Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Zinc finger protein 350

Gene

ZNF350

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Binds to a specific sequence, 5'-GGGxxxCAGxxxTTT-3', within GADD45 intron 3.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri206 – 22823C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri234 – 25623C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri262 – 28423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri290 – 31223C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri318 – 34023C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri346 – 36823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri374 – 39623C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri402 – 42423C2H2-type 8PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW
  3. RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
  4. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: UniProtKB
  2. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  3. regulation of transcription, DNA-templated Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_12627. Generic Transcription Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 350
Alternative name(s):
KRAB zinc finger protein ZFQR
Zinc finger and BRCA1-interacting protein with a KRAB domain 1
Zinc finger protein ZBRK1
Gene namesi
Name:ZNF350
Synonyms:ZBRK1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:16656. ZNF350.

Subcellular locationi

Nucleus. Nucleus matrix
Note: Associated with the nuclear matrix.

GO - Cellular componenti

  1. nuclear matrix Source: UniProtKB-SubCell
  2. nucleoplasm Source: HPA
  3. nucleus Source: UniProtKB
  4. transcriptional repressor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134972827.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 532532Zinc finger protein 350PRO_0000047546Add
BLAST

Proteomic databases

MaxQBiQ9GZX5.
PaxDbiQ9GZX5.
PRIDEiQ9GZX5.

PTM databases

PhosphoSiteiQ9GZX5.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiQ9GZX5.
CleanExiHS_ZNF350.
ExpressionAtlasiQ9GZX5. baseline and differential.
GenevestigatoriQ9GZX5.

Organism-specific databases

HPAiHPA001521.

Interactioni

Subunit structurei

Interacts with BRCA1. Interacts with RNF11.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BRCA1P383983EBI-396421,EBI-349905

Protein-protein interaction databases

BioGridi121889. 7 interactions.
IntActiQ9GZX5. 5 interactions.
MINTiMINT-1198135.
STRINGi9606.ENSP00000365906.

Structurei

3D structure databases

ProteinModelPortaliQ9GZX5.
SMRiQ9GZX5. Positions 8-49, 154-457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 7972KRABPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 8 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 KRAB domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri206 – 22823C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri234 – 25623C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri262 – 28423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri290 – 31223C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri318 – 34023C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri346 – 36823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri374 – 39623C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri402 – 42423C2H2-type 8PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00780000121952.
HOGENOMiHOG000234617.
HOVERGENiHBG018163.
InParanoidiQ9GZX5.
KOiK09228.
OMAiHDAFENI.
OrthoDBiEOG7KSX7Q.
PhylomeDBiQ9GZX5.
TreeFamiTF340593.

Family and domain databases

Gene3Di3.30.160.60. 8 hits.
InterProiIPR001909. Krueppel-associated_box.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 8 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 8 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9GZX5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQAQESITL EDVAVDFTWE EWQLLGAAQK DLYRDVMLEN YSNLVAVGYQ
60 70 80 90 100
ASKPDALFKL EQGEQLWTIE DGIHSGACSD IWKVDHVLER LQSESLVNRR
110 120 130 140 150
KPCHEHDAFE NIVHCSKSQF LLGQNHDIFD LRGKSLKSNL TLVNQSKGYE
160 170 180 190 200
IKNSVEFTGN GDSFLHANHE RLHTAIKFPA SQKLISTKSQ FISPKHQKTR
210 220 230 240 250
KLEKHHVCSE CGKAFIKKSW LTDHQVMHTG EKPHRCSLCE KAFSRKFMLT
260 270 280 290 300
EHQRTHTGEK PYECPECGKA FLKKSRLNIH QKTHTGEKPY ICSECGKGFI
310 320 330 340 350
QKGNLIVHQR IHTGEKPYIC NECGKGFIQK TCLIAHQRFH TGKTPFVCSE
360 370 380 390 400
CGKSCSQKSG LIKHQRIHTG EKPFECSECG KAFSTKQKLI VHQRTHTGER
410 420 430 440 450
PYGCNECGKA FAYMSCLVKH KRIHTREKQE AAKVENPPAE RHSSLHTSDV
460 470 480 490 500
MQEKNSANGA TTQVPSVAPQ TSLNISGLLA NRNVVLVGQP VVRCAASGDN
510 520 530
RGFAQDRNLV NAVNVVVPSV INYVLFYVTE NP
Length:532
Mass (Da):60,011
Last modified:November 30, 2010 - v3
Checksum:iFEF12A92F95BAC8A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti37 – 371M → I.
Corresponds to variant rs4987241 [ dbSNP | Ensembl ].
VAR_046718
Natural varianti66 – 661L → P.3 Publications
Corresponds to variant rs2278420 [ dbSNP | Ensembl ].
VAR_019902
Natural varianti69 – 691I → T.
Corresponds to variant rs4987042 [ dbSNP | Ensembl ].
VAR_046719
Natural varianti132 – 1321R → C.
Corresponds to variant rs28997584 [ dbSNP | Ensembl ].
VAR_046720
Natural varianti406 – 4061E → K.
Corresponds to variant rs3764539 [ dbSNP | Ensembl ].
VAR_046721
Natural varianti472 – 4721S → P.1 Publication
Corresponds to variant rs4986771 [ dbSNP | Ensembl ].
VAR_019903
Natural varianti501 – 5011R → S.3 Publications
Corresponds to variant rs2278415 [ dbSNP | Ensembl ].
VAR_019904
Natural varianti524 – 5241V → I.2 Publications
Corresponds to variant rs4988337 [ dbSNP | Ensembl ].
VAR_019905

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF295096 mRNA. Translation: AAG17439.1.
AF309561 mRNA. Translation: AAG25714.1.
AK023467 mRNA. Translation: BAB14583.1.
AC011460 Genomic DNA. No translation available.
CH471135 Genomic DNA. Translation: EAW72056.1.
BC009921 mRNA. Translation: AAH09921.1.
CCDSiCCDS12845.1.
RefSeqiNP_067645.3. NM_021632.3.
UniGeneiHs.407694.

