Q9GZQ8 (MLP3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Microtubule-associated proteins 1A/1B light chain 3B

Alternative name(s):
Autophagy-related protein LC3 B
Autophagy-related ubiquitin-like modifier LC3 B
MAP1 light chain 3-like protein 2
MAP1A/MAP1B light chain 3 B
Short name=MAP1A/MAP1B LC3 B
Microtubule-associated protein 1 light chain 3 beta

Gene names

Name:	MAP1LC3B
Synonyms:	MAP1ALC3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in formation of autophagosomal vacuoles (autophagosomes).
Subunit structure	3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins By similarity. Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Interacts with FYCO1 (via C-terminus). Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D25. Directly interacts with SQSTM1; this interaction leads to MAP1LC3B recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy. Ref.9 Ref.10 Ref.11 Ref.12 Ref.13
Subcellular location	Cytoplasm › cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle › autophagosome membrane; Lipid-anchor. Note: LC3-II binds to the autophagic membranes. Ref.3 Ref.10
Tissue specificity	Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver. Ref.3
Post-translational modification	The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II. Ref.6 Ref.7
Sequence similarities	Belongs to the MAP1 LC3 family.
Caution	Ref.3 has shown that the protein is cleaved at Lys-122 but Ref.7 has shown that the cleavage site is at Gly-120 as in other mammalian orthologs.

Ontologies

Keywords
Biological process	Autophagy Ubl conjugation pathway
Cellular component	Cytoplasm Cytoplasmic vesicle Cytoskeleton Membrane Microtubule
PTM	Lipoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	autophagic vacuole Inferred from sequence or structural similarity Ref.3. Source: UniProtKB autophagic vacuole membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW organelle membrane Inferred from sequence or structural similarity Ref.3. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG13	O75143	2	EBI-373144,EBI-2798775
ATG16L1	Q676U5	2	EBI-373144,EBI-535909
ATG2A	Q2TAZ0	2	EBI-373144,EBI-2514077
ATG3	Q9NT62	7	EBI-373144,EBI-988094
ATG4B	Q9Y4P1	6	EBI-373144,EBI-712014
ATG5	Q9H1Y0	2	EBI-373144,EBI-1047414
ATG7	O95352	7	EBI-373144,EBI-987834
CLINT1	Q14677	2	EBI-373144,EBI-1171113
FYCO1	Q9BQS8	7	EBI-373144,EBI-2869338
GBAS	O75323	4	EBI-373144,EBI-307133
HADHA	P40939	4	EBI-373144,EBI-356720
IPO5	O00410	2	EBI-373144,EBI-356424
KBTBD6	Q86V97	2	EBI-373144,EBI-2514778
KBTBD7	Q8WVZ9	3	EBI-373144,EBI-473695
NBR1	Q14596	8	EBI-373144,EBI-742698
Nbr1	P97432	2	EBI-373144,EBI-642554	From a different organism.
NEK9	Q8TD19	2	EBI-373144,EBI-1044009
RASSF1	Q9NS23	2	EBI-373144,EBI-367363
RASSF5	Q8WWW0	5	EBI-373144,EBI-367390
SAFB2	Q14151	3	EBI-373144,EBI-352869
SQSTM1	Q13501	12	EBI-373144,EBI-307104
Sqstm1	Q64337	6	EBI-373144,EBI-645025	From a different organism.
STBD1	O95210	2	EBI-373144,EBI-2947137
STK3	Q13188	5	EBI-373144,EBI-992580
STK4	Q13043	7	EBI-373144,EBI-367376
TBC1D15	Q8TC07	2	EBI-373144,EBI-1048247
TBC1D2B	Q9UPU7	3	EBI-373144,EBI-2947180
UBA5	Q9GZZ9	2	EBI-373144,EBI-747805
ULK1	O75385	2	EBI-373144,EBI-908831

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 120

119

Microtubule-associated proteins 1A/1B light chain 3B

PRO_0000017198

Propeptide

121 – 125

5

Removed in mature form

PRO_0000017199

Amino acid modifications

Lipidation

120

1

Phosphatidylethanolamine amidated glycine Probable

Experimental info

Mutagenesis

120

1

G → A: No processing of precursor. Ref.7

Mutagenesis

122

1

K → A: No effect on processing of precursor. Ref.7

Sequence conflict

61

1

S → G in AAH67797. Ref.5

Secondary structure

1

.

