Q9GPS6 (PIMT_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein-L-isoaspartate O-methyltransferase
Short name=PIMT
EC=2.1.1.77

Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl methyltransferase
Protein-beta-aspartate methyltransferase

Gene names

Name:	pcmA
ORF Names:	DDB_G0280979

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity.
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Probable protein-L-isoaspartate O-methyltransferase		PRO_0000326194
Sites
	Active site	106	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9GPS6 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F0549690C150C5E9	FASTA	316	35,529
	10 20 30 40 50 60 MVLILDNDLK PLYFTLAIIS ASFLVKRAYQ STNIYDFFNI KKKCNPFPQS QSELVDLLHY 70 80 90 100 110 120 QKRMVLNKTI VETLKFVDRK LFLENKNVEN PYYDEPKPIG YNATISAPHM HALMLDLLAD 130 140 150 160 170 180 RIPMSNGVAL DIGSGSGYVT ACLGHLMGCT GRVIGVEHIP ELIERSIESI KRLDSTLLDR 190 200 210 220 230 240 IQFLVGDGIK GWKQLKYDII YLGAAIESLQ VARELIDQLK NGGRIVMPVG KSNDFHELMV 250 260 270 280 290 300 VDKNEDGIVS IKSLGVVRFV PLTSKENQLN PKNKPNATKV TNINGEKTLI RCEIIPAPDS 310 NSNNNIKEFE NLINKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Dictyostelium discoideum family of Rho-related proteins." Rivero F., Dislich H., Gloeckner G., Noegel A.A. Nucleic Acids Res. 29:1068-1079(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF310890 Genomic DNA. Translation: AAG45123.1. AAFI02000040 Genomic DNA. Translation: EAL66786.1.
RefSeq	XP_640936.1. XM_635844.1.
3D structure databases
HSSP	HSSP built from PDB template 1I1N based on UniProtKB P22061.
ProteinModelPortal	Q9GPS6.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0214947.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0214947; DDB0214947; DDB_G0280979.
GeneID	8623001.
KEGG	ddi:DDB_G0280979.
Organism-specific databases
dictyBase	DDB_G0280979. pcmA.
Phylogenomic databases
eggNOG	COG2518.
KO	K00573.
OMA	YDEPKPI.
Family and domain databases
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PTHR11579. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT_DICDI

Accession

Primary (citable) accession number: Q9GPS6
Secondary accession number(s): Q54U40

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)