Q9GZR2 (REXO4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA exonuclease 4
EC=3.1.-.-

Alternative name(s):
Exonuclease XPMC2
Prevents mitotic catastrophe 2 protein homolog
Short name=hPMC2

Gene names

Name:	REXO4
Synonyms:	PMC2, XPMC2H

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Can bind ESR1 and ESR2. This interaction is abrogated by estrogen and augmented by tamoxifen treatment.
Subcellular location	Nucleus › nucleolus Ref.2 Ref.8.
Sequence similarities	Belongs to the REXO4 family. Contains 1 exonuclease domain.
Sequence caution	The sequence CAI12845.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI12847.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI12848.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Exonuclease Hydrolase Nuclease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	nucleolus Inferred from direct assay. Source: LIFEdb
Molecular_function	exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Non-traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9GZR2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9GZR2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     76-132: WLLKQKSQAP...KDQEASRGSV → VPRWTGGRQY...RRQPQPHPPR
     133-304: Missing.

Note: Gene prediction based on EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	RNA exonuclease 4		PRO_0000131703
Regions
	Domain	243 – 394	152	Exonuclease
Amino acid modifications
	Modified residue	15	1	Phosphoserine Ref.9
Natural variations
	Alternative sequence	76 – 132	57	WLLKQ…SRGSV → VPRWTGGRQYLAPRPVEQST IRKEPRKGQMVILFQNEGTS SIRSGKLRRQPQPHPPR in isoform 2.		VSP_014796
	Alternative sequence	133 – 304	172	Missing in isoform 2.		VSP_014797
	Natural variant	141	1	R → K. Corresponds to variant rs6597630 [ dbSNP | Ensembl ].		VAR_023067
	Natural variant	283	1	T → A. Ref.1 Corresponds to variant rs2285487 [ dbSNP | Ensembl ].		VAR_023068
Experimental info
	Sequence conflict	202	1	A → P Ref.1
	Sequence conflict	202	1	A → P Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: 5994D59678B17C21	FASTA	422	46,672
	10 20 30 40 50 60 MGKAKVPASK RAPSSPVAKP GPVKTLTRKK NKKKKRFWKS KAREVSKKPA SGPGAVVRPP 70 80 90 100 110 120 KAPEDFSQNW KALQEWLLKQ KSQAPEKPLV ISQMGSKKKP KIIQQNKKET SPQVKGEEMP 130 140 150 160 170 180 AGKDQEASRG SVPSGSKMDR RAPVPRTKAS GTEHNKKGTK ERTNGDIVPE RGDIEHKKRK 190 200 210 220 230 240 AKEAAPAPPT EEDIWFDDVD PADIEAAIGP EAAKIARKQL GQSEGSVSLS LVKEQAFGGL 250 260 270 280 290 300 TRALALDCEM VGVGPKGEES MAARVSIVNQ YGKCVYDKYV KPTEPVTDYR TAVSGIRPEN 310 320 330 340 350 360 LKQGEELEVV QKEVAEMLKG RILVGHALHN DLKVLFLDHP KKKIRDTQKY KPFKSQVKSG 370 380 390 400 410 420 RPSLRLLSEK ILGLQVQQAE HCSIQDAQAA MRLYVMVKKE WESMARDRRP LLTAPDHCSD DA
	Isoform 2 [UniParc]. Checksum: F2F05F07242D6EFE	FASTA	250	28,390
	10 20 30 40 50 60 MGKAKVPASK RAPSSPVAKP GPVKTLTRKK NKKKKRFWKS KAREVSKKPA SGPGAVVRPP 70 80 90 100 110 120 KAPEDFSQNW KALQEVPRWT GGRQYLAPRP VEQSTIRKEP RKGQMVILFQ NEGTSSIRSG 130 140 150 160 170 180 KLRRQPQPHP PREELEVVQK EVAEMLKGRI LVGHALHNDL KVLFLDHPKK KIRDTQKYKP 190 200 210 220 230 240 FKSQVKSGRP SLRLLSEKIL GLQVQQAEHC SIQDAQAAMR LYVMVKKEWE SMARDRRPLL 250 TAPDHCSDDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human XPMC2H: cDNA cloning, mapping to 9q34, genomic structure, and evaluation as TSC1." Kwiatkowska J., Slomski R., Jozwiak S., Short M.P., Kwiatkowski D.J. Genomics 44:350-354(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-283.
