Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Microtubule-associated proteins 1A/1B light chain 3B

Gene

MAP1LC3B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.3 Publications

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • autophagosome assembly Source: GO_Central
  • autophagosome maturation Source: Reactome
  • autophagy Source: CAFA
  • cellular response to nitrogen starvation Source: GO_Central
  • macroautophagy Source: Reactome
  • membrane organization Source: Reactome
  • mitophagy Source: ParkinsonsUK-UCL

Keywordsi

Biological processAutophagy, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-1632852. Macroautophagy.
R-HSA-5205685. Pink/Parkin Mediated Mitophagy.
R-HSA-8854214. TBC/RABGAPs.
R-HSA-8934903. Receptor Mediated Mitophagy.
SIGNORiQ9GZQ8.

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated proteins 1A/1B light chain 3B
Alternative name(s):
Autophagy-related protein LC3 B
Autophagy-related ubiquitin-like modifier LC3 B
MAP1 light chain 3-like protein 2
MAP1A/MAP1B light chain 3 B
Short name:
MAP1A/MAP1B LC3 B
Microtubule-associated protein 1 light chain 3 beta
Gene namesi
Name:MAP1LC3B
Synonyms:MAP1ALC3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:13352. MAP1LC3B.

Subcellular locationi

GO - Cellular componenti

  • autophagosome Source: UniProtKB
  • autophagosome membrane Source: GO_Central
  • axoneme Source: UniProtKB
  • cytoplasmic vesicle Source: UniProtKB-KW
  • cytosol Source: GO_Central
  • endomembrane system Source: UniProtKB-SubCell
  • intracellular Source: LIFEdb
  • microtubule Source: UniProtKB-KW
  • mitochondrion Source: ParkinsonsUK-UCL
  • organelle membrane Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52 – 53FL → AA: No effect on interaction with TECPR2. 1 Publication2
Mutagenesisi68R → A: Impairs cleavage by ATG4B. 1 Publication1
Mutagenesisi70R → A: Abolishes interaction with TECPR2. 1 Publication1
Mutagenesisi120G → A: No processing of precursor. 1 Publication1
Mutagenesisi122K → A: No effect on processing of precursor. 1 Publication1

Organism-specific databases

DisGeNETi81631.
OpenTargetsiENSG00000140941.
PharmGKBiPA134923100.

Polymorphism and mutation databases

BioMutaiMAP1LC3B.
DMDMi17433141.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000171982 – 120Microtubule-associated proteins 1A/1B light chain 3BAdd BLAST119
PropeptideiPRO_0000017199121 – 125Removed in mature form5

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12Phosphothreonine; by PKABy similarity1
Lipidationi120Phosphatidylethanolamine amidated glycine1 Publication1

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094).1 Publication
The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
Phosphorylation at Thr-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE) (By similarity). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei120 – 121Cleavage; by ATG4BBy similarity2

Keywords - PTMi

Lipoprotein, Phosphoprotein

Proteomic databases

EPDiQ9GZQ8.
MaxQBiQ9GZQ8.
PaxDbiQ9GZQ8.
PeptideAtlasiQ9GZQ8.
PRIDEiQ9GZQ8.
TopDownProteomicsiQ9GZQ8.

PTM databases

iPTMnetiQ9GZQ8.
PhosphoSitePlusiQ9GZQ8.
SwissPalmiQ9GZQ8.

Miscellaneous databases

PMAP-CutDBiQ9GZQ8.

Expressioni

Tissue specificityi

Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.1 Publication

Gene expression databases

BgeeiENSG00000140941.
CleanExiHS_MAP1LC3B.
ExpressionAtlasiQ9GZQ8. baseline and differential.
GenevisibleiQ9GZQ8. HS.

Organism-specific databases

HPAiCAB037216.
HPA043000.
HPA052474.
HPA052484.
HPA053767.

