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Protein

Calpain-3

Gene

CAPN3

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease.

Catalytic activityi

Broad endopeptidase activity.

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei129PROSITE-ProRule annotation1
Active sitei334PROSITE-ProRule annotation1
Active sitei358PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi656 – 6661By similarityAdd BLAST11
Calcium bindingi699 – 7102PROSITE-ProRule annotationBy similarityAdd BLAST12
Calcium bindingi729 – 7353PROSITE-ProRule annotationBy similarity7
Calcium bindingi794 – 8004By similarity7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.54. 1793.

Protein family/group databases

MEROPSiC02.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-3 (EC:3.4.22.54)
Alternative name(s):
Calcium-activated neutral proteinase 3
Short name:
CANP 3
Calpain L3
Calpain p94
Cn94
Muscle-specific calcium-activated neutral protease 3
Gene namesi
Name:CAPN3
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002077071 – 815Calpain-3Add BLAST815

Proteomic databases

PRIDEiQ9GLG7.

Interactioni

Subunit structurei

Homodimer; via EF-hand domain 4. Interacts with TTN/titin. Interacts with CMYA5; this interaction, which results in CMYA5 proteolysis, may protect CAPN3 from autolysis.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ9GLG7.
SMRiQ9GLG7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 417Calpain catalyticPROSITE-ProRule annotationAdd BLAST344
Domaini643 – 677EF-hand 1PROSITE-ProRule annotationAdd BLAST35
Domaini686 – 719EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini716 – 751EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini781 – 815EF-hand 4PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni418 – 586Domain IIIAdd BLAST169
Regioni587 – 649LinkerAdd BLAST63
Regioni650 – 815Domain IVAdd BLAST166

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG012645.
KOiK08573.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
InterProiView protein in InterPro
IPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
PfamiView protein in Pfam
PF01067. Calpain_III. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
PRINTSiPR00704. CALPAIN.
SMARTiView protein in SMART
SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiView protein in PROSITE
PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9GLG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTVISASVA PRTAAEPRSP GPVPHPAQSK ATEAGGGNAS GIYSAIISRN
60 70 80 90 100
FPIIGVKEKT FEQLHKKCLE KKVLYVDPEF PPDETSLFYS QKFPIQFIWK
110 120 130 140 150
RPPEICENPR FIIDGANRTD ICQGDLGDCW FLAAIACLTL NQRLLFRVIP
160 170 180 190 200
HDQSFIENYA GIFHFQFWRY GEWVDVVIDD CLPTYNNQLV FTKSNHRNEF
210 220 230 240 250
WSALLEKAYA KLHGSYEALK GGNTTEAMED FTGGVTEFFE IRDAPSDMHK
260 270 280 290 300
IMKKAIERGS LMGCSIDDGT NMTYGTSPSG LNMGELIARM VRNMDNSLFR
310 320 330 340 350
DSDLDPRASV ERPTRTIVPV QYETRMACGL VRGHAYSVTG LDEVLFKGEK
360 370 380 390 400
VKLVRLRNPW GQVEWNGSWS DGWKDWSFVD KDEKARLQHQ VTEDGEFWMS
410 420 430 440 450
YEDFIYHFTK LEICNLTADA LQSDKLQTWT VSVNEGRWVR GCSAGGCRNF
460 470 480 490 500
PDTFWTNPQY RLKLLEEDDD PDDSEVICSF LVALMQKNRR KDRKLGANLF
510 520 530 540 550
TIGFAIYEVP KEMHGNRQHL QKDFFLYNAS RARSKTYINM REVSQRFRLP
560 570 580 590 600
PSEYVIVPST YEPHQEGEFI LRVFSEKRNL SEEVENTISV DRPVPIIFVS
610 620 630 640 650
DRANSNKELG VDQESEEGKG KTSPDKQEQS PQPQPGSSDQ ESEEQQQFRN
660 670 680 690 700
IFKQIAGDDM EICADELKKV LNTVVNKHKD LKTHGFTLES CRSMIALMDT
710 720 730 740 750
DGSGKLNLQE FHHLWNKIKA WQKIFKHYDT DQSGTINSYE MRNAVNDAGF
760 770 780 790 800
HLNNQLYDII TMRYADKHMN IDFDSFICCF VRLEGMFRAF HAFDKDGDGI
810
IKLNVLEWLQ LTMYA
Length:815
Mass (Da):93,556
Last modified:March 1, 2001 - v1
Checksum:i994A930F0223CCF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF277376 mRNA. Translation: AAG27599.1.
RefSeqiNP_001274630.1. NM_001287701.1.
UniGeneiMfa.5713.

Genome annotation databases

GeneIDi102115794.
KEGGimcf:102115794.

Similar proteinsi

Entry informationi

Entry nameiCAN3_MACFA
AccessioniPrimary (citable) accession number: Q9GLG7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: March 1, 2001
Last modified: August 30, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families