ID   ZN350_HUMAN             Reviewed;         532 AA.
AC   Q9GZX5; Q96G73; Q9HAQ4;
DT   23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 3.
DT   27-MAR-2024, entry version 181.
DE   RecName: Full=Zinc finger protein 350;
DE   AltName: Full=KRAB zinc finger protein ZFQR;
DE   AltName: Full=Zinc finger and BRCA1-interacting protein with a KRAB domain 1;
DE   AltName: Full=Zinc finger protein ZBRK1;
GN   Name=ZNF350; Synonyms=ZBRK1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], VARIANTS PRO-66 AND SER-501, FUNCTION, AND
RP   INTERACTION WITH BRCA1.
RX   PubMed=11090615; DOI=10.1016/s1097-2765(00)00075-7;
RA   Zheng L., Pan H., Li S., Flesken-Nikitin A., Chen L.P., Boyer G.T.,
RA   Lee H.W.;
RT   "Sequence-specific transcriptional corepressor function for BRCA1 through a
RT   novel zinc finger protein, ZBRK1.";
RL   Mol. Cell 6:757-768(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], VARIANT ILE-524, TISSUE SPECIFICITY, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=11161714; DOI=10.1006/excr.2000.5068;
RA   Ran Q., Wadhwa R., Bischof O., Venable S., Smith J.R., Pereira-Smith O.M.;
RT   "Characterization of a novel zinc finger gene with increased expression in
RT   nondividing normal human cells.";
RL   Exp. Cell Res. 263:156-162(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS PRO-66 AND SER-501.
RC   TISSUE=Placenta;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15057824; DOI=10.1038/nature02399;
RA   Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA   Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA   Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA   Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA   Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA   Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA   Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA   Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA   Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA   McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA   Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA   Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA   She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA   Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA   Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA   Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA   Rubin E.M., Lucas S.M.;
RT   "The DNA sequence and biology of human chromosome 19.";
RL   Nature 428:529-535(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   SUBCELLULAR LOCATION.
RX   PubMed=23665872; DOI=10.1007/s00018-013-1359-4;
RA   Wang W., Cai J., Wu Y., Hu L., Chen Z., Hu J., Chen Z., Li W., Guo M.,
RA   Huang Z.;
RT   "Novel activity of KRAB domain that functions to reinforce nuclear
RT   localization of KRAB-containing zinc finger proteins by interacting with
RT   KAP1.";
RL   Cell. Mol. Life Sci. 70:3947-3958(2013).
RN   [8]
RP   VARIANTS PRO-66; PRO-472; SER-501 AND ILE-524.
RX   PubMed=12872252; DOI=10.1002/humu.10238;
RA   Rutter J.L., Smith A.M., Davila M.R., Sigurdson A.J., Giusti R.M.,
RA   Pineda M.A., Doody M.M., Tucker M.A., Greene M.H., Zhang J.,
RA   Struewing J.P.;
RT   "Mutational analysis of the BRCA1-interacting genes ZNF350/ZBRK1 and
RT   BRIP1/BACH1 among BRCA1 and BRCA2-negative probands from breast-ovarian
RT   cancer families and among early-onset breast cancer cases and reference
RT   individuals.";
RL   Hum. Mutat. 22:121-128(2003).
RN   [9]
RP   INTERACTION WITH RNF11.
RX   PubMed=14755250; DOI=10.1038/sj.onc.1207319;
RA   Li H., Seth A.K.;
RT   "An RNF11: Smurf2 complex mediates ubiquitination of the AMSH protein.";
RL   Oncogene 23:1801-1808(2004).
CC   -!- FUNCTION: Transcriptional repressor. Binds to a specific sequence, 5'-
CC       GGGxxxCAGxxxTTT-3', within GADD45 intron 3.
CC       {ECO:0000269|PubMed:11090615}.
CC   -!- SUBUNIT: Interacts with BRCA1. Interacts with RNF11.
CC       {ECO:0000269|PubMed:11090615, ECO:0000269|PubMed:14755250}.
CC   -!- INTERACTION:
CC       Q9GZX5; P38398: BRCA1; NbExp=3; IntAct=EBI-396421, EBI-349905;
CC   -!- SUBCELLULAR LOCATION: Nucleus. Nucleus matrix. Note=Associated with the
CC       nuclear matrix.
CC   -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:11161714}.
CC   -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC       family. {ECO:0000305}.
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DR   EMBL; AF295096; AAG17439.1; -; mRNA.
