Q9GZS9 (CHST5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbohydrate sulfotransferase 5
EC=2.8.2.-

Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-alpha
Short name=GST4-alpha
Intestinal N-acetylglucosamine-6-O-sulfotransferase
Short name=I-GlcNAc6ST
Short name=Intestinal GlcNAc-6-sulfotransferase
Short name=hIGn6ST
N-acetylglucosamine 6-O-sulfotransferase 3
Short name=GlcNAc6ST-3
Short name=Gn6st-3

Gene names

Name:

CHST5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location. Ref.1 Ref.5 Ref.7 Ref.8
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable. Note: Golgi membrane, early secretory pathway. Ref.9
Tissue specificity	Predominantly expressed in small and large intestines and colon. Weakly expressed in lymphocytes. Not expressed in other tissues. Down-regulated in colonic adenocarcinomas. Ref.1 Ref.2 Ref.5 Ref.6 Ref.8
Sequence similarities	Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
Sequence caution	The sequence AAD56000.1 differs from that shown. Reason: Erroneous initiation. The sequence AAD56001.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-acetylglucosamine metabolic process Inferred from direct assay Ref.1Ref.7. Source: UniProtKB carbohydrate metabolic process Traceable author statement. Source: Reactome keratan sulfate biosynthetic process Traceable author statement. Source: Reactome protein sulfation Traceable author statement Ref.1. Source: ProtInc
Cellular_component	integral to membrane Non-traceable author statement Ref.1. Source: UniProtKB intrinsic to Golgi membrane Non-traceable author statement Ref.9. Source: UniProtKB
Molecular_function	N-acetylglucosamine 6-O-sulfotransferase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9GZS9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9GZS9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: MGMRARVPKVAHSTRR → MSRHLPWICDQRCSSPSSPGRW

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Carbohydrate sulfotransferase 5		PRO_0000085195
Regions
	Topological domain	1 – 30	30	Cytoplasmic Potential
	Transmembrane	31 – 48	18	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	49 – 411	363	Lumenal Potential
	Nucleotide binding	71 – 77	7	PAPS By similarity
	Nucleotide binding	224 – 232	9	PAPS By similarity
Amino acid modifications
	Glycosylation	138	1	N-linked (GlcNAc...) Potential
	Glycosylation	327	1	N-linked (GlcNAc...) Potential
	Glycosylation	350	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 16	16	MGMRA…HSTRR → MSRHLPWICDQRCSSPSSPG RW in isoform 2.		VSP_037526
	Natural variant	311	1	A → T. Corresponds to variant rs7206332 [ dbSNP | Ensembl ].		VAR_057993
	Natural variant	318	1	T → M. Corresponds to variant rs3826107 [ dbSNP | Ensembl ].		VAR_021416

