Reviewed, UniProtKB/Swiss-Prot Q9GQF1 (JIP3_DROME)

Names and origin

Protein names

Recommended name:
    JNK-interacting protein 3
Alternative name(s):
    Protein sunday driver

Gene names

Name:	syd
ORF Names:	CG8110

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK-signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interations with the JNK-signaling components and motor proteins. Syd is required for efficient kinesin-I mediated axonal transport. UniProtKB Q9ESN9 Ref.1
Subunit structure	Forms homo- and heterooligomeric complexes. Binds the TPR motif-containing C-terminal of kinesin light chain, Klc. Pre-assembled syd scaffolding complexes are then transported as a cargo of kinesin, to the required subcellular location.
Subcellular location	Cytoplasm By similarity. UniProtKB Q9ESN9
Sequence similarities	Belongs to the JIP scaffold family.
RNA editing	Edited at position 983. Partially edited. Target of Adar. Ref.6
Sequence caution	The sequence AAN71473.1 differs from that shown. Reason: Erroneous termination at position 795. Translated as Arg. The sequence AAN71473.1 differs from that shown. Reason: Frameshift at position 1207. The sequence AAV36895.1 differs from that shown. Reason: Frameshift at position 397. The sequence AAV36895.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing RNA editing
Domain	Coiled coil
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	axon cargo transport Ref.1 Inferred from mutant phenotype. Source: FlyBase regulation of JNK cascade Inferred from sequence or structural similarity. Source: UniProtKB vesicle-mediated transport Ref.1 Inferred from expression pattern. Source: UniProtKB
Cellular component	Golgi membrane Inferred from sequence or structural similarity. Source: UniProtKB trans-Golgi network transport vesicle Ref.1 Inferred from direct assay. Source: FlyBase
Molecular function	MAP-kinase scaffold activity Inferred from sequence or structural similarity. Source: UniProtKB kinesin binding Ref.1 Inferred from physical interaction. Source: UniProtKB protein kinase binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9GQF1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q9GQF1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: MMDNDDALLNNGGPQSGAETVYGTEDNNMVMSEK → M

