Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Neuroendocrine convertase 1

Gene

PCSK1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin (By similarity).By similarity

Catalytic activityi

Release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei167 – 1671Charge relay systemBy similarity
Active sitei208 – 2081Charge relay systemBy similarity
Active sitei382 – 3821Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium

Protein family/group databases

MEROPSiS08.072.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroendocrine convertase 1 (EC:3.4.21.93)
Short name:
NEC 1
Alternative name(s):
Prohormone convertase 1
Proprotein convertase 1
Short name:
PC1
Gene namesi
Name:PCSK1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Cytoplasmic vesiclesecretory vesicle By similarity
Note: Localized in the secretion granules.By similarity

GO - Cellular componenti

  1. extracellular space Source: GO_Central
  2. Golgi apparatus Source: GO_Central
  3. transport vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Propeptidei28 – 11083Sequence AnalysisPRO_0000244606Add
BLAST
Chaini111 – 753643Neuroendocrine convertase 1PRO_0000244607Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi225 ↔ 374By similarity
Disulfide bondi317 ↔ 347By similarity
Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi467 ↔ 494By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ9GLR1.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027775.

Structurei

3D structure databases

ProteinModelPortaliQ9GLR1.
SMRiQ9GLR1. Positions 31-103, 122-597.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini162 – 451290Peptidase S8Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S8 family. Furin subfamily.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4935.
HOGENOMiHOG000192536.
HOVERGENiHBG008705.
InParanoidiQ9GLR1.
KOiK01359.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR022005. Proho_convert.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PF12177. Proho_convert. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9GLR1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRRAWTLQC TAFSLFCAWC AMNSVKAKKQ FVNEWAAEIP GGPEAASAIA
60 70 80 90 100
QELGYDLLGQ IGSLENHYLF KHRNHPRRSR RSALHITKRL SDDDRVIWAE
110 120 130 140 150
QQYEKERSKR SVLRDSALDL FNDPMWNQQW YLQDTRMTAT LPKLDLHVIP
160 170 180 190 200
VWQKGITGKG VVITVLDDGL EWNHTDIYAN YDPEASYDFN DNDHDPFPRY
210 220 230 240 250
DLINENKHGT RCAGEIAMQA NNHKCGVGVA YNSKVGGIRM LDGIVTDAIE
260 270 280 290 300
ASLIGFNPGH VDIYSASWGP NDDGKTVEGP GRLAQKAFEY GVKQGRQGKG
310 320 330 340 350
SIFVWASGNG GRQGDNCDCD GYTDSIYTIS INSASQQGLS PWYAEKCSST
360 370 380 390 400
LATSYSSGDY TDQRITSADL HNDCTETHTG TSASAPLAAG IFALALEANP
410 420 430 440 450
NLTWRDMQHL VVWTSEYDPL ANNPGWKKNG AGLMVNSRFG FGLLNAKALV
460 470 480 490 500
DLADPSTWSS VPEKKECVVK DNDFEPRALK ANGEVIIEIP TRACEGQENA
510 520 530 540 550
IKSLEHVQFE ATIEYSRRGD LHVTLTSAAG TSTVLLAERE RDTSPNGFKN
560 570 580 590 600
WDFMSVHTWG ENPIGTWTLR IADMSGRIQN EGRIVTWKLI LHGTSSQPEH
610 620 630 640 650
MKQPRVYTSY NTVQNDRRGV EKVVDSGEEQ PTQEGLDENA QASQSPSGSG
660 670 680 690 700
VGGRRDELAE GAPSEAMLRL LQSAFSKNSP SKQSPKKPPS AKPNIPYENF
710 720 730 740 750
YEALERLNKP SQLKDSEDSL YNDYVDGFYN TKPYKHRDDR LLQALVDLLR

EEN
Length:753
Mass (Da):83,807
Last modified:March 1, 2001 - v1
Checksum:i577AFEACB47B0A59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF186405 mRNA. Translation: AAG17017.1.
RefSeqiNP_776837.1. NM_174412.2.
UniGeneiBt.5562.

Genome annotation databases

GeneIDi281967.
KEGGibta:281967.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF186405 mRNA. Translation: AAG17017.1.
RefSeqiNP_776837.1. NM_174412.2.
UniGeneiBt.5562.

3D structure databases

ProteinModelPortaliQ9GLR1.
SMRiQ9GLR1. Positions 31-103, 122-597.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027775.

Protein family/group databases

MEROPSiS08.072.

Proteomic databases

PRIDEiQ9GLR1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281967.
KEGGibta:281967.

Organism-specific databases

CTDi5122.

Phylogenomic databases

eggNOGiCOG4935.
HOGENOMiHOG000192536.
HOVERGENiHBG008705.
InParanoidiQ9GLR1.
KOiK01359.

Miscellaneous databases

NextBioi20805838.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR022005. Proho_convert.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PF12177. Proho_convert. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning demonstrates structural features of homologous bovine prohormone convertases 1 and 2."
    Hwang S.-R., Ng S.-M., Steineckert B., Seidah N.G., Hook V.Y.H.
    DNA Cell Biol. 19:409-419(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Adrenal medulla.

Entry informationi

Entry nameiNEC1_BOVIN
AccessioniPrimary (citable) accession number: Q9GLR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 1, 2001
Last modified: February 4, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.