Interactioni

Subunit structurei

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity). Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin (PubMed:15095872). Interacts with FYCO1 (via C-terminus) (PubMed:20100911, PubMed:24089205). Interacts with TP53INP1 and TP53INP2 (PubMed:22470510). Interacts with TBC1D25 (PubMed:21383079). Directly interacts with SQSTM1; this interaction leads to MAP1LC3B recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy (PubMed:17580304, PubMed:24089205, PubMed:18524774). Interacts with ATG4B, MAPK15 and BNIP3 (PubMed:22948227, PubMed:23209295). Interacts with MAPB1, KEAP1, PCM1, OFD1, CEP131, and TECPR2 (PubMed:20562859, PubMed:24089205). Interacts with TBC1D5 (PubMed:22354992). Found in a complex with UBQLN1 and UBQLN2 (PubMed:20529957). Interacts with UBQLN4 (via STI1 1 and 2 domains). Interacts with UBQLN1 in the presence of UBQLN4 (PubMed:23459205). Interacts with ATG13 (PubMed:24290141). Interacts with RETREG2, RETREG1 and RETREG3 (PubMed:26040720).By similarity15 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi123565. 91 interactors.
DIPiDIP-29760N.
IntActiQ9GZQ8. 365 interactors.
MINTiMINT-1369587.
STRINGi9606.ENSP00000268607.

Structurei

Secondary structure

1125
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 4Combined sources3
Helixi7 – 10Combined sources4
Helixi13 – 26Combined sources14
Beta strandi30 – 37Combined sources8
Beta strandi42 – 44Combined sources3
Beta strandi51 – 55Combined sources5
Helixi60 – 70Combined sources11
Beta strandi75 – 77Combined sources3
Beta strandi80 – 83Combined sources4
Turni84 – 86Combined sources3
Turni89 – 91Combined sources3
Beta strandi92 – 94Combined sources3
Helixi95 – 102Combined sources8
Beta strandi109 – 114Combined sources6
Helixi116 – 119Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V49NMR-A1-120[»]
2LUENMR-A5-119[»]
2N9XNMR-A1-120[»]
2ZJDX-ray1.56A/C1-125[»]
3VTUX-ray1.60A2-119[»]
3VTVX-ray1.70A2-119[»]
3VTWX-ray2.52A/B/C2-119[»]
3WAOX-ray2.60A/B/C/D2-119[»]
3X0WX-ray2.71A/B2-119[»]
4WAAX-ray2.35A/B2-119[»]
5D94X-ray1.53A1-125[»]
5DCNX-ray2.00A2-119[»]
5GMVX-ray2.25A/B1-125[»]
5MS2X-ray2.47B1-119[»]
5MS5X-ray1.53A/B10-119[»]
5MS6X-ray1.90A/B2-119[»]
5V4KX-ray2.10A/B2-119[»]
ProteinModelPortaliQ9GZQ8.
SMRiQ9GZQ8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9GZQ8.

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654. Eukaryota.
ENOG4111JAT. LUCA.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiQ9GZQ8.
KOiK10435.
OMAiIRDQHPT.
OrthoDBiEOG091G18ST.
PhylomeDBiQ9GZQ8.
TreeFamiTF312964.

Family and domain databases

CDDicd01611. GABARAP. 1 hit.
InterProiView protein in InterPro
IPR004241. Atg8-like.
IPR029071. Ubiquitin-rel_dom.
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiView protein in Pfam
PF02991. Atg8. 1 hit.
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9GZQ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT
60 70 80 90 100
KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE
110 120
SEKDEDGFLY MVYASQETFG MKLSV
Length:125
Mass (Da):14,688
Last modified:January 23, 2007 - v3
Checksum:iBB141DEC7653E83F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61S → G in AAH67797 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF303888 mRNA. Translation: AAG23182.1.
AF183417 mRNA. Translation: AAG09686.1.
AF087871 mRNA. Translation: AAM10499.1.
AK025556 mRNA. Translation: BAB15169.1.
BC018634 mRNA. Translation: AAH18634.1.
BC041874 mRNA. Translation: AAH41874.1.
BC067797 mRNA. Translation: AAH67797.1.
CCDSiCCDS10960.1.
RefSeqiNP_073729.1. NM_022818.4.
UniGeneiHs.356061.

Genome annotation databases

EnsembliENST00000268607; ENSP00000268607; ENSG00000140941.
GeneIDi81631.
KEGGihsa:81631.
UCSCiuc002fjx.4. human.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMLP3B_HUMAN
AccessioniPrimary (citable) accession number: Q9GZQ8
Secondary accession number(s): Q6NW02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 169 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

PubMed:12740394 has shown that the protein is cleaved at Lys-122 but PubMed:15355958 has shown that the cleavage site is at Gly-120 as in other mammalian orthologs.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families