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1227	1227	JNK-interacting protein 3		PRO_0000220636
Regions
	Coiled coil	84 – 191	108	Potential
	Coiled coil	363 – 489	127	Potential
	Coiled coil	814 – 849	36	Potential
Natural variations
	Alternative sequence	1 – 34	34	MMDND…VMSEK → M in isoform B.		VSP_002780
	Natural variant	983	1	S → G in RNA edited version.
Experimental info
	Sequence conflict	355 – 360	6	Missing in AAN71473. Ref.4
	Sequence conflict	792	1	M → I in AAN71473. Ref.4
	Sequence conflict	1124	1	K → R in AAN71473. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 2162C18066A057B8	FASTA	1,227	136,712
	10 20 30 40 50 60 MMDNDDALLN NGGPQSGAET VYGTEDNNMV MSEKNEQVVS IVQQLAGSIY QEFERMINRY 70 80 90 100 110 120 DEDVVKNLMP LLVNVLECLD ASYRINQEQD VEVELLREDN EQLVTQYERE KSARKQSEQK 130 140 150 160 170 180 LLEAEDLAEQ ENKELATRLE SVESIVRMLE LKHKNSLEHA SRLEEREADL KKEYNKLHER 190 200 210 220 230 240 YTELFKNHVD YMERTKMLMG STHSQMSTAS ERMDVSRARL NPVARSSGPV SYGFASLENS 250 260 270 280 290 300 VMLDTETICS VGSQSDDSGP PSLQNELDNL SGTLERGAAT DALQQQHHAT SPQSPDSSPV 310 320 330 340 350 360 VPNVPTNVGR STTKKEQRSD NNLYQELSFQ DNEESEENEI VTGSWVHPGE YASSANDNYF 370 380 390 400 410 420 GMGKEVENLI MENNELLATK NALNIVKDDL IVKVDELTGE VEIVREELNA MQQSRTKLRQ 430 440 450 460 470 480 RISELEDELK KAKEQVKQQN TEQEENDVPL AQRKRFTRVE MAMVLMERNQ YKERLMELQE 490 500 510 520 530 540 AVRLTEILRA SRTVDNLDRK SKQSIWKYFS NLFTPSNRPT ERVADGLGGG PMFRHTGGGS 550 560 570 580 590 600 PAHSHGSPSR GSGGGDNRLA LTSGQPPVHP ASAGLANALI MPKDYAEEGS SERISARRRE 610 620 630 640 650 660 QYRQLRAHVQ KEDGRLHAYG WSLPINKASQ EANPNRHSGG VPVPVYCNPL AEASPHMKVF 670 680 690 700 710 720 CAAGVNLHGG FTKNGQSLIP ANSPYAPKST LKIAEITSPT ADQSMEALDR QMARVSLETL 730 740 750 760 770 780 EPETQLSSFV WICTSTHAAS TVSVVDANQS ATVLDAFPIC ASHLLCIASV QGAMESDYAL 790 800 810 820 830 840 LEQSEVVKAG EMLQRPGEGT ELLGKVEFVR VKPKSDDEQN SNEKQQQEEE EAKEATEKSN 850 860 870 880 890 900 EQLPAVSAEE PLGNVEAIKI RQPLPGAPQR LSTDGNQTNN NNNSSSSSNL LFATKSLNPI 910 920 930 940 950 960 LETKDRDEPA MSSVGPTMWL GAQDGWLYVH SSVGRWHECL HRVLLPDAVL AIVHVEARVV 970 980 990 1000 1010 1020 VALANAQLAV FRRQTDGQWD LNSYHLVTLG DRNHSIRCLC VAGERIWAAH RNKIFIVDPV 1030 1040 1050 1060 1070 1080 SLNIVHSLDA HPRKESQVRQ MAATGAGVWV SIRLDSTLRL YNTHTFEHKQ DVDIEPYVSK 1090 1100 1110 1120 1130 1140 MLGTGKLGFS FVRITALMVS CNRLWIGTSN GVIISVPLAE VQPKSSSDPH GQMPLCCMAN 1150 1160 1170 1180 1190 1200 AQLSFHGHRD AVKFFVSVPM LQQPNLNGGL TFTNKRPDML VMCGGEGYID FRINDNDMEN 1210 1220 SIQLEPNQTI ENRGDKSYLI VWHVSQR
	Isoform B. Checksum: 0C3B169946BC0D96	FASTA	1,194	133,212
	10 20 30 40 50 60 MNEQVVSIVQ QLAGSIYQEF ERMINRYDED VVKNLMPLLV NVLECLDASY RINQEQDVEV 70 80 90 100 110 120 ELLREDNEQL VTQYEREKSA RKQSEQKLLE AEDLAEQENK ELATRLESVE SIVRMLELKH 130 140 150 160 170 180 KNSLEHASRL EEREADLKKE YNKLHERYTE LFKNHVDYME RTKMLMGSTH SQMSTASERM 190 200 210 220 230 240 DVSRARLNPV ARSSGPVSYG FASLENSVML DTETICSVGS QSDDSGPPSL QNELDNLSGT 250 260 270 280 290 300 LERGAATDAL QQQHHATSPQ SPDSSPVVPN VPTNVGRSTT KKEQRSDNNL YQELSFQDNE 310 320 330 340 350 360 ESEENEIVTG SWVHPGEYAS SANDNYFGMG KEVENLIMEN NELLATKNAL NIVKDDLIVK 370 380 390 400 410 420 VDELTGEVEI VREELNAMQQ SRTKLRQRIS ELEDELKKAK EQVKQQNTEQ EENDVPLAQR 430 440 450 460 470 480 KRFTRVEMAM