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Protein

Probable E3 ubiquitin-protein ligase makorin-2

Gene

MKRN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.By similarity

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri2 – 2928C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri31 – 5828C3H1-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri165 – 19228C3H1-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri238 – 29255RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri321 – 35030C3H1-type 4PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. poly(A) RNA binding Source: UniProtKB
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. protein ubiquitination Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase makorin-2 (EC:6.3.2.-)
Alternative name(s):
RING finger protein 62
Gene namesi
Name:MKRN2
Synonyms:RNF62
ORF Names:HSPC070
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:7113. MKRN2.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30832.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 416416Probable E3 ubiquitin-protein ligase makorin-2PRO_0000055955Add
BLAST

Proteomic databases

MaxQBiQ9H000.
PaxDbiQ9H000.
PRIDEiQ9H000.

PTM databases

PhosphoSiteiQ9H000.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiQ9H000.
CleanExiHS_MKRN2.
ExpressionAtlasiQ9H000. baseline and differential.
GenevestigatoriQ9H000.

Organism-specific databases

HPAiHPA037559.
HPA037560.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HTTP428583EBI-2341005,EBI-466029

Protein-protein interaction databases

BioGridi117142. 16 interactions.
IntActiQ9H000. 3 interactions.
STRINGi9606.ENSP00000170447.

Structurei

3D structure databases

ProteinModelPortaliQ9H000.
SMRiQ9H000. Positions 234-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni193 – 22230Makorin-type Cys-HisAdd
BLAST

Sequence similaritiesi

Contains 4 C3H1-type zinc fingers.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri2 – 2928C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri31 – 5828C3H1-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri165 – 19228C3H1-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri238 – 29255RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri321 – 35030C3H1-type 4PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5084.
GeneTreeiENSGT00390000014093.
HOGENOMiHOG000252976.
HOVERGENiHBG066965.
InParanoidiQ9H000.
KOiK15687.
OMAiYCAYGTR.
OrthoDBiEOG79W95Q.
PhylomeDBiQ9H000.
TreeFamiTF315108.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR026293. Makorin_2.
IPR018957. Znf_C3HC4_RING-type.
IPR000571. Znf_CCCH.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR11224:SF17. PTHR11224:SF17. 1 hit.
PfamiPF00097. zf-C3HC4. 1 hit.
PF00642. zf-CCCH. 2 hits.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00356. ZnF_C3H1. 4 hits.
[Graphical view]
PROSITEiPS50103. ZF_C3H1. 4 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9H000-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTKQITCRY FMHGVCREGS QCLFSHDLAN SKPSTICKYY QKGYCAYGTR
60 70 80 90 100
CRYDHTRPSA AAGGAVGTMA HSVPSPAFHS PHPPSEVTAS IVKTNSHEPG
110 120 130 140 150
KREKRTLVLR DRNLSGMAER KTQPSMVSNP GSCSDPQPSP EMKPHSYLDA
160 170 180 190 200
IRSGLDDVEA SSSYSNEQQL CPYAAAGECR FGDACVYLHG EVCEICRLQV
210 220 230 240 250
LHPFDPEQRK AHEKICMLTF EHEMEKAFAF QASQDKVCSI CMEVILEKAS
260 270 280 290 300
ASERRFGILS NCNHTYCLSC IRQWRCAKQF ENPIIKSCPE CRVISEFVIP
310 320 330 340 350
SVYWVEDQNK KNELIEAFKQ GMGKKACKYF EQGKGTCPFG SKCLYRHAYP
360 370 380 390 400
DGRLAEPEKP RKQLSSQGTV RFFNSVRLWD FIENRESRHV PNNEDVDMTE
410
LGDLFMHLSG VESSEP
Length:416
Mass (Da):46,940
Last modified:March 15, 2004 - v2
Checksum:i1F1564E589EB6632
GO
Isoform 2 (identifier: Q9H000-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     8-50: Missing.

Note: No experimental confirmation available.

Show »
Length:373
Mass (Da):41,985
Checksum:i21F2B0DABBF9467D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti186 – 1861V → F in AAG30426. (PubMed:11597136)Curated
Sequence conflicti186 – 1861V → F in AAG27595. (PubMed:11597136)Curated
Sequence conflicti278 – 2781K → E in AAF29042. (PubMed:11042152)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti388 – 3881R → Q.
Corresponds to variant rs5746260 [ dbSNP | Ensembl ].
VAR_052085

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei8 – 5043Missing in isoform 2. 1 PublicationVSP_055275Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302084 mRNA. Translation: AAG30426.1.
AF277170
, AF277164, AF277165, AF277166, AF277167, AF277168, AF277169 Genomic DNA. Translation: AAG27595.1.
AF161555 mRNA. Translation: AAF29042.2.
AK091318 mRNA. Translation: BAG52337.1.
AK298463 mRNA. Translation: BAG60676.1.
AC018500 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64132.1.
CH471055 Genomic DNA. Translation: EAW64133.1.
BC001799 mRNA. Translation: AAH01799.2.
BC015715 mRNA. Translation: AAH15715.1.
AL834512 mRNA. Translation: CAD39168.1.
CCDSiCCDS33702.1. [Q9H000-1]
CCDS63545.1. [Q9H000-2]
RefSeqiNP_001258636.1. NM_001271707.1. [Q9H000-2]
NP_054879.3. NM_014160.4. [Q9H000-1]
UniGeneiHs.591666.

