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Q9GPM4

- PGK_DICDI

UniProt

Q9GPM4 - PGK_DICDI

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Protein

Phosphoglycerate kinase

Gene

pgkA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431SubstrateBy similarity
Binding sitei126 – 1261SubstrateBy similarity
Binding sitei174 – 1741SubstrateBy similarity
Binding sitei223 – 2231ATPBy similarity
Binding sitei316 – 3161ATP; via carbonyl oxygenBy similarity
Binding sitei347 – 3471ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi376 – 3794ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calmodulin binding Source: dictyBase
  3. phosphoglycerate kinase activity Source: dictyBase

GO - Biological processi

  1. gluconeogenesis Source: dictyBase
  2. glycolytic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgkAImported
ORF Names:DDB_G0287595
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287595. pgkA.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: dictyBase
  2. phagocytic vesicle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420Phosphoglycerate kinasePRO_0000293630Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991PhosphotyrosineBy similarity
Modified residuei206 – 2061PhosphoserineBy similarity
Modified residuei393 – 3931PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9GPM4.

Expressioni

Developmental stagei

Expressed during development. Expression levels decrease steadily from the initiation of development until culmination. Levels increase after 18 hours of development and peak at 22 hours, after which they decrease again.2 Publications

Interactioni

Subunit structurei

Monomer. Interacts with calmodulin in the presence of Ca2+.By similarity1 Publication

Protein-protein interaction databases

STRINGi44689.DDB_0191349.

Structurei

3D structure databases

ProteinModelPortaliQ9GPM4.
SMRiQ9GPM4. Positions 6-420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni27 – 293Substrate bindingBy similarity
Regioni67 – 704Substrate bindingBy similarity
Regioni209 – 22820Calmodulin binding1 PublicationAdd
BLAST

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Sequence Analysis

Phylogenomic databases

eggNOGiCOG0126.
InParanoidiQ9GPM4.
KOiK00927.
OMAiIPAGWMA.
PhylomeDBiQ9GPM4.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9GPM4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSNKIGNKL SLDKVDVKGK RVLIRVDYNV PLDKNNNITS TQRIDASIPT
60 70 80 90 100
LEYCLKNGAK SIVLMSHLGR PDGLVKPEYS LKPVVKVLED QLKRPIKFLS
110 120 130 140 150
DCVGEQVEKE CANPEEGTVI LLENLRFHIE EEGSGVDAEG KKVKANPEKV
160 170 180 190 200
KEFRESLTKL GDVYVNDAFG TAHRAHSSMV GINLPQKAAG FLMKKELEYF
210 220 230 240 250
AKALESPSKP FLAILGGAKV SDKIKLIENL LYKVDEMIIG GGMAFTFKKF
260 270 280 290 300
IDNKEIGSSL FEKTAEQITK DIIAKAAKNN VKLHFPVDYV IADKFDNDAN
310 320 330 340 350
IKTVTQDQGI PEGWMGLDCG PETIKENRDT ISRAKTIVWN GPMGVFEKSN
360 370 380 390 400
FEAGTKAAMD DVVNATTNGA ITIIGGGDTA TCAAKYNTED KVSHVSTGGG
410 420
ASLELLEGKE LPGVTALSDL
Length:420
Mass (Da):45,709
Last modified:October 1, 2002 - v2
Checksum:i6FEA000CF9B5F83E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF316577 mRNA. Translation: AAG34561.2.
AAFI02000103 Genomic DNA. Translation: EAL63606.1.
RefSeqiXP_637130.1. XM_632038.1.

Genome annotation databases

EnsemblProtistsiDDB0191349; DDB0191349; DDB_G0287595.
GeneIDi8626225.
KEGGiddi:DDB_G0287595.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF316577 mRNA. Translation: AAG34561.2 .
AAFI02000103 Genomic DNA. Translation: EAL63606.1 .
RefSeqi XP_637130.1. XM_632038.1.

3D structure databases

ProteinModelPortali Q9GPM4.
SMRi Q9GPM4. Positions 6-420.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0191349.

Proteomic databases

PRIDEi Q9GPM4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191349 ; DDB0191349 ; DDB_G0287595 .
GeneIDi 8626225.
KEGGi ddi:DDB_G0287595.

Organism-specific databases

dictyBasei DDB_G0287595. pgkA.

Phylogenomic databases

eggNOGi COG0126.
InParanoidi Q9GPM4.
KOi K00927.
OMAi IPAGWMA.
PhylomeDBi Q9GPM4.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00185 .

Miscellaneous databases

PROi Q9GPM4.

Family and domain databases

Gene3Di 3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPi MF_00145. Phosphoglyc_kinase.
InterProi IPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view ]
PANTHERi PTHR11406. PTHR11406. 1 hit.
Pfami PF00162. PGK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000724. Pgk. 1 hit.
PRINTSi PR00477. PHGLYCKINASE.
SUPFAMi SSF53748. SSF53748. 1 hit.
PROSITEi PS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Calmodulin binds to and inhibits the activity of phosphoglycerate kinase."
    Myre M.A., O'Day D.H.
    Biochim. Biophys. Acta 1693:177-183(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CALMODULIN.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX41 Publication.
  3. "A transcriptional profile of multicellular development in Dictyostelium discoideum."
    Van Driessche N., Shaw C., Katoh M., Morio T., Sucgang R., Ibarra M., Kuwayama H., Saito T., Urushihara H., Maeda M., Takeuchi I., Ochiai H., Eaton W., Tollett J., Halter J., Kuspa A., Tanaka Y., Shaulsky G.
    Development 129:1543-1552(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  4. "Genome-wide expression analyses of gene regulation during early development of Dictyostelium discoideum."
    Iranfar N., Fuller D., Loomis W.F.
    Eukaryot. Cell 2:664-670(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
    Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
    Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: AX2.

Entry informationi

Entry nameiPGK_DICDI
AccessioniPrimary (citable) accession number: Q9GPM4
Secondary accession number(s): Q54K34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3