VLMERNQYKE RLMELQEAVR LTEILRASRT VDNLDRKSKQ SIWKYFSNLF 490 500 510 520 530 540 TPSNRPTERV ADGLGGGPMF RHTGGGSPAH SHGSPSRGSG GGDNRLALTS GQPPVHPASA 550 560 570 580 590 600 GLANALIMPK DYAEEGSSER ISARRREQYR QLRAHVQKED GRLHAYGWSL PINKASQEAN 610 620 630 640 650 660 PNRHSGGVPV PVYCNPLAEA SPHMKVFCAA GVNLHGGFTK NGQSLIPANS PYAPKSTLKI 670 680 690 700 710 720 AEITSPTADQ SMEALDRQMA RVSLETLEPE TQLSSFVWIC TSTHAASTVS VVDANQSATV 730 740 750 760 770 780 LDAFPICASH LLCIASVQGA MESDYALLEQ SEVVKAGEML QRPGEGTELL GKVEFVRVKP 790 800 810 820 830 840 KSDDEQNSNE KQQQEEEEAK EATEKSNEQL PAVSAEEPLG NVEAIKIRQP LPGAPQRLST 850 860 870 880 890 900 DGNQTNNNNN SSSSSNLLFA TKSLNPILET KDRDEPAMSS VGPTMWLGAQ DGWLYVHSSV 910 920 930 940 950 960 GRWHECLHRV LLPDAVLAIV HVEARVVVAL ANAQLAVFRR QTDGQWDLNS YHLVTLGDRN 970 980 990 1000 1010 1020 HSIRCLCVAG ERIWAAHRNK IFIVDPVSLN IVHSLDAHPR KESQVRQMAA TGAGVWVSIR 1030 1040 1050 1060 1070 1080 LDSTLRLYNT HTFEHKQDVD IEPYVSKMLG TGKLGFSFVR ITALMVSCNR LWIGTSNGVI 1090 1100 1110 1120 1130 1140 ISVPLAEVQP KSSSDPHGQM PLCCMANAQL SFHGHRDAVK FFVSVPMLQQ PNLNGGLTFT 1150 1160 1170 1180 1190 NKRPDMLVMC GGEGYIDFRI NDNDMENSIQ LEPNQTIENR GDKSYLIVWH VSQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Kinesin-dependent axonal transport is mediated by the Sunday Driver (SYD) protein." Bowman A.B., Kamal A., Ritchings B.W., Philp A.V., McGrail M., Gindhart J.G., Goldstein L.S.B. Cell 103:583-594(2000) [PubMed: 11106729] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), INTERACTION WITH KLC.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 937-1227. Strain: Berkeley. Tissue: Embryo and Head.
[5]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
[6]	"RNA editing in Drosophila melanogaster: new targets and functional consequences." Stapleton M., Carlson J.W., Celniker S.E. RNA 12:1922-1932(2006) [PubMed: 17018572] [Abstract] Cited for: RNA EDITING OF POSITION 983.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF262045 mRNA. Translation: AAG36930.1. AE014296 Genomic DNA. Translation: AAF50505.2. AE014296 Genomic DNA. Translation: AAN12032.2. AY060748 mRNA. Translation: AAL28296.1. Different initiation. BT001718 mRNA. Translation: AAN71473.1. Sequence problems. BT016010 mRNA. Translation: AAV36895.1. Sequence problems.
RefSeq	NP_524652.1. NP_729311.2.
UniGene	Dm.6648
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9GQF1. 1 interaction.
Proteomic databases
PRIDE	Q9GQF1.
Genome annotation databases
Ensembl	FBgn0024187. Drosophila melanogaster. [Contig view]
GeneID	43905.
KEGG	dme:Dmel_CG8110.
Organism-specific databases
FlyBase	FBgn0024187. syd.
Phylogenomic databases
HOGENOM	Q9GQF1.
OMA	Q9GQF1. VKTLMPL.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-015659-MON.
Gene expression databases
ArrayExpress	Q9GQF1.
GermOnline	CG8110. Drosophila melanogaster.
Family and domain databases
InterPro	IPR019143. JNK/Rab-associated_protein-1. [Graphical view]
Pfam	PF09744. Jnk-SapK_ap_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	836329.

Entry information

Entry name

JIP3_DROME

Accession

Primary (citable) accession number: Q9GQF1
Secondary accession number(s): Q5U182 , Q8IGL6, Q95SK0, Q9VSC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project