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Protein

Peptidoglycan-recognition protein LC

Gene

PGRP-LC

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major activator of the imd/Relish pathway and is likely to encode a pattern recognition molecule for the humoral immune response (PubMed:11872802, PubMed:22022271). Required for Relish processing and nuclear translocation following proteolytic cleavage (PubMed:11872802). Involved in the response to lipopolysaccharide (LPS) and peptidoglycan of Gram-negative bacteria (PubMed:11872802). The different isoforms probably display different recognition capabilities to various microbial patterns (PubMed:12777387, PubMed:16006509).4 Publications
Isoform a: Mediates the response to LPS and Gram-negative bacteria.2 Publications
Isoform x: Mediates the response to LPS, peptidoglycan and Gram-negative bacteria.2 Publications

GO - Molecular functioni

  • lipopolysaccharide receptor activity Source: FlyBase
  • peptidoglycan binding Source: FlyBase
  • peptidoglycan receptor activity Source: FlyBase
  • protein dimerization activity Source: FlyBase
  • signaling pattern recognition receptor activity Source: FlyBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • antibacterial humoral response Source: FlyBase
  • antimicrobial humoral response Source: FlyBase
  • defense response to Gram-negative bacterium Source: FlyBase
  • defense response to virus Source: FlyBase
  • immune response Source: FlyBase
  • innate immune response Source: FlyBase
  • peptidoglycan recognition protein signaling pathway Source: FlyBase
  • phagocytosis Source: FlyBase
  • positive regulation of antibacterial peptide biosynthetic process Source: FlyBase
  • positive regulation of biosynthetic process of antibacterial peptides active against Gram-negative bacteria Source: FlyBase
  • regulation of humoral immune response Source: FlyBase
  • regulation of melanization defense response Source: FlyBase
  • response to bacterium Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan-recognition protein LC
Alternative name(s):
Immune response deficient 7 protein
Gene namesi
Name:PGRP-LC
Synonyms:ird7, PGRPLC
ORF Names:CG4432
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0035976. PGRP-LC.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 291CytoplasmicSequence analysisAdd BLAST291
Transmembranei292 – 312Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini313 – 520ExtracellularSequence analysisAdd BLAST208

GO - Cellular componenti

  • apicolateral plasma membrane Source: FlyBase
  • cytoplasm Source: FlyBase
  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: FlyBase
  • plasma membrane Source: FlyBase
  • spanning component of plasma membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Larvae infected with Gram-negative bacteria fail to induce tracheal expression of the antimicrobial peptide gene Drs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002206241 – 520Peptidoglycan-recognition protein LCAdd BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi389N-linked (GlcNAc...)1 Publication1
Disulfide bondi390 ↔ 3962 Publications
Glycosylationi515N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9GNK5.
PRIDEiQ9GNK5.

Expressioni

Tissue specificityi

Expressed in the fat body and hemocytes.1 Publication

Developmental stagei

Expressed during larval and pupal stages.1 Publication

Gene expression databases

BgeeiFBgn0035976.
ExpressionAtlasiQ9GNK5. baseline.
GenevisibleiQ9GNK5. DM.

Interactioni

GO - Molecular functioni

  • protein dimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi64460. 76 interactors.
IntActiQ9GNK5. 3 interactors.
MINTiMINT-948537.
STRINGi7227.FBpp0088492.

Structurei

Secondary structure

1520
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi358 – 361Combined sources4
Beta strandi375 – 384Combined sources10
Helixi393 – 409Combined sources17
Beta strandi419 – 422Combined sources4
Beta strandi428 – 432Combined sources5
Turni433 – 435Combined sources3
Beta strandi440 – 442Combined sources3
Helixi444 – 447Combined sources4
Beta strandi451 – 457Combined sources7
Beta strandi460 – 462Combined sources3
Helixi466 – 481Combined sources16
Beta strandi484 – 493Combined sources10
Helixi494 – 497Combined sources4
Helixi502 – 504Combined sources3
Helixi507 – 511Combined sources5
Turni512 – 515Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z6IX-ray2.50A355-520[»]
2F2LX-ray2.10A355-520[»]
X418-434[»]
ProteinModelPortaliQ9GNK5.
SMRiQ9GNK5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9GNK5.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IXCZ. Eukaryota.
ENOG410ZJPX. LUCA.
GeneTreeiENSGT00390000016833.
InParanoidiQ9GNK5.
KOiK01446.
OMAiTEMSQCP.
OrthoDBiEOG091G0O6Z.
PhylomeDBiQ9GNK5.

