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Q9GZZ6 (ACH10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuronal acetylcholine receptor subunit alpha-10
Alternative name(s):
Nicotinic acetylcholine receptor subunit alpha-10
Short name=NACHR alpha-10
Gene names
Name:CHRNA10
Synonyms:NACHRA10
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length450 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. Ref.1

Subunit structure

Forms heterooligomeric channels in conjunction with CHRNA9. The native outer hair cell receptor may be composed of CHRNA9-CHRNA10 heterooligomers.

Subcellular location

Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein Probable. Cell membrane; Multi-pass membrane protein Probable.

Tissue specificity

Expressed in inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes. Ref.1 Ref.4

Miscellaneous

The heterooligomeric receptor composed of CHRNA9 and CHRNA10 has an atypical pharmacological profile, binding several non-nicotinic ligands including strychnine (a glycine receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist).

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-10/CHRNA10 sub-subfamily. [View classification]

Ontologies

Keywords
   Biological processCalcium transport
Ion transport
Transport
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   Coding sequence diversityPolymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   LigandCalcium
   Molecular functionCalcium channel
Ion channel
Ligand-gated ion channel
Receptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdetection of mechanical stimulus involved in sensory perception of sound

Inferred from electronic annotation. Source: Ensembl

inner ear morphogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration

Inferred from genetic interaction PubMed 18420419. Source: MGI

regulation of cell proliferation

Traceable author statement Ref.2. Source: UniProtKB

synaptic transmission, cholinergic

Traceable author statement Ref.2. Source: UniProtKB

   Cellular_componentaxon

Inferred from electronic annotation. Source: Ensembl

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Traceable author statement Ref.2. Source: UniProtKB

perikaryon

Inferred from electronic annotation. Source: Ensembl

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionacetylcholine-activated cation-selective channel activity

Inferred from electronic annotation. Source: Ensembl

calcium channel activity

Inferred from electronic annotation. Source: UniProtKB-KW

receptor binding

Traceable author statement Ref.2. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 450426Neuronal acetylcholine receptor subunit alpha-10
PRO_0000000376

Regions

Topological domain25 – 237213Extracellular Potential
Transmembrane238 – 25821Helical; Potential
Transmembrane268 – 28821Helical; Potential
Transmembrane302 – 32221Helical; Potential
Topological domain323 – 428106Cytoplasmic Potential
Transmembrane429 – 44921Helical; Potential

Amino acid modifications

Glycosylation401N-linked (GlcNAc...) Potential
Glycosylation561N-linked (GlcNAc...) Potential
Disulfide bond154 ↔ 168 By similarity
Disulfide bond218 ↔ 219Associated with receptor activation By similarity

Natural variations

Natural variant3551E → A.
Corresponds to variant rs2231547 [ dbSNP | Ensembl ].
VAR_048172

Sequences

Sequence LengthMass (Da)Tools
Q9GZZ6 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 0A1DAD32D9069870

FASTA45049,705
        10         20         30         40         50         60 
MGLRSHHLSL GLLLLFLLPA ECLGAEGRLA LKLFRDLFAN YTSALRPVAD TDQTLNVTLE 

        70         80         90        100        110        120 
VTLSQIIDMD ERNQVLTLYL WIRQEWTDAY LRWDPNAYGG LDAIRIPSSL VWRPDIVLYN 

       130        140        150        160        170        180 
KADAQPPGSA STNVVLRHDG AVRWDAPAIT RSSCRVDVAA FPFDAQHCGL TFGSWTHGGH 

       190        200        210        220        230        240 
QLDVRPRGAA ASLADFVENV EWRVLGMPAR RRVLTYGCCS EPYPDVTFTL LLRRRAAAYV 

       250        260        270        280        290        300 
CNLLLPCVLI SLLAPLAFHL PADSGEKVSL GVTVLLALTV FQLLLAESMP PAESVPLIGK 

