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Q9GZS9 (CHST5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carbohydrate sulfotransferase 5

EC=2.8.2.-
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-alpha
Short name=GST4-alpha
Intestinal N-acetylglucosamine-6-O-sulfotransferase
Short name=I-GlcNAc6ST
Short name=Intestinal GlcNAc-6-sulfotransferase
Short name=hIGn6ST
N-acetylglucosamine 6-O-sulfotransferase 3
Short name=GlcNAc6ST-3
Short name=Gn6st-3
Gene names
Name:CHST5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length411 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location. Ref.1 Ref.5 Ref.7 Ref.8

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein Probable. Note: Golgi membrane, early secretory pathway. Ref.9

Tissue specificity

Predominantly expressed in small and large intestines and colon. Weakly expressed in lymphocytes. Not expressed in other tissues. Down-regulated in colonic adenocarcinomas. Ref.1 Ref.2 Ref.5 Ref.6 Ref.8

Sequence similarities

Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Sequence caution

The sequence AAD56000.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAD56001.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentGolgi apparatus
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   Molecular functionTransferase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processN-acetylglucosamine metabolic process

Inferred from direct assay Ref.1Ref.7. Source: UniProtKB

carbohydrate metabolic process

Traceable author statement. Source: Reactome

glycosaminoglycan metabolic process

Traceable author statement. Source: Reactome

keratan sulfate biosynthetic process

Traceable author statement. Source: Reactome

keratan sulfate metabolic process

Traceable author statement. Source: Reactome

protein sulfation

Traceable author statement Ref.1. Source: ProtInc

small molecule metabolic process

Traceable author statement. Source: Reactome

sulfur compound metabolic process

Inferred from direct assay Ref.1. Source: UniProtKB

   Cellular_componentGolgi apparatus

Inferred from direct assay Ref.9. Source: UniProtKB

Golgi membrane

Traceable author statement. Source: Reactome

integral component of membrane

Non-traceable author statement Ref.1. Source: UniProtKB

intrinsic component of Golgi membrane

Non-traceable author statement Ref.9. Source: UniProtKB

   Molecular_functionN-acetylglucosamine 6-O-sulfotransferase activity

Inferred from direct assay Ref.1. Source: UniProtKB

sulfotransferase activity

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9GZS9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9GZS9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: MGMRARVPKVAHSTRR → MSRHLPWICDQRCSSPSSPGRW

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 411411Carbohydrate sulfotransferase 5
PRO_0000085195

Regions

Topological domain1 – 3030Cytoplasmic Potential
Transmembrane31 – 4818Helical; Signal-anchor for type II membrane protein; Potential
Topological domain49 – 411363Lumenal Potential
Nucleotide binding71 – 777PAPS By similarity
Nucleotide binding224 – 2329PAPS By similarity

Amino acid modifications

Glycosylation1381N-linked (GlcNAc...) Potential
Glycosylation3271N-linked (GlcNAc...) Potential
Glycosylation3501N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 1616MGMRA…HSTRR → MSRHLPWICDQRCSSPSSPG RW in isoform 2.
VSP_037526
Natural variant3111A → T.
Corresponds to variant rs7206332 [ dbSNP | Ensembl ].
VAR_057993
Natural variant3181T → M.
Corresponds to variant rs3826107 [ dbSNP | Ensembl ].
VAR_021416

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 16, 2009. Version 3.
Checksum: 97642D54BE926E06

FASTA41146,161
        10         20         30         40         50         60 
MGMRARVPKV AHSTRRPPAA RMWLPRFSSK TVTVLLLAQT TCLLLFIISR PGPSSPAGGE 

        70         80         90        100        110        120 
DRVHVLVLSS WRSGSSFLGQ LFSQHPDVFY LMEPAWHVWT TLSQGSAATL HMAVRDLMRS 

       130        140        150        160        170        180 
IFLCDMDVFD AYMPQSRNLS AFFNWATSRA LCSPPACSAF PRGTISKQDV CKTLCTRQPF 

       190        200        210        220        230        240 
SLAREACRSY SHVVLKEVRF FNLQVLYPLL SDPALNLRIV HLVRDPRAVL RSREAAGPIL 

       250        260        270        280        290        300 
ARDNGIVLGT NGKWVEADPH LRLIREVCRS HVRIAEAATL KPPPFLRGRY RLVRFEDLAR 

       310        320        330        340        350        360 
EPLAEIRALY AFTGLTLTPQ LEAWIHNITH GSGIGKPIEA FHTSSRNARN VSQAWRHALP 

       370        380        390        400        410 
FTKILRVQEV CAGALQLLGY RPVYSADQQR DLTLDLVLPR GPDHFSWASP D 

« Hide

Isoform 2 [UniParc].