Family and domain databases

CDDicd06583. PGRP. 1 hit.
Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a1 Publication (identifier: Q9GNK5-1) [UniParc]FASTAAdd to basket
Also known as: B1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPFSNETEMS QCSNAKRRVN DPLTGPKNCS TSSTDSGVIL NDNVAAFRPE
60 70 80 90 100
KETKDRGTGE GQFQSKSEEK TESKRISVEH TVNITTENVG KTSSPAVSIR
110 120 130 140 150
STTISVVSID DNAIDSSSID SDSEAEAEDY TVQKLGHQVT YPPNSSHLRD
160 170 180 190 200
LNQGLTVISR HVAPGEAAVP PPNPLEAGIV AKQILNGNLA VATPTSPAGG
210 220 230 240 250
ATQGIGSIAL TNSTDVTFGD KHFYEGPVTI QQFLIDNRDK WKPGEGPAGG
260 270 280 290 300
QDNPAFNGGP STNGSAPGSK HEDPAQTPPI CPFLPNTVGR KAVTVTVVFV
310 320 330 340 350
TLTFLLGIVL ATTTNLFGKT LNQSKIRDDD DYRQNIPINS TIDLDNIGGG
360 370 380 390 400
LILRFVERQQ WLAQPPQKEI PDLELPVGLV IALPTNSENC STQAICVLRV
410 420 430 440 450
RLLQTYDIES SQKCDIAYNF LIGGDGNVYV GRGWNKMGAH MNNINYDSQS
460 470 480 490 500
LSFAYIGSFK TIQPSAKQLS VTRLLLERGV KLGKIAPSYR FTASSKLMPS
510 520
VTDFKADALY ASFANWTHWS
Length:520
Mass (Da):56,046
Last modified:March 1, 2001 - v1
Checksum:i3215F8380857DFC1
GO
Isoform x1 Publication (identifier: Q9GNK5-2) [UniParc]FASTAAdd to basket
Also known as: A1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     324-520: SKIRDDDDYR...ASFANWTHWS → TDLDVIDNST...KKWPHWSHEI

Show »
Length:500
Mass (Da):54,094
Checksum:iA0459DE723A8C720
GO
Isoform y1 Publication (identifier: Q9GNK5-3) [UniParc]FASTAAdd to basket
Also known as: C1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     324-520: SKIRDDDDYR...ASFANWTHWS → TNDPEIHVDG...KTWPDLHMTQ

Show »
Length:511
Mass (Da):55,545
Checksum:i0969EE0AF556F549
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Isoform y1 Publication (identifier: Q9GNK5-3)
Sequence conflicti338I → V in AAS65052 (PubMed:10731132).Curated1
Sequence conflicti355L → F in AAS65052 (PubMed:10731132).Curated1
Sequence conflicti373T → K in AAQ16306 (PubMed:12777387).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050759324 – 520SKIRD…WTHWS → TDLDVIDNSTLVILKVAEWG GRPAKRMLDAQQLPINRVVI SHTAAEGCESREVCSARVNV VQSFHMDSWGWDHIGYNFLV GGDGRVYEGRGWDYVGAHTK GYNRGSIGISFIGTFTTRKP NERQLEACQLLLQEGVRLKK LTTNYRLYGHRQLSATESPG EELYKIIKKWPHWSHEI in isoform x. 4 PublicationsAdd BLAST197
Alternative sequenceiVSP_050760324 – 520SKIRD…WTHWS → TNDPEIHVDGKLVVISIKGW GGMPTRGNLKPLKLPVSKVI ISETPPEICTTQDSCSYWTR VTQSRHMDTFNWSQVGYNFL VGGDGRIYEGRGWNYMGDHT RDNNNNSIGITFLGTFRRQE PTPKSLEACQLLIAQGVRLK KLKPDYQLLGHRQITGTLMP GEELYRIIQTWNNWYNLTKT WPDLHMTQ in isoform y. 2 PublicationsAdd BLAST197