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 16, 2009. Version 3. Checksum: 97642D54BE926E06	FASTA	411	46,161
	10 20 30 40 50 60 MGMRARVPKV AHSTRRPPAA RMWLPRFSSK TVTVLLLAQT TCLLLFIISR PGPSSPAGGE 70 80 90 100 110 120 DRVHVLVLSS WRSGSSFLGQ LFSQHPDVFY LMEPAWHVWT TLSQGSAATL HMAVRDLMRS 130 140 150 160 170 180 IFLCDMDVFD AYMPQSRNLS AFFNWATSRA LCSPPACSAF PRGTISKQDV CKTLCTRQPF 190 200 210 220 230 240 SLAREACRSY SHVVLKEVRF FNLQVLYPLL SDPALNLRIV HLVRDPRAVL RSREAAGPIL 250 260 270 280 290 300 ARDNGIVLGT NGKWVEADPH LRLIREVCRS HVRIAEAATL KPPPFLRGRY RLVRFEDLAR 310 320 330 340 350 360 EPLAEIRALY AFTGLTLTPQ LEAWIHNITH GSGIGKPIEA FHTSSRNARN VSQAWRHALP 370 380 390 400 410 FTKILRVQEV CAGALQLLGY RPVYSADQQR DLTLDLVLPR GPDHFSWASP D
	Isoform 2 [UniParc]. Checksum: 29A0083BA75E0EBF	FASTA	417	46,894
	10 20 30 40 50 60 MSRHLPWICD QRCSSPSSPG RWPPAARMWL PRFSSKTVTV LLLAQTTCLL LFIISRPGPS 70 80 90 100 110 120 SPAGGEDRVH VLVLSSWRSG SSFLGQLFSQ HPDVFYLMEP AWHVWTTLSQ GSAATLHMAV 130 140 150 160 170 180 RDLMRSIFLC DMDVFDAYMP QSRNLSAFFN WATSRALCSP PACSAFPRGT ISKQDVCKTL 190 200 210 220 230 240 CTRQPFSLAR EACRSYSHVV LKEVRFFNLQ VLYPLLSDPA LNLRIVHLVR DPRAVLRSRE 250 260 270 280 290 300 AAGPILARDN GIVLGTNGKW VEADPHLRLI REVCRSHVRI AEAATLKPPP FLRGRYRLVR 310 320 330 340 350 360 FEDLAREPLA EIRALYAFTG LTLTPQLEAW IHNITHGSGI GKPIEAFHTS SRNARNVSQA 370 380 390 400 410 WRHALPFTKI LRVQEVCAGA LQLLGYRPVY SADQQRDLTL DLVLPRGPDH FSWASPD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a mammalian N-acetylglucosamine-6-sulfotransferase that is highly restricted to intestinal tissue." Lee J.K., Bhakta S., Rosen S.D., Hemmerich S. Biochem. Biophys. Res. Commun. 263:543-549(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY. Tissue: Intestine.
[2]	"Macular corneal dystrophy type I and type II are caused by distinct mutations in a new sulphotransferase gene." Akama T.O., Nishida K., Nakayama J., Watanabe H., Ozaki K., Nakamura T., Dota A., Kawasaki S., Inoue Y., Maeda N., Yamamoto S., Fujiwara T., Thonar E.J.-M.A., Shimomura Y., Kinoshita S., Tanigami A., Fukuda M.N. Nat. Genet. 26:237-241(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[5]	"Sulfation of endothelial mucin by corneal keratan N-acetylglucosamine 6-O-sulfotransferase (GST-4beta)." Bartes A., Bhakta S., Hemmerich S. Biochem. Biophys. Res. Commun. 282:928-933(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]	"Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic mucinous adenocarcinoma." Seko A., Nagata K., Yonezawa S., Yamashita K. Glycobiology 12:379-388(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate." Akama T.O., Misra A.K., Hindsgaul O., Fukuda M.N. J. Biol. Chem. 277:42505-42513(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBSTRATE SPECIFICITY.
[8]	"Activities and expression pattern of the carbohydrate sulfotransferase GlcNAc6ST-3 (I-GlcNAc6ST): functional implications." Lee J.K., Bistrup A., van Zante A., Rosen S.D. Glycobiology 13:245-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBSTRATE SPECIFICITY, FUNCTION, TISSUE SPECIFICITY.
[9]	"Golgi localization of carbohydrate sulfotransferases is a determinant of L-selectin ligand biosynthesis." de Graffenried C.L., Bertozzi C.R. J. Biol. Chem. 278:40282-40295(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF176838 mRNA. Translation: AAD56000.1. Different initiation. AF176839 Genomic DNA. Translation: AAD56001.1. Different initiation. AF246718 mRNA. Translation: AAG28023.1. AF219991 Genomic DNA. Translation: AAG26326.1. CH471114 Genomic DNA. Translation: EAW95635.1. BC147002 mRNA. Translation: AAI47003.1. BC147007 mRNA. Translation: AAI47008.1.
IPI	IPI00554552. IPI00644538.
RefSeq	NP_078809.2. NM_024533.4.
UniGene	Hs.710689.
3D structure databases
ProteinModelPortal	Q9GZS9.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000338783.
Polymorphism databases
DMDM	239938912.
Proteomic databases
PaxDb	Q9GZS9.
PRIDE	Q9GZS9.
Protocols and materials databases
DNASU	23563.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000336257; ENSP00000338783; ENSG00000135702. ENST00000541075; ENSP00000441220; ENSG00000135702.
GeneID	23563.
KEGG	hsa:23563.
UCSC	uc002fei.3. human. uc002fej.1. human.
Organism-specific databases
CTD	23563.
GeneCards	GC16M075562.
H-InvDB	HIX0079828.
HGNC	HGNC:1973. CHST5.
HPA	HPA043804.
MIM	604817. gene.
neXtProt	NX_Q9GZS9.
PharmGKB	PA26505.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG80862.
HOGENOM	HOG000261614.
HOVERGEN	HBG050949.
InParanoid	Q9GZS9.
KO	K09670.
OMA	IREVCRS.
OrthoDB	EOG4BRWKR.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_116125. Disease.
SABIO-RK	Q9GZS9.
Gene expression databases
ArrayExpress	Q9GZS9.
Bgee	Q9GZS9.
CleanEx	HS_CHST5.
Genevestigator	Q9GZS9.
GermOnline	ENSG00000135702. Homo sapiens.
Family and domain databases
InterPro	IPR016469. Carbohydrate_sulfotransferase. IPR000863. Sulfotransferase_dom. [Graphical view]
Pfam	PF00685. Sulfotransfer_1. 1 hit. [Graphical view]
PIRSF	PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	23563.
NextBio	46152.
SOURCE	Search...

Entry information

Entry name

CHST5_HUMAN

Accession

Primary (citable) accession number: Q9GZS9
Secondary accession number(s): B2RV23, Q7LCN3, Q9UBY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 16, 2009
Last modified:	April 3, 2013
This is version 89 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.