Reviewed, UniProtKB/Swiss-Prot Q9GLR0 (NEC2_BOVIN)

Names and origin

Protein names

Recommended name:
    Neuroendocrine convertase 2
      Short name=NEC 2
    EC=3.4.21.94
Alternative name(s):
    Prohormone convertase 2
    Proprotein convertase 2
      Short name=PC2

Gene names

Name:

PCSK2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues By similarity.
Catalytic activity	Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.
Subcellular location	Cytoplasmic vesicle › secretory vesicle By similarity. Note: Localized in the secretion granules By similarity.
Sequence similarities	Belongs to the peptidase S8 family. Furin subfamily.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Cleavage on pair of basic residues Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	nervous system development Inferred from sequence or structural similarity. Source: AgBase protein autoprocessing Inferred from sequence or structural similarity. Source: AgBase proteolysis Inferred from sequence or structural similarity. Source: AgBase
Cellular component	secretory granule Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Propeptide	26 – 109	84	Potential		PRO_0000244608
	Chain	110 – 638	529	Neuroendocrine convertase 2		PRO_0000244609
Regions
	Region	122 – 415	294	Catalytic
Sites
	Active site	167	1	Charge relay system By similarity
	Active site	208	1	Charge relay system By similarity
	Active site	384	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	375	1	N-linked (GlcNAc...) Potential
	Glycosylation	514	1	N-linked (GlcNAc...) Potential
	Glycosylation	524	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9GLR0-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: DFF114AD9EC2AE68	FASTA	638	70,247
	10 20 30 40 50 60 MRGGCISQGK AAAGLLFCVM VFASAERPVF TNHFLVELHR GGEEKARQVA AEHGFGVRKL 70 80 90 100 110 120 PFAEGLYHFY HNGLAKARRR RSLQHQQQLE RDPRVKRALQ QEGFNRKKRG YRDINEIDIN 130 140 150 160 170 180 VNDPLFTKQW YLINTGQADG TPGLDLNVAE AWELGYTGKG VTIGIMDDGI DYLHPDLASN 190 200 210 220 230 240 YNAEASYDFS SNDPYPYPRY TDDWSNSHGT RCAGEVSAAA NNNICGVGVA YGSKVAGIRM 250 260 270 280 290 300 LDQPFMTDII EASSISHMPQ LIDIYSASWG PTDNGKTVDG PRELTLQAMA DGVNKGRGGK 310 320 330 340 350 360 GSIYVWASGD GGSYDDCNCD GYASSMWTIS INSAINDGRT ALYDESCSST LASTFSNGRK 370 380 390 400 410 420 RNPEAGVATT DLYGNCTLRH SGTSAAAPEA AGVFALALEA NLGLTWRDMQ HLTVLTSKRN 430 440 450 460 470 480 QLHDEVHQWR RNGVGLEFNH LFGYGVLDAG AMVKMAKDWK TVPERFHCVG GSVQNPEKIP 490 500 510 520 530 540 TTGKPVLTLT TDACEGKENF VRYLEHVQAV VTVNATRRGD LNINMTSPMG TKSILLSRRP 550 560 570 580 590 600 RGDDAKVGFD KWPFMTTHTW GEDARGTWIL ELGFVGSAPQ KGVLMEWTLM LHGTQSAPYI 610 620 630 DQVVRDYQSK LAMSKKEELE EELDEAVQRS LKSILGKD

References

[1]	"Molecular cloning demonstrates structural features of homologous bovine prohormone convertases 1 and 2." Hwang S.-R., Ng S.-M., Steineckert B., Seidah N.G., Hook V.Y.H. DNA Cell Biol. 19:409-419(2000) [PubMed: 10945231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal medulla.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF186406 mRNA. Translation: AAG17018.1.
IPI	IPI00685414.
RefSeq	NP_776838.1.
UniGene	Bt.5563
3D structure databases
SMR	Q9GLR0. Positions 30-101, 119-597.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9GLR0.
Protein family/group databases
MEROPS	S08.073.
Genome annotation databases
Ensembl	ENSBTAT00000011451; ENSBTAP00000011451; ENSBTAG00000008687; Bos taurus. [Genome view] ENSBTAT00000052049; ENSBTAP00000050841; ENSBTAG00000008687; Bos taurus. [Genome view]
GeneID	281968.
KEGG	bta:281968.
Organism-specific databases
CTD	281968.
Phylogenomic databases
eggNOG	maNOG04605.
HOVERGEN	Q9GLR0.
InParanoid	Q9GLR0.
PhylomeDB	Q9GLR0.
Enzyme and pathway databases
BRENDA	3.4.21.94. 251.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR000209. Peptidase_S8/S53. IPR015500. Peptidase_S8_subtilisin-rel. IPR009020. Prot_inh_propept. IPR002884. PrprotnconvertsP. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF01483. P_proprotein. 1 hit. PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
PROSITE	PS00136. SUBTILASE_ASP. 1 hit. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NEC2_BOVIN

Accession

Primary (citable) accession number: Q9GLR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)