Genome annotation databases

EnsembliENST00000243644; ENSP00000243644; ENSG00000256683.
GeneIDi59348.
KEGGihsa:59348.
UCSCiuc002pyd.3. human.

Polymorphism databases

DMDMi313104255.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF295096 mRNA. Translation: AAG17439.1.
AF309561 mRNA. Translation: AAG25714.1.
AK023467 mRNA. Translation: BAB14583.1.
AC011460 Genomic DNA. No translation available.
CH471135 Genomic DNA. Translation: EAW72056.1.
BC009921 mRNA. Translation: AAH09921.1.
CCDSiCCDS12845.1.
RefSeqiNP_067645.3. NM_021632.3.
UniGeneiHs.407694.

3D structure databases

ProteinModelPortaliQ9GZX5.
SMRiQ9GZX5. Positions 8-49, 154-457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121889. 7 interactions.
IntActiQ9GZX5. 5 interactions.
MINTiMINT-1198135.
STRINGi9606.ENSP00000365906.

PTM databases

PhosphoSiteiQ9GZX5.

Polymorphism databases

DMDMi313104255.

Proteomic databases

MaxQBiQ9GZX5.
PaxDbiQ9GZX5.
PRIDEiQ9GZX5.

Protocols and materials databases

DNASUi59348.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000243644; ENSP00000243644; ENSG00000256683.
GeneIDi59348.
KEGGihsa:59348.
UCSCiuc002pyd.3. human.

Organism-specific databases

CTDi59348.
GeneCardsiGC19M052470.
HGNCiHGNC:16656. ZNF350.
HPAiHPA001521.
MIMi605422. gene.
neXtProtiNX_Q9GZX5.
PharmGKBiPA134972827.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00780000121952.
HOGENOMiHOG000234617.
HOVERGENiHBG018163.
InParanoidiQ9GZX5.
KOiK09228.
OMAiHDAFENI.
OrthoDBiEOG7KSX7Q.
PhylomeDBiQ9GZX5.
TreeFamiTF340593.

Enzyme and pathway databases

ReactomeiREACT_12627. Generic Transcription Pathway.

Miscellaneous databases

GeneWikiiZNF350.
GenomeRNAii59348.
NextBioi65253.
PROiQ9GZX5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9GZX5.
CleanExiHS_ZNF350.
ExpressionAtlasiQ9GZX5. baseline and differential.
GenevestigatoriQ9GZX5.

Family and domain databases

Gene3Di3.30.160.60. 8 hits.
InterProiIPR001909. Krueppel-associated_box.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 8 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 8 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence-specific transcriptional corepressor function for BRCA1 through a novel zinc finger protein, ZBRK1."
    Zheng L., Pan H., Li S., Flesken-Nikitin A., Chen L.P., Boyer G.T., Lee H.W.
    Mol. Cell 6:757-768(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS PRO-66 AND SER-501, FUNCTION, INTERACTION WITH BRCA1.
  2. "Characterization of a novel zinc finger gene with increased expression in nondividing normal human cells."
    Ran Q., Wadhwa R., Bischof O., Venable S., Smith J.R., Pereira-Smith O.M.
    Exp. Cell Res. 263:156-162(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ILE-524, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS PRO-66 AND SER-501.
    Tissue: Placenta.
  4. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  7. "Novel activity of KRAB domain that functions to reinforce nuclear localization of KRAB-containing zinc finger proteins by interacting with KAP1."
    Wang W., Cai J., Wu Y., Hu L., Chen Z., Hu J., Chen Z., Li W., Guo M., Huang Z.
    Cell. Mol. Life Sci. 70:3947-3958(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Mutational analysis of the BRCA1-interacting genes ZNF350/ZBRK1 and BRIP1/BACH1 among BRCA1 and BRCA2-negative probands from breast-ovarian cancer families and among early-onset breast cancer cases and reference individuals."
    Rutter J.L., Smith A.M., Davila M.R., Sigurdson A.J., Giusti R.M., Pineda M.A., Doody M.M., Tucker M.A., Greene M.H., Zhang J., Struewing J.P.
    Hum. Mutat. 22:121-128(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS PRO-66; PRO-472; SER-501 AND ILE-524.
  9. "An RNF11: Smurf2 complex mediates ubiquitination of the AMSH protein."
    Li H., Seth A.K.
    Oncogene 23:1801-1808(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RNF11.

Entry informationi

Entry nameiZN350_HUMAN
AccessioniPrimary (citable) accession number: Q9GZX5
Secondary accession number(s): Q96G73, Q9HAQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 30, 2010
Last modified: February 4, 2015
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.