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Protein

Carbohydrate sulfotransferase 5

Gene

CHST5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi71 – 77PAPSBy similarity7
Nucleotide bindingi224 – 232PAPSBy similarity9

GO - Molecular functioni

  • N-acetylglucosamine 6-O-sulfotransferase activity Source: UniProtKB
  • sulfotransferase activity Source: ProtInc

GO - Biological processi

  • carbohydrate metabolic process Source: UniProtKB-KW
  • keratan sulfate biosynthetic process Source: GO_Central
  • N-acetylglucosamine metabolic process Source: UniProtKB
  • protein sulfation Source: ProtInc
  • sulfur compound metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

ReactomeiR-HSA-2022854. Keratan sulfate biosynthesis.
SABIO-RKQ9GZS9.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 5 (EC:2.8.2.-)
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-alpha
Short name:
GST4-alpha
Intestinal N-acetylglucosamine-6-O-sulfotransferase
Short name:
I-GlcNAc6ST
Short name:
Intestinal GlcNAc-6-sulfotransferase
Short name:
hIGn6ST
N-acetylglucosamine 6-O-sulfotransferase 3
Short name:
GlcNAc6ST-3
Short name:
Gn6st-3
Gene namesi
Name:CHST5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:1973. CHST5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30CytoplasmicSequence analysisAdd BLAST30
Transmembranei31 – 48Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST18
Topological domaini49 – 411LumenalSequence analysisAdd BLAST363

GO - Cellular componenti

  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: Reactome
  • integral component of membrane Source: UniProtKB
  • intrinsic component of Golgi membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi23563.
OpenTargetsiENSG00000135702.
PharmGKBiPA26505.

Polymorphism and mutation databases

BioMutaiCHST5.
DMDMi239938912.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000851951 – 411Carbohydrate sulfotransferase 5Add BLAST411

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi138N-linked (GlcNAc...)Sequence analysis1
Glycosylationi327N-linked (GlcNAc...)Sequence analysis1
Glycosylationi350N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9GZS9.
PeptideAtlasiQ9GZS9.
PRIDEiQ9GZS9.

PTM databases

iPTMnetiQ9GZS9.
PhosphoSitePlusiQ9GZS9.

Expressioni

Tissue specificityi

Predominantly expressed in small and large intestines and colon. Weakly expressed in lymphocytes. Not expressed in other tissues. Down-regulated in colonic adenocarcinomas.5 Publications

Gene expression databases

BgeeiENSG00000135702.
CleanExiHS_CHST5.
ExpressionAtlasiQ9GZS9. baseline and differential.
GenevisibleiQ9GZS9. HS.

Organism-specific databases

HPAiHPA043804.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000338783.

Structurei

3D structure databases

ProteinModelPortaliQ9GZS9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJXD. Eukaryota.
ENOG4110V0B. LUCA.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG050949.
InParanoidiQ9GZS9.
KOiK09670.
OMAiLRVQEVC.
OrthoDBiEOG091G0V3Y.
PhylomeDBiQ9GZS9.
TreeFamiTF342871.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9GZS9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGMRARVPKV AHSTRRPPAA RMWLPRFSSK TVTVLLLAQT TCLLLFIISR
60 70 80 90 100
PGPSSPAGGE DRVHVLVLSS WRSGSSFLGQ LFSQHPDVFY LMEPAWHVWT
110 120 130 140 150
TLSQGSAATL HMAVRDLMRS IFLCDMDVFD AYMPQSRNLS AFFNWATSRA
160 170 180 190 200
LCSPPACSAF PRGTISKQDV CKTLCTRQPF SLAREACRSY SHVVLKEVRF
210 220 230 240 250
FNLQVLYPLL SDPALNLRIV HLVRDPRAVL RSREAAGPIL ARDNGIVLGT
260 270 280 290 300
NGKWVEADPH LRLIREVCRS HVRIAEAATL KPPPFLRGRY RLVRFEDLAR
310 320 330 340 350
EPLAEIRALY AFTGLTLTPQ LEAWIHNITH GSGIGKPIEA FHTSSRNARN
360 370 380 390 400
VSQAWRHALP FTKILRVQEV CAGALQLLGY RPVYSADQQR DLTLDLVLPR
410
GPDHFSWASP D
Length:411
Mass (Da):46,161
Last modified:June 16, 2009 - v3
Checksum:i97642D54BE926E06
GO
Isoform 2 (identifier: Q9GZS9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: MGMRARVPKVAHSTRR → MSRHLPWICDQRCSSPSSPGRW

