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Q9GZM3 (RPB1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase II subunit RPB11-b1

Short name=RNA polymerase II subunit B11-b1
Short name=RPB11b1
Alternative name(s):
DNA-directed RNA polymerase II subunit J2
Gene names
Name:POLR2J2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length115 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft By similarity.

Subunit structure

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.

Subcellular location

Nucleus By similarity.

Tissue specificity

Ubiquitously expressed. Ref.1

Sequence similarities

Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
Nucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: InterPro

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9GZM3-1)

Also known as: RPB11b1-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9GZM3-2)

Also known as: RPB11b1-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     48-115: QLLKDPQVLF...TCLLPLRLLP → RACFPFAFCR...SRPVQQPQHL

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 115115DNA-directed RNA polymerase II subunit RPB11-b1
PRO_0000316946

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Natural variations

Alternative sequence48 – 11568QLLKD…LRLLP → RACFPFAFCRDCQFPEASPA TLPVQPAELCPRAHQLCAPA LKRHLGGEAHPVHLHAGAAS RPVQQPQHL in isoform 2.
VSP_030820
Natural variant281K → N. Ref.1 Ref.3
Corresponds to variant rs74556368 [ dbSNP | Ensembl ].
VAR_070807

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (RPB11b1-alpha) [UniParc].

Last modified April 16, 2014. Version 2.
Checksum: 05310B30C071AD1E

FASTA11513,088
        10         20         30         40         50         60 
MNAPPAFESF LLFEGEKITI NKDTKVPKAC LFTINKEDHT LGNIIKSQLL KDPQVLFAGY 

        70         80         90        100        110 
KVPHPLEHKI IIRVQTTPDY SPQEAFTNAI TDLISELSLL EERFRTCLLP LRLLP 

« Hide

Isoform 2 (RPB11b1-beta) [UniParc].

Checksum: FD0CDC23780535EA
Show »

FASTA11612,733

References

« Hide 'large scale' references
[1]"A human RNA polymerase II subunit is encoded by a recently generated multigene family."
Grandemange S., Schaller S., Yamano S., Du Manoir S., Shpakovski G.V., Mattei M.-G., Kedinger C., Vigneron M.
BMC Mol. Biol. 2:14-14(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, VARIANT ASN-28.
Tissue: Cervix carcinoma and Placenta.
[2]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ASN-28.
Tissue: Lung.
[4]"The thymocyte-specific MAR binding protein, SATB1, interacts in vitro with a novel variant of DNA-directed RNA polymerase II, subunit 11."
Durrin L.K., Krontiris T.G.
Genomics 79:809-817(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SATB1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ277931 Genomic DNA. Translation: CAC18332.1.
AJ277739 mRNA. Translation: CAC18329.1.
AJ277740 mRNA. Translation: CAC18330.1.
AC105052 Genomic DNA. No translation available.
BC086857 mRNA. Translation: AAH86857.1.
RefSeqNP_001091084.2. NM_001097615.2.
UniGeneHs.610255.
Hs.656696.
Hs.700609.
Hs.732239.

3D structure databases

ProteinModelPortalQ9GZM3.
SMRQ9GZM3. Positions 1-106.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid139239. 1 interaction.
STRING9606.ENSP00000402065.

PTM databases

PhosphoSiteQ9GZM3.

Proteomic databases

PaxDbQ9GZM3.
PRIDEQ9GZM3.

Protocols and materials databases

DNASU548644.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000476151; ENSP00000418603; ENSG00000267645.
ENST00000591000; ENSP00000466522; ENSG00000228049.
GeneID548644.
KEGGhsa:548644.
UCSCuc011kkw.1. human. [Q9GZM3-1]

Organism-specific databases

CTD548644.
GeneCardsGC07M102282.
HGNCHGNC:23208. POLR2J2.
HPAHPA044010.
HPA046970.
MIM609881. gene.
neXtProtNX_Q9GZM3.
PharmGKBPA164724655.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1761.
HOGENOMHOG000179862.
HOVERGENHBG073419.
KOK03008.

Gene expression databases

BgeeQ9GZM3.
GenevestigatorQ9GZM3.

Family and domain databases

InterProIPR009025. RBP11-like_dimer.
IPR008193. RNA_pol_Rpb11_13-16kDa_CS.
[Graphical view]
SUPFAMSSF55257. SSF55257. 1 hit.
PROSITEPS01154. RNA_POL_L_13KD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi548644.
NextBio112313.
PROQ9GZM3.
SOURCESearch...

Entry information

Entry nameRPB1B_HUMAN
AccessionPrimary (citable) accession number: Q9GZM3
Secondary accession number(s): F6W009, Q9H1A8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: April 16, 2014
Last modified: April 16, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM