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Protein

Brain-specific serine protease 4

Gene

PRSS22

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Preferentially cleaves the synthetic substrate H-D-Leu-Thr-Arg-pNA compared to tosyl-Gly-Pro-Arg-pNA.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901Charge relay systemBy similarity
Active sitei141 – 1411Charge relay systemBy similarity
Active sitei242 – 2421Charge relay systemBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.252.

Names & Taxonomyi

Protein namesi
Recommended name:
Brain-specific serine protease 4 (EC:3.4.21.-)
Short name:
BSSP-4
Alternative name(s):
Serine protease 22
Serine protease 26
Tryptase epsilon
Gene namesi
Name:PRSS22
Synonyms:BSSP4, PRSS26
ORF Names:SP001LA, UNQ302/PRO343
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:14368. PRSS22.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33835.

Polymorphism and mutation databases

BioMutaiPRSS22.
DMDMi18202927.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence analysisAdd
BLAST
Chaini33 – 317285Brain-specific serine protease 4PRO_0000027504Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi70 – 701N-linked (GlcNAc...)1 Publication
Disulfide bondi75 ↔ 91PROSITE-ProRule annotation
Disulfide bondi175 ↔ 248PROSITE-ProRule annotation
Disulfide bondi208 ↔ 227PROSITE-ProRule annotation
Disulfide bondi238 ↔ 266PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9GZN4.
PaxDbiQ9GZN4.
PeptideAtlasiQ9GZN4.
PRIDEiQ9GZN4.
TopDownProteomicsiQ9GZN4.

Expressioni

Tissue specificityi

Expressed abundantly in the epithelial cells of the airways, including trachea, esophagus and fetal lung. Scarce in adult lung. Expressed at low levels in placenta, pancreas, prostate and thyroid gland.1 Publication

Gene expression databases

BgeeiQ9GZN4.
CleanExiHS_PRSS22.
ExpressionAtlasiQ9GZN4. baseline and differential.
GenevisibleiQ9GZN4. HS.

Organism-specific databases

HPAiHPA049161.

Interactioni

Protein-protein interaction databases

BioGridi122036. 2 interactions.
STRINGi9606.ENSP00000161006.

Structurei

3D structure databases

ProteinModelPortaliQ9GZN4.
SMRiQ9GZN4. Positions 50-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 290241Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118810.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ9GZN4.
KOiK09626.
OMAiAHCFKGH.
OrthoDBiEOG75B84T.
PhylomeDBiQ9GZN4.
TreeFamiTF351676.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9GZN4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVSGAPPAL GGGCLGTFTS LLLLASTAIL NAARIPVPPA CGKPQQLNRV
60 70 80 90 100
VGGEDSTDSE WPWIVSIQKN GTHHCAGSLL TSRWVITAAH CFKDNLNKPY
110 120 130 140 150
LFSVLLGAWQ LGNPGSRSQK VGVAWVEPHP VYSWKEGACA DIALVRLERS
160 170 180 190 200
IQFSERVLPI CLPDASIHLP PNTHCWISGW GSIQDGVPLP HPQTLQKLKV
210 220 230 240 250
PIIDSEVCSH LYWRGAGQGP ITEDMLCAGY LEGERDACLG DSGGPLMCQV
260 270 280 290 300
DGAWLLAGII SWGEGCAERN RPGVYISLSA HRSWVEKIVQ GVQLRGRAQG
310
GGALRAPSQG SGAAARS
Length:317
Mass (Da):33,732
Last modified:March 1, 2001 - v1
Checksum:iE2A123BC86E79935
GO

Sequence cautioni

The sequence AAB93671.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAQ88762.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010779 mRNA. Translation: BAB20263.1.
AF321182 mRNA. Translation: AAG35070.1.
AY358396 mRNA. Translation: AAQ88762.1. Different initiation.
AC003965 Genomic DNA. Translation: AAB93671.1. Sequence problems.
BC009726 mRNA. Translation: AAH09726.1.
CCDSiCCDS10481.1.
RefSeqiNP_071402.1. NM_022119.3.
XP_005255530.1. XM_005255473.2.
UniGeneiHs.459709.

Genome annotation databases

EnsembliENST00000161006; ENSP00000161006; ENSG00000005001.
ENST00000634511; ENSP00000489576; ENSG00000282937.
GeneIDi64063.
KEGGihsa:64063.
UCSCiuc002cry.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010779 mRNA. Translation: BAB20263.1.
AF321182 mRNA. Translation: AAG35070.1.
AY358396 mRNA. Translation: AAQ88762.1. Different initiation.
AC003965 Genomic DNA. Translation: AAB93671.1. Sequence problems.
BC009726 mRNA. Translation: AAH09726.1.
CCDSiCCDS10481.1.
RefSeqiNP_071402.1. NM_022119.3.
XP_005255530.1. XM_005255473.2.
UniGeneiHs.459709.

3D structure databases

ProteinModelPortaliQ9GZN4.
SMRiQ9GZN4. Positions 50-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122036. 2 interactions.
STRINGi9606.ENSP00000161006.

Protein family/group databases

MEROPSiS01.252.

Polymorphism and mutation databases

BioMutaiPRSS22.
DMDMi18202927.

Proteomic databases

MaxQBiQ9GZN4.
PaxDbiQ9GZN4.
PeptideAtlasiQ9GZN4.
PRIDEiQ9GZN4.
TopDownProteomicsiQ9GZN4.

Protocols and materials databases

DNASUi64063.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000161006; ENSP00000161006; ENSG00000005001.
ENST00000634511; ENSP00000489576; ENSG00000282937.
GeneIDi64063.
KEGGihsa:64063.
UCSCiuc002cry.1. human.

Organism-specific databases

CTDi64063.
GeneCardsiPRSS22.
HGNCiHGNC:14368. PRSS22.
HPAiHPA049161.
MIMi609343. gene.
neXtProtiNX_Q9GZN4.
PharmGKBiPA33835.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118810.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ9GZN4.
KOiK09626.
OMAiAHCFKGH.
OrthoDBiEOG75B84T.
PhylomeDBiQ9GZN4.
TreeFamiTF351676.

Miscellaneous databases

GeneWikiiPRSS22.
GenomeRNAii64063.
PROiQ9GZN4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9GZN4.
CleanExiHS_PRSS22.
ExpressionAtlasiQ9GZN4. baseline and differential.
GenevisibleiQ9GZN4. HS.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a human brain-specific serine protease, hBSSP-4."
    Mitsui S., Okui A., Kominami K., Yamaguchi N.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Human tryptase epsilon (PRSS22), a new member of the chromosome 16p13.3 family of human serine proteases expressed in airway epithelial cells."
    Wong G.W., Yasuda S., Madhusudhan M.S., Li L., Yang Y., Krilis S.A., Sali A., Stevens R.L.
    J. Biol. Chem. 276:49169-49182(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION AT ASN-70.
    Tissue: Pancreas.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreas.

Entry informationi

Entry nameiBSSP4_HUMAN
AccessioniPrimary (citable) accession number: Q9GZN4
Secondary accession number(s): O43342, Q6UXE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: March 1, 2001
Last modified: July 6, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.