[2]	"Identification and characterization of a novel factor that regulates quinone reductase gene transcriptional activity." Montano M.M., Wittmann B.M., Bianco N.R. J. Biol. Chem. 275:34306-34313(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, INTERACTION WITH ESR1 AND ESR2.
[3]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 2).
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[8]	"Functional proteomic analysis of human nucleolus." Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J. Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF295774 mRNA. Translation: AAG02123.1. AF273304 mRNA. Translation: AAF98162.1. AL136894 mRNA. Translation: CAB66828.1. AK025493 mRNA. Translation: BAB15152.1. AK314333 mRNA. Translation: BAG36979.1. AL158826 Genomic DNA. Translation: CAI12845.1. Sequence problems. AL158826 Genomic DNA. Translation: CAI12847.1. Sequence problems. AL158826 Genomic DNA. Translation: CAI12848.1. Sequence problems. AL158826 Genomic DNA. Translation: CAI12849.1. CH471090 Genomic DNA. Translation: EAW88082.1. BC009274 mRNA. Translation: AAH09274.1.
IPI	IPI00010653. IPI00513877.
RefSeq	NP_065118.2. NM_020385.2.
UniGene	Hs.438409.
3D structure databases
ProteinModelPortal	Q9GZR2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9GZR2. 1 interaction.
STRING	9606.ENSP00000361010.
PTM databases
PhosphoSite	Q9GZR2.
Polymorphism databases
DMDM	71153418.
2D gel databases
SWISS-2DPAGE	Q9GZR2.
Proteomic databases
PaxDb	Q9GZR2.
PRIDE	Q9GZR2.
Protocols and materials databases
DNASU	57109.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000371935; ENSP00000361003; ENSG00000148300. ENST00000371942; ENSP00000361010; ENSG00000148300. ENST00000445916; ENSP00000391534; ENSG00000148300. ENST00000453165; ENSP00000403272; ENSG00000148300. ENST00000454825; ENSP00000394229; ENSG00000148300. ENST00000562216; ENSP00000457846; ENSG00000261493. ENST00000568937; ENSP00000456472; ENSG00000261493. ENST00000569763; ENSP00000454220; ENSG00000261493. ENST00000570226; ENSP00000456823; ENSG00000261493.
GeneID	57109.
KEGG	hsa:57109.
UCSC	uc004cdm.3. human.
Organism-specific databases
CTD	57109.
GeneCards	GC09M136271.
HGNC	HGNC:12820. REXO4.
HPA	HPA047006.
MIM	602930. gene.
neXtProt	NX_Q9GZR2.
PharmGKB	PA37414.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0847.
HOGENOM	HOG000231626.
HOVERGEN	HBG000999.
InParanoid	Q9GZR2.
KO	K01175.
OMA	QKEVAEM.
OrthoDB	EOG4VX263.
PhylomeDB	Q9GZR2.
Gene expression databases
ArrayExpress	Q9GZR2.
Bgee	Q9GZR2.
CleanEx	HS_REXO4.
Genevestigator	Q9GZR2.
GermOnline	ENSG00000148300. Homo sapiens.
Family and domain databases
InterPro	IPR006055. Exonuclease. IPR013520. Exonuclease_RNaseT/DNA_pol3. IPR012337. RNaseH-like_dom. [Graphical view]
Pfam	PF00929. RNase_T. 1 hit. [Graphical view]
SMART	SM00479. EXOIII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	57109.
NextBio	62959.
SOURCE	Search...

Entry information

Entry name

REXO4_HUMAN

Accession

Primary (citable) accession number: Q9GZR2
Secondary accession number(s): B2RAT2 , Q5T8S4, Q5T8S5, Q5T8S6, Q9GZW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.