Q9GZQ4 (NMUR2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuromedin-U receptor 2
Short name=NMU-R2

Alternative name(s):
G-protein coupled receptor FM-4
G-protein coupled receptor TGR-1

Gene names

Name:	NMUR2
Synonyms:	NMU2R, TGR1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for the neuromedin-U and neuromedin-S neuropeptides By similarity. Ref.2
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Predominantly expressed in the CNS, particularly in the medulla oblongata, pontine reticular formation, spinal cord, and thalamus. High level in testis whereas lower levels are present in a variety of peripheral tissues including the gastrointestinal tract, genitourinary tract, liver, pancreas, adrenal gland, thyroid gland, lung, trachea, spleen and thymus. Ref.1 Ref.2 Ref.3 Ref.6
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Caution	It is uncertain whether Met-1 or Met-4 is the initiator.
Sequence caution	The sequence BAB13721.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of phospholipase A2 activity by calcium-mediated signaling Inferred from direct assay Ref.2. Source: UniProtKB arachidonic acid secretion Inferred from direct assay Ref.1. Source: UniProtKB calcium ion transport Inferred from direct assay Ref.2. Source: UniProtKB central nervous system development Inferred from expression pattern Ref.2. Source: UniProtKB elevation of cytosolic calcium ion concentration Inferred from direct assay Ref.2. Source: UniProtKB feeding behavior Traceable author statement Ref.6. Source: ProtInc grooming behavior Inferred from electronic annotation. Source: Compara inositol phosphate-mediated signaling Inferred from direct assay Ref.2. Source: UniProtKB phospholipase C-activating G-protein coupled receptor signaling pathway Inferred from direct assay Ref.2. Source: UniProtKB reduction of food intake in response to dietary excess Inferred from electronic annotation. Source: Compara regulation of smooth muscle contraction Non-traceable author statement Ref.1. Source: UniProtKB response to pain Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from direct assay Ref.2. Source: UniProtKB plasma membrane Traceable author statement. Source: Reactome
Molecular_function	GTP binding Inferred from direct assay Ref.2. Source: UniProtKB intracellular calcium activated chloride channel activity Inferred from direct assay Ref.2. Source: UniProtKB neuromedin U binding Inferred from direct assay Ref.2. Source: UniProtKB neuromedin U receptor activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Neuromedin-U receptor 2		PRO_0000069910
Regions
	Topological domain	1 – 49	49	Extracellular Potential
	Transmembrane	50 – 70	21	Helical; Name=1; Potential
	Topological domain	71 – 82	12	Cytoplasmic Potential
	Transmembrane	83 – 103	21	Helical; Name=2; Potential
	Topological domain	104 – 123	20	Extracellular Potential
	Transmembrane	124 – 146	23	Helical; Name=3; Potential
	Topological domain	147 – 165	19	Cytoplasmic Potential
	Transmembrane	166 – 186	21	Helical; Name=4; Potential
	Topological domain	187 – 214	28	Extracellular Potential
	Transmembrane	215 – 235	21	Helical; Name=5; Potential
	Topological domain	236 – 265	30	Cytoplasmic Potential
	Transmembrane	266 – 286	21	Helical; Name=6; Potential
	Topological domain	287 – 301	15	Extracellular Potential
	Transmembrane	302 – 322	21	Helical; Name=7; Potential
	Topological domain	323 – 415	93	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	9	1	N-linked (GlcNAc...) Potential
	Glycosylation	27	1	N-linked (GlcNAc...) Potential
	Glycosylation	194	1	N-linked (GlcNAc...) Potential
	Disulfide bond	119 ↔ 204		By similarity
Natural variations
	Natural variant	298	1	S → T. Corresponds to variant rs4958535 [ dbSNP | Ensembl ].		VAR_023941
	Natural variant	315	1	F → L. Corresponds to variant rs1895245 [ dbSNP | Ensembl ].		VAR_023942
	Natural variant	383	1	P → L. Corresponds to variant rs4958532 [ dbSNP | Ensembl ].		VAR_032770
	Natural variant	388	1	M → V. Corresponds to variant rs4958531 [ dbSNP | Ensembl ].		VAR_023943
	Natural variant	395	1	A → T. Ref.1 Ref.2 Ref.3 Ref.5 Ref.6 Corresponds to variant rs1363422 [ dbSNP | Ensembl ].		VAR_023944
Experimental info
	Sequence conflict	204	1	C → F in AAH16938. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9GZQ4 [UniParc]. Last modified November 2, 2010. Version 2. Checksum: C2BACD84A812890F	FASTA	415	47,696
