Q9GZN4 (BSSP4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Brain-specific serine protease 4
Short name=BSSP-4
EC=3.4.21.-

Alternative name(s):
Serine protease 22
Serine protease 26
Tryptase epsilon

Gene names

Name:	PRSS22
Synonyms:	BSSP4, PRSS26
ORF Names:	SP001LA, UNQ302/PRO343

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Preferentially cleaves the synthetic substrate H-D-Leu-Thr-Arg-pNA compared to tosyl-Gly-Pro-Arg-pNA. Ref.2
Subcellular location	Secreted Ref.2.
Tissue specificity	Expressed abundantly in the epithelial cells of the airways, including trachea, esophagus and fetal lung. Scarce in adult lung. Expressed at low levels in placenta, pancreas, prostate and thyroid gland. Ref.2
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.
Sequence caution	The sequence AAB93671.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAQ88762.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 317	285	Brain-specific serine protease 4		PRO_0000027504
Regions
	Domain	50 – 290	241	Peptidase S1
Sites
	Active site	90	1	Charge relay system By similarity
	Active site	141	1	Charge relay system By similarity
	Active site	242	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	70	1	N-linked (GlcNAc...) Ref.2
	Disulfide bond	75 ↔ 91		By similarity
	Disulfide bond	175 ↔ 248		By similarity
	Disulfide bond	208 ↔ 227		By similarity
	Disulfide bond	238 ↔ 266		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9GZN4 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: E2A123BC86E79935	FASTA	317	33,732
	10 20 30 40 50 60 MVVSGAPPAL GGGCLGTFTS LLLLASTAIL NAARIPVPPA CGKPQQLNRV VGGEDSTDSE 70 80 90 100 110 120 WPWIVSIQKN GTHHCAGSLL TSRWVITAAH CFKDNLNKPY LFSVLLGAWQ LGNPGSRSQK 130 140 150 160 170 180 VGVAWVEPHP VYSWKEGACA DIALVRLERS IQFSERVLPI CLPDASIHLP PNTHCWISGW 190 200 210 220 230 240 GSIQDGVPLP HPQTLQKLKV PIIDSEVCSH LYWRGAGQGP ITEDMLCAGY LEGERDACLG 250 260 270 280 290 300 DSGGPLMCQV DGAWLLAGII SWGEGCAERN RPGVYISLSA HRSWVEKIVQ GVQLRGRAQG 310 GGALRAPSQG SGAAARS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a human brain-specific serine protease, hBSSP-4." Mitsui S., Okui A., Kominami K., Yamaguchi N. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Human tryptase epsilon (PRSS22), a new member of the chromosome 16p13.3 family of human serine proteases expressed in airway epithelial cells." Wong G.W., Yasuda S., Madhusudhan M.S., Li L., Yang Y., Krilis S.A., Sali A., Stevens R.L. J. Biol. Chem. 276:49169-49182(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION AT ASN-70. Tissue: Pancreas.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB010779 mRNA. Translation: BAB20263.1. AF321182 mRNA. Translation: AAG35070.1. AY358396 mRNA. Translation: AAQ88762.1. Different initiation. AC003965 Genomic DNA. Translation: AAB93671.1. Sequence problems. BC009726 mRNA. Translation: AAH09726.1.
IPI	IPI00005467.
RefSeq	NP_071402.1. NM_022119.3.
UniGene	Hs.459709.
3D structure databases
ProteinModelPortal	Q9GZN4.
ModBase	Search...
Protein family/group databases
MEROPS	S01.252.
Polymorphism databases
DMDM	18202927.
Proteomic databases
PaxDb	Q9GZN4.
PRIDE	Q9GZN4.
Protocols and materials databases
DNASU	64063.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000161006; ENSP00000161006; ENSG00000005001.
GeneID	64063.
KEGG	hsa:64063.
UCSC	uc002cry.1. human.
Organism-specific databases
CTD	64063.
GeneCards	GC16M002902.
HGNC	HGNC:14368. PRSS22.
HPA	HPA049161.
MIM	609343. gene.
neXtProt	NX_Q9GZN4.
PharmGKB	PA33835.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	Q9GZN4.
KO	K09626.
OMA	TNCWIAG.
OrthoDB	EOG495ZSC.
PhylomeDB	Q9GZN4.
Gene expression databases
ArrayExpress	Q9GZN4.
Bgee	Q9GZN4.
CleanEx	HS_PRSS22.
Genevestigator	Q9GZN4.
GermOnline	ENSG00000005001. Homo sapiens.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	64063.
NextBio	65826.
SOURCE	Search...

Entry information

Entry name

BSSP4_HUMAN

Accession

Primary (citable) accession number: Q9GZN4
Secondary accession number(s): O43342, Q6UXE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.