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Q9GZS9

- CHST5_HUMAN

UniProt

Q9GZS9 - CHST5_HUMAN

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Protein
Carbohydrate sulfotransferase 5
Gene
CHST5
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi71 – 777PAPS By similarity
Nucleotide bindingi224 – 2329PAPS By similarity

GO - Molecular functioni

  1. N-acetylglucosamine 6-O-sulfotransferase activity Source: UniProtKB
  2. sulfotransferase activity Source: ProtInc
Complete GO annotation...

GO - Biological processi

  1. N-acetylglucosamine metabolic process Source: UniProtKB
  2. carbohydrate metabolic process Source: Reactome
  3. glycosaminoglycan metabolic process Source: Reactome
  4. keratan sulfate biosynthetic process Source: Reactome
  5. keratan sulfate metabolic process Source: Reactome
  6. protein sulfation Source: ProtInc
  7. small molecule metabolic process Source: Reactome
  8. sulfur compound metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

ReactomeiREACT_121120. Keratan sulfate biosynthesis.
SABIO-RKQ9GZS9.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 5 (EC:2.8.2.-)
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-alpha
Short name:
GST4-alpha
Intestinal N-acetylglucosamine-6-O-sulfotransferase
Short name:
I-GlcNAc6ST
Short name:
Intestinal GlcNAc-6-sulfotransferase
Short name:
hIGn6ST
N-acetylglucosamine 6-O-sulfotransferase 3
Short name:
GlcNAc6ST-3
Short name:
Gn6st-3
Gene namesi
Name:CHST5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:1973. CHST5.

Subcellular locationi

Golgi apparatus membrane; Single-pass type II membrane protein Inferred
Note: Golgi membrane, early secretory pathway.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3030Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei31 – 4818Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini49 – 411363Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB
  2. Golgi membrane Source: Reactome
  3. integral component of membrane Source: UniProtKB
  4. intrinsic component of Golgi membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26505.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Carbohydrate sulfotransferase 5
PRO_0000085195Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi138 – 1381N-linked (GlcNAc...) Reviewed prediction
Glycosylationi327 – 3271N-linked (GlcNAc...) Reviewed prediction
Glycosylationi350 – 3501N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9GZS9.
PRIDEiQ9GZS9.

Expressioni

Tissue specificityi

Predominantly expressed in small and large intestines and colon. Weakly expressed in lymphocytes. Not expressed in other tissues. Down-regulated in colonic adenocarcinomas.5 Publications

Gene expression databases

BgeeiQ9GZS9.
CleanExiHS_CHST5.
GenevestigatoriQ9GZS9.

Organism-specific databases

HPAiHPA043804.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000338783.

Structurei

3D structure databases

ProteinModelPortaliQ9GZS9.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG80862.
HOGENOMiHOG000261614.
HOVERGENiHBG050949.
InParanoidiQ9GZS9.
KOiK09670.
OMAiIREVCRS.
OrthoDBiEOG7RZ5S0.
PhylomeDBiQ9GZS9.
TreeFamiTF342871.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9GZS9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGMRARVPKV AHSTRRPPAA RMWLPRFSSK TVTVLLLAQT TCLLLFIISR    50
PGPSSPAGGE DRVHVLVLSS WRSGSSFLGQ LFSQHPDVFY LMEPAWHVWT 100
TLSQGSAATL HMAVRDLMRS IFLCDMDVFD AYMPQSRNLS AFFNWATSRA 150
LCSPPACSAF PRGTISKQDV CKTLCTRQPF SLAREACRSY SHVVLKEVRF 200
FNLQVLYPLL SDPALNLRIV HLVRDPRAVL RSREAAGPIL ARDNGIVLGT 250
NGKWVEADPH LRLIREVCRS HVRIAEAATL KPPPFLRGRY RLVRFEDLAR 300
EPLAEIRALY AFTGLTLTPQ LEAWIHNITH GSGIGKPIEA FHTSSRNARN 350
VSQAWRHALP FTKILRVQEV CAGALQLLGY RPVYSADQQR DLTLDLVLPR 400
GPDHFSWASP D 411
Length:411
Mass (Da):46,161
Last modified:June 16, 2009 - v3
Checksum:i97642D54BE926E06
GO
Isoform 2 (identifier: Q9GZS9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: MGMRARVPKVAHSTRR → MSRHLPWICDQRCSSPSSPGRW

Show »
Length:417
Mass (Da):46,894
Checksum:i29A0083BA75E0EBF
GO

Sequence cautioni

The sequence AAD56000.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAD56001.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti311 – 3111A → T.
Corresponds to variant rs7206332 [ dbSNP | Ensembl ].
VAR_057993
Natural varianti318 – 3181T → M.
Corresponds to variant rs3826107 [ dbSNP | Ensembl ].
VAR_021416

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1616MGMRA…HSTRR → MSRHLPWICDQRCSSPSSPG RW in isoform 2.
VSP_037526Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF176838 mRNA. Translation: AAD56000.1. Different initiation.
AF176839 Genomic DNA. Translation: AAD56001.1. Different initiation.
AF246718 mRNA. Translation: AAG28023.1.
AF219991 Genomic DNA. Translation: AAG26326.1.
CH471114 Genomic DNA. Translation: EAW95635.1.
BC147002 mRNA. Translation: AAI47003.1.
BC147007 mRNA. Translation: AAI47008.1.
CCDSiCCDS10919.1. [Q9GZS9-1]
RefSeqiNP_078809.2. NM_024533.4. [Q9GZS9-1]
UniGeneiHs.745424.

