Q9GZR7 (DDX24_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent RNA helicase DDX24
EC=3.6.4.13

Alternative name(s):
DEAD box protein 24

Gene names

Name:

DDX24

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	ATP-dependent RNA helicase Potential.
Catalytic activity	ATP + H2O = ADP + phosphate.
Tissue specificity	Ubiquitous. Most abundant in heart and brain, but with lowest levels in thymus and small intestine.
Sequence similarities	Belongs to the DEAD box helicase family. DDX24/MAK5 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA metabolic process Non-traceable author statement Ref.1. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA nucleolus Inferred from direct assay. Source: LIFEdb
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent RNA helicase activity Non-traceable author statement. Source: UniProtKB RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 859	859	ATP-dependent RNA helicase DDX24		PRO_0000055029
Regions
	Domain	224 – 528	305	Helicase ATP-binding
	Domain	578 – 723	146	Helicase C-terminal
	Nucleotide binding	237 – 244	8	ATP By similarity
	Motif	192 – 220	29	Q motif
	Motif	471 – 474	4	DEAD box
	Compositional bias	83 – 89	7	Poly-Glu
	Compositional bias	153 – 161	9	Poly-Lys
	Compositional bias	835 – 845	11	Poly-Lys
Amino acid modifications
	Modified residue	17	1	N6-acetyllysine Ref.10
	Modified residue	71	1	N6-acetyllysine Ref.10
	Modified residue	82	1	Phosphoserine Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.11 Ref.13
	Modified residue	94	1	Phosphoserine Ref.7 Ref.9
	Modified residue	287	1	Phosphoserine Ref.7 Ref.9
	Modified residue	295	1	Phosphoserine Ref.7 Ref.9
	Modified residue	302	1	Phosphothreonine Ref.7
Natural variations
	Natural variant	316	1	E → K. Corresponds to variant rs35413935 [ dbSNP | Ensembl ].		VAR_052162

Sequences

	Sequence		Length	Mass (Da)
	Q9GZR7 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: B01B1170B2CFA0F7	FASTA	859	96,332
	10 20 30 40 50 60 MKLKDTKSRP KQSSCGKFQT KGIKVVGKWK EVKIDPNMFA DGQMDDLVCF EELTDYQLVS 70 80 90 100 110 120 PAKNPSSLFS KEAPKRKAQA VSEEEEEEEG KSSSPKKKIK LKKSKNVATE GTSTQKEFEV 130 140 150 160 170 180 KDPELEAQGD DMVCDDPEAG EMTSENLVQT APKKKKNKGK KGLEPSQSTA AKVPKKAKTW 190 200 210 220 230 240 IPEVHDQKAD VSAWKDLFVP RPVLRALSFL GFSAPTPIQA LTLAPAIRDK LDILGAAETG 250 260 270 280 290 300 SGKTLAFAIP MIHAVLQWQK RNAAPPPSNT EAPPGETRTE AGAETRSPGK AEAESDALPD 310 320 330 340 350 360 DTVIESEALP SDIAAEARAK TGGTVSDQAL LFGDDDAGEG PSSLIREKPV PKQNENEEEN 370 380 390 400 410 420 LDKEQTGNLK QELDDKSATC KAYPKRPLLG LVLTPTRELA VQVKQHIDAV ARFTGIKTAI 430 440 450 460 470 480 LVGGMSTQKQ QRMLNRRPEI VVATPGRLWE LIKEKHYHLR NLRQLRCLVV DEADRMVEKG 490 500 510 520 530 540 HFAELSQLLE MLNDSQYNPK RQTLVFSATL TLVHQAPARI LHKKHTKKMD KTAKLDLLMQ 550 560 570 580 590 600 KIGMRGKPKV IDLTRNEATV ETLTETKIHC ETDEKDFYLY YFLMQYPGRS LVFANSISCI 610 620 630 640 650 660 KRLSGLLKVL DIMPLTLHAC MHQKQRLRNL EQFARLEDCV LLATDVAARG LDIPKVQHVI 670 680 690 700 710 720 HYQVPRTSEI YVHRSGRTAR ATNEGLSLML IGPEDVINFK KIYKTLKKDE DIPLFPVQTK 730 740 750 760 770 780 YMDVVKERIR LARQIEKSEY RNFQACLHNS WIEQAAAALE IELEEDMYKG GKADQQEERR 790 800 810 820 830 840 RQKQMKVLKK ELRHLLSQPL FTESQKTKYP TQSGKPPLLV SAPSKSESAL SCLSKQKKKK 850 TKKPKEPQPE QPQPSTSAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of human DDX24 and mouse Ddx24, two novel putative DEAD-box proteins, and mapping DDX24 to human chromosome 14q32." Zhao Y., Yu L., Fu Q., Chen W., Jiang J., Gao J., Zhao S. Genomics 67:351-355(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82; SER-94; SER-287; SER-295 AND THR-302, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82, MASS SPECTROMETRY. Tissue: Liver.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82; SER-94; SER-287 AND SER-295, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-17 AND LYS-71, MASS SPECTROMETRY.
[11]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF214731 mRNA. Translation: AAG02169.1. AL136886 mRNA. Translation: CAB66820.1. AK025162 mRNA. Translation: BAB15079.1. BC008847 mRNA. Translation: AAH08847.1. BC009406 mRNA. Translation: AAH09406.1.
IPI	IPI00006987.
RefSeq	NP_065147.1. NM_020414.3.
UniGene	Hs.510328.
3D structure databases
ProteinModelPortal	Q9GZR7.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47301N.
IntAct	Q9GZR7. 35 interactions.
MINT	MINT-1378437.
STRING	9606.ENSP00000328690.
PTM databases
PhosphoSite	Q9GZR7.
Polymorphism databases
DMDM	18202929.
2D gel databases
SWISS-2DPAGE	Q9GZR7.
Proteomic databases
PaxDb	Q9GZR7.
PeptideAtlas	Q9GZR7.
PRIDE	Q9GZR7.
Protocols and materials databases
DNASU	57062.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000330836; ENSP00000328690; ENSG00000089737.
GeneID	57062.
KEGG	hsa:57062.
UCSC	uc001ycj.3. human.
Organism-specific databases
CTD	57062.
GeneCards	GC14M094517.
HGNC	HGNC:13266. DDX24.
HPA	HPA002554.
MIM	606181. gene.
neXtProt	NX_Q9GZR7.
PharmGKB	PA27211.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0513.
HOGENOM	HOG000290702.
HOVERGEN	HBG104200.
InParanoid	Q9GZR7.
KO	K14805.
OMA	DQQEERR.
PhylomeDB	Q9GZR7.
Gene expression databases
ArrayExpress	Q9GZR7.
Bgee	Q9GZR7.
CleanEx	HS_DDX24.
Genevestigator	Q9GZR7.
GermOnline	ENSG00000089737. Homo sapiens.
Family and domain databases
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR027417. P-loop_NTPase. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 2 hits.
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	DDX24. human.
GenomeRNAi	57062.
NextBio	62861.
SOURCE	Search...

Entry information

Entry name

DDX24_HUMAN

Accession

Primary (citable) accession number: Q9GZR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	March 1, 2001
Last modified:	May 29, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.