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Q9GZQ8 (MLP3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Microtubule-associated proteins 1A/1B light chain 3B
Alternative name(s):
Autophagy-related protein LC3 B
Autophagy-related ubiquitin-like modifier LC3 B
MAP1 light chain 3-like protein 2
MAP1A/MAP1B light chain 3 B
Short name=MAP1A/MAP1B LC3 B
Microtubule-associated protein 1 light chain 3 beta
Gene names
Name:MAP1LC3B
Synonyms:MAP1ALC3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length125 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Ref.11 Ref.20 Ref.21

Subunit structure

3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins By similarity. Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Interacts with FYCO1 (via C-terminus). Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D25. Directly interacts with SQSTM1; this interaction leads to MAP1LC3B recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy. Interacts with ATG4B, MAPK15 and BNIP3. Interacts with MAPB1, KEAP1, PCM1, OFD1, AZI1, and TECPR2. Ref.9 Ref.10 Ref.12 Ref.13 Ref.14 Ref.15 Ref.17 Ref.20 Ref.21

Subcellular location

Cytoplasmcytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicleautophagosome membrane; Lipid-anchor. Note: LC3-II binds to the autophagic membranes. Localizes also to discrete punctae along the ciliary axoneme By similarity. Ref.3 Ref.10 Ref.16

Tissue specificity

Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver. Ref.3

Post-translational modification

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (Ref.6). Ref.3 Ref.6 Ref.7 Ref.19

The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.

Phosphorylation at Thr-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE) By similarity. Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.

Sequence similarities

Belongs to the ATG8 family.

Caution

Ref.3 has shown that the protein is cleaved at Lys-122 but Ref.7 has shown that the cleavage site is at Gly-120 as in other mammalian orthologs.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 120119Microtubule-associated proteins 1A/1B light chain 3B
PRO_0000017198
Propeptide121 – 1255Removed in mature form
PRO_0000017199

Sites

Site120 – 1212Cleavage; by ATG4B By similarity

Amino acid modifications

Modified residue121Phosphothreonine; by PKA By similarity
Lipidation1201Phosphatidylethanolamine amidated glycine Probable

Experimental info

Mutagenesis52 – 532FL → AA: No effect on interaction with TECPR2.
Mutagenesis681R → A: Impairs cleavage by ATG4B. Ref.19
Mutagenesis701R → A: Abolishes interaction with TECPR2. Ref.13
Mutagenesis1201G → A: No processing of precursor. Ref.7
Mutagenesis1221K → A: No effect on processing of precursor. Ref.7
Sequence conflict611S → G in AAH67797. Ref.5

Secondary structure

......................... 125
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9GZQ8 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: BB141DEC7653E83F

FASTA12514,688
        10         20         30         40         50         60 
MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM 

        70         80         90        100        110        120 
SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG 


