ID   RPB1B_HUMAN             Reviewed;         115 AA.
AC   Q9GZM3; F6W009; Q9H1A8;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 2.
DT   27-MAR-2024, entry version 166.
DE   RecName: Full=DNA-directed RNA polymerase II subunit RPB11-b1;
DE            Short=RNA polymerase II subunit B11-b1;
DE            Short=RPB11b1;
DE   AltName: Full=DNA-directed RNA polymerase II subunit J2;
GN   Name=POLR2J2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE
RP   SPECIFICITY, AND VARIANT ASN-28.
RC   TISSUE=Cervix carcinoma, and Placenta;
RX   PubMed=11747469; DOI=10.1186/1471-2199-2-14;
RA   Grandemange S., Schaller S., Yamano S., Du Manoir S., Shpakovski G.V.,
RA   Mattei M.-G., Kedinger C., Vigneron M.;
RT   "A human RNA polymerase II subunit is encoded by a recently generated
RT   multigene family.";
RL   BMC Mol. Biol. 2:14-14(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12853948; DOI=10.1038/nature01782;
RA   Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA   Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA   Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA   Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA   Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA   Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA   Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA   Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA   Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA   Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA   Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA   Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA   Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA   Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA   Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA   Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA   Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA   McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA   Wilson R.K.;
RT   "The DNA sequence of human chromosome 7.";
RL   Nature 424:157-164(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT ASN-28.
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   INTERACTION WITH SATB1.
RX   PubMed=12036295; DOI=10.1006/geno.2002.6772;
RA   Durrin L.K., Krontiris T.G.;
RT   "The thymocyte-specific MAR binding protein, SATB1, interacts in vitro with
RT   a novel variant of DNA-directed RNA polymerase II, subunit 11.";
RL   Genomics 79:809-817(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       Component of RNA polymerase II which synthesizes mRNA precursors and
CC       many functional non-coding RNAs. Pol II is the central component of the
CC       basal RNA polymerase II transcription machinery. It is composed of
CC       mobile elements that move relative to each other. RPB11 is part of the
CC       core element with the central large cleft (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the RNA polymerase II (Pol II) complex consisting
CC       of 12 subunits.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1; Synonyms=RPB11b1-alpha;
CC         IsoId=Q9GZM3-1; Sequence=Displayed;
CC       Name=2; Synonyms=RPB11b1-beta;
CC         IsoId=Q9GZM3-2; Sequence=VSP_030820;
CC   -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC       {ECO:0000269|PubMed:11747469}.
CC   -!- SIMILARITY: Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA
CC       polymerase subunit family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ277931; CAC18332.1; -; Genomic_DNA.
DR   EMBL; AJ277739; CAC18329.1; -; mRNA.
DR   EMBL; AJ277740; CAC18330.1; -; mRNA.
DR   EMBL; AC105052; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC086857; AAH86857.1; -; mRNA.
DR   CCDS; CCDS43627.1; -. [Q9GZM3-1]
DR   RefSeq; NP_001091084.2; NM_001097615.2.
DR   RefSeq; NP_116581.3; NM_032959.5. [Q9GZM3-1]
DR   RefSeq; XP_016867861.1; XM_017012372.1.
DR   RefSeq; XP_016867862.1; XM_017012373.1.
DR   AlphaFoldDB; Q9GZM3; -.
DR   SMR; Q9GZM3; -.
DR   BioGRID; 128910; 3.
DR   BioGRID; 139239; 36.
DR   IntAct; Q9GZM3; 1.
DR   MINT; Q9GZM3; -.
DR   STRING; 9606.ENSP00000330898; -.
DR   iPTMnet; Q9GZM3; -.
DR   PhosphoSitePlus; Q9GZM3; -.
DR   BioMuta; POLR2J2; -.
DR   jPOST; Q9GZM3; -.
DR   MassIVE; Q9GZM3; -.
DR   MaxQB; Q9GZM3; -.
DR   PaxDb; 9606-ENSP00000330898; -.
DR   PeptideAtlas; Q9GZM3; -.
DR   ProteomicsDB; 28095; -.
DR   ProteomicsDB; 80086; -. [Q9GZM3-2]
DR   Pumba; Q9GZM3; -.
DR   Antibodypedia; 57740; 101 antibodies from 12 providers.
DR   DNASU; 246721; -.
DR   Ensembl; ENST00000333432.9; ENSP00000330898.7; ENSG00000228049.8. [Q9GZM3-1]
DR   GeneID; 246721; -.
DR   GeneID; 548644; -.
