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Protein

Synembryn

Gene

ric-8

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins independently of G-protein coupled receptors. Acts by exchanging bound GDP for free GTP. Able to facilitate synaptic transmission in the nervous system probably by activating G(q)-alpha (egl-30). Also able to activate the G(s)-alpha in synaptic signaling network. Plays a key role in asymmetric spindle positioning, a step for asymmetric cell division that generates cell diversity during development by activating G(i)-alpha protein goa-1 and gpa-16 independently of G-protein coupled receptors. While it acts as a GEF for goa-1, it has no GEF activity toward gpa-16. In addition to its GEF activity, it is required for cortical subcellular localization of G-alpha proteins such as gpa-16. Also required for the interaction of goa-1 and gpr-1/2, suggesting that it may act by generating G-alpha proteins free from G-beta-gamma subunits, enabling gpr-1/2 to mediate asymmetric cell division.8 Publications

GO - Molecular functioni

  • GTPase activator activity Source: WormBase
  • guanyl-nucleotide exchange factor activity Source: WormBase

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of protein kinase C signaling Source: WormBase
  • positive regulation of GTPase activity Source: GOC
  • protein localization to cell cortex Source: WormBase
  • regulation of establishment of cell polarity Source: WormBase
  • regulation of feeding behavior Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Synembryn
Alternative name(s):
Resistance to inhibitors of cholinesterase protein 8
Gene namesi
Name:ric-8
ORF Names:Y69A2AR.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiY69A2AR.2a; CE27516; WBGene00004367; ric-8.
Y69A2AR.2b; CE33952; WBGene00004367; ric-8.

Subcellular locationi

GO - Cellular componenti

  • aster Source: WormBase
  • cell cortex Source: WormBase
  • kinetochore microtubule Source: WormBase
  • spindle Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi267 – 2671L → F in md1712; exhibits partial embryonic lethality that appears to result from defects in the regulation of a subset of centrosome movements during early embryogenesis. 1 Publication
Mutagenesisi275 – 2751A → E in md303; exhibits partial embryonic lethality that appears to result from defects in the regulation of a subset of centrosome movements during early embryogenesis; impairs interaction with goa-1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566SynembrynPRO_0000235904Add
BLAST

Proteomic databases

EPDiQ9GSX9.
PaxDbiQ9GSX9.
PRIDEiQ9GSX9.

PTM databases

iPTMnetiQ9GSX9.

Expressioni

Tissue specificityi

Present throughout the nervous system in juveniles and adults (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ9GSX9. baseline.

Interactioni

Subunit structurei

Interacts with GDP-bound G-alpha proteins goa-1 and gpa-16. Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
goa-1P518759EBI-1004494,EBI-316062
gpa-16Q9N2V62EBI-1004494,EBI-1005005

Protein-protein interaction databases

BioGridi42196. 8 interactions.
DIPiDIP-26607N.
IntActiQ9GSX9. 6 interactions.
MINTiMINT-1048950.
STRINGi6239.Y69A2AR.2a.

Structurei

3D structure databases

ProteinModelPortaliQ9GSX9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the synembryn family.Curated

Phylogenomic databases

eggNOGiKOG4464. Eukaryota.
ENOG410XSCJ. LUCA.
GeneTreeiENSGT00390000014700.
HOGENOMiHOG000154125.
InParanoidiQ9GSX9.
OMAiEMCLINI.
OrthoDBiEOG72JWG4.
PhylomeDBiQ9GSX9.

Family and domain databases

InterProiIPR019318. Gua_nucleotide_exch_fac_Ric8.
IPR008376. Synembryn.
[Graphical view]
PfamiPF10165. Ric8. 1 hit.
[Graphical view]
PRINTSiPR01802. SYNEMBRYN.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q9GSX9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEELHSDLI ASIFGGKPAK IEEFFSKWNF ANAAVSKFDM ANSAKNELGD
60 70 80 90 100
RICEVIENGE LTHVLLETIK ILSREKDGLE GLLNDPLCDK ILAFAELSSN
110 120 130 140 150
ENNSKTVHTL MEAQKCLINT LFHSQRMRDR FYANPKTGEN LQFFLGEFEE
160 170 180 190 200
NRRKTSSIDW IRLLNPVQAA EIWYFYHRIA FIATALGREF QKNWANDPKT
210 220 230 240 250
IDSLLLAVEI CTNRSENSTQ DINRATEALK TFFNVFCHFH GDVKAIDHKN
260 270 280 290 300
AAKTCQILRD AICSDVLTDD VVQSAIHCLS VPPLPMVLSV LCGKNSKNNG
310 320 330 340 350
GENEEEKFFV EELSNMQLTE AILMHLDKQL TKVVALLLND APNQQQNPML
360 370 380 390 400
SAEASTLTDL VGPYFQVLAR LCTDSKYVRR YCRIRVIPPL VSEEVQKRPE
410 420 430 440 450
ENNTLRGRIA RIMMLPSSTK DVAAEFLFII CKRSVNRMIK YLGFGHSAGH
460 470 480 490 500
LANLGLLGQI NQPKHASDSE DSETEDYNQI KDSVNPVTGA IYPSDHGSAL
510 520 530 540 550
AGMSEEQKEY EAMKLVDAMN QMMETGIVKP GTIGDDGKIR EVSHVLELLK
560
NAPEPAPAEN SDSDEE
Length:566
Mass (Da):63,447
Last modified:March 1, 2001 - v1
Checksum:i10C4E5423BB81FBF
GO
Isoform b (identifier: Q9GSX9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     109-157: TLMEAQKCLI...FEENRRKTSS → SKAILSLKIL...RSILCKPENW
     158-566: Missing.

