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Protein

Probable mannose-6-phosphate isomerase

Gene

PMIH

Organism
Echinococcus multilocularis (Fox tapeworm)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.By similarity

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathway:iGDP-alpha-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable mannose-6-phosphate isomerase (PMIH)
  2. Phosphomannomutase (EmuJ_000927400)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi103 – 1031ZincBy similarity
Metal bindingi105 – 1051ZincBy similarity
Metal bindingi130 – 1301ZincBy similarity
Metal bindingi277 – 2771ZincBy similarity
Active sitei296 – 2961By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00126; UER00423.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:PMIH
OrganismiEchinococcus multilocularis (Fox tapeworm)
Taxonomic identifieri6211 [NCBI]
Taxonomic lineageiEukaryotaMetazoaPlatyhelminthesCestodaEucestodaCyclophyllideaTaeniidaeEchinococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 433433Probable mannose-6-phosphate isomerasePRO_0000194234Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9GP38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00966. PMI_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9GP38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRLKCASQR YDWGKVGVNS IVYKLQVASE NPDISHVDLP YAELWMGTHP
60 70 80 90 100
SGSSCLWDNP HVTLAAHIQE NPASLGKPSL IYFGRRLPFL FKVLSVAKAL
110 120 130 140 150
SIQAHPDKKM AVRLHAEQPD LYKDGSHKPE MAIALTDFEA LLGFRPLNQI
160 170 180 190 200
LAFIQAFPEL AELTTLELPS PEEKRDVQPP SIKQLYSNLM RSSPEKVEST
210 220 230 240 250
IKSLLNRFTT GSKSVCDPPL SILGVDLQEE DVQALVDLFL RLTQAFPGDV
260 270 280 290 300
GCLSIFFLNY IRLKSGEAIF LKANTPHAYL SGDCVECMAN SDNVVRAGLT
310 320 330 340 350
PKFKDVERLL EMLDYTPLTD SLRLGATQPI PTPEGISMKS FIPPVSEFAV
360 370 380 390 400
DVIQFDAESR GFSLPSVPTA SILLFLHGQG TITCPSTGEA CSQETKFGPG
410 420 430
FVYFVPADMI VNLVSKEDRK GSLHAFRAYV NRK
Length:433
Mass (Da):47,858
Last modified:March 1, 2001 - v1
Checksum:i708C1268CECEB65D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ292370 mRNA. Translation: CAC18544.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ292370 mRNA. Translation: CAC18544.1.

3D structure databases

ProteinModelPortaliQ9GP38.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00126; UER00423.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00966. PMI_I_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "mRNA trans-splicing in the human parasitic cestode Echinococcus multilocularis."
    Brehm K., Jensen K., Frosch M.
    J. Biol. Chem. 275:38311-38318(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: H-95.

Entry informationi

Entry nameiMPI_ECHMU
AccessioniPrimary (citable) accession number: Q9GP38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.