       310        320        330        340        350        360 
YYMATMTMVT FSTALTILIM NLHYCGPSVR PVPAWARALL LGHLARGLCV RERGEPCGQS 

       370        380        390        400        410        420 
RPPELSPSPQ SPEGGAGPPA GPCHEPRCLC RQEALLHHVA TIANTFRSHR AAQRCHEDWK 

       430        440        450 
RLARVMDRFF LAIFFSMALV MSLLVLVQAL 

« Hide

References

[1]"A novel human nicotinic receptor subunit, alpha10, that confers functionality to the alpha9-subunit."
Sgard F., Charpantier E., Bertrand S., Walker N., Caput D., Graham D., Bertrand D., Besnard F.
Mol. Pharmacol. 61:150-159(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CHRNA9, TISSUE SPECIFICITY.
Tissue: Skeletal muscle.
[2]"Molecular cloning and mapping of the human nicotinic acetylcholine receptor alpha10 (CHRNA10)."
Lustig L.R., Peng H., Hiel H., Yamamoto T., Fuchs P.A.
Genomics 73:272-283(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Inner ear and Tonsil.
[3]"Identification and functional characterisation of a novel human neuronal nicotinic acetylcholine receptor subunit alpha 10."
Vandenberk I., van der Helm L., Nieuwstraten D.M., Verfaille C., Kremer A., van der Spek P., Masure S., Hoefnagel E., Yon J., Groot Kormelink P.J., Luyten W.H., Grantham C.J.
Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Characterization of the human nicotinic acetylcholine receptor subunit alpha (alpha) 9 (CHRNA9) and alpha (alpha) 10 (CHRNA10) in lymphocytes."
Peng H., Ferris R.L., Matthews T., Hiel H., Lopez-Albaitero A., Lustig L.R.
Life Sci. 76:263-280(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ278118 mRNA. Translation: CAC20435.1.
AF199235 mRNA. Translation: AAG00795.2.
AF327367 Genomic DNA. Translation: AAK14333.1.
AJ295237 mRNA. Translation: CAC16144.1.
RefSeqNP_065135.2. NM_020402.2.
UniGeneHs.732806.

3D structure databases

ProteinModelPortalQ9GZZ6.
SMRQ9GZZ6. Positions 28-449.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000250699.

Chemistry

BindingDBQ9GZZ6.
ChEMBLCHEMBL2109238.
DrugBankDB01161. Chloroprocaine.
DB00333. Methadone.
DB00184. Nicotine.
DB01090. Pentolinium.
DB00721. Procaine.
DB01116. Trimethaphan.
GuidetoPHARMACOLOGY470.

Polymorphism databases

DMDM14916526.

Proteomic databases

PaxDbQ9GZZ6.
PRIDEQ9GZZ6.

Protocols and materials databases

DNASU57053.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000250699; ENSP00000250699; ENSG00000129749.
GeneID57053.
KEGGhsa:57053.
UCSCuc001lyf.3. human.

Organism-specific databases

CTD57053.
GeneCardsGC11M003691.
HGNCHGNC:13800. CHRNA10.
HPAHPA060919.
MIM606372. gene.
neXtProtNX_Q9GZZ6.
PharmGKBPA26488.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG306809.
HOGENOMHOG000006756.
HOVERGENHBG003756.
InParanoidQ9GZZ6.
KOK04811.
OMADGAVRWD.
OrthoDBEOG72JWGV.
PhylomeDBQ9GZZ6.
TreeFamTF315605.

Gene expression databases

ArrayExpressQ9GZZ6.
BgeeQ9GZZ6.
CleanExHS_CHRNA10.
GenevestigatorQ9GZZ6.

Family and domain databases

Gene3D1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCHRNA10.
GenomeRNAi57053.
NextBio62824.
PROQ9GZZ6.
SOURCESearch...

Entry information

Entry nameACH10_HUMAN
AccessionPrimary (citable) accession number: Q9GZZ6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: March 1, 2001
Last modified: March 19, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM