Reviewed, UniProtKB/Swiss-Prot Q9GLR1 (NEC1_BOVIN)

Names and origin

Protein names

Recommended name:
    Neuroendocrine convertase 1
      Short name=NEC 1
    EC=3.4.21.93
Alternative name(s):
    Prohormone convertase 1
    Proprotein convertase 1
      Short name=PC1

Gene names

Name:

PCSK1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	753 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin By similarity.
Catalytic activity	Release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.
Cofactor	Calcium By similarity.
Subcellular location	Cytoplasmic vesicle › secretory vesicle By similarity. Note: Localized in the secretion granules By similarity.
Sequence similarities	Belongs to the peptidase S8 family. Furin subfamily.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle
Domain	Signal
Ligand	Calcium
Molecular function	Hydrolase Protease Serine protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	secretory granule Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Propeptide	28 – 110	83	Potential		PRO_0000244606
	Chain	111 – 753	643	Neuroendocrine convertase 1		PRO_0000244607
Regions
	Region	122 – 410	289	Catalytic
	Region	739 – 751	13	Amphipathic Potential
Sites
	Active site	167	1	Charge relay system By similarity
	Active site	208	1	Charge relay system By similarity
	Active site	382	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	173	1	N-linked (GlcNAc...) Potential
	Glycosylation	401	1	N-linked (GlcNAc...) Potential
	Disulfide bond	225 ↔ 374		By similarity
	Disulfide bond	317 ↔ 347		By similarity
	Disulfide bond	467 ↔ 494		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9GLR1-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 577AFEACB47B0A59	FASTA	753	83,807
	10 20 30 40 50 60 MGRRAWTLQC TAFSLFCAWC AMNSVKAKKQ FVNEWAAEIP GGPEAASAIA QELGYDLLGQ 70 80 90 100 110 120 IGSLENHYLF KHRNHPRRSR RSALHITKRL SDDDRVIWAE QQYEKERSKR SVLRDSALDL 130 140 150 160 170 180 FNDPMWNQQW YLQDTRMTAT LPKLDLHVIP VWQKGITGKG VVITVLDDGL EWNHTDIYAN 190 200 210 220 230 240 YDPEASYDFN DNDHDPFPRY DLINENKHGT RCAGEIAMQA NNHKCGVGVA YNSKVGGIRM 250 260 270 280 290 300 LDGIVTDAIE ASLIGFNPGH VDIYSASWGP NDDGKTVEGP GRLAQKAFEY GVKQGRQGKG 310 320 330 340 350 360 SIFVWASGNG GRQGDNCDCD GYTDSIYTIS INSASQQGLS PWYAEKCSST LATSYSSGDY 370 380 390 400 410 420 TDQRITSADL HNDCTETHTG TSASAPLAAG IFALALEANP NLTWRDMQHL VVWTSEYDPL 430 440 450 460 470 480 ANNPGWKKNG AGLMVNSRFG FGLLNAKALV DLADPSTWSS VPEKKECVVK DNDFEPRALK 490 500 510 520 530 540 ANGEVIIEIP TRACEGQENA IKSLEHVQFE ATIEYSRRGD LHVTLTSAAG TSTVLLAERE 550 560 570 580 590 600 RDTSPNGFKN WDFMSVHTWG ENPIGTWTLR IADMSGRIQN EGRIVTWKLI LHGTSSQPEH 610 620 630 640 650 660 MKQPRVYTSY NTVQNDRRGV EKVVDSGEEQ PTQEGLDENA QASQSPSGSG VGGRRDELAE 670 680 690 700 710 720 GAPSEAMLRL LQSAFSKNSP SKQSPKKPPS AKPNIPYENF YEALERLNKP SQLKDSEDSL 730 740 750 YNDYVDGFYN TKPYKHRDDR LLQALVDLLR EEN

References

[1]	"Molecular cloning demonstrates structural features of homologous bovine prohormone convertases 1 and 2." Hwang S.-R., Ng S.-M., Steineckert B., Seidah N.G., Hook V.Y.H. DNA Cell Biol. 19:409-419(2000) [PubMed: 10945231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal medulla.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF186405 mRNA. Translation: AAG17017.1.
IPI	IPI00692301.
RefSeq	NP_776837.1.
UniGene	Bt.5562
3D structure databases
HSSP	HSSP built from PDB template 1KN6 based on UniProtKB P21662.
ModBase	Search...
Protein family/group databases
MEROPS	S08.072.
Genome annotation databases
Ensembl	ENSBTAG00000020843. Bos taurus. [Contig view]
GeneID	281967.
KEGG	bta:281967.
Phylogenomic databases
HOVERGEN	Q9GLR1.
Enzyme and pathway databases
BRENDA	3.4.21.93. 251.
Family and domain databases
InterPro	IPR000209. Pept_S8_S53. IPR015500. Peptidase_S8_subtilisin-rel. IPR002884. PrprotnconvertsP. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF01483. P_proprotein. 1 hit. PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
ProDom	PD000717. PrprotnconvertsP. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00136. SUBTILASE_ASP. 1 hit. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NEC1_BOVIN

Accession

Primary (citable) accession number: Q9GLR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)