Note: No experimental confirmation available.
Show »
Length:157
Mass (Da):17,438
Checksum:i7A61D0D2EE8F6325
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei109 – 15749TLMEA…RKTSS → SKAILSLKILEFPAKNHNFS TNGGAKMSNQHFVSLTTNER SILCKPENW in isoform b. CuratedVSP_018516Add
BLAST
Alternative sequencei158 – 566409Missing in isoform b. CuratedVSP_018517Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288812 mRNA. Translation: AAG10199.1.
FO081818 Genomic DNA. Translation: CCD74109.1.
FO081818 Genomic DNA. Translation: CCD74110.1.
RefSeqiNP_001023561.1. NM_001028390.2. [Q9GSX9-1]
NP_001023562.1. NM_001028391.3. [Q9GSX9-2]
UniGeneiCel.19475.

Genome annotation databases

EnsemblMetazoaiY69A2AR.2a; Y69A2AR.2a; WBGene00004367. [Q9GSX9-1]
GeneIDi177048.
KEGGicel:CELE_Y69A2AR.2.
UCSCiY69A2AR.2a. c. elegans. [Q9GSX9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288812 mRNA. Translation: AAG10199.1.
FO081818 Genomic DNA. Translation: CCD74109.1.
FO081818 Genomic DNA. Translation: CCD74110.1.
RefSeqiNP_001023561.1. NM_001028390.2. [Q9GSX9-1]
NP_001023562.1. NM_001028391.3. [Q9GSX9-2]
UniGeneiCel.19475.

3D structure databases

ProteinModelPortaliQ9GSX9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi42196. 8 interactions.
DIPiDIP-26607N.
IntActiQ9GSX9. 6 interactions.
MINTiMINT-1048950.
STRINGi6239.Y69A2AR.2a.

PTM databases

iPTMnetiQ9GSX9.

Proteomic databases

EPDiQ9GSX9.
PaxDbiQ9GSX9.
PRIDEiQ9GSX9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY69A2AR.2a; Y69A2AR.2a; WBGene00004367. [Q9GSX9-1]
GeneIDi177048.
KEGGicel:CELE_Y69A2AR.2.
UCSCiY69A2AR.2a. c. elegans. [Q9GSX9-1]

Organism-specific databases

CTDi177048.
WormBaseiY69A2AR.2a; CE27516; WBGene00004367; ric-8.
Y69A2AR.2b; CE33952; WBGene00004367; ric-8.

Phylogenomic databases

eggNOGiKOG4464. Eukaryota.
ENOG410XSCJ. LUCA.
GeneTreeiENSGT00390000014700.
HOGENOMiHOG000154125.
InParanoidiQ9GSX9.
OMAiEMCLINI.
OrthoDBiEOG72JWG4.
PhylomeDBiQ9GSX9.

Miscellaneous databases

NextBioi895112.
PROiQ9GSX9.

Gene expression databases

ExpressionAtlasiQ9GSX9. baseline.

Family and domain databases

InterProiIPR019318. Gua_nucleotide_exch_fac_Ric8.
IPR008376. Synembryn.
[Graphical view]
PfamiPF10165. Ric8. 1 hit.
[Graphical view]
PRINTSiPR01802. SYNEMBRYN.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RIC-8 (Synembryn): a novel conserved protein that is required for Gq alpha signaling in the C. elegans nervous system."
    Miller K.G., Emerson M.D., McManus J.R., Rand J.B.
    Neuron 27:289-299(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LEU-267 AND ALA-275.
    Strain: Bristol N2.
  3. "A role for RIC-8 (Synembryn) and GOA-1 (G(o)alpha) in regulating a subset of centrosome movements during early embryogenesis in Caenorhabditis elegans."
    Miller K.G., Rand J.B.
    Genetics 156:1649-1660(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "RGS-7 completes a receptor-independent heterotrimeric G protein cycle to asymmetrically regulate mitotic spindle positioning in C. elegans."
    Hess H.A., Roeper J.-C., Grill S.W., Koelle M.R.
    Cell 119:209-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GOA-1.
  5. "RIC-8 is required for GPR-1/2-dependent Galpha function during asymmetric division of C. elegans embryos."
    Afshar K., Willard F.S., Colombo K., Johnston C.A., McCudden C.R., Siderovski D.P., Goenczy P.
    Cell 119:219-230(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GOA-1.
  6. "Control of embryonic spindle positioning and Galpha activity by C. elegans RIC-8."
    Couwenbergs C., Spilker A.C., Gotta M.
    Curr. Biol. 14:1871-1876(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GOA-1.
  7. "Cortical localization of the Galpha protein GPA-16 requires RIC-8 function during C. elegans asymmetric cell division."
    Afshar K., Willard F.S., Colombo K., Siderovski D.P., Goenczy P.
    Development 132:4449-4459(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GPA-16.
  8. "Mutations that rescue the paralysis of Caenorhabditis elegans ric-8 (synembryn) mutants activate the G alpha(s) pathway and define a third major branch of the synaptic signaling network."
    Schade M.A., Reynolds N.K., Dollins C.M., Miller K.G.
    Genetics 169:631-649(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Convergent, RIC-8-dependent Galpha signaling pathways in the Caenorhabditis elegans synaptic signaling network."
    Reynolds N.K., Schade M.A., Miller K.G.
    Genetics 169:651-670(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiRIC8_CAEEL
AccessioniPrimary (citable) accession number: Q9GSX9
Secondary accession number(s): Q86DD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: March 1, 2001
Last modified: May 11, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.