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Protein

PR domain zinc finger protein 14

Gene

PRDM14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression (By similarity). Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri400 – 42425C2H2-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri432 – 45524C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri461 – 48323C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri489 – 51123C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri517 – 54024C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri546 – 56823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. methyltransferase activity Source: UniProtKB-KW

GO - Biological processi

  1. cell fate specification Source: Ensembl
  2. cell morphogenesis Source: Ensembl
  3. fertilization Source: Ensembl
  4. germ cell development Source: Ensembl
  5. germ-line stem cell maintenance Source: Ensembl
  6. histone H3-R26 methylation Source: Ensembl
  7. homeostasis of number of cells within a tissue Source: Ensembl
  8. inner cell mass cell fate commitment Source: Ensembl
  9. negative regulation of fibroblast growth factor receptor signaling pathway Source: Ensembl
  10. regulation of DNA methylation Source: Ensembl
  11. regulation of gene expression, epigenetic Source: Ensembl
  12. regulation of transcription, DNA-templated Source: UniProtKB-KW
  13. somatic stem cell maintenance Source: Reactome
  14. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

ReactomeiREACT_263856. Transcriptional regulation of pluripotent stem cells.
SignaLinkiQ9GZV8.

Names & Taxonomyi

Protein namesi
Recommended name:
PR domain zinc finger protein 14 (EC:2.1.1.-)
Alternative name(s):
PR domain-containing protein 14
Gene namesi
Name:PRDM14
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:14001. PRDM14.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33712.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 571571PR domain zinc finger protein 14PRO_0000047771Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei79 – 791Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9GZV8.
PRIDEiQ9GZV8.

PTM databases

PhosphoSiteiQ9GZV8.

Expressioni

Tissue specificityi

Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level).2 Publications

Gene expression databases

BgeeiQ9GZV8.
CleanExiHS_PRDM14.
ExpressionAtlasiQ9GZV8. baseline and differential.
GenevestigatoriQ9GZV8.

Organism-specific databases

HPAiHPA019094.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Hoxa1P090223EBI-3957793,EBI-3957603From a different organism.

Protein-protein interaction databases

BioGridi122025. 20 interactions.
IntActiQ9GZV8. 4 interactions.
MINTiMINT-7971034.
STRINGi9606.ENSP00000276594.

Structurei

3D structure databases

ProteinModelPortaliQ9GZV8.
SMRiQ9GZV8. Positions 243-568.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini251 – 367117SETPROSITE-ProRule annotationAdd
BLAST

Domaini

The first 5 zinc fingers, but not the last one, are required for DNA-binding and transcriptional activity.

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily.PROSITE-ProRule annotation
Contains 6 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 SET domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri400 – 42425C2H2-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri432 – 45524C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri461 – 48323C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri489 – 51123C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri517 – 54024C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri546 – 56823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG297028.
GeneTreeiENSGT00770000120502.
HOGENOMiHOG000115684.
HOVERGENiHBG053668.
InParanoidiQ9GZV8.
OMAiPREVPHF.
OrthoDBiEOG7B5WW5.
PhylomeDBiQ9GZV8.
TreeFamiTF106412.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR001214. SET_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00856. SET. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00317. SET. 1 hit.
SM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS50280. SET. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9GZV8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPRPSEAV PQDKVCYPPE SSPQNLAAYY TPFPSYGHYR NSLATVEEDF
60 70 80 90 100
QPFRQLEAAA SAAPAMPPFP FRMAPPLLSP GLGLQREPLY DLPWYSKLPP
110 120 130 140 150
WYPIPHVPRE VPPFLSSSHE YAGASSEDLG HQIIGGDNES GPCCGPDTLI
160 170 180 190 200
PPPPADASLL PEGLRTSQLL PCSPSKQSED GPKPSNQEGK SPARFQFTEE
210 220 230 240 250
DLHFVLYGVT PSLEHPASLH HAISGLLVPP DSSGSDSLPQ TLDKDSLQLP
260 270 280 290 300
EGLCLMQTVF GEVPHFGVFC SSFIAKGVRF GPFQGKVVNA SEVKTYGDNS
310 320 330 340 350
VMWEIFEDGH LSHFIDGKGG TGNWMSYVNC ARFPKEQNLV AVQCQGHIFY
360 370 380 390 400
ESCKEIHQNQ ELLVWYGDCY EKFLDIPVSL QVTEPGKQPS GPSEESAEGY
410 420 430 440 450
RCERCGKVFT YKYYRDKHLK YTPCVDKGDR KFPCSLCKRS FEKRDRLRIH
460 470 480 490 500
ILHVHEKHRP HKCSTCGKCF SQSSSLNKHM RVHSGDRPYQ CVYCTKRFTA
510 520 530 540 550
SSILRTHIRQ HSGEKPFKCK YCGKSFASHA AHDSHVRRSH KEDDGCSCSI
560 570
CGKIFSDQET FYSHMKFHED Y
Length:571
Mass (Da):64,062
Last modified:March 1, 2001 - v1
Checksum:i60D5ACC96DADE500
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti244 – 2441K → E.1 Publication
Corresponds to variant rs3750228 [ dbSNP | Ensembl ].
VAR_021895

