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Protein

Synembryn

Gene

ric-8

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins independently of G-protein coupled receptors. Acts by exchanging bound GDP for free GTP. Able to facilitate synaptic transmission in the nervous system probably by activating G(q)-alpha (egl-30). Also able to activate the G(s)-alpha in synaptic signaling network. Plays a key role in asymmetric spindle positioning, a step for asymmetric cell division that generates cell diversity during development by activating G(i)-alpha protein goa-1 and gpa-16 independently of G-protein coupled receptors. While it acts as a GEF for goa-1, it has no GEF activity toward gpa-16. In addition to its GEF activity, it is required for cortical subcellular localization of G-alpha proteins such as gpa-16. Also required for the interaction of goa-1 and gpr-1/2, suggesting that it may act by generating G-alpha proteins free from G-beta-gamma subunits, enabling gpr-1/2 to mediate asymmetric cell division.8 Publications

GO - Molecular functioni

  • G-protein alpha-subunit binding Source: GO_Central
  • GTPase activator activity Source: WormBase
  • guanyl-nucleotide exchange factor activity Source: WormBase

GO - Biological processi

  • G-protein coupled receptor signaling pathway Source: GO_Central
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of protein kinase C signaling Source: WormBase
  • protein localization to cell cortex Source: WormBase
  • regulation of establishment of cell polarity Source: WormBase
  • regulation of feeding behavior Source: WormBase

Keywordsi

Molecular functionDevelopmental protein, Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Synembryn
Alternative name(s):
Resistance to inhibitors of cholinesterase protein 8
Gene namesi
Name:ric-8
ORF Names:Y69A2AR.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiY69A2AR.2a; CE27516; WBGene00004367; ric-8.
Y69A2AR.2b; CE33952; WBGene00004367; ric-8.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi267L → F in md1712; exhibits partial embryonic lethality that appears to result from defects in the regulation of a subset of centrosome movements during early embryogenesis. 1 Publication1
Mutagenesisi275A → E in md303; exhibits partial embryonic lethality that appears to result from defects in the regulation of a subset of centrosome movements during early embryogenesis; impairs interaction with goa-1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002359041 – 566SynembrynAdd BLAST566

Proteomic databases

EPDiQ9GSX9.
PaxDbiQ9GSX9.
PeptideAtlasiQ9GSX9.
PRIDEiQ9GSX9.

PTM databases

iPTMnetiQ9GSX9.

Expressioni

Tissue specificityi

Present throughout the nervous system in juveniles and adults (at protein level).1 Publication

Gene expression databases

BgeeiWBGene00004367.
ExpressionAtlasiQ9GSX9. baseline.

Interactioni

Subunit structurei

Interacts with GDP-bound G-alpha proteins goa-1 and gpa-16. Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma.4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi42196. 8 interactors.
DIPiDIP-26607N.
IntActiQ9GSX9. 11 interactors.
MINTiMINT-1048950.
STRINGi6239.Y69A2AR.2a.

Structurei

3D structure databases

ProteinModelPortaliQ9GSX9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the synembryn family.Curated

Phylogenomic databases

eggNOGiKOG4464. Eukaryota.
ENOG410XSCJ. LUCA.
GeneTreeiENSGT00390000014700.
HOGENOMiHOG000154125.
InParanoidiQ9GSX9.
OMAiFIICKRS.
OrthoDBiEOG091G07N6.
PhylomeDBiQ9GSX9.

Family and domain databases

InterProiView protein in InterPro
IPR019318. Gua_nucleotide_exch_fac_Ric8.
IPR008376. Synembryn.
PfamiView protein in Pfam
PF10165. Ric8. 1 hit.
PRINTSiPR01802. SYNEMBRYN.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q9GSX9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEELHSDLI ASIFGGKPAK IEEFFSKWNF ANAAVSKFDM ANSAKNELGD
60 70 80 90 100
RICEVIENGE LTHVLLETIK ILSREKDGLE GLLNDPLCDK ILAFAELSSN
110 120 130 140 150
ENNSKTVHTL MEAQKCLINT LFHSQRMRDR FYANPKTGEN LQFFLGEFEE
160 170 180 190 200
NRRKTSSIDW IRLLNPVQAA EIWYFYHRIA FIATALGREF QKNWANDPKT
210 220 230 240 250
IDSLLLAVEI CTNRSENSTQ DINRATEALK TFFNVFCHFH GDVKAIDHKN
260 270 280 290 300
AAKTCQILRD AICSDVLTDD VVQSAIHCLS VPPLPMVLSV LCGKNSKNNG
310 320 330 340 350
GENEEEKFFV EELSNMQLTE AILMHLDKQL TKVVALLLND APNQQQNPML
360 370 380 390 400
SAEASTLTDL VGPYFQVLAR LCTDSKYVRR YCRIRVIPPL VSEEVQKRPE
410 420 430 440 450
ENNTLRGRIA RIMMLPSSTK DVAAEFLFII CKRSVNRMIK YLGFGHSAGH
460 470 480 490 500
LANLGLLGQI NQPKHASDSE DSETEDYNQI KDSVNPVTGA IYPSDHGSAL
510 520 530 540 550
AGMSEEQKEY EAMKLVDAMN QMMETGIVKP GTIGDDGKIR EVSHVLELLK
560
NAPEPAPAEN SDSDEE
Length:566
Mass (Da):63,447
Last modified:March 1, 2001 - v1
Checksum:i10C4E5423BB81FBF
GO
Isoform b (identifier: Q9GSX9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     109-157: TLMEAQKCLI...FEENRRKTSS → SKAILSLKIL...RSILCKPENW
     158-566: Missing.

Note: No experimental confirmation available.
Show »
Length:157
Mass (Da):17,438
Checksum:i7A61D0D2EE8F6325
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018516109 – 157TLMEA…RKTSS → SKAILSLKILEFPAKNHNFS TNGGAKMSNQHFVSLTTNER SILCKPENW in isoform b. CuratedAdd BLAST49
Alternative sequenceiVSP_018517158 – 566Missing in isoform b. CuratedAdd BLAST409

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288812 mRNA. Translation: AAG10199.1.
FO081818 Genomic DNA. Translation: CCD74109.1.
FO081818 Genomic DNA. Translation: CCD74110.1.
RefSeqiNP_001023561.1. NM_001028390.2. [Q9GSX9-1]
NP_001023562.1. NM_001028391.3. [Q9GSX9-2]
UniGeneiCel.19475.

Genome annotation databases

EnsemblMetazoaiY69A2AR.2a; Y69A2AR.2a; WBGene00004367. [Q9GSX9-1]
GeneIDi177048.
KEGGicel:CELE_Y69A2AR.2.
UCSCiY69A2AR.2a. c. elegans. [Q9GSX9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRIC8_CAEEL
AccessioniPrimary (citable) accession number: Q9GSX9
Secondary accession number(s): Q86DD4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: March 1, 2001
Last modified: September 27, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families