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Protein

Homeobox protein TGIF2

Gene

TGIF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor, which probably repress transcription by binding directly the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins. Probably represses transcription via the recruitment of histone deacetylase proteins.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi16 – 7964Homeobox; TALE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-2173795. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
R-HSA-2173796. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein TGIF2
Alternative name(s):
5'-TG-3'-interacting factor 2
TGF-beta-induced transcription factor 2
Short name:
TGFB-induced factor 2
Gene namesi
Name:TGIF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:15764. TGIF2.

Subcellular locationi

  • Nucleus

  • Note: Excluded from nucleoli.

GO - Cellular componenti

  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi182 – 1821T → V: Decrease of phosphorylation. Strong decrease of phosphorylation; when associated with V-186. 1 Publication
Mutagenesisi186 – 1861T → V: Decrease of phosphorylation. Strong decrease of phosphorylation; when associated with V-182. 1 Publication

Organism-specific databases

PharmGKBiPA38035.

Polymorphism and mutation databases

BioMutaiTGIF2.
DMDMi27805750.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237Homeobox protein TGIF2PRO_0000049321Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei182 – 1821Phosphothreonine1 Publication
Modified residuei186 – 1861Phosphothreonine1 Publication

Post-translational modificationi

The C-terminal part is phosphorylated in response to EGF signaling by the Ras/MAPK pathway.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9GZN2.
MaxQBiQ9GZN2.
PaxDbiQ9GZN2.
PeptideAtlasiQ9GZN2.
PRIDEiQ9GZN2.

PTM databases

iPTMnetiQ9GZN2.
PhosphoSiteiQ9GZN2.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in heart, kidney and testis. Weakly expressed in brain and prostate.

Gene expression databases

BgeeiENSG00000118707.
CleanExiHS_TGIF2.
ExpressionAtlasiQ9GZN2. baseline and differential.
GenevisibleiQ9GZN2. HS.

Organism-specific databases

HPAiHPA053786.

Interactioni

Subunit structurei

Interacts with the transcriptional modulator SMAD3 and the histone deacetylase HDAC1.1 Publication

Protein-protein interaction databases

BioGridi121908. 5 interactions.
1529332. 1 interaction.
STRINGi9606.ENSP00000362979.

Structurei

3D structure databases

ProteinModelPortaliQ9GZN2.
SMRiQ9GZN2. Positions 25-102.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni103 – 237135Repressive functionAdd
BLAST

Sequence similaritiesi

Belongs to the TALE/TGIF homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0773. Eukaryota.
ENOG410XPMQ. LUCA.
GeneTreeiENSGT00390000004786.
ENSGT00550000074260.
HOGENOMiHOG000232039.
HOVERGENiHBG001143.
InParanoidiQ9GZN2.
KOiK19553.
OMAiFPQGELE.
OrthoDBiEOG091G0N1F.
PhylomeDBiQ9GZN2.
TreeFamiTF314558.
TF318093.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF05920. Homeobox_KN. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9GZN2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDSDLGEDE GLLSLAGKRK RRGNLPKESV KILRDWLYLH RYNAYPSEQE
60 70 80 90 100
KLSLSGQTNL SVLQICNWFI NARRRLLPDM LRKDGKDPNQ FTISRRGGKA
110 120 130 140 150
SDVALPRGSS PSVLAVSVPA PTNVLSLSVC SMPLHSGQGE KPAAPFPRGE
160 170 180 190 200
LESPKPLVTP GSTLTLLTRA EAGSPTGGLF NTPPPTPPEQ DKEDFSSFQL
210 220 230
LVEVALQRAA EMELQKQQDP SLPLLHTPIP LVSENPQ
Length:237
Mass (Da):25,878
Last modified:March 1, 2001 - v1
Checksum:iE78101CF7EF12555
GO
Isoform 2 (identifier: Q9GZN2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     65-155: ICNWFINARR...FPRGELESPK → DEFLDVIYWF...IIFYTAIHYD
     156-237: Missing.

Note: No experimental confirmation available.
Show »
Length:155
Mass (Da):17,908
Checksum:i8F3B33FF9F059D71
GO

