170447 ; ENSP00000170447 ; ENSG00000075975 . [Q9H000-1 ]
    ENST00000411987 ; ENSP00000396340 ; ENSG00000075975 . [Q9H000-2 ]
    GeneIDi 23609.
    KEGGi hsa:23609.
    UCSCi uc003bxd.4. human. [Q9H000-1 ]

    Organism-specific databases

    CTDi 23609.
    GeneCardsi GC03P012598.
    HGNCi HGNC:7113. MKRN2.
    HPAi HPA037559.
    HPA037560.
    MIMi 608426. gene.
    neXtProti NX_Q9H000.
    PharmGKBi PA30832.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5084.
    HOGENOMi HOG000252976.
    HOVERGENi HBG066965.
    InParanoidi Q9H000.
    KOi K15687.
    OMAi YCAYGTR.
    OrthoDBi EOG79W95Q.
    PhylomeDBi Q9H000.
    TreeFami TF315108.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    ChiTaRSi MKRN2. human.
    GenomeRNAii 23609.
    NextBioi 35473941.
    PROi Q9H000.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9H000.
    Bgeei Q9H000.
    CleanExi HS_MKRN2.
    Genevestigatori Q9H000.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR026293. Makorin_2.
    IPR018957. Znf_C3HC4_RING-type.
    IPR000571. Znf_CCCH.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view ]
    PANTHERi PTHR11224:SF17. PTHR11224:SF17. 1 hit.
    Pfami PF00097. zf-C3HC4. 1 hit.
    PF00642. zf-CCCH. 2 hits.
    [Graphical view ]
    SMARTi SM00184. RING. 1 hit.
    SM00356. ZnF_C3H1. 4 hits.
    [Graphical view ]
    PROSITEi PS50103. ZF_C3H1. 4 hits.
    PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Phylogenetic conservation of the makorin-2 gene, encoding a multiple zinc-finger protein, antisense to the raf1 proto-oncogene."
      Gray T.A., Azama K., Whitmore K., Min A., Abe S., Nicholls R.D.
      Genomics 77:119-126(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    2. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
      Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
      , Gu J., Chen S.-J., Chen Z.
      Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Umbilical cord blood.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain.
    4. "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
      , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
      Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Eye and Skin.
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 115-416 (ISOFORM 1/2).
      Tissue: Melanoma.
    8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMKRN2_HUMAN
    AccessioniPrimary (citable) accession number: Q9H000
    Secondary accession number(s): A6NIA2
    , B3KRC5, B4DPR4, Q8N391, Q96BD4, Q9BUY2, Q9NRY1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2001
    Last sequence update: March 15, 2004
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Partially overlaps and is antisense to the RAF1 proto-oncogene.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3