	10 20 30 40 50 60 MSGMEKLQNA SWIYQQKLED PFQKHLNSTE EYLAFLCGPR RSHFFLPVSV VYVPIFVVGV 70 80 90 100 110 120 IGNVLVCLVI LQHQAMKTPT NYYLFSLAVS DLLVLLLGMP LEVYEMWRNY PFLFGPVGCY 130 140 150 160 170 180 FKTALFETVC FASILSITTV SVERYVAILH PFRAKLQSTR RRALRILGIV WGFSVLFSLP 190 200 210 220 230 240 NTSIHGIKFH YFPNGSLVPG SATCTVIKPM WIYNFIIQVT SFLFYLLPMT VISVLYYLMA 250 260 270 280 290 300 LRLKKDKSLE ADEGNANIQR PCRKSVNKML FVLVLVFAIC WAPFHIDRLF FSFVEEWSES 310 320 330 340 350 360 LAAVFNLVHV VSGVFFYLSS AVNPIIYNLL SRRFQAAFQN VISSFHKQWH SQHDPQLPPA 370 380 390 400 410 QRNIFLTECH FVELTEDIGP QFPCQSSMHN SHLPAALSSE QMSRTNYQSF HFNKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and functional characterization of a novel subtype of neuromedin U receptor." Hosoya M., Moriya T., Kawamata Y., Ohkubo S., Fujii R., Matsui H., Shintani Y., Fukusumi S., Habata Y., Hinuma S., Onda H., Nishimura O., Fujino M. J. Biol. Chem. 275:29528-29532(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT THR-395.
[2]	"Identification and characterization of two neuromedin U receptors differentially expressed in peripheral tissues and the central nervous system." Raddatz R., Wilson A.E., Artymyshyn R., Bonini J.A., Borowsky B., Boteju L.W., Zhou S., Kouranova E.V., Nagorny R., Guevarra M.S., Dai M., Lerman G.S., Vaysse P.J., Branchek T.A., Gerald C., Forray C., Adham N. J. Biol. Chem. 275:32452-32459(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, VARIANT THR-395.
[3]	"Identification of a novel neuromedin U receptor subtype expressed in the central nervous system." Shan L., Qiao X., Crona J.H., Behan J., Wang S., Laz T., Bayne M., Gustafson E.L., Monsma F.J. Jr., Hedrick J.A. J. Biol. Chem. 275:39482-39486(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT THR-395.
[4]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-395. Tissue: Colon and Placenta.
[6]	"Identification of receptors for neuromedin U and its role in feeding." Howard A.D., Wang R., Pong S.-S., Mellin T.N., Strack A., Guan X.-M., Zeng Z., Williams D.L., Feighner S.D., Nunes C.N., Murphy B., Stair J.N., Yu H., Jiang Q., Clements M.K., Tan C.P., Mckee K.K., Hreniuk D.L. , Mcdonald T.P., Lynch K.R., Evans J.F., Austin C.P., Caskey T., van der Ploeg L.H.T., Liu Q. Nature 406:70-74(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-415, TISSUE SPECIFICITY, VARIANT THR-395.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB041228 mRNA. Translation: BAB13721.1. Different initiation. AF272363 mRNA. Translation: AAG24794.1. AF292402 mRNA. Translation: AAG03064.1. AC008571 Genomic DNA. No translation available. BC016938 mRNA. Translation: AAH16938.1. BC067776 mRNA. Translation: AAH67776.1. AF242874 mRNA. Translation: AAF82755.1.
IPI	IPI00100753.
RefSeq	NP_064552.3. NM_020167.4.
UniGene	Hs.731911.
3D structure databases
ProteinModelPortal	Q9GZQ4.
SMR	Q9GZQ4. Positions 48-343.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000255262.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9GZQ4.
Polymorphism databases
DMDM	311033403.
Proteomic databases
PaxDb	Q9GZQ4.
PRIDE	Q9GZQ4.
Protocols and materials databases
DNASU	56923.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000255262; ENSP00000255262; ENSG00000132911.
GeneID	56923.
KEGG	hsa:56923.
UCSC	uc003luv.2. human.
Organism-specific databases
CTD	56923.
GeneCards	GC05M151751.
H-InvDB	HIX0005335.
HGNC	HGNC:16454. NMUR2.
HPA	HPA045836.
MIM	605108. gene.
neXtProt	NX_Q9GZQ4.
PharmGKB	PA31664.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG308321.
HOGENOM	HOG000113804.
HOVERGEN	HBG103506.
InParanoid	Q9GZQ4.
KO	K05053.
OMA	LFYILPM.
OrthoDB	EOG42822B.
PhylomeDB	Q9GZQ4.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	Q9GZQ4.
CleanEx	HS_NMUR2.
Genevestigator	Q9GZQ4.
GermOnline	ENSG00000132911. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR005390. NeuromedU_rcpt. IPR005392. NeuromedU_rcpt_2. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01565. NEUROMEDINUR. PR01567. NEUROMEDNU2R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1075144.
GenomeRNAi	56923.
NextBio	62432.
SOURCE	Search...

Entry information

Entry name

NMUR2_HUMAN

Accession

Primary (citable) accession number: Q9GZQ4
Secondary accession number(s): Q7LC54, Q96AM5, Q9NRA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	November 2, 2010
Last modified:	May 1, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.