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Q9GLG2

- CAN1_MACFA

UniProt

Q9GLG2 - CAN1_MACFA

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Protein
Calpain-1 catalytic subunit
Gene
CAPN1, CANPL1, QccE-12457
Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction By similarity.

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Binds 4 calcium ions By similarity.

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei15 – 162Cleavage; for 78 kDa form By similarity
Sitei27 – 282Cleavage; for 75 kDa form By similarity
Active sitei115 – 1151 By similarity
Active sitei272 – 2721 By similarity
Active sitei296 – 2961 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi99 – 10681 By similarity
Calcium bindingi302 – 333322 By similarity
Add
BLAST
Calcium bindingi598 – 609123 By similarity
Add
BLAST
Calcium bindingi628 – 639124 By similarity
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  1. proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.52. 3121.

Protein family/group databases

MEROPSiC02.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-1 catalytic subunit (EC:3.4.22.52)
Alternative name(s):
Calcium-activated neutral proteinase 1
Short name:
CANP 1
Calpain mu-type
Calpain-1 large subunit
Micromolar-calpain
Short name:
muCANP
Gene namesi
Name:CAPN1
Synonyms:CANPL1
ORF Names:QccE-12457
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 714713Calpain-1 catalytic subunit
PRO_0000207695Add
BLAST

Post-translational modificationi

Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms By similarity.

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PRIDEiQ9GLG2.

Expressioni

Tissue specificityi

Ubiquitous.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Structurei

3D structure databases

ProteinModelPortaliQ9GLG2.
SMRiQ9GLG2. Positions 13-713.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 354300Calpain catalytic
Add
BLAST
Domaini541 – 57636EF-hand 1
Add
BLAST
Domaini585 – 61834EF-hand 2
Add
BLAST
Domaini615 – 65036EF-hand 3
Add
BLAST
Domaini680 – 71435EF-hand 4
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni355 – 526172Domain III
Add
BLAST
Regioni527 – 54216Linker
Add
BLAST
Regioni543 – 713171Domain IV
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.
Contains 4 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG012645.
KOiK01367.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF13405. EF-hand_6. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9GLG2-1 [UniParc]FASTAAdd to Basket

« Hide

MAEEIITPVY CTGVSAQVQK QRAKELGLGR HENAIKYLGQ DYEQLRARCL    50
QSGTLFRDEA FPPVPQSLGF KDLGPNSSKT YGIKWKRPTE LLSNPQFIVD 100
GATRTDICQG ALGDCWLLAA IASLTLNDTL LHRVVPHGQS FQNGYAGIFH 150
FQLWQFGEWV DVVVDDLLPI KDGKLVFVHS AEGNEFWSAL LEKAYAKVNG 200
SYESLSGGST SEGFEDFTGG VTEWYELRKA PSDLYQIILK ALERGSLLGC 250
SIDISSVLDM EAITFKKLVK GHAYSVTGAK QVNYRGQMVN LIRMRNPWGE 300
VEWTGAWSDS SSEWNSVDPY EREQLRVKME DGEFWMSFRD FMREFTRLEI 350
CNLTPDALKS RTIRKWNTTL YEGTWRRGST AGGCRNYPAT FWVNPQFKIR 400
LDETDDPDDY GDRESGCSFV LALMQKHRRR ERRFGRDMET IGFAVYEVPP 450
ELVGQPALHL KRDFFLANAS RARSEQFINL REVSTRFRLP PGEYVVVPST 500
FEPNKEGDFV LRFFSEKSAG TAELDDQIQA NLPDEQVLSE EEIDENFKAL 550
FRQLAGEDME ISVKELRTIL NRIISKHKDL RTKGFSLESC RSMVNLMDRD 600
GNGKLGLVEF NILWNRIRNY LSIFRKFDLD KSGSMSAYEM RMAIESAGFK 650
LNKKLYELII TRYSEPDLAV DFDNFVCCLV RLETMFRFFK TLDTDLDGVV 700
TFDLFKWLQL TMFA 714
Length:714
Mass (Da):81,850
Last modified:March 1, 2001 - v1
Checksum:i834690C214DE7AD8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF284440 mRNA. Translation: AAG22770.1.
AB169651 mRNA. Translation: BAE01732.1.
RefSeqiNP_001272270.1. NM_001285341.1.
XP_005577364.1. XM_005577307.1.
XP_005577365.1. XM_005577308.1.
UniGeneiMfa.867.

Genome annotation databases

GeneIDi101867512.
KEGGimcf:101867512.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF284440 mRNA. Translation: AAG22770.1 .
AB169651 mRNA. Translation: BAE01732.1 .
RefSeqi NP_001272270.1. NM_001285341.1.
XP_005577364.1. XM_005577307.1.
XP_005577365.1. XM_005577308.1.
UniGenei Mfa.867.

3D structure databases

ProteinModelPortali Q9GLG2.
SMRi Q9GLG2. Positions 13-713.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C02.001.

Proteomic databases

PRIDEi Q9GLG2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 101867512.
KEGGi mcf:101867512.

Phylogenomic databases

HOVERGENi HBG012645.
KOi K01367.

Enzyme and pathway databases

BRENDAi 3.4.22.52. 3121.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
Pfami PF01067. Calpain_III. 1 hit.
PF13405. EF-hand_6. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Different expression patterns for ubiquitous calpains and Capn3 splice variants in monkey ocular tissues."
    Nakajima T., Fukiage C., Azuma M., Ma H., Shearer T.R.
    Biochim. Biophys. Acta 1519:55-64(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.
  2. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
    International consortium for macaque cDNA sequencing and analysis
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain cortex.

Entry informationi

Entry nameiCAN1_MACFA
AccessioniPrimary (citable) accession number: Q9GLG2
Secondary accession number(s): Q4R593
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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