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Q9GZY0 (NXF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear RNA export factor 2
Alternative name(s):
Cancer/testis antigen 39
Short name=CT39
TAP-like protein 2
Short name=TAPL-2
Gene names
Name:NXF2
Synonyms:TAPL2
AND
Name:NXF2B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length626 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the export of mRNA from the nucleus to the cytoplasm.

Subunit structure

Interacts with NXT1, NXT2, E1B-AP5, the REF proteins and with nucleoporins, Nup62, Nup153 and Nup214. Ref.8

Subcellular location

Nucleusnucleoplasm. Cytoplasm. Note: Localized in the nucleoplasm and at the nuclear envelope. Shuttles between the nucleus and the cytoplasm.

Tissue specificity

Expressed almost exclusively in testis. Also expressed in several cancers. Ref.9

Domain

The NTF2 domain heterodimerizes with NXT1 and NXT2. The formation of NXF1/NXT1 heterodimers is required for NXF2-mediated nuclear mRNA export.

The leucine-rich repeats and the NTF2-domain are essential for the export of mRNA from the nucleus.

The C-terminal fragment, containing the TAP domain (also called UBA-like domain) and part of the NTF2-like domain, named the NPC-binding domain, mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF2 to the nuclear rim.

The RNA-binding domain is a non-canonical RNP-type domain.

Sequence similarities

Belongs to the NXF family.

Contains 4 LRR (leucine-rich) repeats.

Contains 1 NTF2 domain.

Contains 1 RRM (RNA recognition motif) domain.

Contains 1 TAP-C domain.

Sequence caution

The sequence AAK31975.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA91154.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAB75659.1 differs from that shown. Reason: The sequence differs from that shown due to either intron retention or a splicing event.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 626626Nuclear RNA export factor 2
PRO_0000220533

Regions

Domain124 – 20380RRM
Repeat271 – 29626LRR 1
Repeat297 – 32024LRR 2
Repeat321 – 34828LRR 3
Repeat349 – 37628LRR 4
Domain391 – 541151NTF2
Domain570 – 62556TAP-C
Compositional bias528 – 5314Poly-Ser
Compositional bias561 – 5644Poly-Ser

Experimental info

Mutagenesis5981E → A: Has no effect on FG-nucleoporin binding.
Mutagenesis5991W → A: Suppresses FG-nucleoporin binding.
Mutagenesis6001N → A: Has no effect on FG-nucleoporin binding.

Sequences

Sequence LengthMass (Da)Tools
Q9GZY0 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 319CFEDF3E67A163

FASTA62671,627
        10         20         30         40         50         60 
MCSTLKKCGT YRTEVAECHD HGSTFQGRKK GGSSFRDNFD KRSCHYEHGG YERPPSHCQE 

        70         80         90        100        110        120 
NDGSVEMRDV HKDQQLRHTP YSIRCERRMK WHSEDEIRIT TWRNRKPPER KMSQNTQDGY 

       130        140        150        160        170        180 
TRNWFKVTIP YGIKYDKAWL MNSIQSHCSD RFTPVDFHYV RNRACFFVQD ASAASALKDV 

       190        200        210        220        230        240 
SYKIYDDENQ KICIFVNHST APYSVKNKLK PGQMEMLKLT MNKRYNVSQQ ALDLQNLRFD 

       250        260        270        280        290        300 
PDLMGRDIDI ILNRRNCMAA TLKIIERNFP ELLSLNLCNN KLYQLDGLSD ITEKAPKVKT 

       310        320        330        340        350        360 
LNLSKNKLES AWELGKVKGL KLEELWLEGN PLCSTFSDQS AYVSAIRDCF PKLLRLDGRE 

       370        380        390        400        410        420 
LSAPVIVDID SSETMKPCKE NFTGSETLKH LVLQFLQQYY SIYDSGDRQG LLGAYHDEAC 

       430        440        450        460        470        480 
FSLAIPFDPK DSAPSSLCKY FEDSRNMKTL KDPYLKGELL RRTKRDIVDS LSALPKTQHD 

       490        500        510        520        530        540 
LSSILVDVWC QTERMLCFSV NGVFKEVEGQ SQGSVLAFTR TFIATPGSSS SLCIVNDELF 

       550        560        570        580        590        600 
VRDASPQETQ SAFSIPVSTL SSSSEPSLSQ EQQEMVQAFS AQSGMKLEWS QKCLQDNEWN 

