Reviewed, UniProtKB/Swiss-Prot Q9GM01 (GALT9_MACFA)

Names and origin

Protein names

Recommended name:
    Polypeptide N-acetylgalactosaminyltransferase 9
    EC=2.4.1.41
Alternative name(s):
    Polypeptide GalNAc transferase 9
      Short name=pp-GaNTase 9
      Short name=GalNAc-T9
    Protein-UDP acetylgalactosaminyltransferase 9
    UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 9

Gene names

Name:	GALNT9
ORF Names:	QnpA-13140

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	606 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Does not glycosylate apomucin or SDC3 By similarity.
Catalytic activity	UDP-N-acetyl-D-galactosamine + polypeptide = UDP + N-acetyl-D-galactosaminyl-polypeptide.
Cofactor	Manganese By similarity. Calcium By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Domain	There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity. The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.
Sequence similarities	Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Calcium Lectin Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW polypeptide N-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 606	606	Polypeptide N-acetylgalactosaminyltransferase 9		PRO_0000059121
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 29	23	Signal-anchor for type II membrane
	Topological domain	30 – 606	577	Lumenal Potential
	Domain	467 – 603	137	Ricin B-type lectin
	Region	153 – 264	112	Catalytic subdomain A
	Region	321 – 383	63	Catalytic subdomain B
Amino acid modifications
	Glycosylation	463	1	N-linked (GlcNAc...) Potential
	Disulfide bond	480 ↔ 496		By similarity
	Disulfide bond	528 ↔ 543		By similarity
	Disulfide bond	570 ↔ 590		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9GM01-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: A00A97C02BD3BBBD	FASTA	606	68,318
	10 20 30 40 50 60 MAVARKIRTL LTVNILVFVG IVLFSVYCRL QGRSQELVRP LSGGCRPRPA TPAPGSPLRS 70 80 90 100 110 120 GGPRAVVAGR AELVLKGQLP AEPCSPGRLM VPEAEAEAQG GLAATLRDDG QEAEGKYEEY 130 140 150 160 170 180 GYNAQLSDRI SLDRSIPDYR PRKCRHMSYA QDLPQVSVVF IFVNEALSVI LRSVHSVVNH 190 200 210 220 230 240 TPSQLLKEVI LVDDNSDNVE LKFNLDQYVN KRYPGLVKIV RNSRREGLIR ARLQGWKAAT 250 260 270 280 290 300 APVVGFFDAH VEFNTGWAEP ALSRIREDRR RIVLPAIDNI KYSTFEVQQY ANAAHGYNWG 310 320 330 340 350 360 LWCMYIIPPQ DWLDRGDESA PIRTPAMIGC SFVVDREYFG DIGLLDPGME VYGGENVELG 370 380 390 400 410 420 MRVWQCGGSM EVLPCSRVAH IERTRKPYNN DIDYYAKRNA LRAAEVWMDD FKSHVYMAWN 430 440 450 460 470 480 IPMTNPGVDF GDVSERLALR QRLKCRSFKW YLENVYPEMR IYNNTLTYGE VRNSKASGYC 490 500 510 520 530 540 LDQGAEDGDR AILYPCHGMS SQLVRYSADG LLQLGPLGST AFLPDSKCLV DDGRGRTPTL 550 560 570 580 590 600 RKCEDVARPT QRLWDFTQSG PIVSRATGRC LEVEMSKDAN FGLRLVVQRC SGQKWMIRNW IKHARH

References

[1]

"Isolation of full-length cDNA clones from macaque brain cDNA libraries." Osada N., Hida M., Kusuda J., Tanuma R., Iseki K., Hirai M., Terao K., Suzuki Y., Sugano S., Hashimoto K. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Parietal cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB050509 mRNA. Translation: BAB17277.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	CBM13. Carbohydrate-Binding Module Family 13. GT27. Glycosyltransferase Family 27.
Phylogenomic databases
HOVERGEN	Q9GM01.
Enzyme and pathway databases
BRENDA	2.4.1.41. 3438.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. IPR000772. Ricin_B_lectin. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. PF00652. Ricin_B_lectin. 1 hit. [Graphical view]
SMART	SM00458. RICIN. 1 hit. [Graphical view]
PROSITE	PS50231. RICIN_B_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GALT9_MACFA

Accession

Primary (citable) accession number: Q9GM01

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	March 1, 2001
Last modified:	October 13, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)