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF207539 mRNA. Translation: AAG23733.1.
AF500096 mRNA. Translation: AAM18530.1.
AY327466 mRNA. Translation: AAQ16306.1.
AE014296 Genomic DNA. Translation: AAF50302.3.
AE014296 Genomic DNA. Translation: AAN11957.1.
AE014296 Genomic DNA. Translation: AAS65052.1.
AY119048 mRNA. Translation: AAM50908.1.
BT025958 mRNA. Translation: ABG02202.1.
RefSeqiNP_001163396.1. NM_001169925.1. [Q9GNK5-2]
NP_001246693.1. NM_001259764.1. [Q9GNK5-1]
NP_648298.1. NM_140041.4. [Q9GNK5-1]
NP_729468.2. NM_168324.4. [Q9GNK5-2]
NP_996030.1. NM_206308.4.
UniGeneiDm.1437.

Genome annotation databases

EnsemblMetazoaiFBtr0089491; FBpp0088492; FBgn0035976. [Q9GNK5-1]
FBtr0308349; FBpp0300668; FBgn0035976. [Q9GNK5-1]
GeneIDi39063.
KEGGidme:Dmel_CG4432.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF207539 mRNA. Translation: AAG23733.1.
AF500096 mRNA. Translation: AAM18530.1.
AY327466 mRNA. Translation: AAQ16306.1.
AE014296 Genomic DNA. Translation: AAF50302.3.
AE014296 Genomic DNA. Translation: AAN11957.1.
AE014296 Genomic DNA. Translation: AAS65052.1.
AY119048 mRNA. Translation: AAM50908.1.
BT025958 mRNA. Translation: ABG02202.1.
RefSeqiNP_001163396.1. NM_001169925.1. [Q9GNK5-2]
NP_001246693.1. NM_001259764.1. [Q9GNK5-1]
NP_648298.1. NM_140041.4. [Q9GNK5-1]
NP_729468.2. NM_168324.4. [Q9GNK5-2]
NP_996030.1. NM_206308.4.
UniGeneiDm.1437.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z6IX-ray2.50A355-520[»]
2F2LX-ray2.10A355-520[»]
X418-434[»]
ProteinModelPortaliQ9GNK5.
SMRiQ9GNK5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64460. 76 interactors.
IntActiQ9GNK5. 3 interactors.
MINTiMINT-948537.
STRINGi7227.FBpp0088492.

Proteomic databases

PaxDbiQ9GNK5.
PRIDEiQ9GNK5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089491; FBpp0088492; FBgn0035976. [Q9GNK5-1]
FBtr0308349; FBpp0300668; FBgn0035976. [Q9GNK5-1]
GeneIDi39063.
KEGGidme:Dmel_CG4432.

Organism-specific databases

CTDi39063.
FlyBaseiFBgn0035976. PGRP-LC.

Phylogenomic databases

eggNOGiENOG410IXCZ. Eukaryota.
ENOG410ZJPX. LUCA.
GeneTreeiENSGT00390000016833.
InParanoidiQ9GNK5.
KOiK01446.
OMAiTEMSQCP.
OrthoDBiEOG091G0O6Z.
PhylomeDBiQ9GNK5.

Miscellaneous databases

EvolutionaryTraceiQ9GNK5.
GenomeRNAii39063.
PROiQ9GNK5.

Gene expression databases

BgeeiFBgn0035976.
ExpressionAtlasiQ9GNK5. baseline.
GenevisibleiQ9GNK5. DM.

Family and domain databases

CDDicd06583. PGRP. 1 hit.
Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPGPLC_DROME
AccessioniPrimary (citable) accession number: Q9GNK5
Secondary accession number(s): Q1EBX6
, Q7YW58, Q8T5Q2, Q9VSV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.