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Q9GUM2 (BRE4_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,4-N-acetylgalactosaminyltransferase bre-4
EC=2.4.1.-
Alternative name(s):
Bacillus thuringiensis toxin-resistant protein 4
Short name=Bt toxin-resistant protein 4
Beta-4-GalNAcT
Gene names
Name:bre-4
ORF Names:Y73E7A.7
OrganismCaenorhabditis elegans
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length383 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the transfer of galactose onto proteins or lipids. Required for susceptibility to pore-forming crystal toxins in conjunction with bre-1, bre-2, bre-3 and bre-5. Ref.1 Ref.2 Ref.4

Cofactor

Manganese By similarity. UniProtKB P08037

Pathway

Protein modification; protein glycosylation. Ref.1 Ref.2

Subcellular location

Membrane; Single-pass type II membrane protein Potential.

Disruption phenotype

Worms exhibit resistance to the Cry5B toxin produced by Bacillus thuringiensis. This is thought to be due to mutants having reduced population of glycolipids which are targeted by the Cry5B protein. Ref.2 Ref.4

Sequence similarities

Belongs to the glycosyltransferase 7 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 383383Beta-1,4-N-acetylgalactosaminyltransferase bre-4
PRO_0000324671

Regions

Topological domain1 – 66Cytoplasmic Potential
Transmembrane7 – 2923Helical; Signal-anchor for type II membrane protein; Potential
Topological domain30 – 383354Extracellular Potential

Sites

Metal binding2221Manganese By similarity UniProtKB P08037
Metal binding3151Manganese By similarity UniProtKB P08037

Amino acid modifications

Glycosylation371N-linked (GlcNAc...) Potential
Glycosylation711N-linked (GlcNAc...) Potential
Glycosylation811N-linked (GlcNAc...) Potential
Glycosylation971N-linked (GlcNAc...) Potential
Glycosylation1911N-linked (GlcNAc...) Potential
Disulfide bond103 ↔ 144 By similarity UniProtKB P08037
Disulfide bond215 ↔ 234 By similarity UniProtKB P08037

Experimental info

Mutagenesis2601G → R in ye43; resistant to Bacillus thuringiensis crystal5B toxin. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9GUM2 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: ADEDFA4275B2CE81

FASTA38343,914
        10         20         30         40         50         60 
MAFRHLAVAR LKSLLVLCAV LLLVHAMIYK IPSLYENLTI GSSTLIADVD AMEAVLGNTA 

        70         80         90        100        110        120 
STSDDLLDTW NSTFSPISEV NQTSFMEDIR PILFPDNQTL QFCNQTPPHL VGPIRVFLDE 

       130        140        150        160        170        180 
PDFKTLEKIY PDTHAGGHGM PKDCVARHRV AIIVPYRDRE AHLRIMLHNL HSLLAKQQLD 

       190        200        210        220        230        240 
YAIFIVEQVA NQTFNRGKLM NVGYDVASRL YPWQCFIFHD VDLLPEDDRN LYTCPIQPRH 

       250        260        270        280        290        300 
MSVAIDKFNY KLPYSAIFGG ISALTKDHLK KINGFSNDFW GWGGEDDDLA TRTSMAGLKV 

       310        320        330        340        350        360 
SRYPTQIARY KMIKHSTEAT NPVNKCRYKI MGQTKRRWTR DGLSNLKYKL VNLELKPLYT 

       370        380 
RAVVDLLEKD CRRELRRDFP TCF

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and enzymatic characterization of a UDP-GalNAc:GlcNAc(beta)-R beta1,4-N-acetylgalactosaminyltransferase from Caenorhabditis elegans."
Kawar Z.S., Van Die I., Cummings R.D.
J. Biol. Chem. 277:34924-34932(2002) [PubMed: 12167666] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PATHWAY.
[2]"Resistance to a bacterial toxin is mediated by removal of a conserved glycosylation pathway required for toxin-host interactions."
Griffitts J.S., Huffman D.L., Whitacre J.L., Barrows B.D., Marroquin L.D., Mueller R., Brown J.R., Hennet T., Esko J.D., Aroian R.V.
J. Biol. Chem. 278:45594-45602(2003) [PubMed: 12944392] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF GLY-260, DISRUPTION PHENOTYPE.
[3]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[4]"Bacillus thuringiensis (Bt) toxin susceptibility and isolation of resistance mutants in the nematode Caenorhabditis elegans."
Marroquin L.D., Elyassnia D., Griffitts J.S., Feitelson J.S., Aroian R.V.
Genetics 155:1693-1699(2000) [PubMed: 10924467] [Abstract]
Cited for: IDENTIFICATION, FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY130767 mRNA. Translation: AAM95168.1.
AY533306 mRNA. Translation: AAS21308.1.
FO081821 Genomic DNA. Translation: CCD73531.1.
RefSeqNP_490872.1. NM_058471.1.
UniGeneCel.9283.

3D structure databases

HSSPHSSP built from PDB template 2FYA based on UniProtKB P15291.
ProteinModelPortalQ9GUM2.
SMRQ9GUM2. Positions 99-369.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9GUM2.

Protein family/group databases

CAZyGT7. Glycosyltransferase Family 7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaY73E7A.7; Y73E7A.7; Y73E7A.7.
GeneID190668.
KEGGcel:Y73E7A.7.
NMPDRfig|6239.3.peg.252.
UCSCY73E7A.7. c. elegans.

Organism-specific databases

CTD190668.
WormBaseY73E7A.7; CE26412; WBGene00000269; bre-4.

Phylogenomic databases

eggNOGmeNOG05687.
GeneTreeEMGT00050000004582.
HOGENOMHBG717407.
InParanoidQ9GUM2.
OMAPHLVGPI.
PhylomeDBQ9GUM2.

Gene expression databases

ArrayExpressQ9GUM2.

Family and domain databases

InterProIPR003859. Galactosyl_T_2_met.
[Graphical view]
KOK07968.
PANTHERPTHR19300. Galactosyl_T_2. 1 hit.
PfamPF02709. Galactosyl_T_2. 1 hit.
[Graphical view]
PRINTSPR02050. B14GALTRFASE.
ProtoNetSearch...

Other

NextBio946570.

Entry information

Entry nameBRE4_CAEEL
AccessionPrimary (citable) accession number: Q9GUM2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 1, 2001
Last modified: December 14, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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