Q9GZZ6 (ACH10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuronal acetylcholine receptor subunit alpha-10

Alternative name(s):
Nicotinic acetylcholine receptor subunit alpha-10
Short name=NACHR alpha-10

Gene names

Name:	CHRNA10
Synonyms:	NACHRA10

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. Ref.1
Subunit structure	Forms heterooligomeric channels in conjunction with CHRNA9. The native outer hair cell receptor may be composed of CHRNA9-CHRNA10 heterooligomers.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein Probable. Cell membrane; Multi-pass membrane protein Probable.
Tissue specificity	Expressed in inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes. Ref.1 Ref.4
Miscellaneous	The heterooligomeric receptor composed of CHRNA9 and CHRNA10 has an atypical pharmacological profile, binding several non-nicotinic ligands including strychnine (a glycine receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist).
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-10/CHRNA10 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	Calcium channel Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	detection of mechanical stimulus involved in sensory perception of sound Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration Inferred from genetic interaction PubMed 18420419. Source: MGI inner ear morphogenesis Inferred from electronic annotation. Source: Compara regulation of cell proliferation Traceable author statement Ref.2. Source: UniProtKB synaptic transmission, cholinergic Traceable author statement Ref.2. Source: UniProtKB
Cellular_component	axon Inferred from electronic annotation. Source: Compara cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Traceable author statement Ref.2. Source: UniProtKB perikaryon Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetylcholine-activated cation-selective channel activity Inferred from electronic annotation. Source: Compara calcium channel activity Inferred from electronic annotation. Source: UniProtKB-KW receptor binding Traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 450	426	Neuronal acetylcholine receptor subunit alpha-10		PRO_0000000376
Regions
	Topological domain	25 – 237	213	Extracellular Potential
	Transmembrane	238 – 258	21	Helical; Potential
	Transmembrane	268 – 288	21	Helical; Potential
	Transmembrane	302 – 322	21	Helical; Potential
	Topological domain	323 – 428	106	Cytoplasmic Potential
	Transmembrane	429 – 449	21	Helical; Potential
Amino acid modifications
	Glycosylation	40	1	N-linked (GlcNAc...) Potential
	Glycosylation	56	1	N-linked (GlcNAc...) Potential
	Disulfide bond	154 ↔ 168		By similarity
	Disulfide bond	218 ↔ 219		Associated with receptor activation By similarity
Natural variations
	Natural variant	355	1	E → A. Corresponds to variant rs2231547 [ dbSNP | Ensembl ].		VAR_048172

Sequences

	Sequence		Length	Mass (Da)
	Q9GZZ6 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 0A1DAD32D9069870	FASTA	450	49,705
	10 20 30 40 50 60 MGLRSHHLSL GLLLLFLLPA ECLGAEGRLA LKLFRDLFAN YTSALRPVAD TDQTLNVTLE 70 80 90 100 110 120 VTLSQIIDMD ERNQVLTLYL WIRQEWTDAY LRWDPNAYGG LDAIRIPSSL VWRPDIVLYN 130 140 150 160 170 180 KADAQPPGSA STNVVLRHDG AVRWDAPAIT RSSCRVDVAA FPFDAQHCGL TFGSWTHGGH 190 200 210 220 230 240 QLDVRPRGAA ASLADFVENV EWRVLGMPAR RRVLTYGCCS EPYPDVTFTL LLRRRAAAYV 250 260 270 280 290 300 CNLLLPCVLI SLLAPLAFHL PADSGEKVSL GVTVLLALTV FQLLLAESMP PAESVPLIGK 310 320 330 340 350 360 YYMATMTMVT FSTALTILIM NLHYCGPSVR PVPAWARALL LGHLARGLCV RERGEPCGQS 370 380 390 400 410 420 RPPELSPSPQ SPEGGAGPPA GPCHEPRCLC RQEALLHHVA TIANTFRSHR AAQRCHEDWK 430 440 450 RLARVMDRFF LAIFFSMALV MSLLVLVQAL

References

[1]	"A novel human nicotinic receptor subunit, alpha10, that confers functionality to the alpha9-subunit." Sgard F., Charpantier E., Bertrand S., Walker N., Caput D., Graham D., Bertrand D., Besnard F. Mol. Pharmacol. 61:150-159(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CHRNA9, TISSUE SPECIFICITY. Tissue: Skeletal muscle.
[2]	"Molecular cloning and mapping of the human nicotinic acetylcholine receptor alpha10 (CHRNA10)." Lustig L.R., Peng H., Hiel H., Yamamoto T., Fuchs P.A. Genomics 73:272-283(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Inner ear and Tonsil.
[3]	"Identification and functional characterisation of a novel human neuronal nicotinic acetylcholine receptor subunit alpha 10." Vandenberk I., van der Helm L., Nieuwstraten D.M., Verfaille C., Kremer A., van der Spek P., Masure S., Hoefnagel E., Yon J., Groot Kormelink P.J., Luyten W.H., Grantham C.J. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Characterization of the human nicotinic acetylcholine receptor subunit alpha (alpha) 9 (CHRNA9) and alpha (alpha) 10 (CHRNA10) in lymphocytes." Peng H., Ferris R.L., Matthews T., Hiel H., Lopez-Albaitero A., Lustig L.R. Life Sci. 76:263-280(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ278118 mRNA. Translation: CAC20435.1. AF199235 mRNA. Translation: AAG00795.2. AF327367 Genomic DNA. Translation: AAK14333.1. AJ295237 mRNA. Translation: CAC16144.1.
IPI	IPI00026461.
RefSeq	NP_065135.2. NM_020402.2.
UniGene	Hs.732806.
3D structure databases
ProteinModelPortal	Q9GZZ6.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000250699.
Polymorphism databases
DMDM	14916526.
Proteomic databases
PaxDb	Q9GZZ6.
PRIDE	Q9GZZ6.
Protocols and materials databases
DNASU	57053.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000250699; ENSP00000250699; ENSG00000129749.
GeneID	57053.
KEGG	hsa:57053.
UCSC	uc001lyf.3. human.
Organism-specific databases
CTD	57053.
GeneCards	GC11M003687.
HGNC	HGNC:13800. CHRNA10.
MIM	606372. gene.
neXtProt	NX_Q9GZZ6.
PharmGKB	PA26488.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG306809.
HOGENOM	HOG000006756.
HOVERGEN	HBG003756.
InParanoid	Q9GZZ6.
KO	K04811.
OMA	DGAVRWD.
OrthoDB	EOG4S7JQ0.
PhylomeDB	Q9GZZ6.
Gene expression databases
ArrayExpress	Q9GZZ6.
Bgee	Q9GZZ6.
CleanEx	HS_CHRNA10.
Genevestigator	Q9GZZ6.
GermOnline	ENSG00000129749. Homo sapiens.
Family and domain databases
Gene3D	2.70.170.10. 1 hit.
InterPro	IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. IPR002394. Nicotinic_acetylcholine_rcpt. [Graphical view]
PANTHER	PTHR18945. PTHR18945. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00254. NICOTINICR. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q9GZZ6.
ChEMBL	CHEMBL2551.
DrugBank	DB01161. Chloroprocaine. DB00333. Methadone. DB00184. Nicotine. DB01090. Pentolinium. DB00721. Procaine. DB01116. Trimethaphan.
GenomeRNAi	57053.
NextBio	62824.
SOURCE	Search...

Entry information

Entry name

ACH10_HUMAN

Accession

Primary (citable) accession number: Q9GZZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.