DR   EMBL; AF309561; AAG25714.1; -; mRNA.
DR   EMBL; AK023467; BAB14583.1; -; mRNA.
DR   EMBL; AC011460; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471135; EAW72056.1; -; Genomic_DNA.
DR   EMBL; BC009921; AAH09921.1; -; mRNA.
DR   CCDS; CCDS12845.1; -.
DR   RefSeq; NP_067645.3; NM_021632.3.
DR   RefSeq; XP_016882587.1; XM_017027098.1.
DR   RefSeq; XP_016882588.1; XM_017027099.1.
DR   RefSeq; XP_016882589.1; XM_017027100.1.
DR   AlphaFoldDB; Q9GZX5; -.
DR   SMR; Q9GZX5; -.
DR   BioGRID; 121889; 22.
DR   CORUM; Q9GZX5; -.
DR   IntAct; Q9GZX5; 10.
DR   MINT; Q9GZX5; -.
DR   STRING; 9606.ENSP00000243644; -.
DR   iPTMnet; Q9GZX5; -.
DR   PhosphoSitePlus; Q9GZX5; -.
DR   BioMuta; ZNF350; -.
DR   DMDM; 313104255; -.
DR   EPD; Q9GZX5; -.
DR   jPOST; Q9GZX5; -.
DR   MassIVE; Q9GZX5; -.
DR   MaxQB; Q9GZX5; -.
DR   PaxDb; 9606-ENSP00000243644; -.
DR   PeptideAtlas; Q9GZX5; -.
DR   ProteomicsDB; 80166; -.
DR   Antibodypedia; 49600; 227 antibodies from 25 providers.
DR   DNASU; 59348; -.
DR   Ensembl; ENST00000243644.9; ENSP00000243644.3; ENSG00000256683.7.
DR   GeneID; 59348; -.
DR   KEGG; hsa:59348; -.
DR   MANE-Select; ENST00000243644.9; ENSP00000243644.3; NM_021632.4; NP_067645.3.
DR   UCSC; uc002pyd.4; human.
DR   AGR; HGNC:16656; -.
DR   CTD; 59348; -.
DR   DisGeNET; 59348; -.
DR   GeneCards; ZNF350; -.
DR   HGNC; HGNC:16656; ZNF350.
DR   HPA; ENSG00000256683; Low tissue specificity.
DR   MIM; 605422; gene.
DR   neXtProt; NX_Q9GZX5; -.
DR   OpenTargets; ENSG00000256683; -.
DR   PharmGKB; PA134972827; -.
DR   VEuPathDB; HostDB:ENSG00000256683; -.
DR   eggNOG; KOG1721; Eukaryota.
DR   GeneTree; ENSGT00940000162449; -.
DR   HOGENOM; CLU_002678_0_2_1; -.
DR   InParanoid; Q9GZX5; -.
DR   OMA; CHENDAF; -.
DR   OrthoDB; 5260927at2759; -.
DR   PhylomeDB; Q9GZX5; -.
DR   TreeFam; TF340593; -.
DR   PathwayCommons; Q9GZX5; -.
DR   Reactome; R-HSA-212436; Generic Transcription Pathway.
DR   Reactome; R-HSA-3899300; SUMOylation of transcription cofactors.
DR   SignaLink; Q9GZX5; -.
DR   BioGRID-ORCS; 59348; 11 hits in 1154 CRISPR screens.
DR   ChiTaRS; ZNF350; human.
DR   GeneWiki; ZNF350; -.
DR   GenomeRNAi; 59348; -.
DR   Pharos; Q9GZX5; Tbio.
DR   PRO; PR:Q9GZX5; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; Q9GZX5; Protein.
DR   Bgee; ENSG00000256683; Expressed in calcaneal tendon and 150 other cell types or tissues.
DR   ExpressionAtlas; Q9GZX5; baseline and differential.
DR   GO; GO:0016604; C:nuclear body; IDA:HPA.
DR   GO; GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0017053; C:transcription repressor complex; IDA:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0001162; F:RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR   GO; GO:0045892; P:negative regulation of DNA-templated transcription; IDA:UniProtKB.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IDA:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd07765; KRAB_A-box; 1.
DR   Gene3D; 6.10.140.140; -; 1.
DR   Gene3D; 3.30.160.60; Classic Zinc Finger; 8.
DR   InterPro; IPR001909; KRAB.
