Q9GMF1 (DGAT1_CHLAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol O-acyltransferase 1
EC=2.3.1.20

Alternative name(s):
Diglyceride acyltransferase

Gene names

Name:	DGAT1
Synonyms:	DGAT

Organism

Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops)

Taxonomic identifier

9534 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Chlorocebus

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly By similarity.
Catalytic activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Pathway	Lipid metabolism; glycerolipid metabolism.
Subunit structure	Homodimer or homotetramer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	diacylglycerol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Diacylglycerol O-acyltransferase 1		PRO_0000207653
Regions
	Topological domain	1 – 90	90	Cytoplasmic Potential
	Transmembrane	91 – 112	22	Helical; Potential
	Topological domain	113 – 223	111	Lumenal Potential
	Transmembrane	224 – 232	9	Helical; Potential
	Topological domain	233	1	Cytoplasmic Potential
	Transmembrane	234 – 242	9	Helical; Potential
	Topological domain	243 – 491	249	Lumenal Potential
	Region	1 – 94	94	Involved in homomerization By similarity
Sites
	Active site	418	1	By similarity
Amino acid modifications
	Modified residue	20	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9GMF1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: BFD3683453D588DB	FASTA	491	55,644
	10 20 30 40 50 60 MGDRGGAGGT RRRRTGSRPS SHGGGGPAAA EEEVRDAAAG PDMGAAGDAP APAPSKDADD 70 80 90 100 110 120 GVASGHWELR CHRLQDSLFS SDSGFNNYRG ILNWCVVMLI LSNARLFLEN LIKYGILVDP 130 140 150 160 170 180 IQVVSLFLKD PYSWPAPCLV IAANVFAVAA FQVEKRLAVG ALTEQAGLLL HVANLATILC 190 200 210 220 230 240 FPAAVVLLVE SITPVGSLLA LMVHTILFLK LFSYRDVNLW CRRARAKAAS AGKRASSAAA 250 260 270 280 290 300 PHTVSYPDNL TYRDLYYFLF APTLCYELNF PRSPRIRKRF LLRRILEMLF FTQLQVGLIQ 310 320 330 340 350 360 QWMVPTIQNS MKPFKDMDYS RIIERLLKLA VPNHLIWLIF FYWLFHSCLN AVAELMQFGD 370 380 390 400 410 420 REFYRDWWNS ESVTYFWQNW NIPVHKWCIR HFYKPMLRRG SSRWMARIGV FLASAFFHEY 430 440 450 460 470 480 LVSVPLRMFR LWAFTGMMAQ IPLAWFVGRF FQGNYGNAAV WLTLIIGQPI AVLMYVHDYY 490 VLNYEAPVAG A

References

[1]	Joyce C.W., Davis M.A., Anderson R.A., Rudel L.L. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF236018 mRNA. Translation: AAF98557.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG051341.
Family and domain databases
InterPro	IPR004299. MBOAT_fam. IPR014371. Oat_ACAT_DAG_ARE. [Graphical view]
PANTHER	PTHR10408. PTHR10408. 1 hit.
Pfam	PF03062. MBOAT. 1 hit. [Graphical view]
PIRSF	PIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DGAT1_CHLAE

Accession

Primary (citable) accession number: Q9GMF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	March 1, 2001
Last modified:	October 19, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program