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Q9GM50

- UCHL1_HORSE

UniProt

Q9GM50 - UCHL1_HORSE

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Protein
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Gene
UCHL1
Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei1 – 11Susceptible to oxidation By similarity
Sitei6 – 61Susceptible to oxidation By similarity
Sitei12 – 121Susceptible to oxidation By similarity
Active sitei90 – 901Nucleophile By similarity
Active sitei161 – 1611Proton donor By similarity
Sitei176 – 1761Important for enzyme activity By similarity
Sitei179 – 1791Susceptible to oxidation By similarity
Sitei220 – 2201Susceptible to oxidation By similarity

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. ubiquitin-specific protease activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC12.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12, EC:6.-.-.-)
Short name:
UCH-L1
Alternative name(s):
Neuron cytoplasmic protein 9.5
PGP 9.5
Short name:
PGP9.5
Ubiquitin thioesterase L1
Gene namesi
Name:UCHL1
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
ProteomesiUP000002281: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Ubiquitin carboxyl-terminal hydrolase isozyme L1
PRO_0000211054Add
BLAST
Propeptidei221 – 2233Removed in mature form By similarity
PRO_0000414310

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi220 – 2201S-farnesyl cysteine By similarity

Post-translational modificationi

O-glycosylated By similarity.

Keywords - PTMi

Glycoprotein, Lipoprotein, Oxidation, Prenylation

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with COPS5 and SNCA By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9GM50.
SMRiQ9GM50. Positions 1-223.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni5 – 106Interaction with ubiquitin By similarity
Regioni151 – 1566Interaction with ubiquitin By similarity
Regioni211 – 2166Interaction with ubiquitin By similarity

Sequence similaritiesi

Belongs to the peptidase C12 family.

Phylogenomic databases

eggNOGiNOG327708.
HOGENOMiHOG000182400.
HOVERGENiHBG075483.
InParanoidiQ9GM50.
KOiK05611.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
PROSITEiPS00140. UCH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9GM50-1 [UniParc]FASTAAdd to Basket

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MQLKPMEINP EMLNKVLARL GVAGQWRFVD VLGLEEETLG SVPAPACALL    50
LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA 100
NNQDKLEFED GSVLKQFLSE TEKLSPEDRA KCFEKNEAIQ AAHDAVAQEG 150
QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DLLLQDAAKV 200
CREFTEREQG EVRFSAVALC KAA 223
Length:223
Mass (Da):24,888
Last modified:July 5, 2005 - v2
Checksum:iA32AFE7D1721CAD6
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti187 – 1871A → T in BAB13757. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB049188 mRNA. Translation: BAB13757.1.
AB013344 mRNA. Translation: BAA28214.1.
RefSeqiNP_001075289.1. NM_001081820.1.
UniGeneiEca.11520.

Genome annotation databases

GeneIDi100033838.
KEGGiecb:100033838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB049188 mRNA. Translation: BAB13757.1 .
AB013344 mRNA. Translation: BAA28214.1 .
RefSeqi NP_001075289.1. NM_001081820.1.
UniGenei Eca.11520.

3D structure databases

ProteinModelPortali Q9GM50.
SMRi Q9GM50. Positions 1-223.
ModBasei Search...

Protein family/group databases

MEROPSi C12.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100033838.
KEGGi ecb:100033838.

Organism-specific databases

CTDi 7345.

Phylogenomic databases

eggNOGi NOG327708.
HOGENOMi HOG000182400.
HOVERGENi HBG075483.
InParanoidi Q9GM50.
KOi K05611.

Family and domain databases

Gene3Di 3.40.532.10. 1 hit.
InterProi IPR001578. Peptidase_C12_UCH.
[Graphical view ]
PANTHERi PTHR10589. PTHR10589. 1 hit.
Pfami PF01088. Peptidase_C12. 1 hit.
[Graphical view ]
PRINTSi PR00707. UBCTHYDRLASE.
PROSITEi PS00140. UCH_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning of equine ubiquitin C-terminal hydrolase (PGP9.5)."
    Sato F., Harigai N., Iwanaga T., Hasegawa T., Ishida N.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Yanase H., Kitamura H., Iwanaga T., Kanehira K., Okita K.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 50-188.
    Tissue: Brain.

Entry informationi

Entry nameiUCHL1_HORSE
AccessioniPrimary (citable) accession number: Q9GM50
Secondary accession number(s): O62662
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 5, 2005
Last modified: April 16, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8 By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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