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Q9GZS9

- CHST5_HUMAN

UniProt

Q9GZS9 - CHST5_HUMAN

Protein

Carbohydrate sulfotransferase 5

Gene

CHST5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 3 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location.4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi71 – 777PAPSBy similarity
    Nucleotide bindingi224 – 2329PAPSBy similarity

    GO - Molecular functioni

    1. N-acetylglucosamine 6-O-sulfotransferase activity Source: UniProtKB
    2. sulfotransferase activity Source: ProtInc

    GO - Biological processi

    1. carbohydrate metabolic process Source: Reactome
    2. glycosaminoglycan metabolic process Source: Reactome
    3. keratan sulfate biosynthetic process Source: Reactome
    4. keratan sulfate metabolic process Source: Reactome
    5. N-acetylglucosamine metabolic process Source: UniProtKB
    6. protein sulfation Source: ProtInc
    7. small molecule metabolic process Source: Reactome
    8. sulfur compound metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    ReactomeiREACT_121120. Keratan sulfate biosynthesis.
    SABIO-RKQ9GZS9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbohydrate sulfotransferase 5 (EC:2.8.2.-)
    Alternative name(s):
    Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-alpha
    Short name:
    GST4-alpha
    Intestinal N-acetylglucosamine-6-O-sulfotransferase
    Short name:
    I-GlcNAc6ST
    Short name:
    Intestinal GlcNAc-6-sulfotransferase
    Short name:
    hIGn6ST
    N-acetylglucosamine 6-O-sulfotransferase 3
    Short name:
    GlcNAc6ST-3
    Short name:
    Gn6st-3
    Gene namesi
    Name:CHST5
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:1973. CHST5.

    Subcellular locationi

    Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein 1 Publication
    Note: Golgi membrane, early secretory pathway.

    GO - Cellular componenti

    1. Golgi apparatus Source: UniProtKB
    2. Golgi membrane Source: Reactome
    3. integral component of membrane Source: UniProtKB
    4. intrinsic component of Golgi membrane Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26505.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 411411Carbohydrate sulfotransferase 5PRO_0000085195Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi327 – 3271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi350 – 3501N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9GZS9.
    PRIDEiQ9GZS9.

    Expressioni

    Tissue specificityi

    Predominantly expressed in small and large intestines and colon. Weakly expressed in lymphocytes. Not expressed in other tissues. Down-regulated in colonic adenocarcinomas.5 Publications

    Gene expression databases

    BgeeiQ9GZS9.
    CleanExiHS_CHST5.
    GenevestigatoriQ9GZS9.

    Organism-specific databases

    HPAiHPA043804.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000338783.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9GZS9.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3030CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini49 – 411363LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei31 – 4818Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG80862.
    HOGENOMiHOG000261614.
    HOVERGENiHBG050949.
    InParanoidiQ9GZS9.
    KOiK09670.
    OMAiIREVCRS.
    OrthoDBiEOG7RZ5S0.
    PhylomeDBiQ9GZS9.
    TreeFamiTF342871.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR016469. Carbohydrate_sulfotransferase.
    IPR027417. P-loop_NTPase.
    IPR000863. Sulfotransferase_dom.
    [Graphical view]
    PfamiPF00685. Sulfotransfer_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
    SUPFAMiSSF52540. SSF52540. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9GZS9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGMRARVPKV AHSTRRPPAA RMWLPRFSSK TVTVLLLAQT TCLLLFIISR    50
    PGPSSPAGGE DRVHVLVLSS WRSGSSFLGQ LFSQHPDVFY LMEPAWHVWT 100
    TLSQGSAATL HMAVRDLMRS IFLCDMDVFD AYMPQSRNLS AFFNWATSRA 150
    LCSPPACSAF PRGTISKQDV CKTLCTRQPF SLAREACRSY SHVVLKEVRF 200
    FNLQVLYPLL SDPALNLRIV HLVRDPRAVL RSREAAGPIL ARDNGIVLGT 250
    NGKWVEADPH LRLIREVCRS HVRIAEAATL KPPPFLRGRY RLVRFEDLAR 300
    EPLAEIRALY AFTGLTLTPQ LEAWIHNITH GSGIGKPIEA FHTSSRNARN 350
    VSQAWRHALP FTKILRVQEV CAGALQLLGY RPVYSADQQR DLTLDLVLPR 400
    GPDHFSWASP D 411
    Length:411
    Mass (Da):46,161
    Last modified:June 16, 2009 - v3
    Checksum:i97642D54BE926E06
    GO
    Isoform 2 (identifier: Q9GZS9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-16: MGMRARVPKVAHSTRR → MSRHLPWICDQRCSSPSSPGRW

    Show »
    Length:417
    Mass (Da):46,894
    Checksum:i29A0083BA75E0EBF
    GO

    Sequence cautioni

    The sequence AAD56000.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAD56001.1 differs from that shown. Reason: Erroneous initiation.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti311 – 3111A → T.
    Corresponds to variant rs7206332 [ dbSNP | Ensembl ].
    VAR_057993
    Natural varianti318 – 3181T → M.
    Corresponds to variant rs3826107 [ dbSNP | Ensembl ].
    VAR_021416

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1616MGMRA…HSTRR → MSRHLPWICDQRCSSPSSPG RW in isoform 2. 1 PublicationVSP_037526Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF176838 mRNA. Translation: AAD56000.1. Different initiation.
    AF176839 Genomic DNA. Translation: AAD56001.1. Different initiation.
    AF246718 mRNA. Translation: AAG28023.1.
    AF219991 Genomic DNA. Translation: AAG26326.1.
    CH471114 Genomic DNA. Translation: EAW95635.1.
    BC147002 mRNA. Translation: AAI47003.1.
    BC147007 mRNA. Translation: AAI47008.1.
    CCDSiCCDS10919.1. [Q9GZS9-1]
    RefSeqiNP_078809.2. NM_024533.4. [Q9GZS9-1]
    UniGeneiHs.745424.

