Reviewed, UniProtKB/Swiss-Prot Q9GMY6 (PEPA_CANFA)

Names and origin

Protein names

Recommended name:
    Pepsin A
    EC=3.4.23.1

Gene names

Name:	PGA
Synonyms:	PGNA

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent By similarity.
Catalytic activity	Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Potential
	Propeptide	16 – 60	45	Activation peptide By similarity		PRO_0000026011
	Chain	61 – 386	326	Pepsin A		PRO_0000026012
Sites
	Active site	92	1	By similarity
	Active site	275	1	By similarity
Amino acid modifications
	Disulfide bond	105 ↔ 110		By similarity
	Disulfide bond	266 ↔ 270		By similarity
	Disulfide bond	309 ↔ 342		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9GMY6-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 5F0D1598322390C5	FASTA	386	41,551
	10 20 30 40 50 60 MKWLLLISLV ALSECAIVKV PLVRKKSLRQ NLIEHGLLND FLKNQSPNPA SKYFPQEPTV 70 80 90 100 110 120 LATQSLKNYM DMEYFGTIGI GTPPQEFTVI FDTGSSNLWV PSVYCSSPAC SNHNRFNPQE 130 140 150 160 170 180 SSTYQGTNRP VSIAYGTGSM TGILGYDTVQ VGGIADTNQI FGLSETEPGS FLYYAPFDGI 190 200 210 220 230 240 LGLAYPQISA SGATPVFDNM WNEGLVSQDL FSVYLSSDDQ SGSVVMFGGI DSSYYSGNLN 250 260 270 280 290 300 WVPVSVEGYW QITVDSVTMN GQAIACSDGC QAIVDTGTSL LAGPTNAIAN IQSYIGASQN 310 320 330 340 350 360 SYGQMVISCS AINSLPDIVF TINGIQYPLP PSAYILQSQQ GCVSGFQGMN LPTASGELWI 370 380 LGDVFIRQYF AVFDRANNQV GLAPVA

References

[1]	"Phylogenetic position of Eulipotyphla inferred from the cDNA sequences of pepsinogens A and C." Narita Y., Oda S., Takenaka O., Kageyama T. Mol. Phylogenet. Evol. 21:32-42(2001) [PubMed: 11603935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB047246 mRNA. Translation: BAB11752.1.
RefSeq	NP_001003117.1.
UniGene	Cfa.27400
3D structure databases
HSSP	HSSP built from PDB template 1PSN based on UniProtKB P00790.
SMR	Q9GMY6. Positions 17-386.
ModBase	Search...
Protein family/group databases
MEROPS	A01.001.
Genome annotation databases
Ensembl	ENSCAFT00000025862; ENSCAFP00000024018; ENSCAFG00000016308; Canis familiaris. [Genome view]
GeneID	403711.
KEGG	cfa:403711.
Organism-specific databases
CTD	403711.
Phylogenomic databases
HOVERGEN	Q9GMY6.
Enzyme and pathway databases
BRENDA	3.4.23.1. 463.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEPA_CANFA

Accession

Primary (citable) accession number: Q9GMY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	March 1, 2001
Last modified:	September 1, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)