       610        620 
YTRAGQAFTM LQTEGKIPAE AFKQIS 

« Hide

References

« Hide 'large scale' references
[1]"TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture."
Herold A., Suyama M., Rodrigues J.P., Braun I.C., Kutay U., Carmo-Fonseca M., Bork P., Izaurralde E.
Mol. Cell. Biol. 20:8996-9008(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS.
Tissue: Testis.
[2]"NXF5, a novel member of the nuclear RNA export factor family, is lost in a male patient with a syndromic form of mental retardation."
Jun L., Frints S., Duhamel H., Herold A., Abad-Rodrigues J., Dotti C., Izaurralde E., Marynen P., Froyen G.
Curr. Biol. 11:1381-1391(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Fetal brain.
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[4]"The DNA sequence of the human X chromosome."
Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. expand/collapse author list , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skin.
[6]"An abundance of X-linked genes expressed in spermatogonia."
Wang P.J., McCarrey J.R., Yang F., Page D.C.
Nat. Genet. 27:422-426(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-626.
Tissue: Testis.
[7]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 98-626.
[8]"Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export."
Black B.E., Levesque L., Holaska J.M., Wood T.C., Paschal B.M.
Mol. Cell. Biol. 19:8616-8624(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NXT1.
[9]"Five new human cancer-germline genes identified among 12 genes expressed in spermatogonia."
Loriot A., Boon T., De Smet C.
Int. J. Cancer 105:371-376(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, IDENTIFICATION AS A CANCER/TESTIS ANTIGEN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ277526 mRNA. Translation: CAC16588.1.
AJ277659 mRNA. Translation: CAC20433.1.
AL157436 mRNA. Translation: CAB75659.1. Sequence problems.
Z81367 Genomic DNA. Translation: CAI42026.1.
AL590069 Genomic DNA. Translation: CAD13494.1.
BC015020 mRNA. Translation: AAH15020.1.
AF285596 mRNA. Translation: AAK31975.1. Different initiation.
AK000423 mRNA. Translation: BAA91154.1. Different initiation.
CCDSCCDS14497.1.
CCDS43979.1.
RefSeqNP_001093156.1. NM_001099686.2.
NP_071336.1. NM_022053.3.
UniGeneHs.530358.
Hs.648337.
Hs.737538.

3D structure databases

ProteinModelPortalQ9GZY0.
SMRQ9GZY0. Positions 123-365, 377-559, 566-622.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121026. 5 interactions.
IntActQ9GZY0. 1 interaction.
STRING9606.ENSP00000361835.

PTM databases

PhosphoSiteQ9GZY0.

Polymorphism databases

DMDM20978536.

Proteomic databases

PaxDbQ9GZY0.
PRIDEQ9GZY0.

Protocols and materials databases

DNASU56001.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000330252; ENSP00000331471; ENSG00000185554.
ENST00000372749; ENSP00000361835; ENSG00000185945.
ENST00000372750; ENSP00000361836; ENSG00000185945.
ENST00000372757; ENSP00000361843; ENSG00000185554.
ENST00000372758; ENSP00000361844; ENSG00000185554.
ENST00000395088; ENSP00000378523; ENSG00000185554.
ENST00000412230; ENSP00000413087; ENSG00000185945.
ENST00000457521; ENSP00000396447; ENSG00000185945.
GeneID56001.
728343.
KEGGhsa:56001.
hsa:728343.
UCSCuc004eiv.4. human.

Organism-specific databases

CTD56001.
728343.
GeneCardsGC0XM101615.
GC0XP101471.
HGNCHGNC:8072. NXF2.
HGNC:23984. NXF2B.
MIM300315. gene.
neXtProtNX_Q9GZY0.
PharmGKBPA162398310.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG324438.
HOGENOMHOG000236269.
HOVERGENHBG013199.
InParanoidQ9GZY0.
KOK14284.
OMARNCHYEH.
OrthoDBEOG7QZGB9.
PhylomeDBQ9GZY0.
TreeFamTF314566.

Gene expression databases

BgeeQ9GZY0.
CleanExHS_NXF2.
HS_NXF2B.
GenevestigatorQ9GZY0.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR001611. Leu-rich_rpt.
IPR002075. NTF2.
IPR018222. Nuclear_transport_factor_2_euk.
IPR012677. Nucleotide-bd_a/b_plait.
IPR005637. TAP_C_dom.
IPR015245. Tap_RNA-bd.
IPR009060. UBA-like.
[Graphical view]
PfamPF02136. NTF2. 1 hit.
PF09162. Tap-RNA_bind. 1 hit.
PF03943. TAP_C. 1 hit.
[Graphical view]
ProDomPD043466. Tap_RNA_bd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00804. TAP_C. 1 hit.
[Graphical view]
SUPFAMSSF46934. SSF46934. 1 hit.
PROSITEPS51450. LRR. 3 hits.
PS50177. NTF2_DOMAIN. 1 hit.
PS51281. TAP_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiNXF2.
NextBio61450.
PROQ9GZY0.
SOURCESearch...

Entry information

Entry nameNXF2_HUMAN
AccessionPrimary (citable) accession number: Q9GZY0
Secondary accession number(s): Q9BXU4, Q9NSS1, Q9NX66
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM