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Protein

WD repeat-containing protein 61

Gene

WDR61

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.4 Publications

GO - Biological processi

  • histone H3-K4 trimethylation Source: UniProtKB
  • negative regulation of myeloid cell differentiation Source: UniProtKB
  • positive regulation of histone H3-K4 methylation Source: UniProtKB
  • positive regulation of histone H3-K79 methylation Source: UniProtKB
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000140395-MONOMER.
ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-112387. Elongation arrest and recovery.
R-HSA-429958. mRNA decay by 3' to 5' exoribonuclease.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-75955. RNA Polymerase II Transcription Elongation.
SignaLinkiQ9GZS3.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein 61
Alternative name(s):
Meiotic recombination REC14 protein homolog
SKI8 homolog
Short name:
Ski8
Cleaved into the following chain:
Gene namesi
Name:WDR61
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:30300. WDR61.

Subcellular locationi

GO - Cellular componenti

  • Cdc73/Paf1 complex Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • Ski complex Source: UniProtKB
  • transcriptionally active chromatin Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000140395.
PharmGKBiPA142670595.

Polymorphism and mutation databases

BioMutaiWDR61.
DMDMi74761365.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004257481 – 305WD repeat-containing protein 61Add BLAST305
Initiator methionineiRemoved; alternateCombined sources
ChainiPRO_00002458512 – 305WD repeat-containing protein 61, N-terminally processedAdd BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei2N-acetylthreonine; in WD repeat-containing protein 61, N-terminally processedCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9GZS3.
MaxQBiQ9GZS3.
PaxDbiQ9GZS3.
PeptideAtlasiQ9GZS3.
PRIDEiQ9GZS3.

PTM databases

iPTMnetiQ9GZS3.
PhosphoSitePlusiQ9GZS3.

Expressioni

Gene expression databases

BgeeiENSG00000140395.
CleanExiHS_WDR61.
ExpressionAtlasiQ9GZS3. baseline and differential.
GenevisibleiQ9GZS3. HS.

Organism-specific databases

HPAiHPA039932.
HPA040065.

Interactioni

Subunit structurei

Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61. Component of the SKI complex which consists of WDR61, SKIV2L and TTC37.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CTR9Q6PD6210EBI-358545,EBI-1019583

Protein-protein interaction databases

BioGridi123255. 46 interactors.
DIPiDIP-36672N.
IntActiQ9GZS3. 27 interactors.
MINTiMINT-1376321.
STRINGi9606.ENSP00000267973.

Structurei

Secondary structure

1305
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 11Combined sources6
Beta strandi14 – 17Combined sources4
Beta strandi19 – 24Combined sources6
Turni28 – 30Combined sources3
Beta strandi34 – 39Combined sources6
Beta strandi44 – 50Combined sources7
Beta strandi53 – 60Combined sources8
Beta strandi67 – 72Combined sources6
Beta strandi74 – 83Combined sources10
Beta strandi86 – 92Combined sources7
Turni93 – 96Combined sources4
Beta strandi97 – 103Combined sources7
Beta strandi112 – 114Combined sources3
Beta strandi118 – 124Combined sources7
Beta strandi128 – 134Combined sources7
Turni135 – 137Combined sources3
Beta strandi139 – 145Combined sources7
Beta strandi147 – 149Combined sources3
Beta strandi151 – 156Combined sources6
Beta strandi160 – 167Combined sources8
Beta strandi172 – 176Combined sources5
Turni177 – 179Combined sources3
Beta strandi182 – 186Combined sources5
Beta strandi195 – 198Combined sources4
Beta strandi204 – 208Combined sources5
Beta strandi214 – 218Combined sources5
Turni219 – 221Combined sources3
Beta strandi224 – 228Combined sources5
Beta strandi235 – 240Combined sources6
Beta strandi244 – 251Combined sources8
Beta strandi256 – 260Combined sources5
Turni261 – 264Combined sources4
Beta strandi265 – 270Combined sources6
Beta strandi277 – 282Combined sources6
Beta strandi286 – 293Combined sources8
Beta strandi298 – 302Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OW8X-ray2.30A/B/C/D2-305[»]
ProteinModelPortaliQ9GZS3.
SMRiQ9GZS3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9GZS3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati14 – 57WD 1Add BLAST44
Repeati62 – 101WD 2Add BLAST40
Repeati104 – 143WD 3Add BLAST40
Repeati146 – 187WD 4Add BLAST42
Repeati188 – 227WD 5Add BLAST40
Repeati230 – 269WD 6Add BLAST40
Repeati272 – 305WD 7Add BLAST34

Sequence similaritiesi

Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG4155. Eukaryota.
ENOG410XP1Z. LUCA.
GeneTreeiENSGT00810000125363.
HOGENOMiHOG000036765.
HOVERGENiHBG059813.
InParanoidiQ9GZS3.
KOiK12602.
OMAiIATGSHH.
OrthoDBiEOG091G0F6P.
PhylomeDBiQ9GZS3.
TreeFamiTF324549.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9GZS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNQYGILFK QEQAHDDAIW SVAWGTNKKE NSETVVTGSL DDLVKVWKWR
60 70 80 90 100
DERLDLQWSL EGHQLGVVSV DISHTLPIAA SSSLDAHIRL WDLENGKQIK
110 120 130 140 150
SIDAGPVDAW TLAFSPDSQY LATGTHVGKV NIFGVESGKK EYSLDTRGKF
160 170 180 190 200
ILSIAYSPDG KYLASGAIDG IINIFDIATG KLLHTLEGHA MPIRSLTFSP
210 220 230 240 250
DSQLLVTASD DGYIKIYDVQ HANLAGTLSG HASWVLNVAF CPDDTHFVSS
260 270 280 290 300
SSDKSVKVWD VGTRTCVHTF FDHQDQVWGV KYNGNGSKIV SVGDDQEIHI

