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Protein

Ribosome biogenesis protein WDR12

Gene

WDR12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.UniRule annotation2 Publications

GO - Molecular functioni

GO - Biological processi

  • cell proliferation Source: UniProtKB
  • maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB
  • Notch signaling pathway Source: Ensembl
  • ribosomal large subunit biogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

SignaLinkiQ9GZL7.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome biogenesis protein WDR12UniRule annotation
Alternative name(s):
WD repeat-containing protein 12UniRule annotation
Gene namesi
Name:WDR12UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:14098. WDR12.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: UniProtKB
  • nucleoplasm Source: UniProtKB
  • PeBoW complex Source: UniProtKB
  • preribosome, large subunit precursor Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37841.

Polymorphism and mutation databases

BioMutaiWDR12.
DMDMi20140802.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 423422Ribosome biogenesis protein WDR12PRO_0000051358Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9GZL7.
PaxDbiQ9GZL7.
PeptideAtlasiQ9GZL7.
PRIDEiQ9GZL7.

2D gel databases

SWISS-2DPAGEQ9GZL7.

PTM databases

PhosphoSiteiQ9GZL7.

Expressioni

Inductioni

By MYC.1 Publication

Gene expression databases

BgeeiQ9GZL7.
CleanExiHS_WDR12.
ExpressionAtlasiQ9GZL7. baseline and differential.
GenevisibleiQ9GZL7. HS.

Organism-specific databases

HPAiHPA036389.

Interactioni

Subunit structurei

Component of the PeBoW complex, composed of BOP1, PES1 and WDR12. Within the PeBoW complex BOP1 interacts directly with PES1 and WDR12. The PeBoW complex also associates with the 66S pre-ribosome.UniRule annotation4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BOP1Q141373EBI-2490660,EBI-1050828

Protein-protein interaction databases

BioGridi120877. 40 interactions.
IntActiQ9GZL7. 4 interactions.
MINTiMINT-3065035.
STRINGi9606.ENSP00000261015.

Structurei

3D structure databases

ProteinModelPortaliQ9GZL7.
SMRiQ9GZL7. Positions 6-415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati99 – 13739WD 1Add
BLAST
Repeati138 – 18043WD 2Add
BLAST
Repeati187 – 22640WD 3Add
BLAST
Repeati255 – 29339WD 4Add
BLAST
Repeati295 – 33440WD 5Add
BLAST
Repeati340 – 38041WD 6Add
BLAST
Repeati384 – 42239WD 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni98 – 423326Sufficient for nucleolar localizationAdd
BLAST

Sequence similaritiesi

Belongs to the WD repeat WDR12/YTM1 family.UniRule annotation
Contains 7 WD repeats.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00780000121967.
HOGENOMiHOG000264464.
HOVERGENiHBG054155.
InParanoidiQ9GZL7.
KOiK14863.
OMAiWVTAVKW.
OrthoDBiEOG7Q2N5C.
PhylomeDBiQ9GZL7.
TreeFamiTF313023.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03029. WDR12.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR012972. NLE.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR028599. WDR12/Ytm1.
[Graphical view]
PfamiPF08154. NLE. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9GZL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLQTRFYT DNKKYAVDDV PFSIPAASEI ADLSNIINKL LKDKNEFHKH
60 70 80 90 100
VEFDFLIKGQ FLRMPLDKHM EMENISSEEV VEIEYVEKYT APQPEQCMFH
110 120 130 140 150
DDWISSIKGA EEWILTGSYD KTSRIWSLEG KSIMTIVGHT DVVKDVAWVK
160 170 180 190 200
KDSLSCLLLS ASMDQTILLW EWNVERNKVK ALHCCRGHAG SVDSIAVDGS
210 220 230 240 250
GTKFCSGSWD KMLKIWSTVP TDEEDEMEES TNRPRKKQKT EQLGLTRTPI
260 270 280 290 300
VTLSGHMEAV SSVLWSDAEE ICSASWDHTI RVWDVESGSL KSTLTGNKVF
310 320 330 340 350
NCISYSPLCK RLASGSTDRH IRLWDPRTKD GSLVSLSLTS HTGWVTSVKW
360 370 380 390 400
SPTHEQQLIS GSLDNIVKLW DTRSCKAPLY DLAAHEDKVL SVDWTDTGLL
410 420
LSGGADNKLY SYRYSPTTSH VGA
Length:423
Mass (Da):47,708
Last modified:March 27, 2002 - v2
Checksum:i6D088C640AC981D8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti333 – 3331L → M in AAF60355 (Ref. 1) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti72 – 721M → V.
VAR_012863
Natural varianti75 – 751I → V.1 Publication
Corresponds to variant rs35212307 [ dbSNP | Ensembl ].
VAR_054888
Natural varianti89 – 891Y → C.
VAR_012864
Natural varianti286 – 2861E → G.
VAR_012865

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242546 mRNA. Translation: AAF60355.1.
AK001743 mRNA. Translation: BAA91875.1.
AK022781 mRNA. Translation: BAB14242.1.
AK022782 mRNA. Translation: BAB14243.1.
AK056092 mRNA. Translation: BAG51621.1.
BC008082 mRNA. Translation: AAH08082.1.
CCDSiCCDS2356.1.
RefSeqiNP_060726.3. NM_018256.3.
UniGeneiHs.73291.

