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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

Gene

RPN1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarity1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
Ribophorin I
Short name:
RPN-I
Ribophorin-1
Gene namesi
Name:RPN1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 435LumenalSequence analysisAdd BLAST411
Transmembranei436 – 456HelicalSequence analysisAdd BLAST21
Topological domaini457 – 607CytoplasmicBy similarityAdd BLAST151

GO - Cellular componenti

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24By similarityAdd BLAST24
ChainiPRO_000033125425 – 608Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1Add BLAST584

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188N6-acetyllysineBy similarity1
Glycosylationi300N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei539N6-acetyllysine; alternateBy similarity1
Cross-linki539Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9GMB0.
PeptideAtlasiQ9GMB0.
PRIDEiQ9GMB0.

Expressioni

Tissue specificityi

Detected in liver (at protein level).1 Publication

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).By similarity1 Publication

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000012376.

Structurei

3D structure databases

SMRiQ9GMB0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the OST1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2291. Eukaryota.
ENOG410XQVZ. LUCA.
HOGENOMiHOG000247000.
HOVERGENiHBG012864.
InParanoidiQ9GMB0.
KOiK12666.

Family and domain databases

InterProiView protein in InterPro
IPR007676. Ribophorin_I.
PANTHERiPTHR21049. PTHR21049. 1 hit.
PfamiView protein in Pfam
PF04597. Ribophorin_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9GMB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAPAVCLLP LLLLLWAWAP APGRASPEAL PLVNEDVKRT VDLSSHLAKV
60 70 80 90 100
TAEVVLAHAG SSSSPRAASF LLALEPELEA RLAHLGVQVK GEDEEENNLE
110 120 130 140 150
VRETKIKGKR GRFFTVTLPV ALDPGAKISV TVETVYTHVL QPYPTQITQS
160 170 180 190 200
EKQFVVFEGN HYFYSPYPTK SQSMRVKLAS RNVESYTKLG NPTRSEDLLD
210 220 230 240 250
YGPFRDVPPY SQDTFKVHSE NNSPFLTITS MTRVIEVSHW GNIAVEENVD
260 270 280 290 300
LKHTGAVLKG PFSRYDYQRQ PDSGISSIRS FKTILPAAAQ DVYYRDEIGN
310 320 330 340 350
VSTSHLLILD DSVEMEIRPR FPLFGGWKTH YIVGYNLPSY EYLYNLGDQY
360 370 380 390 400
ALKMRLVDHV FDEQVIDSLT VKIILPEGAK NIQVDSPYEI SRAPDELHYT
410 420 430 440 450
YLDTFGRPVI VAHKKNLVEQ HIQDIVVHYT FNKVLMLQEP LLVVAAFYIL
460 470 480 490 500
FFTVIVYVRL DFSITKDPAA EARMKVACIT EQVLTLVNKR IGLYRHFDET
510 520 530 540 550
INRYKQSRDV STLNSGKKSL ELEHKALTSE VALLQSRLKT EGSDLCDKVS
560 570 580 590 600
EMQKLDAQVK ELVLKSAVEA ERLVAGKLKK DTYIENEKLI SGKRQELVTK

IDHILDAL
Length:608
Mass (Da):68,695
Last modified:March 1, 2001 - v1
Checksum:iD8928F5AF03F851C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ293582 mRNA. Translation: CAC04096.1.
RefSeqiNP_999498.1. NM_214333.1.
UniGeneiSsc.12528.

Genome annotation databases

GeneIDi397606.
KEGGissc:397606.

Similar proteinsi

Entry informationi

Entry nameiRPN1_PIG
AccessioniPrimary (citable) accession number: Q9GMB0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2001
Last modified: May 10, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families