Q9GZY0 (NXF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear RNA export factor 2

Alternative name(s):
Cancer/testis antigen 39
Short name=CT39
TAP-like protein 2
Short name=TAPL-2

Gene names

Name:	NXF2
Synonyms:	TAPL2
AND
Name:	NXF2B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	626 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the export of mRNA from the nucleus to the cytoplasm.
Subunit structure	Interacts with NXT1, NXT2, E1B-AP5, the REF proteins and with nucleoporins, Nup62, Nup153 and Nup214. Ref.8
Subcellular location	Nucleus › nucleoplasm. Cytoplasm. Note: Localized in the nucleoplasm and at the nuclear envelope. Shuttles between the nucleus and the cytoplasm.
Tissue specificity	Expressed almost exclusively in testis. Also expressed in several cancers. Ref.9
Domain	The NTF2 domain heterodimerizes with NXT1 and NXT2. The formation of NXF1/NXT1 heterodimers is required for NXF2-mediated nuclear mRNA export. The leucine-rich repeats and the NTF2-domain are essential for the export of mRNA from the nucleus. The C-terminal fragment, containing the TAP domain (also called UBA-like domain) and part of the NTF2-like domain, named the NPC-binding domain, mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF2 to the nuclear rim. The RNA-binding domain is a non-canonical RNP-type domain.
Sequence similarities	Belongs to the NXF family. Contains 4 LRR (leucine-rich) repeats. Contains 1 NTF2 domain. Contains 1 RRM (RNA recognition motif) domain. Contains 1 TAP-C domain.
Sequence caution	The sequence AAK31975.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA91154.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB75659.1 differs from that shown. Reason: The sequence differs from that shown due to either intron retention or a splicing event.

Ontologies

Keywords
Biological process	Transport mRNA transport
Cellular component	Cytoplasm Nucleus
Domain	Leucine-rich repeat Repeat
Ligand	RNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA export from nucleus Non-traceable author statement Ref.1. Source: UniProtKB multicellular organismal development Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	actin cytoskeleton Inferred from direct assay. Source: HPA cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nuclear RNA export factor complex Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	RNA binding Non-traceable author statement Ref.1. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 626	626	Nuclear RNA export factor 2		PRO_0000220533
Regions
	Domain	124 – 203	80	RRM
	Repeat	271 – 296	26	LRR 1
	Repeat	297 – 320	24	LRR 2
	Repeat	321 – 348	28	LRR 3
	Repeat	349 – 376	28	LRR 4
	Domain	391 – 541	151	NTF2
	Domain	570 – 625	56	TAP-C
	Compositional bias	528 – 531	4	Poly-Ser
	Compositional bias	561 – 564	4	Poly-Ser
Experimental info
	Mutagenesis	598	1	E → A: Has no effect on FG-nucleoporin binding.
	Mutagenesis	599	1	W → A: Suppresses FG-nucleoporin binding.
	Mutagenesis	600	1	N → A: Has no effect on FG-nucleoporin binding.