Genome annotation databases

EnsembliENST00000261015; ENSP00000261015; ENSG00000138442.
GeneIDi55759.
KEGGihsa:55759.
UCSCiuc002uzl.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242546 mRNA. Translation: AAF60355.1.
AK001743 mRNA. Translation: BAA91875.1.
AK022781 mRNA. Translation: BAB14242.1.
AK022782 mRNA. Translation: BAB14243.1.
AK056092 mRNA. Translation: BAG51621.1.
BC008082 mRNA. Translation: AAH08082.1.
CCDSiCCDS2356.1.
RefSeqiNP_060726.3. NM_018256.3.
UniGeneiHs.73291.

3D structure databases

ProteinModelPortaliQ9GZL7.
SMRiQ9GZL7. Positions 6-415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120877. 40 interactions.
IntActiQ9GZL7. 4 interactions.
MINTiMINT-3065035.
STRINGi9606.ENSP00000261015.

PTM databases

PhosphoSiteiQ9GZL7.

Polymorphism and mutation databases

BioMutaiWDR12.
DMDMi20140802.

2D gel databases

SWISS-2DPAGEQ9GZL7.

Proteomic databases

MaxQBiQ9GZL7.
PaxDbiQ9GZL7.
PeptideAtlasiQ9GZL7.
PRIDEiQ9GZL7.

Protocols and materials databases

DNASUi55759.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261015; ENSP00000261015; ENSG00000138442.
GeneIDi55759.
KEGGihsa:55759.
UCSCiuc002uzl.3. human.

Organism-specific databases

CTDi55759.
GeneCardsiGC02M203709.
HGNCiHGNC:14098. WDR12.
HPAiHPA036389.
neXtProtiNX_Q9GZL7.
PharmGKBiPA37841.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00780000121967.
HOGENOMiHOG000264464.
HOVERGENiHBG054155.
InParanoidiQ9GZL7.
KOiK14863.
OMAiWVTAVKW.
OrthoDBiEOG7Q2N5C.
PhylomeDBiQ9GZL7.
TreeFamiTF313023.

Enzyme and pathway databases

SignaLinkiQ9GZL7.

Miscellaneous databases

ChiTaRSiWDR12. human.
GeneWikiiWDR12.
GenomeRNAii55759.
NextBioi60775.
PROiQ9GZL7.

Gene expression databases

BgeeiQ9GZL7.
CleanExiHS_WDR12.
ExpressionAtlasiQ9GZL7. baseline and differential.
GenevisibleiQ9GZL7. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03029. WDR12.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR012972. NLE.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR028599. WDR12/Ytm1.
[Graphical view]
PfamiPF08154. NLE. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human homolog of Saccharomyces cervisiae YTM1."
    Matsumoto S.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-75.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skin.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation."
    Hoelzel M., Rohrmoser M., Schlee M., Grimm T., Harasim T., Malamoussi A., Gruber-Eber A., Kremmer E., Hiddemann W., Bornkamm G.W., Eick D.
    J. Cell Biol. 170:367-378(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BOP1 AND PES1, SUBCELLULAR LOCATION, INDUCTION.
  6. "Dominant-negative Pes1 mutants inhibit ribosomal RNA processing and cell proliferation via incorporation into the PeBoW-complex."
    Grimm T., Hoelzel M., Rohrmoser M., Harasim T., Malamoussi A., Gruber-Eber A., Kremmer E., Eick D.
    Nucleic Acids Res. 34:3030-3043(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PES1 AND WDR12.
  7. "Interdependence of Pes1, Bop1, and WDR12 controls nucleolar localization and assembly of the PeBoW complex required for maturation of the 60S ribosomal subunit."
    Rohrmoser M., Hoelzel M., Grimm T., Malamoussi A., Harasim T., Orban M., Pfisterer I., Gruber-Eber A., Kremmer E., Eick D.
    Mol. Cell. Biol. 27:3682-3694(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BOP1 AND PES1.
  8. "The BRCT domain of mammalian Pes1 is crucial for nucleolar localization and rRNA processing."
    Hoelzel M., Grimm T., Rohrmoser M., Malamoussi A., Harasim T., Gruber-Eber A., Kremmer E., Eick D.
    Nucleic Acids Res. 35:789-800(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BOP1 AND PES1.
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiWDR12_HUMAN
AccessioniPrimary (citable) accession number: Q9GZL7
Secondary accession number(s): B3KPA9
, Q96HU0, Q9NV80, Q9NYI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: June 24, 2015
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.