Q9GZM3 (RPB1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase II subunit RPB11-b1
Short name=RNA polymerase II subunit B11-b1
Short name=RPB11b1

Alternative name(s):
DNA-directed RNA polymerase II subunit J2

Gene names

Name:

POLR2J2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	115 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft By similarity.
Subunit structure	Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.
Subcellular location	Nucleus By similarity.
Tissue specificity	Ubiquitously expressed. Ref.1
Sequence similarities	Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9GZM3-1)

Also known as: RPB11b1-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9GZM3-2)

Also known as: RPB11b1-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     48-115: QLLKDPQVLF...TCLLPLRLLP → RACFPFAFCR...SRPVQQPQHL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 115	115	DNA-directed RNA polymerase II subunit RPB11-b1		PRO_0000316946
Natural variations
	Alternative sequence	48 – 115	68	QLLKD…LRLLP → RACFPFAFCRDCQFPEASPA TLPVQPAELCPRAHQLCAPA LKRHLGGEAHPVHLHAGAAS RPVQQPQHL in isoform 2.		VSP_030820

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (RPB11b1-alpha) [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F0345B65C524AD18	FASTA	115	13,074
	10 20 30 40 50 60 MNAPPAFESF LLFEGEKITI NKDTKVPNAC LFTINKEDHT LGNIIKSQLL KDPQVLFAGY 70 80 90 100 110 KVPHPLEHKI IIRVQTTPDY SPQEAFTNAI TDLISELSLL EERFRTCLLP LRLLP
	Isoform 2 (RPB11b1-beta) [UniParc]. Checksum: F859D9732D0060EA	FASTA	116	12,719
	10 20 30 40 50 60 MNAPPAFESF LLFEGEKITI NKDTKVPNAC LFTINKEDHT LGNIIKSRAC FPFAFCRDCQ 70 80 90 100 110 FPEASPATLP VQPAELCPRA HQLCAPALKR HLGGEAHPVH LHAGAASRPV QQPQHL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A human RNA polymerase II subunit is encoded by a recently generated multigene family." Grandemange S., Schaller S., Yamano S., Du Manoir S., Shpakovski G.V., Mattei M.-G., Kedinger C., Vigneron M. BMC Mol. Biol. 2:14-14(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY. Tissue: Cervix carcinoma and Placenta.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung.
[3]	"The thymocyte-specific MAR binding protein, SATB1, interacts in vitro with a novel variant of DNA-directed RNA polymerase II, subunit 11." Durrin L.K., Krontiris T.G. Genomics 79:809-817(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SATB1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ277931 Genomic DNA. Translation: CAC18332.1. AJ277739 mRNA. Translation: CAC18329.1. AJ277740 mRNA. Translation: CAC18330.1. BC086857 mRNA. Translation: AAH86857.1.
IPI	IPI00016841. IPI00884938.
RefSeq	NP_001091084.2. NM_001097615.2.
UniGene	Hs.610255. Hs.656696. Hs.700609. Hs.732239.
3D structure databases
ProteinModelPortal	Q9GZM3.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000402065.
PTM databases
PhosphoSite	Q9GZM3.
Polymorphism databases
DMDM	74725005.
Proteomic databases
PaxDb	Q9GZM3.
Protocols and materials databases
DNASU	548644.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	548644.
KEGG	hsa:548644.
UCSC	uc011kkw.1. human.
Organism-specific databases
CTD	548644.
GeneCards	GC07M102179.
HGNC	HGNC:23208. POLR2J2.
HPA	HPA044010. HPA046970.
MIM	609881. gene.
neXtProt	NX_Q9GZM3.
PharmGKB	PA164724655.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1761.
HOGENOM	HOG000179862.
HOVERGEN	HBG073419.
KO	K03008.
Gene expression databases
ArrayExpress	Q9GZM3.
Bgee	Q9GZM3.
Genevestigator	Q9GZM3.
Family and domain databases
InterPro	IPR009025. DNA-dir_RNA_pol_RBP11-like. IPR008193. RNA_pol_Rpb11_13-16kDa_CS. [Graphical view]
SUPFAM	SSF55257. RNAP_RBP11-like. 1 hit.
PROSITE	PS01154. RNA_POL_L_13KD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	548644.
NextBio	112313.
SOURCE	Search...

Entry information

Entry name

RPB1B_HUMAN

Accession

Primary (citable) accession number: Q9GZM3
Secondary accession number(s): Q9H1A8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.