.

.

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.

.

.

.

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.

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.

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.

.

.

.

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125

Helix Strand Turn

Details...

Helix

7 – 10

4

Helix

13 – 26

14

Beta strand

30 – 37

8

Beta strand

51 – 55

5

Helix

60 – 71

12

Turn

75 – 77

3

Beta strand

79 – 83

5

Turn

84 – 86

3

Helix

95 – 102

8

Beta strand

109 – 115

7

Helix

116 – 121

6

Sequences

	Sequence		Length	Mass (Da)
	Q9GZQ8 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: BB141DEC7653E83F	FASTA	125	14,688
	10 20 30 40 50 60 MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM 70 80 90 100 110 120 SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG MKLSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene expression profiles in human non-small and small-cell lung cancers." Difilippantonio S., Chen Y., Pietas A., Schluens K., Pacyna-Gengelbach M., Deutschmann N., Padilla-Nash H.M., Ried T., Petersen I. Eur. J. Cancer 39:1936-1947(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung carcinoma.
[2]	"The gene expression profiling of sporadic pheochromocytoma and novel full-length cDNAs cloning." Yang Y.-S., Song H.-D., Peng Y.-D., Huang Q.-H., Li R.-Y., Zhu Z.-D., Hu R.-M., Han Z.-G., Chen J.-L. Endocr. Relat. Cancer 10:621-627(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pheochromocytoma.
[3]	"Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." He H., Dang Y., Dai F., Guo Z., Wu J., She X., Pei Y., Chen Y., Ling W., Wu C., Zhao S., Liu J.O., Yu L. J. Biol. Chem. 278:29278-29287(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hepatoma.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Pancreas and Testis.
[6]	"HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates." Tanida I., Sou Y.-S., Ezaki J., Minematsu-Ikeguchi N., Ueno T., Kominami E. J. Biol. Chem. 279:36268-36276(2004) [PubMed] [Europe PMC] [Abstract] Cited for: LIPIDATION, CLEAVAGE BY APG4B.
[7]	"Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes." Tanida I., Ueno T., Kominami E. J. Biol. Chem. 279:47704-47710(2004) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE, MUTAGENESIS OF GLY-120 AND LYS-122.
[8]	"LC3 conjugation system in mammalian autophagy." Tanida I., Ueno T., Kominami E. Int. J. Biochem. Cell Biol. 36:2503-2518(2004) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[9]	"Caldendrin but not calmodulin binds to light chain 3 of MAP1A/B: an association with the microtubule cytoskeleton highlighting exclusive binding partners for neuronal Ca(2+)-sensor proteins." Seidenbecher C.I., Landwehr M., Smalla K.H., Kreutz M., Dieterich D.C., Zuschratter W., Reissner C., Hammarback J.A., Bockers T.M., Gundelfinger E.D., Kreutz M.R. J. Mol. Biol. 336:957-970(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CABP1.
[10]	"p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy." Pankiv S., Clausen T.H., Lamark T., Brech A., Bruun J.A., Outzen H., Overvatn A., Bjorkoy G., Johansen T. J. Biol. Chem. 282:24131-24145(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SQSTM1, SUBCELLULAR LOCATION.
[11]	"FYCO1 is a Rab7 effector that binds to LC3 and PI3P to mediate microtubule plus end-directed vesicle transport." Pankiv S., Alemu E.A., Brech A., Bruun J.A., Lamark T., Overvatn A., Bjorkoy G., Johansen T. J. Cell Biol. 188:253-269(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FYCO1.
[12]	"OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation." Itoh T., Kanno E., Uemura T., Waguri S., Fukuda M. J. Cell Biol. 192:839-853(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TBC1D25.
[13]	"DOR/Tp53inp2 and Tp53inp1 constitute a metazoan gene family encoding dual regulators of autophagy and transcription." Sancho A., Duran J., Garcia-Espana A., Mauvezin C., Alemu E.A., Lamark T., Macias M.J., Desalle R., Royo M., Sala D., Chicote J.U., Palacin M., Johansen T., Zorzano A. PLoS ONE 7:E34034-E34034(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TP53INP1 AND TP53INP2.
[14]	"Solution structure of microtubule-associated protein light chain 3 and identification of its functional subdomains." Kouno T., Mizuguchi M., Tanida I., Ueno T., Kanematsu T., Mori Y., Shinoda H., Hirata M., Kominami E., Kawano K. J. Biol. Chem. 280:24610-24617(2005) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 1-120.
[15]	"Structural basis for sorting mechanism of p62 in selective autophagy." Ichimura Y., Kumanomidou T., Sou Y.S., Mizushima T., Ezaki J., Ueno T., Kominami E., Yamane T., Tanaka K., Komatsu M. J. Biol. Chem. 283:22847-22857(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.56 ANGSTROMS) IN COMPLEX WITH AN LC3 RECOGNITION SEQUENCE (LRS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF303888 mRNA. Translation: AAG23182.1.
AF183417 mRNA. Translation: AAG09686.1.
AF087871 mRNA. Translation: AAM10499.1.
AK025556 mRNA. Translation: BAB15169.1.
BC018634 mRNA. Translation: AAH18634.1.
BC041874 mRNA. Translation: AAH41874.1.
BC067797 mRNA. Translation: AAH67797.1.