Genome annotation databases

EnsembliENST00000336257; ENSP00000338783; ENSG00000135702. [Q9GZS9-1]
ENST00000541075; ENSP00000441220; ENSG00000135702. [Q9GZS9-2]
GeneIDi23563.
KEGGihsa:23563.
UCSCiuc002fei.3. human. [Q9GZS9-1]
uc002fej.1. human. [Q9GZS9-2]

Polymorphism databases

DMDMi239938912.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF176838 mRNA. Translation: AAD56000.1 . Different initiation.
AF176839 Genomic DNA. Translation: AAD56001.1 . Different initiation.
AF246718 mRNA. Translation: AAG28023.1 .
AF219991 Genomic DNA. Translation: AAG26326.1 .
CH471114 Genomic DNA. Translation: EAW95635.1 .
BC147002 mRNA. Translation: AAI47003.1 .
BC147007 mRNA. Translation: AAI47008.1 .
CCDSi CCDS10919.1. [Q9GZS9-1 ]
RefSeqi NP_078809.2. NM_024533.4. [Q9GZS9-1 ]
UniGenei Hs.745424.

3D structure databases

ProteinModelPortali Q9GZS9.
ModBasei Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000338783.

Polymorphism databases

DMDMi 239938912.

Proteomic databases

PaxDbi Q9GZS9.
PRIDEi Q9GZS9.

Protocols and materials databases

DNASUi 23563.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000336257 ; ENSP00000338783 ; ENSG00000135702 . [Q9GZS9-1 ]
ENST00000541075 ; ENSP00000441220 ; ENSG00000135702 . [Q9GZS9-2 ]
GeneIDi 23563.
KEGGi hsa:23563.
UCSCi uc002fei.3. human. [Q9GZS9-1 ]
uc002fej.1. human. [Q9GZS9-2 ]

Organism-specific databases

CTDi 23563.
GeneCardsi GC16M075562.
H-InvDB HIX0079828.
HGNCi HGNC:1973. CHST5.
HPAi HPA043804.
MIMi 604817. gene.
neXtProti NX_Q9GZS9.
PharmGKBi PA26505.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG80862.
HOGENOMi HOG000261614.
HOVERGENi HBG050949.
InParanoidi Q9GZS9.
KOi K09670.
OMAi IREVCRS.
OrthoDBi EOG7RZ5S0.
PhylomeDBi Q9GZS9.
TreeFami TF342871.

Enzyme and pathway databases

Reactomei REACT_121120. Keratan sulfate biosynthesis.
SABIO-RK Q9GZS9.

Miscellaneous databases

GeneWikii CHST5.
GenomeRNAii 23563.
NextBioi 46152.
PROi Q9GZS9.
SOURCEi Search...

Gene expression databases

Bgeei Q9GZS9.
CleanExi HS_CHST5.
Genevestigatori Q9GZS9.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view ]
Pfami PF00685. Sulfotransfer_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMi SSF52540. SSF52540. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a mammalian N-acetylglucosamine-6-sulfotransferase that is highly restricted to intestinal tissue."
    Lee J.K., Bhakta S., Rosen S.D., Hemmerich S.
    Biochem. Biophys. Res. Commun. 263:543-549(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Intestine.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. "Sulfation of endothelial mucin by corneal keratan N-acetylglucosamine 6-O-sulfotransferase (GST-4beta)."
    Bartes A., Bhakta S., Hemmerich S.
    Biochem. Biophys. Res. Commun. 282:928-933(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. "Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic mucinous adenocarcinoma."
    Seko A., Nagata K., Yonezawa S., Yamashita K.
    Glycobiology 12:379-388(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate."
    Akama T.O., Misra A.K., Hindsgaul O., Fukuda M.N.
    J. Biol. Chem. 277:42505-42513(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBSTRATE SPECIFICITY.
  8. "Activities and expression pattern of the carbohydrate sulfotransferase GlcNAc6ST-3 (I-GlcNAc6ST): functional implications."
    Lee J.K., Bistrup A., van Zante A., Rosen S.D.
    Glycobiology 13:245-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBSTRATE SPECIFICITY, FUNCTION, TISSUE SPECIFICITY.
  9. "Golgi localization of carbohydrate sulfotransferases is a determinant of L-selectin ligand biosynthesis."
    de Graffenried C.L., Bertozzi C.R.
    J. Biol. Chem. 278:40282-40295(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCHST5_HUMAN
AccessioniPrimary (citable) accession number: Q9GZS9
Secondary accession number(s): B2RV23, Q7LCN3, Q9UBY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 16, 2009
Last modified: September 3, 2014
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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