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Q9GZV8 (PRD14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PR domain zinc finger protein 14

EC=2.1.1.-
Alternative name(s):
PR domain-containing protein 14
Gene names
Name:PRDM14
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length571 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression By similarity. Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'. Ref.4 Ref.5

Subcellular location

Nucleus Ref.4.

Tissue specificity

Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level). Ref.4 Ref.5

Domain

The first 5 zinc fingers, but not the last one, are required for DNA-binding and transcriptional activity.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily.

Contains 6 C2H2-type zinc fingers.

Contains 1 SET domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Hoxa1P090223EBI-3957793,EBI-3957603From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 571571PR domain zinc finger protein 14
PRO_0000047771

Regions

Domain251 – 367117SET
Zinc finger400 – 42425C2H2-type 1; atypical
Zinc finger432 – 45524C2H2-type 2
Zinc finger461 – 48323C2H2-type 3
Zinc finger489 – 51123C2H2-type 4
Zinc finger517 – 54024C2H2-type 5
Zinc finger546 – 56823C2H2-type 6

Amino acid modifications

Modified residue791Phosphoserine Ref.6

Natural variations

Natural variant2441K → E. Ref.3
Corresponds to variant rs3750228 [ dbSNP | Ensembl ].
VAR_021895

Sequences

Sequence LengthMass (Da)Tools
Q9GZV8 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 60D5ACC96DADE500

FASTA57164,062
        10         20         30         40         50         60 
MALPRPSEAV PQDKVCYPPE SSPQNLAAYY TPFPSYGHYR NSLATVEEDF QPFRQLEAAA 

        70         80         90        100        110        120 
SAAPAMPPFP FRMAPPLLSP GLGLQREPLY DLPWYSKLPP WYPIPHVPRE VPPFLSSSHE 

       130        140        150        160        170        180 
YAGASSEDLG HQIIGGDNES GPCCGPDTLI PPPPADASLL PEGLRTSQLL PCSPSKQSED 

       190        200        210        220        230        240 
GPKPSNQEGK SPARFQFTEE DLHFVLYGVT PSLEHPASLH HAISGLLVPP DSSGSDSLPQ 

       250        260        270        280        290        300 
TLDKDSLQLP EGLCLMQTVF GEVPHFGVFC SSFIAKGVRF GPFQGKVVNA SEVKTYGDNS 

       310        320        330        340        350        360 
VMWEIFEDGH LSHFIDGKGG TGNWMSYVNC ARFPKEQNLV AVQCQGHIFY ESCKEIHQNQ 

       370        380        390        400        410        420 
ELLVWYGDCY EKFLDIPVSL QVTEPGKQPS GPSEESAEGY RCERCGKVFT YKYYRDKHLK 

       430        440        450        460        470        480 
YTPCVDKGDR KFPCSLCKRS FEKRDRLRIH ILHVHEKHRP HKCSTCGKCF SQSSSLNKHM 

       490        500        510        520        530        540 
RVHSGDRPYQ CVYCTKRFTA SSILRTHIRQ HSGEKPFKCK YCGKSFASHA AHDSHVRRSH 

       550        560        570 
KEDDGCSCSI CGKIFSDQET FYSHMKFHED Y 

« Hide

References

« Hide 'large scale' references
[1]"A family of novel PR-domain (PRDM) genes as candidate tumor suppressors."
Yang X.-H., Huang S.
Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-244.
Tissue: Ovary.
[4]"Gene amplification and overexpression of PRDM14 in breast cancers."
Nishikawa N., Toyota M., Suzuki H., Honma T., Fujikane T., Ohmura T., Nishidate T., Ohe-Toyota M., Maruyama R., Sonoda T., Sasaki Y., Urano T., Imai K., Hirata K., Tokino T.
Cancer Res. 67:9649-9657(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]"A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity."
Chia N.Y., Chan Y.S., Feng B., Lu X., Orlov Y.L., Moreau D., Kumar P., Yang L., Jiang J., Lau M.S., Huss M., Soh B.S., Kraus P., Li P., Lufkin T., Lim B., Clarke N.D., Bard F., Ng H.H.
Nature 468:316-320(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF319458 mRNA. Translation: AAG39635.1.
AK022595 mRNA. Translation: BAB14120.1.
BC052311 mRNA. Translation: AAH52311.1.
CCDSCCDS6206.1.
RefSeqNP_078780.1. NM_024504.3.
UniGeneHs.736037.

3D structure databases

ProteinModelPortalQ9GZV8.
SMRQ9GZV8. Positions 200-568.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122025. 4 interactions.
IntActQ9GZV8. 4 interactions.
MINTMINT-7971034.
STRING9606.ENSP00000276594.

PTM databases

PhosphoSiteQ9GZV8.

Polymorphism databases

DMDM17368925.

Proteomic databases

PaxDbQ9GZV8.
PRIDEQ9GZV8.

Protocols and materials databases

DNASU63978.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000276594; ENSP00000276594; ENSG00000147596.
GeneID63978.
KEGGhsa:63978.
UCSCuc003xym.3. human.

Organism-specific databases

CTD63978.
GeneCardsGC08M070963.
HGNCHGNC:14001. PRDM14.
HPAHPA019094.
MIM611781. gene.
neXtProtNX_Q9GZV8.
PharmGKBPA33712.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG297028.
HOGENOMHOG000115684.
HOVERGENHBG053668.
InParanoidQ9GZV8.
OMAPREVPHF.
OrthoDBEOG7B5WW5.
PhylomeDBQ9GZV8.
TreeFamTF106412.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.
SignaLinkQ9GZV8.

Gene expression databases

ArrayExpressQ9GZV8.
BgeeQ9GZV8.
CleanExHS_PRDM14.
GenevestigatorQ9GZV8.

Family and domain databases

Gene3D3.30.160.60. 3 hits.
InterProIPR001214. SET_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00856. SET. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00317. SET. 1 hit.
SM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEPS50280. SET. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi63978.
NextBio65790.
PROQ9GZV8.
SOURCESearch...

Entry information

Entry namePRD14_HUMAN
AccessionPrimary (citable) accession number: Q9GZV8
Secondary accession number(s): Q86UX9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM