Q9GZV4 (IF5A2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eukaryotic translation initiation factor 5A-2
Short name=eIF-5A-2
Short name=eIF-5A2

Alternative name(s):
Eukaryotic initiation factor 5A isoform 2

Gene names

Name:

EIF5A2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation By similarity. Ref.7
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Nucleus › nuclear pore complex By similarity. Note: Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions By similarity.
Tissue specificity	Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells. Ref.2 Ref.8
Post-translational modification	eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
Sequence similarities	Belongs to the eIF-5A family.

Ontologies

Keywords
Biological process	Protein biosynthesis Protein transport Translocation Transport mRNA transport
Cellular component	Cytoplasm Endoplasmic reticulum Membrane Nuclear pore complex Nucleus
Coding sequence diversity	Polymorphism
Ligand	RNA-binding
Molecular function	Elongation factor
PTM	Hypusine
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-lysine modification to hypusine Traceable author statement. Source: Reactome polyamine homeostasis Non-traceable author statement Ref.2. Source: UniProtKB positive regulation of cell proliferation Inferred from genetic interaction Ref.7. Source: UniProtKB positive regulation of translational elongation Inferred from electronic annotation. Source: InterPro positive regulation of translational termination Inferred from electronic annotation. Source: InterPro post-translational protein modification Traceable author statement. Source: Reactome protein transport Inferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Non-traceable author statement Ref.2. Source: UniProtKB translational frameshifting Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Traceable author statement. Source: Reactome endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear pore Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ribosome binding Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 153	153	Eukaryotic translation initiation factor 5A-2		PRO_0000229764
Amino acid modifications
	Modified residue	2	1	Blocked amino end (Ala) By similarity
	Modified residue	50	1	Hypusine
Natural variations
	Natural variant	42	1	E → D. Ref.9		VAR_027943

Sequences

	Sequence		Length	Mass (Da)
	Q9GZV4 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 632BFE7F6DB073BD	FASTA	153	16,793
	10 20 30 40 50 60 MADEIDFTTG DAGASSTYPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG 70 80 90 100 110 120 IDIFTGKKYE DICPSTHNMD VPNIKRNDYQ LICIQDGYLS LLTETGEVRE DLKLPEGELG 130 140 150 KEIEGKYNAG EDVQVSVMCA MSEEYAVAIK PCK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a novel candidate oncogene within a frequently amplified region at 3q26 in ovarian cancer." Guan X.-Y.Y., Sham J.S.T., Tang T.C.-M., Fang Y., Huo K.-K., Yang J.-M. Cancer Res. 61:3806-3809(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovarian carcinoma.
[2]	"Human eIF5A2 on chromosome 3q25-q27 is a phylogenetically conserved vertebrate variant of eukaryotic translation initiation factor 5A with tissue-specific expression." Jenkins Z.A., Haag P.G., Johansson H.E. Genomics 71:101-109(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
[3]	"Expression of eIF5A genes in human cancer cells." Clement P.M.J., Jenkins Z.A., Park M.H., Johansson H.E. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[7]	"Identification and characterization of eukaryotic initiation factor 5A-2." Clement P.M.J., Henderson C.A., Jenkins Z.A., Smit-McBride Z., Wolff E.C., Hershey J.W., Park M.H., Johansson H.E. Eur. J. Biochem. 270:4254-4263(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, HYPUSINE AT LYS-50.
[8]	"Differential expression of eIF5A-1 and eIF5A-2 in human cancer cells." Clement P.M.J., Johansson H.E., Wolff E.C., Park M.H. FEBS J. 273:1102-1114(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	"Screening the SPO11 and EIF5A2 genes in a population of infertile men." Christensen G.L., Ivanov I.P., Atkins J.F., Mielnik A., Schlegel P.N., Carrell D.T. Fertil. Steril. 84:758-760(2005) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT ASP-42.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF262027 mRNA. Translation: AAF98810.1. AF293387, AF293386 Genomic DNA. Translation: AAG23176.1. AY205258 mRNA. Translation: AAO18676.1. AY205259 mRNA. Translation: AAO18677.1. AY205260 mRNA. Translation: AAO18678.1. AY205261 mRNA. Translation: AAO18679.1. AK311962 mRNA. Translation: BAG34902.1. CH471052 Genomic DNA. Translation: EAW78501.1. BC036072 mRNA. Translation: AAH36072.1.
IPI	IPI00006935.
RefSeq	NP_065123.1. NM_020390.5.
UniGene	Hs.164144.
3D structure databases
ProteinModelPortal	Q9GZV4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9GZV4. 8 interactions.
MINT	MINT-1453868.
STRING	9606.ENSP00000295822.
PTM databases
PhosphoSite	Q9GZV4.
Polymorphism databases
DMDM	74762725.
Proteomic databases
PaxDb	Q9GZV4.
PRIDE	Q9GZV4.
Protocols and materials databases
DNASU	56648.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000295822; ENSP00000295822; ENSG00000163577.
GeneID	56648.
KEGG	hsa:56648.
UCSC	uc003fhd.3. human.
Organism-specific databases
CTD	56648.
GeneCards	GC03M170607.
HGNC	HGNC:3301. EIF5A2.
HPA	HPA029090.
MIM	605782. gene.
neXtProt	NX_Q9GZV4.
PharmGKB	PA27727.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0231.
HOGENOM	HOG000106270.
HOVERGEN	HBG001104.
InParanoid	Q9GZV4.
KO	K03263.
OMA	GHAKAHI.
OrthoDB	EOG41JZDF.
PhylomeDB	Q9GZV4.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins.
Gene expression databases
ArrayExpress	Q9GZV4.
Bgee	Q9GZV4.
CleanEx	HS_EIF5A2.
Genevestigator	Q9GZV4.
GermOnline	ENSG00000163577. Homo sapiens.
Family and domain databases
Gene3D	2.30.30.30. 1 hit. 2.40.50.140. 1 hit.
InterPro	IPR005824. KOW. IPR012340. NA-bd_OB-fold. IPR014722. Rib_L2_dom2. IPR019769. Trans_elong_IF5A_hypusine_site. IPR001884. Transl_elong_IF5A. IPR020189. Transl_elong_IF5A_C. IPR008991. Translation_prot_SH3-like. [Graphical view]
PANTHER	PTHR11673. PTHR11673. 1 hit.
Pfam	PF01287. eIF-5a. 1 hit. PF00467. KOW. 1 hit. [Graphical view]
PIRSF	PIRSF003025. eIF5A. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF50104. Transl_SH3_like. 1 hit.
TIGRFAMs	TIGR00037. eIF_5A. 1 hit.
PROSITE	PS00302. IF5A_HYPUSINE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	EIF5A2. human.
GenomeRNAi	56648.
NextBio	62085.
SOURCE	Search...

Entry information

Entry name

IF5A2_HUMAN

Accession

Primary (citable) accession number: Q9GZV4
Secondary accession number(s): B2R4V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 109 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.