YDCPI
Length:305
Mass (Da):33,581
Last modified:March 1, 2001 - v1
Checksum:iA26C4BBD4F8ADB80
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91W → R in AAP97249 (Ref. 2) Curated1
Sequence conflicti176D → A in AAP97249 (Ref. 2) Curated1
Sequence conflicti229S → G in CAG33614 (Ref. 4) Curated1
Sequence conflicti255S → N in CAG33614 (Ref. 4) Curated1
Sequence conflicti259W → R in AAP97249 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF309553 mRNA. Translation: AAG31639.1.
AF100786 mRNA. Translation: AAP97225.1.
AF127799 mRNA. Translation: AAP97249.1.
AK024754 mRNA. Translation: BAB14986.1.
CR457333 mRNA. Translation: CAG33614.1.
CH471136 Genomic DNA. Translation: EAW99174.1.
CH471136 Genomic DNA. Translation: EAW99175.1.
BC010080 mRNA. Translation: AAH10080.1.
CCDSiCCDS10300.1.
RefSeqiNP_001290176.1. NM_001303247.1.
NP_001290177.1. NM_001303248.1.
NP_079510.1. NM_025234.2.
XP_011520396.1. XM_011522094.1.
UniGeneiHs.513055.

Genome annotation databases

EnsembliENST00000267973; ENSP00000267973; ENSG00000140395.
ENST00000558311; ENSP00000453801; ENSG00000140395.
GeneIDi80349.
KEGGihsa:80349.
UCSCiuc002bdn.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF309553 mRNA. Translation: AAG31639.1.
AF100786 mRNA. Translation: AAP97225.1.
AF127799 mRNA. Translation: AAP97249.1.
AK024754 mRNA. Translation: BAB14986.1.
CR457333 mRNA. Translation: CAG33614.1.
CH471136 Genomic DNA. Translation: EAW99174.1.
CH471136 Genomic DNA. Translation: EAW99175.1.
BC010080 mRNA. Translation: AAH10080.1.
CCDSiCCDS10300.1.
RefSeqiNP_001290176.1. NM_001303247.1.
NP_001290177.1. NM_001303248.1.
NP_079510.1. NM_025234.2.
XP_011520396.1. XM_011522094.1.
UniGeneiHs.513055.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OW8X-ray2.30A/B/C/D2-305[»]
ProteinModelPortaliQ9GZS3.
SMRiQ9GZS3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123255. 46 interactors.
DIPiDIP-36672N.
IntActiQ9GZS3. 27 interactors.
MINTiMINT-1376321.
STRINGi9606.ENSP00000267973.

PTM databases

iPTMnetiQ9GZS3.
PhosphoSitePlusiQ9GZS3.

Polymorphism and mutation databases

BioMutaiWDR61.
DMDMi74761365.

Proteomic databases

EPDiQ9GZS3.
MaxQBiQ9GZS3.
PaxDbiQ9GZS3.
PeptideAtlasiQ9GZS3.
PRIDEiQ9GZS3.

Protocols and materials databases

DNASUi80349.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000267973; ENSP00000267973; ENSG00000140395.
ENST00000558311; ENSP00000453801; ENSG00000140395.
GeneIDi80349.
KEGGihsa:80349.
UCSCiuc002bdn.4. human.

Organism-specific databases

CTDi80349.
GeneCardsiWDR61.
HGNCiHGNC:30300. WDR61.
HPAiHPA039932.
HPA040065.
MIMi609540. gene.
neXtProtiNX_Q9GZS3.
OpenTargetsiENSG00000140395.
PharmGKBiPA142670595.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4155. Eukaryota.
ENOG410XP1Z. LUCA.
GeneTreeiENSGT00810000125363.
HOGENOMiHOG000036765.
HOVERGENiHBG059813.
InParanoidiQ9GZS3.
KOiK12602.
OMAiIATGSHH.
OrthoDBiEOG091G0F6P.
PhylomeDBiQ9GZS3.
TreeFamiTF324549.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000140395-MONOMER.
ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-112387. Elongation arrest and recovery.
R-HSA-429958. mRNA decay by 3' to 5' exoribonuclease.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-75955. RNA Polymerase II Transcription Elongation.
SignaLinkiQ9GZS3.

Miscellaneous databases

ChiTaRSiWDR61. human.
EvolutionaryTraceiQ9GZS3.
GenomeRNAii80349.
PROiQ9GZS3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000140395.
CleanExiHS_WDR61.
ExpressionAtlasiQ9GZS3. baseline and differential.
GenevisibleiQ9GZS3. HS.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWDR61_HUMAN
AccessioniPrimary (citable) accession number: Q9GZS3
Secondary accession number(s): D3DW84, Q6IA22, Q7Z4X4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.