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Q9GM50 (UCHL1_HORSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Short name=UCH-L1
EC=3.4.19.12
EC=6.-.-.-
Alternative name(s):
Neuron cytoplasmic protein 9.5
PGP 9.5
Short name=PGP9.5
Ubiquitin thioesterase L1
Gene names
Name:UCHL1
OrganismEquus caballus (Horse) [Reference proteome]
Taxonomic identifier9796 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus ›

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Monomer. Homodimer. Interacts with COPS5 and SNCA By similarity.

Subcellular location

Cytoplasm By similarity. Endoplasmic reticulum membrane; Lipid-anchor By similarity.

Post-translational modification

O-glycosylated By similarity.

Miscellaneous

In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8 By similarity.

Sequence similarities

Belongs to the peptidase C12 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 220220Ubiquitin carboxyl-terminal hydrolase isozyme L1
PRO_0000211054
Propeptide221 – 2233Removed in mature form By similarity
PRO_0000414310

Regions

Region5 – 106Interaction with ubiquitin By similarity
Region151 – 1566Interaction with ubiquitin By similarity
Region211 – 2166Interaction with ubiquitin By similarity

Sites

Active site901Nucleophile By similarity
Active site1611Proton donor By similarity
Site11Susceptible to oxidation By similarity
Site61Susceptible to oxidation By similarity
Site121Susceptible to oxidation By similarity
Site1761Important for enzyme activity By similarity
Site1791Susceptible to oxidation By similarity
Site2201Susceptible to oxidation By similarity

Amino acid modifications

Lipidation2201S-farnesyl cysteine By similarity

Experimental info

Sequence conflict1871A → T in BAB13757. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9GM50 [UniParc].

Last modified July 5, 2005. Version 2.
Checksum: A32AFE7D1721CAD6

FASTA22324,888
        10         20         30         40         50         60 
MQLKPMEINP EMLNKVLARL GVAGQWRFVD VLGLEEETLG SVPAPACALL LLFPLTAQHE 

        70         80         90        100        110        120 
NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLEFED GSVLKQFLSE 

       130        140        150        160        170        180 
TEKLSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP 

       190        200        210        220 
FPVNHGASSE DLLLQDAAKV CREFTEREQG EVRFSAVALC KAA

« Hide

References

[1]"cDNA cloning of equine ubiquitin C-terminal hydrolase (PGP9.5)."
Sato F., Harigai N., Iwanaga T., Hasegawa T., Ishida N.
Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Yanase H., Kitamura H., Iwanaga T., Kanehira K., Okita K.
Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 50-188.
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB049188 mRNA. Translation: BAB13757.1.
AB013344 mRNA. Translation: BAA28214.1.
RefSeqNP_001075289.1. NM_001081820.1.
UniGeneEca.11520.

3D structure databases

ProteinModelPortalQ9GM50.
SMRQ9GM50. Positions 1-223.
ModBaseSearch...

Protein family/group databases

MEROPSC12.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100033838.
KEGGecb:100033838.

Organism-specific databases

CTD7345.

Phylogenomic databases

eggNOGNOG327708.
HOGENOMHOG000182400.
HOVERGENHBG075483.
InParanoidQ9GM50.
KOK05611.
OrthoDBEOG4RJG2F.

Family and domain databases

Gene3D3.40.532.10. 1 hit.
InterProIPR001578. Peptidase_C12.
[Graphical view]
PANTHERPTHR10589. PTHR10589. 1 hit.
PfamPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSPR00707. UBCTHYDRLASE.
PROSITEPS00140. UCH_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUCHL1_HORSE
AccessionPrimary (citable) accession number: Q9GM50
Secondary accession number(s): O62662
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 5, 2005
Last modified: April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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