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF319458 mRNA. Translation: AAG39635.1.
AK022595 mRNA. Translation: BAB14120.1.
BC052311 mRNA. Translation: AAH52311.1.
CCDSiCCDS6206.1.
RefSeqiNP_078780.1. NM_024504.3.
UniGeneiHs.736037.

Genome annotation databases

EnsembliENST00000276594; ENSP00000276594; ENSG00000147596.
GeneIDi63978.
KEGGihsa:63978.
UCSCiuc003xym.3. human.

Polymorphism databases

DMDMi17368925.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF319458 mRNA. Translation: AAG39635.1.
AK022595 mRNA. Translation: BAB14120.1.
BC052311 mRNA. Translation: AAH52311.1.
CCDSiCCDS6206.1.
RefSeqiNP_078780.1. NM_024504.3.
UniGeneiHs.736037.

3D structure databases

ProteinModelPortaliQ9GZV8.
SMRiQ9GZV8. Positions 243-568.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122025. 20 interactions.
IntActiQ9GZV8. 4 interactions.
MINTiMINT-7971034.
STRINGi9606.ENSP00000276594.

PTM databases

PhosphoSiteiQ9GZV8.

Polymorphism databases

DMDMi17368925.

Proteomic databases

PaxDbiQ9GZV8.
PRIDEiQ9GZV8.

Protocols and materials databases

DNASUi63978.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000276594; ENSP00000276594; ENSG00000147596.
GeneIDi63978.
KEGGihsa:63978.
UCSCiuc003xym.3. human.

Organism-specific databases

CTDi63978.
GeneCardsiGC08M070963.
HGNCiHGNC:14001. PRDM14.
HPAiHPA019094.
MIMi611781. gene.
neXtProtiNX_Q9GZV8.
PharmGKBiPA33712.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG297028.
GeneTreeiENSGT00770000120502.
HOGENOMiHOG000115684.
HOVERGENiHBG053668.
InParanoidiQ9GZV8.
OMAiPREVPHF.
OrthoDBiEOG7B5WW5.
PhylomeDBiQ9GZV8.
TreeFamiTF106412.

Enzyme and pathway databases

ReactomeiREACT_263856. Transcriptional regulation of pluripotent stem cells.
SignaLinkiQ9GZV8.

Miscellaneous databases

GenomeRNAii63978.
NextBioi65790.
PROiQ9GZV8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9GZV8.
CleanExiHS_PRDM14.
ExpressionAtlasiQ9GZV8. baseline and differential.
GenevestigatoriQ9GZV8.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR001214. SET_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00856. SET. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00317. SET. 1 hit.
SM00355. ZnF_C2H2. 6 hits.
[Graphical view]
PROSITEiPS50280. SET. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A family of novel PR-domain (PRDM) genes as candidate tumor suppressors."
    Yang X.-H., Huang S.
    Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-244.
    Tissue: Ovary.
  4. Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity."
    Chia N.Y., Chan Y.S., Feng B., Lu X., Orlov Y.L., Moreau D., Kumar P., Yang L., Jiang J., Lau M.S., Huss M., Soh B.S., Kraus P., Li P., Lufkin T., Lim B., Clarke N.D., Bard F., Ng H.H.
    Nature 468:316-320(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRD14_HUMAN
AccessioniPrimary (citable) accession number: Q9GZV8
Secondary accession number(s): Q86UX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: March 1, 2001
Last modified: April 1, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.