MKLSV 

« Hide

References

« Hide 'large scale' references
[1]"Gene expression profiles in human non-small and small-cell lung cancers."
Difilippantonio S., Chen Y., Pietas A., Schluens K., Pacyna-Gengelbach M., Deutschmann N., Padilla-Nash H.M., Ried T., Petersen I.
Eur. J. Cancer 39:1936-1947(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung carcinoma.
[2]"The gene expression profiling of sporadic pheochromocytoma and novel full-length cDNAs cloning."
Yang Y.-S., Song H.-D., Peng Y.-D., Huang Q.-H., Li R.-Y., Zhu Z.-D., Hu R.-M., Han Z.-G., Chen J.-L.
Endocr. Relat. Cancer 10:621-627(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pheochromocytoma.
[3]"Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B."
He H., Dang Y., Dai F., Guo Z., Wu J., She X., Pei Y., Chen Y., Ling W., Wu C., Zhao S., Liu J.O., Yu L.
J. Biol. Chem. 278:29278-29287(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, CLEAVAGE, TISSUE SPECIFICITY.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Hepatoma.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain, Pancreas and Testis.
[6]"HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates."
Tanida I., Sou Y.-S., Ezaki J., Minematsu-Ikeguchi N., Ueno T., Kominami E.
J. Biol. Chem. 279:36268-36276(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: LIPIDATION, CLEAVAGE BY ATG4B.
[7]"Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes."
Tanida I., Ueno T., Kominami E.
J. Biol. Chem. 279:47704-47710(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE, MUTAGENESIS OF GLY-120 AND LYS-122.
[8]"LC3 conjugation system in mammalian autophagy."
Tanida I., Ueno T., Kominami E.
Int. J. Biochem. Cell Biol. 36:2503-2518(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[9]"Caldendrin but not calmodulin binds to light chain 3 of MAP1A/B: an association with the microtubule cytoskeleton highlighting exclusive binding partners for neuronal Ca(2+)-sensor proteins."
Seidenbecher C.I., Landwehr M., Smalla K.H., Kreutz M., Dieterich D.C., Zuschratter W., Reissner C., Hammarback J.A., Bockers T.M., Gundelfinger E.D., Kreutz M.R.
J. Mol. Biol. 336:957-970(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CABP1.
[10]"p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy."
Pankiv S., Clausen T.H., Lamark T., Brech A., Bruun J.A., Outzen H., Overvatn A., Bjorkoy G., Johansen T.
J. Biol. Chem. 282:24131-24145(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SQSTM1, SUBCELLULAR LOCATION.
[11]"LC3 and GATE-16/GABARAP subfamilies are both essential yet act differently in autophagosome biogenesis."
Weidberg H., Shvets E., Shpilka T., Shimron F., Shinder V., Elazar Z.
EMBO J. 29:1792-1802(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"FYCO1 is a Rab7 effector that binds to LC3 and PI3P to mediate microtubule plus end-directed vesicle transport."
Pankiv S., Alemu E.A., Brech A., Bruun J.A., Lamark T., Overvatn A., Bjorkoy G., Johansen T.
J. Cell Biol. 188:253-269(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FYCO1.
[13]"Network organization of the human autophagy system."
Behrends C., Sowa M.E., Gygi S.P., Harper J.W.
Nature 466:68-76(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TECPR2, MUTAGENESIS OF 52-PHE-LYS-53 AND ARG-70.
[14]"OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation."
Itoh T., Kanno E., Uemura T., Waguri S., Fukuda M.
J. Cell Biol. 192:839-853(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TBC1D25.
[15]"MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins."
Colecchia D., Strambi A., Sanzone S., Iavarone C., Rossi M., Dall'Armi C., Piccioni F., Verrotti di Pianella A., Chiariello M.
Autophagy 8:1724-1740(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAPK15, PHOSPHORYLATION.
[16]"Identification of autophagosome-associated proteins and regulators by quantitative proteomic analysis and genetic screens."
Dengjel J., Hoyer-Hansen M., Nielsen M.O., Eisenberg T., Harder L.M., Schandorff S., Farkas T., Kirkegaard T., Becker A.C., Schroeder S., Vanselow K., Lundberg E., Nielsen M.M., Kristensen A.R., Akimov V., Bunkenborg J., Madeo F., Jaattela M., Andersen J.S.
Mol. Cell. Proteomics 11:M111.014035.1-M111.014035.17(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[17]"DOR/Tp53inp2 and Tp53inp1 constitute a metazoan gene family encoding dual regulators of autophagy and transcription."
Sancho A., Duran J., Garcia-Espana A., Mauvezin C., Alemu E.A., Lamark T., Macias M.J., Desalle R., Royo M., Sala D., Chicote J.U., Palacin M., Johansen T., Zorzano A.
PLoS ONE 7:E34034-E34034(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TP53INP1 AND TP53INP2.
[18]"The Legionella effector RavZ inhibits host autophagy through irreversible Atg8 deconjugation."
Choy A., Dancourt J., Mugo B., O'Connor T.J., Isberg R.R., Melia T.J., Roy C.R.
Science 338:1072-1076(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: DECONJUGATION BY LEGIONELLA RAVZ.
[19]"Arginine68 is an essential residue for the C-terminal cleavage of human Atg8 family proteins."
Liu C., Ma H., Wu J., Huang Q., Liu J.O., Yu L.
BMC Cell Biol. 14:27-27(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ARG-68, CLEAVAGE BY ATG4B.
[20]"Modulation of serines 17 and 24 in the LC3-interacting region of Bnip3 determines pro-survival mitophagy versus apoptosis."
Zhu Y., Massen S., Terenzio M., Lang V., Chen-Lindner S., Eils R., Novak I., Dikic I., Hamacher-Brady A., Brady N.R.
J. Biol. Chem. 288:1099-1113(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH BNIP3, FUNCTION.
[21]"Autophagy promotes primary ciliogenesis by removing OFD1 from centriolar satellites."
Tang Z., Lin M.G., Stowe T.R., Chen S., Zhu M., Stearns T., Franco B., Zhong Q.
Nature 502:254-257(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAPB1; FYCO1; SQSTM1; KEAP1; PCM1; OFD1 AND AZI1, FUNCTION.
[22]"Solution structure of microtubule-associated protein light chain 3 and identification of its functional subdomains."
Kouno T., Mizuguchi M., Tanida I., Ueno T., Kanematsu T., Mori Y., Shinoda H., Hirata M., Kominami E., Kawano K.
J. Biol. Chem. 280:24610-24617(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 1-120.
[23]"Structural basis for sorting mechanism of p62 in selective autophagy."
Ichimura Y., Kumanomidou T., Sou Y.S., Mizushima T., Ezaki J., Ueno T., Kominami E., Yamane T., Tanaka K., Komatsu M.
J. Biol. Chem. 283:22847-22857(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.56 ANGSTROMS) IN COMPLEX WITH AN LC3 RECOGNITION SEQUENCE (LRS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF303888 mRNA. Translation: AAG23182.1.
AF183417 mRNA. Translation: AAG09686.1.
AF087871 mRNA. Translation: AAM10499.1.
AK025556 mRNA. Translation: BAB15169.1.
BC018634 mRNA. Translation: AAH18634.1.
BC041874 mRNA. Translation: AAH41874.1.
BC067797 mRNA. Translation: AAH67797.1.
RefSeqNP_073729.1. NM_022818.4.
XP_005256241.1. XM_005256184.1.
UniGeneHs.356061.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1V49NMR-A1-120[»]
2LUENMR-A5-119[»]
2ZJDX-ray1.56A/C1-125[»]
3VTUX-ray1.60A2-119[»]
3VTVX-ray1.70A2-119[»]
3VTWX-ray2.52A/B/C2-119[»]
3WAOX-ray2.60A/B/C/D2-119[»]
ProteinModelPortalQ9GZQ8.
SMRQ9GZQ8. Positions 1-122.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123565. 74 interactions.
DIPDIP-29760N.
IntActQ9GZQ8. 358 interactions.
MINTMINT-1369587.
STRING9606.ENSP00000268607.