Show »
Length:417
Mass (Da):46,894
Checksum:i29A0083BA75E0EBF
GO

Sequence cautioni

The sequence AAD56000 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAD56001 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_057993311A → T.Corresponds to variant rs7206332dbSNPEnsembl.1
Natural variantiVAR_021416318T → M.Corresponds to variant rs3826107dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0375261 – 16MGMRA…HSTRR → MSRHLPWICDQRCSSPSSPG RW in isoform 2. 1 PublicationAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176838 mRNA. Translation: AAD56000.1. Different initiation.
AF176839 Genomic DNA. Translation: AAD56001.1. Different initiation.
AF246718 mRNA. Translation: AAG28023.1.
AF219991 Genomic DNA. Translation: AAG26326.1.
CH471114 Genomic DNA. Translation: EAW95635.1.
BC147002 mRNA. Translation: AAI47003.1.
BC147007 mRNA. Translation: AAI47008.1.
CCDSiCCDS10919.1. [Q9GZS9-1]
RefSeqiNP_078809.2. NM_024533.4. [Q9GZS9-1]
UniGeneiHs.745424.

Genome annotation databases

EnsembliENST00000336257; ENSP00000338783; ENSG00000135702. [Q9GZS9-1]
GeneIDi23563.
KEGGihsa:23563.
UCSCiuc002fei.4. human. [Q9GZS9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176838 mRNA. Translation: AAD56000.1. Different initiation.
AF176839 Genomic DNA. Translation: AAD56001.1. Different initiation.
AF246718 mRNA. Translation: AAG28023.1.
AF219991 Genomic DNA. Translation: AAG26326.1.
CH471114 Genomic DNA. Translation: EAW95635.1.
BC147002 mRNA. Translation: AAI47003.1.
BC147007 mRNA. Translation: AAI47008.1.
CCDSiCCDS10919.1. [Q9GZS9-1]
RefSeqiNP_078809.2. NM_024533.4. [Q9GZS9-1]
UniGeneiHs.745424.

3D structure databases

ProteinModelPortaliQ9GZS9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000338783.

PTM databases

iPTMnetiQ9GZS9.
PhosphoSitePlusiQ9GZS9.

Polymorphism and mutation databases

BioMutaiCHST5.
DMDMi239938912.

Proteomic databases

PaxDbiQ9GZS9.
PeptideAtlasiQ9GZS9.
PRIDEiQ9GZS9.

Protocols and materials databases

DNASUi23563.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336257; ENSP00000338783; ENSG00000135702. [Q9GZS9-1]
GeneIDi23563.
KEGGihsa:23563.
UCSCiuc002fei.4. human. [Q9GZS9-1]

Organism-specific databases

CTDi23563.
DisGeNETi23563.
GeneCardsiCHST5.
H-InvDBHIX0079828.
HGNCiHGNC:1973. CHST5.
HPAiHPA043804.
MIMi604817. gene.
neXtProtiNX_Q9GZS9.
OpenTargetsiENSG00000135702.
PharmGKBiPA26505.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJXD. Eukaryota.
ENOG4110V0B. LUCA.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG050949.
InParanoidiQ9GZS9.
KOiK09670.
OMAiLRVQEVC.
OrthoDBiEOG091G0V3Y.
PhylomeDBiQ9GZS9.
TreeFamiTF342871.

Enzyme and pathway databases

ReactomeiR-HSA-2022854. Keratan sulfate biosynthesis.
SABIO-RKQ9GZS9.

Miscellaneous databases

ChiTaRSiCHST5. human.
GeneWikiiCHST5.
GenomeRNAii23563.
PROiQ9GZS9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000135702.
CleanExiHS_CHST5.
ExpressionAtlasiQ9GZS9. baseline and differential.
GenevisibleiQ9GZS9. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCHST5_HUMAN
AccessioniPrimary (citable) accession number: Q9GZS9
Secondary accession number(s): B2RV23, Q7LCN3, Q9UBY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.