    Genome annotation databases

    EnsembliENST00000336257; ENSP00000338783; ENSG00000135702. [Q9GZS9-1]
    GeneIDi23563.
    KEGGihsa:23563.
    UCSCiuc002fei.3. human. [Q9GZS9-1]
    uc002fej.1. human. [Q9GZS9-2]

    Polymorphism databases

    DMDMi239938912.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF176838 mRNA. Translation: AAD56000.1 . Different initiation.
    AF176839 Genomic DNA. Translation: AAD56001.1 . Different initiation.
    AF246718 mRNA. Translation: AAG28023.1 .
    AF219991 Genomic DNA. Translation: AAG26326.1 .
    CH471114 Genomic DNA. Translation: EAW95635.1 .
    BC147002 mRNA. Translation: AAI47003.1 .
    BC147007 mRNA. Translation: AAI47008.1 .
    CCDSi CCDS10919.1. [Q9GZS9-1 ]
    RefSeqi NP_078809.2. NM_024533.4. [Q9GZS9-1 ]
    UniGenei Hs.745424.

    3D structure databases

    ProteinModelPortali Q9GZS9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000338783.

    Polymorphism databases

    DMDMi 239938912.

    Proteomic databases

    PaxDbi Q9GZS9.
    PRIDEi Q9GZS9.

    Protocols and materials databases

    DNASUi 23563.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000336257 ; ENSP00000338783 ; ENSG00000135702 . [Q9GZS9-1 ]
    GeneIDi 23563.
    KEGGi hsa:23563.
    UCSCi uc002fei.3. human. [Q9GZS9-1 ]
    uc002fej.1. human. [Q9GZS9-2 ]

    Organism-specific databases

    CTDi 23563.
    GeneCardsi GC16M075562.
    H-InvDB HIX0079828.
    HGNCi HGNC:1973. CHST5.
    HPAi HPA043804.
    MIMi 604817. gene.
    neXtProti NX_Q9GZS9.
    PharmGKBi PA26505.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG80862.
    HOGENOMi HOG000261614.
    HOVERGENi HBG050949.
    InParanoidi Q9GZS9.
    KOi K09670.
    OMAi IREVCRS.
    OrthoDBi EOG7RZ5S0.
    PhylomeDBi Q9GZS9.
    TreeFami TF342871.

    Enzyme and pathway databases

    Reactomei REACT_121120. Keratan sulfate biosynthesis.
    SABIO-RK Q9GZS9.

    Miscellaneous databases

    GeneWikii CHST5.
    GenomeRNAii 23563.
    NextBioi 46152.
    PROi Q9GZS9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9GZS9.
    CleanExi HS_CHST5.
    Genevestigatori Q9GZS9.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR016469. Carbohydrate_sulfotransferase.
    IPR027417. P-loop_NTPase.
    IPR000863. Sulfotransferase_dom.
    [Graphical view ]
    Pfami PF00685. Sulfotransfer_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
    SUPFAMi SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a mammalian N-acetylglucosamine-6-sulfotransferase that is highly restricted to intestinal tissue."
      Lee J.K., Bhakta S., Rosen S.D., Hemmerich S.
      Biochem. Biophys. Res. Commun. 263:543-549(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY.
      Tissue: Intestine.
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    5. "Sulfation of endothelial mucin by corneal keratan N-acetylglucosamine 6-O-sulfotransferase (GST-4beta)."
      Bartes A., Bhakta S., Hemmerich S.
      Biochem. Biophys. Res. Commun. 282:928-933(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    6. "Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic mucinous adenocarcinoma."
      Seko A., Nagata K., Yonezawa S., Yamashita K.
      Glycobiology 12:379-388(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate."
      Akama T.O., Misra A.K., Hindsgaul O., Fukuda M.N.
      J. Biol. Chem. 277:42505-42513(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBSTRATE SPECIFICITY.
    8. "Activities and expression pattern of the carbohydrate sulfotransferase GlcNAc6ST-3 (I-GlcNAc6ST): functional implications."
      Lee J.K., Bistrup A., van Zante A., Rosen S.D.
      Glycobiology 13:245-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBSTRATE SPECIFICITY, FUNCTION, TISSUE SPECIFICITY.
    9. "Golgi localization of carbohydrate sulfotransferases is a determinant of L-selectin ligand biosynthesis."
      de Graffenried C.L., Bertozzi C.R.
      J. Biol. Chem. 278:40282-40295(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCHST5_HUMAN
    AccessioniPrimary (citable) accession number: Q9GZS9
    Secondary accession number(s): B2RV23, Q7LCN3, Q9UBY3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 101 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3