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Q9GPM4 (PGK_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase

EC=2.7.2.3
Gene names
Name:pgkA
ORF Names:DDB_G0287595
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. UniProtKB P00558

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. UniProtKB P00558

Subunit structure

Monomer. Interacts with calmodulin in the presence of Ca2+. Ref.1 UniProtKB P00558

Subcellular location

Cytoplasm By similarity UniProtKB P00558.

Developmental stage

Expressed during development. Expression levels decrease steadily from the initiation of development until culmination. Levels increase after 18 hours of development and peak at 22 hours, after which they decrease again. Ref.3 Ref.4

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 420420Phosphoglycerate kinase
PRO_0000293630

Regions

Nucleotide binding376 – 3794ATP By similarity UniProtKB P00558
Region27 – 293Substrate binding By similarity UniProtKB P00558
Region67 – 704Substrate binding By similarity UniProtKB P00558
Region209 – 22820Calmodulin binding Ref.1

Sites

Binding site431Substrate By similarity UniProtKB P00558
Binding site1261Substrate By similarity UniProtKB P00558
Binding site1741Substrate By similarity UniProtKB P00558
Binding site2231ATP By similarity UniProtKB P00558
Binding site3161ATP; via carbonyl oxygen By similarity UniProtKB P00558
Binding site3471ATP By similarity UniProtKB P00558

Amino acid modifications

Modified residue1991Phosphotyrosine By similarity UniProtKB P00558
Modified residue2061Phosphoserine By similarity UniProtKB P00558 UniProtKB P09411
Modified residue3931Phosphoserine By similarity UniProtKB P09411

Sequences

Sequence LengthMass (Da)Tools
Q9GPM4 [UniParc].

Last modified October 1, 2002. Version 2.
Checksum: 6FEA000CF9B5F83E

FASTA42045,709
        10         20         30         40         50         60 
MSSNKIGNKL SLDKVDVKGK RVLIRVDYNV PLDKNNNITS TQRIDASIPT LEYCLKNGAK 

        70         80         90        100        110        120 
SIVLMSHLGR PDGLVKPEYS LKPVVKVLED QLKRPIKFLS DCVGEQVEKE CANPEEGTVI 

       130        140        150        160        170        180 
LLENLRFHIE EEGSGVDAEG KKVKANPEKV KEFRESLTKL GDVYVNDAFG TAHRAHSSMV 

       190        200        210        220        230        240 
GINLPQKAAG FLMKKELEYF AKALESPSKP FLAILGGAKV SDKIKLIENL LYKVDEMIIG 

       250        260        270        280        290        300 
GGMAFTFKKF IDNKEIGSSL FEKTAEQITK DIIAKAAKNN VKLHFPVDYV IADKFDNDAN 

       310        320        330        340        350        360 
IKTVTQDQGI PEGWMGLDCG PETIKENRDT ISRAKTIVWN GPMGVFEKSN FEAGTKAAMD 

       370        380        390        400        410        420 
DVVNATTNGA ITIIGGGDTA TCAAKYNTED KVSHVSTGGG ASLELLEGKE LPGVTALSDL 

« Hide

References

« Hide 'large scale' references
[1]"Calmodulin binds to and inhibits the activity of phosphoglycerate kinase."
Myre M.A., O'Day D.H.
Biochim. Biophys. Acta 1693:177-183(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CALMODULIN.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"A transcriptional profile of multicellular development in Dictyostelium discoideum."
Van Driessche N., Shaw C., Katoh M., Morio T., Sucgang R., Ibarra M., Kuwayama H., Saito T., Urushihara H., Maeda M., Takeuchi I., Ochiai H., Eaton W., Tollett J., Halter J., Kuspa A., Tanaka Y., Shaulsky G.
Development 129:1543-1552(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[4]"Genome-wide expression analyses of gene regulation during early development of Dictyostelium discoideum."
Iranfar N., Fuller D., Loomis W.F.
Eukaryot. Cell 2:664-670(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[5]"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: AX2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF316577 mRNA. Translation: AAG34561.2.
AAFI02000103 Genomic DNA. Translation: EAL63606.1.

3D structure databases

ProteinModelPortalQ9GPM4.
SMRQ9GPM4. Positions 6-420.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0191349.

Proteomic databases

PRIDEQ9GPM4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191349; DDB0191349; DDB_G0287595.
KEGGddi:DDB_G0287595.

Organism-specific databases

dictyBaseDDB_G0287595. pgkA.

Phylogenomic databases

eggNOGCOG0126.
KOK00927.
OMATEYHANT.
PhylomeDBQ9GPM4.
ProtClustDBPTZ00005.

Enzyme and pathway databases

UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ9GPM4.

Entry information

Entry namePGK_DICDI
AccessionPrimary (citable) accession number: Q9GPM4
Secondary accession number(s): Q54K34
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase