Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9GM50

- UCHL1_HORSE

UniProt

Q9GM50 - UCHL1_HORSE

Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Gene

UCHL1

Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (05 Jul 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei1 – 11Susceptible to oxidationBy similarity
    Sitei6 – 61Susceptible to oxidationBy similarity
    Sitei12 – 121Susceptible to oxidationBy similarity
    Active sitei90 – 901NucleophilePROSITE-ProRule annotation
    Active sitei161 – 1611Proton donorPROSITE-ProRule annotation
    Sitei176 – 1761Important for enzyme activityBy similarity
    Sitei179 – 1791Susceptible to oxidationBy similarity
    Sitei220 – 2201Susceptible to oxidationBy similarity

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. ubiquitin-specific protease activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Ligase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC12.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12, EC:6.-.-.-)
    Short name:
    UCH-L1
    Alternative name(s):
    Neuron cytoplasmic protein 9.5
    PGP 9.5
    Short name:
    PGP9.5
    Ubiquitin thioesterase L1
    Gene namesi
    Name:UCHL1
    OrganismiEquus caballus (Horse)
    Taxonomic identifieri9796 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
    ProteomesiUP000002281: Unplaced

    Subcellular locationi

    Cytoplasm By similarity. Endoplasmic reticulum membrane By similarity; Lipid-anchor By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 220220Ubiquitin carboxyl-terminal hydrolase isozyme L1PRO_0000211054Add
    BLAST
    Propeptidei221 – 2233Removed in mature formBy similarityPRO_0000414310

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi220 – 2201S-farnesyl cysteineBy similarity

    Post-translational modificationi

    O-glycosylated.By similarity

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Oxidation, Prenylation

    Interactioni

    Subunit structurei

    Monomer. Homodimer. Interacts with COPS5 and SNCA By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9GM50.
    SMRiQ9GM50. Positions 1-223.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni5 – 106Interaction with ubiquitinBy similarity
    Regioni151 – 1566Interaction with ubiquitinBy similarity
    Regioni211 – 2166Interaction with ubiquitinBy similarity

    Sequence similaritiesi

    Belongs to the peptidase C12 family.Curated

    Phylogenomic databases

    eggNOGiNOG327708.
    HOGENOMiHOG000182400.
    HOVERGENiHBG075483.
    InParanoidiQ9GM50.
    KOiK05611.

    Family and domain databases

    Gene3Di3.40.532.10. 1 hit.
    InterProiIPR001578. Peptidase_C12_UCH.
    [Graphical view]
    PANTHERiPTHR10589. PTHR10589. 1 hit.
    PfamiPF01088. Peptidase_C12. 1 hit.
    [Graphical view]
    PRINTSiPR00707. UBCTHYDRLASE.
    PROSITEiPS00140. UCH_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9GM50-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQLKPMEINP EMLNKVLARL GVAGQWRFVD VLGLEEETLG SVPAPACALL    50
    LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA 100
    NNQDKLEFED GSVLKQFLSE TEKLSPEDRA KCFEKNEAIQ AAHDAVAQEG 150
    QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DLLLQDAAKV 200
    CREFTEREQG EVRFSAVALC KAA 223
    Length:223
    Mass (Da):24,888
    Last modified:July 5, 2005 - v2
    Checksum:iA32AFE7D1721CAD6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti187 – 1871A → T in BAB13757. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB049188 mRNA. Translation: BAB13757.1.
    AB013344 mRNA. Translation: BAA28214.1.
    RefSeqiNP_001075289.1. NM_001081820.1.
    UniGeneiEca.11520.

    Genome annotation databases

    GeneIDi100033838.
    KEGGiecb:100033838.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB049188 mRNA. Translation: BAB13757.1 .
    AB013344 mRNA. Translation: BAA28214.1 .
    RefSeqi NP_001075289.1. NM_001081820.1.
    UniGenei Eca.11520.

    3D structure databases

    ProteinModelPortali Q9GM50.
    SMRi Q9GM50. Positions 1-223.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C12.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100033838.
    KEGGi ecb:100033838.

    Organism-specific databases

    CTDi 7345.

    Phylogenomic databases

    eggNOGi NOG327708.
    HOGENOMi HOG000182400.
    HOVERGENi HBG075483.
    InParanoidi Q9GM50.
    KOi K05611.

    Family and domain databases

    Gene3Di 3.40.532.10. 1 hit.
    InterProi IPR001578. Peptidase_C12_UCH.
    [Graphical view ]
    PANTHERi PTHR10589. PTHR10589. 1 hit.
    Pfami PF01088. Peptidase_C12. 1 hit.
    [Graphical view ]
    PRINTSi PR00707. UBCTHYDRLASE.
    PROSITEi PS00140. UCH_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning of equine ubiquitin C-terminal hydrolase (PGP9.5)."
      Sato F., Harigai N., Iwanaga T., Hasegawa T., Ishida N.
      Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Yanase H., Kitamura H., Iwanaga T., Kanehira K., Okita K.
      Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 50-188.
      Tissue: Brain.

    Entry informationi

    Entry nameiUCHL1_HORSE
    AccessioniPrimary (citable) accession number: Q9GM50
    Secondary accession number(s): O62662
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3