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Protein

Polypeptide N-acetylgalactosaminyltransferase 9

Gene

GALNT9

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Does not glycosylate apomucin or SDC3.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.By similarity

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei194SubstrateBy similarity1
Binding sitei225SubstrateBy similarity1
Metal bindingi248ManganeseBy similarity1
Metal bindingi250ManganeseBy similarity1
Metal bindingi380ManganeseBy similarity1
Binding sitei383SubstrateBy similarity1
Binding sitei388SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandLectin, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 9 (EC:2.4.1.41By similarity)
Alternative name(s):
Polypeptide GalNAc transferase 9
Short name:
GalNAc-T9
Short name:
pp-GaNTase 9
Protein-UDP acetylgalactosaminyltransferase 9
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 9
Gene namesi
Name:GALNT9
ORF Names:QnpA-13140
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 29Helical; Signal-anchor for type II membrane proteinAdd BLAST23
Topological domaini30 – 606LumenalSequence analysisAdd BLAST577

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000591211 – 606Polypeptide N-acetylgalactosaminyltransferase 9Add BLAST606

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi144 ↔ 375PROSITE-ProRule annotation
Disulfide bondi366 ↔ 445PROSITE-ProRule annotation
Glycosylationi463N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi480 ↔ 496PROSITE-ProRule annotation
Disulfide bondi528 ↔ 543PROSITE-ProRule annotation
Disulfide bondi570 ↔ 590PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ9GM01.
SMRiQ9GM01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini467 – 603Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST137

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 264Catalytic subdomain AAdd BLAST112
Regioni321 – 383Catalytic subdomain BAdd BLAST63

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG051699.
KOiK00710.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiView protein in InterPro
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
IPR001202. WW_dom.
PfamiView protein in Pfam
PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
SMARTiView protein in SMART
SM00458. RICIN. 1 hit.
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiView protein in PROSITE
PS50231. RICIN_B_LECTIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9GM01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVARKIRTL LTVNILVFVG IVLFSVYCRL QGRSQELVRP LSGGCRPRPA
60 70 80 90 100
TPAPGSPLRS GGPRAVVAGR AELVLKGQLP AEPCSPGRLM VPEAEAEAQG
110 120 130 140 150
GLAATLRDDG QEAEGKYEEY GYNAQLSDRI SLDRSIPDYR PRKCRHMSYA
160 170 180 190 200
QDLPQVSVVF IFVNEALSVI LRSVHSVVNH TPSQLLKEVI LVDDNSDNVE
210 220 230 240 250
LKFNLDQYVN KRYPGLVKIV RNSRREGLIR ARLQGWKAAT APVVGFFDAH
260 270 280 290 300
VEFNTGWAEP ALSRIREDRR RIVLPAIDNI KYSTFEVQQY ANAAHGYNWG
310 320 330 340 350
LWCMYIIPPQ DWLDRGDESA PIRTPAMIGC SFVVDREYFG DIGLLDPGME
360 370 380 390 400
VYGGENVELG MRVWQCGGSM EVLPCSRVAH IERTRKPYNN DIDYYAKRNA
410 420 430 440 450
LRAAEVWMDD FKSHVYMAWN IPMTNPGVDF GDVSERLALR QRLKCRSFKW
460 470 480 490 500
YLENVYPEMR IYNNTLTYGE VRNSKASGYC LDQGAEDGDR AILYPCHGMS
510 520 530 540 550
SQLVRYSADG LLQLGPLGST AFLPDSKCLV DDGRGRTPTL RKCEDVARPT
560 570 580 590 600
QRLWDFTQSG PIVSRATGRC LEVEMSKDAN FGLRLVVQRC SGQKWMIRNW

IKHARH
Length:606
Mass (Da):68,318
Last modified:March 1, 2001 - v1
Checksum:iA00A97C02BD3BBBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050509 mRNA. Translation: BAB17277.1.
RefSeqiNP_001306356.1. NM_001319427.1.
UniGeneiMfa.1709.

Genome annotation databases

GeneIDi102117136.
KEGGimcf:102117136.

Similar proteinsi

Entry informationi

Entry nameiGALT9_MACFA
AccessioniPrimary (citable) accession number: Q9GM01
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: March 1, 2001
Last modified: September 27, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families