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Q9GPM4

- PGK_DICDI

UniProt

Q9GPM4 - PGK_DICDI

Protein

Phosphoglycerate kinase

Gene

pgkA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei43 – 431SubstrateBy similarity
    Binding sitei126 – 1261SubstrateBy similarity
    Binding sitei174 – 1741SubstrateBy similarity
    Binding sitei223 – 2231ATPBy similarity
    Binding sitei316 – 3161ATP; via carbonyl oxygenBy similarity
    Binding sitei347 – 3471ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi376 – 3794ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calmodulin binding Source: dictyBase
    3. phosphoglycerate kinase activity Source: dictyBase

    GO - Biological processi

    1. gluconeogenesis Source: dictyBase
    2. glycolytic process Source: dictyBase

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00185.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoglycerate kinase (EC:2.7.2.3)
    Gene namesi
    Name:pgkAImported
    ORF Names:DDB_G0287595
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0287595. pgkA.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase
    2. phagocytic vesicle Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 420420Phosphoglycerate kinasePRO_0000293630Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei199 – 1991PhosphotyrosineBy similarity
    Modified residuei206 – 2061PhosphoserineBy similarity
    Modified residuei393 – 3931PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ9GPM4.

    Expressioni

    Developmental stagei

    Expressed during development. Expression levels decrease steadily from the initiation of development until culmination. Levels increase after 18 hours of development and peak at 22 hours, after which they decrease again.2 Publications

    Interactioni

    Subunit structurei

    Monomer. Interacts with calmodulin in the presence of Ca2+.By similarity1 Publication

    Protein-protein interaction databases

    STRINGi44689.DDB_0191349.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9GPM4.
    SMRiQ9GPM4. Positions 6-420.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni27 – 293Substrate bindingBy similarity
    Regioni67 – 704Substrate bindingBy similarity
    Regioni209 – 22820Calmodulin binding1 PublicationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the phosphoglycerate kinase family.Sequence Analysis

    Phylogenomic databases

    eggNOGiCOG0126.
    KOiK00927.
    OMAiIPAGWMA.
    PhylomeDBiQ9GPM4.

    Family and domain databases

    Gene3Di3.40.50.1260. 1 hit.
    3.40.50.1270. 1 hit.
    HAMAPiMF_00145. Phosphoglyc_kinase.
    InterProiIPR001576. Phosphoglycerate_kinase.
    IPR015901. Phosphoglycerate_kinase_C.
    IPR015911. Phosphoglycerate_kinase_CS.
    IPR015824. Phosphoglycerate_kinase_N.
    [Graphical view]
    PANTHERiPTHR11406. PTHR11406. 1 hit.
    PfamiPF00162. PGK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000724. Pgk. 1 hit.
    PRINTSiPR00477. PHGLYCKINASE.
    SUPFAMiSSF53748. SSF53748. 1 hit.
    PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9GPM4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSNKIGNKL SLDKVDVKGK RVLIRVDYNV PLDKNNNITS TQRIDASIPT    50
    LEYCLKNGAK SIVLMSHLGR PDGLVKPEYS LKPVVKVLED QLKRPIKFLS 100
    DCVGEQVEKE CANPEEGTVI LLENLRFHIE EEGSGVDAEG KKVKANPEKV 150
    KEFRESLTKL GDVYVNDAFG TAHRAHSSMV GINLPQKAAG FLMKKELEYF 200
    AKALESPSKP FLAILGGAKV SDKIKLIENL LYKVDEMIIG GGMAFTFKKF 250
    IDNKEIGSSL FEKTAEQITK DIIAKAAKNN VKLHFPVDYV IADKFDNDAN 300
    IKTVTQDQGI PEGWMGLDCG PETIKENRDT ISRAKTIVWN GPMGVFEKSN 350
    FEAGTKAAMD DVVNATTNGA ITIIGGGDTA TCAAKYNTED KVSHVSTGGG 400
    ASLELLEGKE LPGVTALSDL 420
    Length:420
    Mass (Da):45,709
    Last modified:October 1, 2002 - v2
    Checksum:i6FEA000CF9B5F83E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF316577 mRNA. Translation: AAG34561.2.
    AAFI02000103 Genomic DNA. Translation: EAL63606.1.
    RefSeqiXP_637130.1. XM_632038.1.

    Genome annotation databases

    EnsemblProtistsiDDB0191349; DDB0191349; DDB_G0287595.
    GeneIDi8626225.
    KEGGiddi:DDB_G0287595.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF316577 mRNA. Translation: AAG34561.2 .
    AAFI02000103 Genomic DNA. Translation: EAL63606.1 .
    RefSeqi XP_637130.1. XM_632038.1.

    3D structure databases

    ProteinModelPortali Q9GPM4.
    SMRi Q9GPM4. Positions 6-420.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0191349.

    Proteomic databases

    PRIDEi Q9GPM4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0191349 ; DDB0191349 ; DDB_G0287595 .
    GeneIDi 8626225.
    KEGGi ddi:DDB_G0287595.

    Organism-specific databases

    dictyBasei DDB_G0287595. pgkA.

    Phylogenomic databases

    eggNOGi COG0126.
    KOi K00927.
    OMAi IPAGWMA.
    PhylomeDBi Q9GPM4.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00185 .

    Miscellaneous databases

    PROi Q9GPM4.

    Family and domain databases

    Gene3Di 3.40.50.1260. 1 hit.
    3.40.50.1270. 1 hit.
    HAMAPi MF_00145. Phosphoglyc_kinase.
    InterProi IPR001576. Phosphoglycerate_kinase.
    IPR015901. Phosphoglycerate_kinase_C.
    IPR015911. Phosphoglycerate_kinase_CS.
    IPR015824. Phosphoglycerate_kinase_N.
    [Graphical view ]
    PANTHERi PTHR11406. PTHR11406. 1 hit.
    Pfami PF00162. PGK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000724. Pgk. 1 hit.
    PRINTSi PR00477. PHGLYCKINASE.
    SUPFAMi SSF53748. SSF53748. 1 hit.
    PROSITEi PS00111. PGLYCERATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Calmodulin binds to and inhibits the activity of phosphoglycerate kinase."
      Myre M.A., O'Day D.H.
      Biochim. Biophys. Acta 1693:177-183(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CALMODULIN.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX41 Publication.
    3. "A transcriptional profile of multicellular development in Dictyostelium discoideum."
      Van Driessche N., Shaw C., Katoh M., Morio T., Sucgang R., Ibarra M., Kuwayama H., Saito T., Urushihara H., Maeda M., Takeuchi I., Ochiai H., Eaton W., Tollett J., Halter J., Kuspa A., Tanaka Y., Shaulsky G.
      Development 129:1543-1552(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    4. "Genome-wide expression analyses of gene regulation during early development of Dictyostelium discoideum."
      Iranfar N., Fuller D., Loomis W.F.
      Eukaryot. Cell 2:664-670(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    5. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
      Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
      Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: AX2.

    Entry informationi

    Entry nameiPGK_DICDI
    AccessioniPrimary (citable) accession number: Q9GPM4
    Secondary accession number(s): Q54K34
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3