Sequence cautioni

The sequence BX377269 differs from that shown. Reason: Frameshift at positions 87 and 118. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei65 – 15591ICNWF…LESPK → DEFLDVIYWFRQIIAVVLGV IWGVLPLRGFLGIAGFCLIN AGVLYLYFSNYLQIDEEEYG GTWELTKEGFMTSFALFMVI WIIFYTAIHYD in isoform 2. 1 PublicationVSP_047346Add
BLAST
Alternative sequencei156 – 23782Missing in isoform 2. 1 PublicationVSP_047347Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042646 mRNA. Translation: BAB16424.1.
BX377269 mRNA. No translation available.
AK074802 mRNA. Translation: BAC11216.1.
AK313919 mRNA. Translation: BAG36640.1.
AL050318 Genomic DNA. Translation: CAB75368.1.
CH471077 Genomic DNA. Translation: EAW76127.1.
CH471077 Genomic DNA. Translation: EAW76128.1.
BC006549 mRNA. Translation: AAH06549.1.
BC012816 mRNA. Translation: AAH12816.1.
CCDSiCCDS13278.1. [Q9GZN2-1]
PIRiJC7392.
RefSeqiNP_001186442.1. NM_001199513.1. [Q9GZN2-1]
NP_001186443.1. NM_001199514.1. [Q9GZN2-1]
NP_001186444.1. NM_001199515.1. [Q9GZN2-1]
NP_068581.1. NM_021809.6. [Q9GZN2-1]
UniGeneiHs.632264.

Genome annotation databases

EnsembliENST00000373872; ENSP00000362979; ENSG00000118707. [Q9GZN2-1]
ENST00000373874; ENSP00000362981; ENSG00000118707. [Q9GZN2-1]
ENST00000560025; ENSP00000453773; ENSG00000118707. [Q9GZN2-1]
ENST00000611732; ENSP00000481787; ENSG00000118707. [Q9GZN2-1]
GeneIDi60436.
KEGGihsa:60436.
UCSCiuc002xfn.4. human. [Q9GZN2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042646 mRNA. Translation: BAB16424.1.
BX377269 mRNA. No translation available.
AK074802 mRNA. Translation: BAC11216.1.
AK313919 mRNA. Translation: BAG36640.1.
AL050318 Genomic DNA. Translation: CAB75368.1.
CH471077 Genomic DNA. Translation: EAW76127.1.
CH471077 Genomic DNA. Translation: EAW76128.1.
BC006549 mRNA. Translation: AAH06549.1.
BC012816 mRNA. Translation: AAH12816.1.
CCDSiCCDS13278.1. [Q9GZN2-1]
PIRiJC7392.
RefSeqiNP_001186442.1. NM_001199513.1. [Q9GZN2-1]
NP_001186443.1. NM_001199514.1. [Q9GZN2-1]
NP_001186444.1. NM_001199515.1. [Q9GZN2-1]
NP_068581.1. NM_021809.6. [Q9GZN2-1]
UniGeneiHs.632264.

3D structure databases

ProteinModelPortaliQ9GZN2.
SMRiQ9GZN2. Positions 25-102.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121908. 5 interactions.
1529332. 1 interaction.
STRINGi9606.ENSP00000362979.

PTM databases

iPTMnetiQ9GZN2.
PhosphoSiteiQ9GZN2.

Polymorphism and mutation databases

BioMutaiTGIF2.
DMDMi27805750.

Proteomic databases

EPDiQ9GZN2.
MaxQBiQ9GZN2.
PaxDbiQ9GZN2.
PeptideAtlasiQ9GZN2.
PRIDEiQ9GZN2.

Protocols and materials databases

DNASUi60436.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373872; ENSP00000362979; ENSG00000118707. [Q9GZN2-1]
ENST00000373874; ENSP00000362981; ENSG00000118707. [Q9GZN2-1]
ENST00000560025; ENSP00000453773; ENSG00000118707. [Q9GZN2-1]
ENST00000611732; ENSP00000481787; ENSG00000118707. [Q9GZN2-1]
GeneIDi60436.
KEGGihsa:60436.
UCSCiuc002xfn.4. human. [Q9GZN2-1]

Organism-specific databases

CTDi60436.
GeneCardsiTGIF2.
HGNCiHGNC:15764. TGIF2.
HPAiHPA053786.
MIMi607294. gene.
neXtProtiNX_Q9GZN2.
PharmGKBiPA38035.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0773. Eukaryota.
ENOG410XPMQ. LUCA.
GeneTreeiENSGT00390000004786.
ENSGT00550000074260.
HOGENOMiHOG000232039.
HOVERGENiHBG001143.
InParanoidiQ9GZN2.
KOiK19553.
OMAiFPQGELE.
OrthoDBiEOG091G0N1F.
PhylomeDBiQ9GZN2.
TreeFamiTF314558.
TF318093.

Enzyme and pathway databases

ReactomeiR-HSA-2173795. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
R-HSA-2173796. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.

Miscellaneous databases

ChiTaRSiTGIF2. human.
GeneWikiiTGIF2.
GenomeRNAii60436.
PROiQ9GZN2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000118707.
CleanExiHS_TGIF2.
ExpressionAtlasiQ9GZN2. baseline and differential.
GenevisibleiQ9GZN2. HS.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF05920. Homeobox_KN. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTGIF2_HUMAN
AccessioniPrimary (citable) accession number: Q9GZN2
Secondary accession number(s): B2R9U3, E1P5T9, H0YNI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

TGIF2 is amplified and overexpressed in several ovarian cancer cell lines.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.