Sequences

	Sequence		Length	Mass (Da)
	Q9GZY0 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 319CFEDF3E67A163	FASTA	626	71,627
	10 20 30 40 50 60 MCSTLKKCGT YRTEVAECHD HGSTFQGRKK GGSSFRDNFD KRSCHYEHGG YERPPSHCQE 70 80 90 100 110 120 NDGSVEMRDV HKDQQLRHTP YSIRCERRMK WHSEDEIRIT TWRNRKPPER KMSQNTQDGY 130 140 150 160 170 180 TRNWFKVTIP YGIKYDKAWL MNSIQSHCSD RFTPVDFHYV RNRACFFVQD ASAASALKDV 190 200 210 220 230 240 SYKIYDDENQ KICIFVNHST APYSVKNKLK PGQMEMLKLT MNKRYNVSQQ ALDLQNLRFD 250 260 270 280 290 300 PDLMGRDIDI ILNRRNCMAA TLKIIERNFP ELLSLNLCNN KLYQLDGLSD ITEKAPKVKT 310 320 330 340 350 360 LNLSKNKLES AWELGKVKGL KLEELWLEGN PLCSTFSDQS AYVSAIRDCF PKLLRLDGRE 370 380 390 400 410 420 LSAPVIVDID SSETMKPCKE NFTGSETLKH LVLQFLQQYY SIYDSGDRQG LLGAYHDEAC 430 440 450 460 470 480 FSLAIPFDPK DSAPSSLCKY FEDSRNMKTL KDPYLKGELL RRTKRDIVDS LSALPKTQHD 490 500 510 520 530 540 LSSILVDVWC QTERMLCFSV NGVFKEVEGQ SQGSVLAFTR TFIATPGSSS SLCIVNDELF 550 560 570 580 590 600 VRDASPQETQ SAFSIPVSTL SSSSEPSLSQ EQQEMVQAFS AQSGMKLEWS QKCLQDNEWN 610 620 YTRAGQAFTM LQTEGKIPAE AFKQIS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture." Herold A., Suyama M., Rodrigues J.P., Braun I.C., Kutay U., Carmo-Fonseca M., Bork P., Izaurralde E. Mol. Cell. Biol. 20:8996-9008(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS. Tissue: Testis.
[2]	"NXF5, a novel member of the nuclear RNA export factor family, is lost in a male patient with a syndromic form of mental retardation." Jun L., Frints S., Duhamel H., Herold A., Abad-Rodrigues J., Dotti C., Izaurralde E., Marynen P., Froyen G. Curr. Biol. 11:1381-1391(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal brain.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[6]	"An abundance of X-linked genes expressed in spermatogonia." Wang P.J., McCarrey J.R., Yang F., Page D.C. Nat. Genet. 27:422-426(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-626. Tissue: Testis.
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 98-626.
[8]	"Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export." Black B.E., Levesque L., Holaska J.M., Wood T.C., Paschal B.M. Mol. Cell. Biol. 19:8616-8624(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NXT1.
[9]	"Five new human cancer-germline genes identified among 12 genes expressed in spermatogonia." Loriot A., Boon T., De Smet C. Int. J. Cancer 105:371-376(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, IDENTIFICATION AS A CANCER/TESTIS ANTIGEN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ277526 mRNA. Translation: CAC16588.1. AJ277659 mRNA. Translation: CAC20433.1. AL157436 mRNA. Translation: CAB75659.1. Sequence problems. Z81367 Genomic DNA. Translation: CAI42026.1. AL590069 Genomic DNA. Translation: CAD13494.1. BC015020 mRNA. Translation: AAH15020.1. AF285596 mRNA. Translation: AAK31975.1. Different initiation. AK000423 mRNA. Translation: BAA91154.1. Different initiation.
IPI	IPI00004931.
RefSeq	NP_001093156.1. NM_001099686.2. NP_071336.1. NM_022053.2.
UniGene	Hs.530358. Hs.648337. Hs.737538.
3D structure databases
ProteinModelPortal	Q9GZY0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9GZY0. 1 interaction.
STRING	9606.ENSP00000361835.
PTM databases
PhosphoSite	Q9GZY0.
Proteomic databases
PaxDb	Q9GZY0.
PRIDE	Q9GZY0.
Protocols and materials databases
DNASU	56001.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000330252; ENSP00000331471; ENSG00000185554. ENST00000372749; ENSP00000361835; ENSG00000185945. ENST00000372750; ENSP00000361836; ENSG00000185945. ENST00000372757; ENSP00000361843; ENSG00000185554. ENST00000372758; ENSP00000361844; ENSG00000185554. ENST00000395088; ENSP00000378523; ENSG00000185554. ENST00000412230; ENSP00000413087; ENSG00000185945. ENST00000457521; ENSP00000396447; ENSG00000185945.
GeneID	56001. 728343.
KEGG	hsa:56001. hsa:728343.
UCSC	uc004eiv.4. human.
Organism-specific databases
CTD	56001. 728343.
GeneCards	GC0XM101615. GC0XP101471.
HGNC	HGNC:8072. NXF2. HGNC:23984. NXF2B.
MIM	300315. gene.
neXtProt	NX_Q9GZY0.
PharmGKB	PA162398310.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG324438.
HOGENOM	HOG000236269.
HOVERGEN	HBG013199.
InParanoid	Q9GZY0.
KO	K14284.
OMA	NTQDGYT.
OrthoDB	EOG4PK27D.
PhylomeDB	Q9GZY0.
Gene expression databases
ArrayExpress	Q9GZY0.
Bgee	Q9GZY0.
CleanEx	HS_NXF2. HS_NXF2B.
Genevestigator	Q9GZY0.
GermOnline	ENSG00000185554. Homo sapiens. ENSG00000185945. Homo sapiens.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR001611. Leu-rich_rpt. IPR002075. NTF2. IPR018222. Nuclear_transport_factor_2_euk. IPR012677. Nucleotide-bd_a/b_plait. IPR005637. TAP_C_dom. IPR015245. Tap_RNA-bd. IPR009060. UBA-like. [Graphical view]
Pfam	PF02136. NTF2. 1 hit. PF09162. Tap-RNA_bind. 1 hit. PF03943. TAP_C. 1 hit. [Graphical view]
ProDom	PD043466. Tap_RNA_bd. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00804. TAP_C. 1 hit. [Graphical view]
SUPFAM	SSF46934. UBA_like. 1 hit.
PROSITE	PS51450. LRR. 3 hits. PS50177. NTF2_DOMAIN. 1 hit. PS50102. RRM. False negative. PS51281. TAP_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	61450.
SOURCE	Search...

Entry information

Entry name

NXF2_HUMAN

Accession

Primary (citable) accession number: Q9GZY0
Secondary accession number(s): Q9BXU4, Q9NSS1, Q9NX66

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2002
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 131 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.