Genome annotation databases

EnsembliENST00000170447; ENSP00000170447; ENSG00000075975. [Q9H000-1]
ENST00000411987; ENSP00000396340; ENSG00000075975. [Q9H000-2]
GeneIDi23609.
KEGGihsa:23609.
UCSCiuc003bxd.4. human. [Q9H000-1]
uc011aus.3. human.

Polymorphism databases

DMDMi45645205.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302084 mRNA. Translation: AAG30426.1.
AF277170
, AF277164, AF277165, AF277166, AF277167, AF277168, AF277169 Genomic DNA. Translation: AAG27595.1.
AF161555 mRNA. Translation: AAF29042.2.
AK091318 mRNA. Translation: BAG52337.1.
AK298463 mRNA. Translation: BAG60676.1.
AC018500 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64132.1.
CH471055 Genomic DNA. Translation: EAW64133.1.
BC001799 mRNA. Translation: AAH01799.2.
BC015715 mRNA. Translation: AAH15715.1.
AL834512 mRNA. Translation: CAD39168.1.
CCDSiCCDS33702.1. [Q9H000-1]
CCDS63545.1. [Q9H000-2]
RefSeqiNP_001258636.1. NM_001271707.1. [Q9H000-2]
NP_054879.3. NM_014160.4. [Q9H000-1]
UniGeneiHs.591666.

3D structure databases

ProteinModelPortaliQ9H000.
SMRiQ9H000. Positions 234-321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117142. 16 interactions.
IntActiQ9H000. 3 interactions.
STRINGi9606.ENSP00000170447.

PTM databases

PhosphoSiteiQ9H000.

Polymorphism databases

DMDMi45645205.

Proteomic databases

MaxQBiQ9H000.
PaxDbiQ9H000.
PRIDEiQ9H000.

Protocols and materials databases

DNASUi23609.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000170447; ENSP00000170447; ENSG00000075975. [Q9H000-1]
ENST00000411987; ENSP00000396340; ENSG00000075975. [Q9H000-2]
GeneIDi23609.
KEGGihsa:23609.
UCSCiuc003bxd.4. human. [Q9H000-1]
uc011aus.3. human.

Organism-specific databases

CTDi23609.
GeneCardsiGC03P012598.
HGNCiHGNC:7113. MKRN2.
HPAiHPA037559.
HPA037560.
MIMi608426. gene.
neXtProtiNX_Q9H000.
PharmGKBiPA30832.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5084.
GeneTreeiENSGT00390000014093.
HOGENOMiHOG000252976.
HOVERGENiHBG066965.
InParanoidiQ9H000.
KOiK15687.
OMAiYCAYGTR.
OrthoDBiEOG79W95Q.
PhylomeDBiQ9H000.
TreeFamiTF315108.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiMKRN2. human.
GenomeRNAii23609.
NextBioi35473941.
PROiQ9H000.
SOURCEiSearch...

Gene expression databases

BgeeiQ9H000.
CleanExiHS_MKRN2.
ExpressionAtlasiQ9H000. baseline and differential.
GenevestigatoriQ9H000.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR026293. Makorin_2.
IPR018957. Znf_C3HC4_RING-type.
IPR000571. Znf_CCCH.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR11224:SF17. PTHR11224:SF17. 1 hit.
PfamiPF00097. zf-C3HC4. 1 hit.
PF00642. zf-CCCH. 2 hits.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00356. ZnF_C3H1. 4 hits.
[Graphical view]
PROSITEiPS50103. ZF_C3H1. 4 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Phylogenetic conservation of the makorin-2 gene, encoding a multiple zinc-finger protein, antisense to the raf1 proto-oncogene."
    Gray T.A., Azama K., Whitmore K., Min A., Abe S., Nicholls R.D.
    Genomics 77:119-126(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  2. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Umbilical cord blood.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.
  4. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye and Skin.
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 115-416 (ISOFORM 1/2).
    Tissue: Melanoma.
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMKRN2_HUMAN
AccessioniPrimary (citable) accession number: Q9H000
Secondary accession number(s): A6NIA2
, B3KRC5, B4DPR4, Q8N391, Q96BD4, Q9BUY2, Q9NRY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: March 15, 2004
Last modified: February 4, 2015
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Partially overlaps and is antisense to the RAF1 proto-oncogene.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.