DR   KEGG; hsa:246721; -.
DR   KEGG; hsa:548644; -.
DR   MANE-Select; ENST00000333432.9; ENSP00000330898.7; NM_032959.7; NP_116581.3.
DR   UCSC; uc003vah.4; human. [Q9GZM3-1]
DR   AGR; HGNC:23208; -.
DR   AGR; HGNC:33853; -.
DR   CTD; 246721; -.
DR   CTD; 548644; -.
DR   GeneCards; POLR2J2; -.
DR   HGNC; HGNC:23208; POLR2J2.
DR   HPA; ENSG00000228049; Low tissue specificity.
DR   MIM; 609881; gene.
DR   neXtProt; NX_Q9GZM3; -.
DR   PharmGKB; PA164724655; -.
DR   VEuPathDB; HostDB:ENSG00000228049; -.
DR   eggNOG; KOG4392; Eukaryota.
DR   GeneTree; ENSGT00550000074975; -.
DR   HOGENOM; CLU_090381_2_2_1; -.
DR   InParanoid; Q9GZM3; -.
DR   OMA; KLGHPNV; -.
DR   OrthoDB; 5472455at2759; -.
DR   TreeFam; TF103044; -.
DR   PathwayCommons; Q9GZM3; -.
DR   SIGNOR; Q9GZM3; -.
DR   BioGRID-ORCS; 246721; 49 hits in 357 CRISPR screens.
DR   BioGRID-ORCS; 548644; 83 hits in 320 CRISPR screens.
DR   ChiTaRS; POLR2J2; human.
DR   GeneWiki; POLR2J2; -.
DR   Pharos; Q9GZM3; Tdark.
DR   PRO; PR:Q9GZM3; -.
DR   Proteomes; UP000005640; Chromosome 7.
DR   RNAct; Q9GZM3; Protein.
DR   Bgee; ENSG00000228049; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 98 other cell types or tissues.
DR   ExpressionAtlas; Q9GZM3; baseline and differential.
DR   GO; GO:0005665; C:RNA polymerase II, core complex; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0001055; F:RNA polymerase II activity; IEA:InterPro.
DR   CDD; cd06926; RNAP_II_RPB11; 1.
DR   Gene3D; 3.30.1360.10; RNA polymerase, RBP11-like subunit; 1.
DR   HAMAP; MF_00261; RNApol_arch_Rpo11; 1.
DR   InterPro; IPR037685; RBP11.
DR   InterPro; IPR036603; RBP11-like.
DR   InterPro; IPR009025; RBP11-like_dimer.
DR   InterPro; IPR008193; RNA_pol_Rpb11_13-16kDa_CS.
DR   InterPro; IPR022905; Rpo11-like.
DR   PANTHER; PTHR13946; DNA-DIRECTED RNA POLYMERASE I,II,III; 1.
DR   PANTHER; PTHR13946:SF46; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11-A-RELATED; 1.
DR   Pfam; PF13656; RNA_pol_L_2; 1.
DR   SUPFAM; SSF55257; RBP11-like subunits of RNA polymerase; 1.
DR   PROSITE; PS01154; RNA_POL_L_13KD; 1.
DR   Genevisible; Q9GZM3; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; DNA-directed RNA polymerase; Nucleus;
KW   Reference proteome; Transcription.
FT   CHAIN           1..115
FT                   /note="DNA-directed RNA polymerase II subunit RPB11-b1"
FT                   /id="PRO_0000316946"
FT   VAR_SEQ         48..115
FT                   /note="QLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSL
FT                   LEERFRTCLLPLRLLP -> RACFPFAFCRDCQFPEASPATLPVQPAELCPRAHQLCAP
FT                   ALKRHLGGEAHPVHLHAGAASRPVQQPQHL (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:11747469,
FT                   ECO:0000303|PubMed:15489334"
FT                   /id="VSP_030820"
FT   VARIANT         28
FT                   /note="K -> N (in dbSNP:rs62483491)"
FT                   /evidence="ECO:0000269|PubMed:11747469,
FT                   ECO:0000269|PubMed:15489334"
FT                   /id="VAR_070807"
SQ   SEQUENCE   115 AA;  13088 MW;  05310B30C071AD1E CRC64;
     MNAPPAFESF LLFEGEKITI NKDTKVPKAC LFTINKEDHT LGNIIKSQLL KDPQVLFAGY
     KVPHPLEHKI IIRVQTTPDY SPQEAFTNAI TDLISELSLL EERFRTCLLP LRLLP
//