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Protein

Endoglycoceramidase

Gene
N/A
Organism
Cyanea nozakii (Jellyfish)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolysis of the glycosidic linkage between oligosaccharides and ceramides of glycosphingolipids, especially b-series polysialogangliosides.1 Publication

Catalytic activityi

Oligoglycosylglucosyl-(1<->1)-ceramide + H2O = ceramide + oligoglycosylglucose.1 Publication

Enzyme regulationi

Completely inhibited by Hg2+. Cu2+ and zinc have no effect on enzyme activity. Lithium, potassium, manganese, Ni2+, calcium, magnesium and EDTA have no significant effect on enzyme activity. Enzyme requires presence of detergents such as Triton X-100 and Lubrol PX for the hydrolysis of glycosphingolipids. Taurodeoxycholate strongly inhibits the enzyme activity and SDS completely inhibits the enzyme activity.1 Publication

Kineticsi

  1. KM=0.35 mM for GM11 Publication
  1. Vmax=4.4 µM/min/mg enzyme1 Publication

pH dependencei

Optimum pH 3.0. Highly stable at acidic pH.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei239Proton donorSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BRENDAi3.2.1.123 1767
SABIO-RKQ9GV16

Protein family/group databases

CAZyiGH5 Glycoside Hydrolase Family 5

Names & Taxonomyi

Protein namesi
Recommended name:
EndoglycoceramidaseImported (EC:3.2.1.123)
Short name:
EGCase1 Publication
Alternative name(s):
Glycosphingolipid-specific enzyme1 Publication
Short name:
GSL-specific enzyme1 Publication
OrganismiCyanea nozakii (Jellyfish)
Taxonomic identifieri135523 [NCBI]
Taxonomic lineageiEukaryotaMetazoaCnidariaScyphozoaSemaeostomeaeCyaneidaeCyanea

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nematocyst, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000039038122 – 503EndoglycoceramidaseSequence analysisAdd BLAST482

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi72N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi108N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi205N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi307N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi409N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi485N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ9GV16

PTM databases

iPTMnetiQ9GV16

Structurei

3D structure databases

ProteinModelPortaliQ9GV16
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 5 (cellulase A) family.Sequence analysis

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.1180, 2 hits
InterProiView protein in InterPro
IPR001547 Glyco_hydro_5
IPR018087 Glyco_hydro_5_CS
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00150 Cellulase, 1 hit
SUPFAMiSSF51445 SSF51445, 2 hits
PROSITEiView protein in PROSITE
PS00659 GLYCOSYL_HYDROL_F5, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9GV16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETQPLVFV LMSISAILTA GLPINDDASL LISVNPETQQ LVDSLGRERF
60 70 80 90 100
FHGTNVVVKH KPYHPSVEGY DNTSFSEVDM KILQDLGLNT IRLGMMLPGY
110 120 130 140 150
VPTRGNYNET YLKIIQEIVS KAAKYGIYTL LDMHQDVMSA KFCVEGFPDW
160 170 180 190 200
AVNTGNADNF PFPLEDKYPL NLQTGYPYPK DCAKHAWGDY YFTEAAAAAF
210 220 230 240 250
QNFYNNTDGL LDAWADFWKK TAQGFKDYKS VIGYELINEP FAGDIYRDPS
260 270 280 290 300
LMIPGVADER NLAPAYDVIH KAIRTVDEQH SIFFEGVTWD YFAAGFSKVP
310 320 330 340 350
GGDAYRNRSV LSYHYYEPPD FNKKFQFEVR MEDLRRLKCG GFLTELLTVG
360 370 380 390 400
DTAKDMSDML ELFDICDQHK QSWMGWLYKS YGCYKQHLGC LTDSMHDETG
410 420 430 440 450
HLRDIVLQNT TRTYPQAVAG HTIGYKFDRI TKKFDLSFVV TADCRSTESI
460 470 480 490 500
VYFNKDLHYS NGYDVTVFPK DSVTWKQVEK KIIINHSQKL SAGTTVTFSL

VAK
Length:503
Mass (Da):57,253
Last modified:March 1, 2001 - v1
Checksum:i3383E64987E7B15A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti172L → P1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047321 mRNA Translation: BAB16369.1
AB047322 mRNA Translation: BAB16370.1

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiEGCSE_CYANO
AccessioniPrimary (citable) accession number: Q9GV16
Secondary accession number(s): Q9GV15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

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