DR   InterPro; IPR036051; KRAB_dom_sf.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   PANTHER; PTHR23232; KRAB DOMAIN C2H2 ZINC FINGER; 1.
DR   PANTHER; PTHR23232:SF137; ZINC FINGER PROTEIN 8; 1.
DR   Pfam; PF01352; KRAB; 1.
DR   Pfam; PF00096; zf-C2H2; 6.
DR   Pfam; PF13465; zf-H2C2_2; 1.
DR   SMART; SM00349; KRAB; 1.
DR   SMART; SM00355; ZnF_C2H2; 8.
DR   SUPFAM; SSF57667; beta-beta-alpha zinc fingers; 4.
DR   SUPFAM; SSF109640; KRAB domain (Kruppel-associated box); 1.
DR   PROSITE; PS50805; KRAB; 1.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 8.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 8.
DR   Genevisible; Q9GZX5; HS.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat; Repressor;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..532
FT                   /note="Zinc finger protein 350"
FT                   /id="PRO_0000047546"
FT   DOMAIN          8..79
FT                   /note="KRAB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00119"
FT   ZN_FING         206..228
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         234..256
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         262..284
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         290..312
FT                   /note="C2H2-type 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         318..340
FT                   /note="C2H2-type 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         346..368
FT                   /note="C2H2-type 6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         374..396
FT                   /note="C2H2-type 7"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         402..424
FT                   /note="C2H2-type 8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          427..465
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        427..448
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        449..465
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         37
FT                   /note="M -> I (in dbSNP:rs4987241)"
FT                   /id="VAR_046718"
FT   VARIANT         66
FT                   /note="L -> P (in dbSNP:rs2278420)"
FT                   /evidence="ECO:0000269|PubMed:11090615,
FT                   ECO:0000269|PubMed:12872252, ECO:0000269|PubMed:14702039"
FT                   /id="VAR_019902"
FT   VARIANT         69
FT                   /note="I -> T (in dbSNP:rs4987042)"
FT                   /id="VAR_046719"
FT   VARIANT         132
FT                   /note="R -> C (in dbSNP:rs28997584)"
FT                   /id="VAR_046720"
FT   VARIANT         406
FT                   /note="E -> K (in dbSNP:rs3764539)"
FT                   /id="VAR_046721"
FT   VARIANT         472
FT                   /note="S -> P (in dbSNP:rs4986771)"
FT                   /evidence="ECO:0000269|PubMed:12872252"
FT                   /id="VAR_019903"
FT   VARIANT         501
FT                   /note="R -> S (in dbSNP:rs2278415)"
FT                   /evidence="ECO:0000269|PubMed:11090615,
FT                   ECO:0000269|PubMed:12872252, ECO:0000269|PubMed:14702039"
FT                   /id="VAR_019904"
FT   VARIANT         524
FT                   /note="V -> I (in dbSNP:rs4988337)"
FT                   /evidence="ECO:0000269|PubMed:11161714,
FT                   ECO:0000269|PubMed:12872252"
FT                   /id="VAR_019905"
SQ   SEQUENCE   532 AA;  60011 MW;  FEF12A92F95BAC8A CRC64;
     MIQAQESITL EDVAVDFTWE EWQLLGAAQK DLYRDVMLEN YSNLVAVGYQ ASKPDALFKL
     EQGEQLWTIE DGIHSGACSD IWKVDHVLER LQSESLVNRR KPCHEHDAFE NIVHCSKSQF
     LLGQNHDIFD LRGKSLKSNL TLVNQSKGYE IKNSVEFTGN GDSFLHANHE RLHTAIKFPA
     SQKLISTKSQ FISPKHQKTR KLEKHHVCSE CGKAFIKKSW LTDHQVMHTG EKPHRCSLCE
     KAFSRKFMLT EHQRTHTGEK PYECPECGKA FLKKSRLNIH QKTHTGEKPY ICSECGKGFI
     QKGNLIVHQR IHTGEKPYIC NECGKGFIQK TCLIAHQRFH TGKTPFVCSE CGKSCSQKSG
     LIKHQRIHTG EKPFECSECG KAFSTKQKLI VHQRTHTGER PYGCNECGKA FAYMSCLVKH
     KRIHTREKQE AAKVENPPAE RHSSLHTSDV MQEKNSANGA TTQVPSVAPQ TSLNISGLLA
     NRNVVLVGQP VVRCAASGDN RGFAQDRNLV NAVNVVVPSV INYVLFYVTE NP
//