Checksum: 29A0083BA75E0EBF
Show »

FASTA41746,894

References

« Hide 'large scale' references
[1]"Cloning and characterization of a mammalian N-acetylglucosamine-6-sulfotransferase that is highly restricted to intestinal tissue."
Lee J.K., Bhakta S., Rosen S.D., Hemmerich S.
Biochem. Biophys. Res. Commun. 263:543-549(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY.
Tissue: Intestine.
[2]"Macular corneal dystrophy type I and type II are caused by distinct mutations in a new sulphotransferase gene."
Akama T.O., Nishida K., Nakayama J., Watanabe H., Ozaki K., Nakamura T., Dota A., Kawasaki S., Inoue Y., Maeda N., Yamamoto S., Fujiwara T., Thonar E.J.-M.A., Shimomura Y., Kinoshita S., Tanigami A., Fukuda M.N.
Nat. Genet. 26:237-241(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[5]"Sulfation of endothelial mucin by corneal keratan N-acetylglucosamine 6-O-sulfotransferase (GST-4beta)."
Bartes A., Bhakta S., Hemmerich S.
Biochem. Biophys. Res. Commun. 282:928-933(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]"Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic mucinous adenocarcinoma."
Seko A., Nagata K., Yonezawa S., Yamashita K.
Glycobiology 12:379-388(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate."
Akama T.O., Misra A.K., Hindsgaul O., Fukuda M.N.
J. Biol. Chem. 277:42505-42513(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBSTRATE SPECIFICITY.
[8]"Activities and expression pattern of the carbohydrate sulfotransferase GlcNAc6ST-3 (I-GlcNAc6ST): functional implications."
Lee J.K., Bistrup A., van Zante A., Rosen S.D.
Glycobiology 13:245-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBSTRATE SPECIFICITY, FUNCTION, TISSUE SPECIFICITY.
[9]"Golgi localization of carbohydrate sulfotransferases is a determinant of L-selectin ligand biosynthesis."
de Graffenried C.L., Bertozzi C.R.
J. Biol. Chem. 278:40282-40295(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF176838 mRNA. Translation: AAD56000.1. Different initiation.
AF176839 Genomic DNA. Translation: AAD56001.1. Different initiation.
AF246718 mRNA. Translation: AAG28023.1.
AF219991 Genomic DNA. Translation: AAG26326.1.
CH471114 Genomic DNA. Translation: EAW95635.1.
BC147002 mRNA. Translation: AAI47003.1.
BC147007 mRNA. Translation: AAI47008.1.
RefSeqNP_078809.2. NM_024533.4.
UniGeneHs.745424.

3D structure databases

ProteinModelPortalQ9GZS9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000338783.

Polymorphism databases

DMDM239938912.

Proteomic databases

PaxDbQ9GZS9.
PRIDEQ9GZS9.

Protocols and materials databases

DNASU23563.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000336257; ENSP00000338783; ENSG00000135702. [Q9GZS9-1]
ENST00000541075; ENSP00000441220; ENSG00000135702. [Q9GZS9-2]
GeneID23563.
KEGGhsa:23563.
UCSCuc002fei.3. human. [Q9GZS9-1]
uc002fej.1. human. [Q9GZS9-2]

Organism-specific databases

CTD23563.
GeneCardsGC16M075562.
H-InvDBHIX0079828.
HGNCHGNC:1973. CHST5.
HPAHPA043804.
MIM604817. gene.
neXtProtNX_Q9GZS9.
PharmGKBPA26505.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG80862.
HOGENOMHOG000261614.
HOVERGENHBG050949.
InParanoidQ9GZS9.
KOK09670.
OMAIREVCRS.
OrthoDBEOG7RZ5S0.
PhylomeDBQ9GZS9.
TreeFamTF342871.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
REACT_116125. Disease.
SABIO-RKQ9GZS9.

Gene expression databases

BgeeQ9GZS9.
CleanExHS_CHST5.
GenevestigatorQ9GZS9.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMSSF52540. SSF52540. 2 hits.
ProtoNetSearch...

Other

GeneWikiCHST5.
GenomeRNAi23563.
NextBio46152.
PROQ9GZS9.
SOURCESearch...

Entry information

Entry nameCHST5_HUMAN
AccessionPrimary (citable) accession number: Q9GZS9
Secondary accession number(s): B2RV23, Q7LCN3, Q9UBY3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 16, 2009
Last modified: April 16, 2014
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM