Reviewed, UniProtKB/Swiss-Prot Q9GLK0 (TGM1_CANFA)

Names and origin

Protein names

Recommended name:
    Protein-glutamine gamma-glutamyltransferase K
    EC=2.3.2.13
Alternative name(s):
    Transglutaminase K
      Short name=TGase K
      Short name=TGK
      Short name=TG(K)
    Transglutaminase-1
    Epidermal TGase

Gene names

Name:

TGM1

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	815 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum By similarity.
Catalytic activity	Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the transglutaminase superfamily. Transglutaminase family.

Ontologies

Keywords
Biological process	Keratinization
Cellular component	Membrane
Ligand	Calcium Metal-binding
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	keratinization Inferred from electronic annotation. Source: UniProtKB-KW peptide cross-linking Inferred from electronic annotation. Source: InterPro
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein-glutamine gamma-glutamyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 815	815	Protein-glutamine gamma-glutamyltransferase K		PRO_0000213700
Sites
	Active site	375	1	By similarity
	Active site	434	1	By similarity
	Active site	457	1	By similarity
	Metal binding	497	1	Calcium By similarity
	Metal binding	499	1	Calcium By similarity
	Metal binding	546	1	Calcium By similarity
	Metal binding	551	1	Calcium By similarity
Amino acid modifications
	Modified residue	90	1	Phosphoserine By similarity
	Modified residue	93	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9GLK0-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 82C49D15DDD25BE4	FASTA	815	89,757
	10 20 30 40 50 60 MDGPRSDVGR WGGNPWQPPT TPSPEPEPEP EPERRSRRGG RSFWARCCGC CSCRNRADDD 70 80 90 100 110 120 WGPEPHRDRG SGSGRRRPDS RGSDSRRPGS RASGVNAAGD GTIREGMLVV TGVDLLSSRS 130 140 150 160 170 180 DQNRREHHTD EFEYDELIIR RGQPFHMVLH FSRPYESSDR VALELLIGNN PEVGKGTHVI 190 200 210 220 230 240 IPVGKGGSGG WKAQVTKASG QNLNLRVHTS PNAIIGKFQF TVRTHSEAGE FQLPFDPHNE 250 260 270 280 290 300 IYILFNPWCP EDIVYVDHED WRQEYVLNES GRIYYGTEAQ IGERTWNYGQ FDHGVLDACL 310 320 330 340 350 360 YILDRRGMPY GGRGDPVSVS RVISAMVNSL DDNGVLIGNW SGDYSRGTNP SAWVGSVEIL 370 380 390 400 410 420 LSYLRTGYSV PYGQCWVFAG VTTTVLRCLG LATRTVTNFN SAHDTDTSLT MDIYFDENMK 430 440 450 460 470 480 PLEHLNHDSV WNFHVWNDCW MKRPDLPSGF DGWQVVDATP QETSSGIFCC GPCSVESIKN 490 500 510 520 530 540 GLVYMKYDTP FIFAEVNSDK VYWQRQDDGS FKIVYVEEKA IGTLIVTKAV GSNMQDDVTH 550 560 570 580 590 600 IYKHPEGSEA ERKAVETAAA HGSKPNVYTN RDSAEDVALQ VEAQDAVMGQ DLTVSVVLTN 610 620 630 640 650 660 RGSSTRTVKL HLYLSVTFYT GVTGPVFKES KKEVVLAPGA TERVSMPVAY KEYRPQIVDQ 670 680 690 700 710 720 GSMLLNVSGH VKENGQVLAK QHTFRLRTPD LSLTLLGAAV VGQECEVQIV FKNPLPVTLT 730 740 750 760 770 780 NVVFRLEGSG LQRPKILNVG DIGGNETVTL HQKFVPVRPG PRQLIASLDS PQLSQVHGVI 790 800 810 QVDVAPAPGG GGFFSNAGGN SPLGETIPMA SRGGA

References

[1]	"DNA sequence and physical mapping of the canine transglutaminase 1 gene." Credille K.M., Venta P.J., Breen M., Lowe J.K., Murphy K.E., Ostrander E.A., Galibert F., Dunstan R.W. Cytogenet. Cell Genet. 93:73-76(2001) [PubMed: 11474183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF262219 mRNA. Translation: AAG13662.1.
RefSeq	NP_001003079.1.
UniGene	Cfa.3494
3D structure databases
HSSP	HSSP built from PDB template 1EX0 based on UniProtKB P00488.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCAFG00000012182. Canis familiaris. [Contig view]
GeneID	403630.
KEGG	cfa:403630.
Phylogenomic databases
HOVERGEN	Q9GLK0.
Enzyme and pathway databases
BRENDA	2.3.2.13. 463.
Family and domain databases
InterPro	IPR008957. Fibronectin_typ-III-like_fold. IPR013783. Ig-like_fold. IPR002931. Transglutaminase-like. IPR008958. Transglutaminase_C. IPR013808. Transglutaminase_CS. IPR001102. Transglutaminase_N. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00927. Transglut_C. 2 hits. PF01841. Transglut_core. 1 hit. PF00868. Transglut_N. 1 hit. [Graphical view]
SMART	SM00460. TGc. 1 hit. [Graphical view]
PROSITE	PS00547. TRANSGLUTAMINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TGM1_CANFA

Accession

Primary (citable) accession number: Q9GLK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)