IPI

IPI00220482.

RefSeq

NP_073729.1. NM_022818.4.

UniGene

Hs.356061.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1V49	NMR	-	A	1-120	[»]
2ZJD	X-ray	1.56	A/C	1-125	[»]

ProteinModelPortal

Q9GZQ8.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29760N.

IntAct

Q9GZQ8. 323 interactions.

MINT

MINT-1369587.

STRING

9606.ENSP00000268607.

PTM databases

PhosphoSite

Q9GZQ8.

Polymorphism databases

DMDM

17433141.

Proteomic databases

PaxDb

Q9GZQ8.

PRIDE

Q9GZQ8.

Protocols and materials databases

DNASU

81631.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000268607; ENSP00000268607; ENSG00000140941.

GeneID

81631.

KEGG

hsa:81631.

UCSC

uc002fjx.3. human.

Organism-specific databases

CTD

81631.

GeneCards

GC16P087425.

HGNC

HGNC:13352. MAP1LC3B.

HPA

CAB037216.

MIM

609604. gene.

neXtProt

NX_Q9GZQ8.

PharmGKB

PA134923100.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG283348.

HOGENOM

HOG000232034.

HOVERGEN

HBG051706.

InParanoid

Q9GZQ8.

KO

K10435.

OMA

IRSQQPN.

OrthoDB

EOG4PC9TJ.

PhylomeDB

Q9GZQ8.

Gene expression databases

ArrayExpress

Q9GZQ8.

Bgee

Q9GZQ8.

CleanEx

HS_MAP1LC3B.

Genevestigator

Q9GZQ8.

GermOnline

ENSG00000140941. Homo sapiens.

Family and domain databases

InterPro

IPR004241. Atg8_ubiquitin-like.
[Graphical view]

PANTHER

PTHR10969. PTHR10969. 1 hit.

Pfam

PF02991. Atg8. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

MAP1LC3B. human.

EvolutionaryTrace

Q9GZQ8.

GenomeRNAi

81631.

NextBio

72018.

PMAP-CutDB

Q9GZQ8.

SOURCE

Search...

Entry information

Entry name

MLP3B_HUMAN

Accession

Primary (citable) accession number: Q9GZQ8
Secondary accession number(s): Q6NW02

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 5, 2001
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 127 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.