PTM databases

PhosphoSiteQ9GZQ8.

Polymorphism databases

DMDM17433141.

Proteomic databases

PaxDbQ9GZQ8.
PRIDEQ9GZQ8.

Protocols and materials databases

DNASU81631.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000268607; ENSP00000268607; ENSG00000140941.
GeneID81631.
KEGGhsa:81631.
UCSCuc002fjx.3. human.

Organism-specific databases

CTD81631.
GeneCardsGC16P087425.
HGNCHGNC:13352. MAP1LC3B.
HPACAB037216.
MIM609604. gene.
neXtProtNX_Q9GZQ8.
PharmGKBPA134923100.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG283348.
HOGENOMHOG000232034.
HOVERGENHBG051706.
InParanoidQ9GZQ8.
KOK10435.
OMAIRDQHPT.
OrthoDBEOG7XH6RV.
PhylomeDBQ9GZQ8.
TreeFamTF312964.

Gene expression databases

ArrayExpressQ9GZQ8.
BgeeQ9GZQ8.
CleanExHS_MAP1LC3B.
GenevestigatorQ9GZQ8.

Family and domain databases

InterProIPR004241. Atg8_like.
[Graphical view]
PANTHERPTHR10969. PTHR10969. 1 hit.
PfamPF02991. Atg8. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMAP1LC3B. human.
EvolutionaryTraceQ9GZQ8.
GeneWikiMAP1LC3B.
GenomeRNAi81631.
NextBio72018.
PMAP-CutDBQ9GZQ8.
PROQ9GZQ8.
SOURCESearch...

Entry information

Entry nameMLP3B_HUMAN
AccessionPrimary (citable) accession number: Q9GZQ8
Secondary accession number(s): Q6NW02
Entry history
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM