Q9GZV8 (PRD14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PR domain zinc finger protein 14

Alternative name(s):
PR domain-containing protein 14

Gene names

Name:

PRDM14

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression By similarity. Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'. Ref.4 Ref.5
Subcellular location	Nucleus Ref.4.
Tissue specificity	Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level). Ref.4 Ref.5
Domain	The first 5 zinc fingers, but not the last one, are required for DNA-binding and transcriptional activity.
Sequence similarities	Contains 6 C2H2-type zinc fingers. Contains 1 SET domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell fate specification Inferred from electronic annotation. Source: Compara cell morphogenesis Inferred from electronic annotation. Source: Compara fertilization Inferred from electronic annotation. Source: Compara germ cell development Inferred from electronic annotation. Source: Compara homeostasis of number of cells within a tissue Inferred from electronic annotation. Source: Compara regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Hoxa1	P09022	3	EBI-3957793,EBI-3957603	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	PR domain zinc finger protein 14		PRO_0000047771
Regions
	Domain	253 – 371	119	SET
	Zinc finger	400 – 424	25	C2H2-type 1; atypical
	Zinc finger	432 – 455	24	C2H2-type 2
	Zinc finger	461 – 483	23	C2H2-type 3
	Zinc finger	489 – 511	23	C2H2-type 4
	Zinc finger	517 – 540	24	C2H2-type 5
	Zinc finger	546 – 568	23	C2H2-type 6
Amino acid modifications
	Modified residue	79	1	Phosphoserine Ref.6
Natural variations
	Natural variant	244	1	K → E. Ref.3 Corresponds to variant rs3750228 [ dbSNP | Ensembl ].		VAR_021895

Sequences

	Sequence		Length	Mass (Da)
	Q9GZV8 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 60D5ACC96DADE500	FASTA	571	64,062
	10 20 30 40 50 60 MALPRPSEAV PQDKVCYPPE SSPQNLAAYY TPFPSYGHYR NSLATVEEDF QPFRQLEAAA 70 80 90 100 110 120 SAAPAMPPFP FRMAPPLLSP GLGLQREPLY DLPWYSKLPP WYPIPHVPRE VPPFLSSSHE 130 140 150 160 170 180 YAGASSEDLG HQIIGGDNES GPCCGPDTLI PPPPADASLL PEGLRTSQLL PCSPSKQSED 190 200 210 220 230 240 GPKPSNQEGK SPARFQFTEE DLHFVLYGVT PSLEHPASLH HAISGLLVPP DSSGSDSLPQ 250 260 270 280 290 300 TLDKDSLQLP EGLCLMQTVF GEVPHFGVFC SSFIAKGVRF GPFQGKVVNA SEVKTYGDNS 310 320 330 340 350 360 VMWEIFEDGH LSHFIDGKGG TGNWMSYVNC ARFPKEQNLV AVQCQGHIFY ESCKEIHQNQ 370 380 390 400 410 420 ELLVWYGDCY EKFLDIPVSL QVTEPGKQPS GPSEESAEGY RCERCGKVFT YKYYRDKHLK 430 440 450 460 470 480 YTPCVDKGDR KFPCSLCKRS FEKRDRLRIH ILHVHEKHRP HKCSTCGKCF SQSSSLNKHM 490 500 510 520 530 540 RVHSGDRPYQ CVYCTKRFTA SSILRTHIRQ HSGEKPFKCK YCGKSFASHA AHDSHVRRSH 550 560 570 KEDDGCSCSI CGKIFSDQET FYSHMKFHED Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A family of novel PR-domain (PRDM) genes as candidate tumor suppressors." Yang X.-H., Huang S. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-244. Tissue: Ovary.
[4]	"Gene amplification and overexpression of PRDM14 in breast cancers." Nishikawa N., Toyota M., Suzuki H., Honma T., Fujikane T., Ohmura T., Nishidate T., Ohe-Toyota M., Maruyama R., Sonoda T., Sasaki Y., Urano T., Imai K., Hirata K., Tokino T. Cancer Res. 67:9649-9657(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	"A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity." Chia N.Y., Chan Y.S., Feng B., Lu X., Orlov Y.L., Moreau D., Kumar P., Yang L., Jiang J., Lau M.S., Huss M., Soh B.S., Kraus P., Li P., Lufkin T., Lim B., Clarke N.D., Bard F., Ng H.H. Nature 468:316-320(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF319458 mRNA. Translation: AAG39635.1. AK022595 mRNA. Translation: BAB14120.1. BC052311 mRNA. Translation: AAH52311.1.
IPI	IPI00001567.
RefSeq	NP_078780.1. NM_024504.3.
UniGene	Hs.736037.
3D structure databases
ProteinModelPortal	Q9GZV8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9GZV8. 1 interaction.
STRING	9606.ENSP00000276594.
PTM databases
PhosphoSite	Q9GZV8.
Polymorphism databases
DMDM	17368925.
Proteomic databases
PaxDb	Q9GZV8.
PRIDE	Q9GZV8.
Protocols and materials databases
DNASU	63978.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000276594; ENSP00000276594; ENSG00000147596.
GeneID	63978.
KEGG	hsa:63978.
UCSC	uc003xym.3. human.
Organism-specific databases
CTD	63978.
GeneCards	GC08M070963.
HGNC	HGNC:14001. PRDM14.
HPA	HPA019094.
MIM	611781. gene.
neXtProt	NX_Q9GZV8.
PharmGKB	PA33712.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG297028.
HOGENOM	HOG000115684.
HOVERGEN	HBG053668.
InParanoid	Q9GZV8.
OMA	PREVPHF.
OrthoDB	EOG434W65.
PhylomeDB	Q9GZV8.
Gene expression databases
ArrayExpress	Q9GZV8.
Bgee	Q9GZV8.
CleanEx	HS_PRDM14.
Genevestigator	Q9GZV8.
GermOnline	ENSG00000147596. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 3 hits.
InterPro	IPR001214. SET_dom. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF00856. SET. 1 hit. [Graphical view]
SMART	SM00317. SET. 1 hit. SM00355. ZnF_C2H2. 6 hits. [Graphical view]
PROSITE	PS50280. SET. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 5 hits. PS50157. ZINC_FINGER_C2H2_2. 6 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	63978.
NextBio	65790.
SOURCE	Search...

Entry information

Entry name

PRD14_HUMAN

Accession

Primary (citable) accession number: Q9GZV8
Secondary accession number(s): Q86UX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.