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Q9GLG2

- CAN1_MACFA

UniProt

Q9GLG2 - CAN1_MACFA

Protein

Calpain-1 catalytic subunit

Gene

CAPN1

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.By similarity

    Catalytic activityi

    Broad endopeptidase specificity.

    Cofactori

    Binds 4 calcium ions.By similarity

    Enzyme regulationi

    Activated by micromolar concentrations of calcium and inhibited by calpastatin.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei15 – 162Cleavage; for 78 kDa formBy similarity
    Sitei27 – 282Cleavage; for 75 kDa formBy similarity
    Active sitei115 – 1151By similarity
    Active sitei272 – 2721By similarity
    Active sitei296 – 2961By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi99 – 10681PROSITE-ProRule annotation
    Calcium bindingi302 – 333322PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi598 – 609123PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi628 – 639124PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. proteolysis Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.4.22.52. 3121.

    Protein family/group databases

    MEROPSiC02.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calpain-1 catalytic subunit (EC:3.4.22.52)
    Alternative name(s):
    Calcium-activated neutral proteinase 1
    Short name:
    CANP 1
    Calpain mu-type
    Calpain-1 large subunit
    Micromolar-calpain
    Short name:
    muCANP
    Gene namesi
    Name:CAPN1
    Synonyms:CANPL1
    ORF Names:QccE-12457
    OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
    Taxonomic identifieri9541 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity
    Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 714713Calpain-1 catalytic subunitPRO_0000207695Add
    BLAST

    Post-translational modificationi

    Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms By similarity.By similarity

    Keywords - PTMi

    Autocatalytic cleavage

    Proteomic databases

    PRIDEiQ9GLG2.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Interactioni

    Subunit structurei

    Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

    Structurei

    3D structure databases

    ProteinModelPortaliQ9GLG2.
    SMRiQ9GLG2. Positions 13-713.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini55 – 354300Calpain catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini541 – 57636EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini585 – 61834EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini615 – 65036EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini680 – 71435EF-hand 4PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni355 – 526172Domain IIIAdd
    BLAST
    Regioni527 – 54216LinkerAdd
    BLAST
    Regioni543 – 713171Domain IVAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C2 family.Curated
    Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
    Contains 4 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOVERGENiHBG012645.
    KOiK01367.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view]
    PfamiPF01067. Calpain_III. 1 hit.
    PF13405. EF-hand_6. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view]
    PRINTSiPR00704. CALPAIN.
    SMARTiSM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 3 hits.
    [Graphical view]
    SUPFAMiSSF49758. SSF49758. 1 hit.
    PROSITEiPS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 4 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9GLG2-1 [UniParc]FASTAAdd to Basket

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    MAEEIITPVY CTGVSAQVQK QRAKELGLGR HENAIKYLGQ DYEQLRARCL    50
    QSGTLFRDEA FPPVPQSLGF KDLGPNSSKT YGIKWKRPTE LLSNPQFIVD 100
    GATRTDICQG ALGDCWLLAA IASLTLNDTL LHRVVPHGQS FQNGYAGIFH 150
    FQLWQFGEWV DVVVDDLLPI KDGKLVFVHS AEGNEFWSAL LEKAYAKVNG 200
    SYESLSGGST SEGFEDFTGG VTEWYELRKA PSDLYQIILK ALERGSLLGC 250
    SIDISSVLDM EAITFKKLVK GHAYSVTGAK QVNYRGQMVN LIRMRNPWGE 300
    VEWTGAWSDS SSEWNSVDPY EREQLRVKME DGEFWMSFRD FMREFTRLEI 350
    CNLTPDALKS RTIRKWNTTL YEGTWRRGST AGGCRNYPAT FWVNPQFKIR 400
    LDETDDPDDY GDRESGCSFV LALMQKHRRR ERRFGRDMET IGFAVYEVPP 450
    ELVGQPALHL KRDFFLANAS RARSEQFINL REVSTRFRLP PGEYVVVPST 500
    FEPNKEGDFV LRFFSEKSAG TAELDDQIQA NLPDEQVLSE EEIDENFKAL 550
    FRQLAGEDME ISVKELRTIL NRIISKHKDL RTKGFSLESC RSMVNLMDRD 600
    GNGKLGLVEF NILWNRIRNY LSIFRKFDLD KSGSMSAYEM RMAIESAGFK 650
    LNKKLYELII TRYSEPDLAV DFDNFVCCLV RLETMFRFFK TLDTDLDGVV 700
    TFDLFKWLQL TMFA 714
    Length:714
    Mass (Da):81,850
    Last modified:March 1, 2001 - v1
    Checksum:i834690C214DE7AD8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF284440 mRNA. Translation: AAG22770.1.
    AB169651 mRNA. Translation: BAE01732.1.
    RefSeqiNP_001272270.1. NM_001285341.1.
    XP_005577364.1. XM_005577307.1.
    XP_005577365.1. XM_005577308.1.
    UniGeneiMfa.867.

    Genome annotation databases

    GeneIDi101867512.
    KEGGimcf:101867512.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF284440 mRNA. Translation: AAG22770.1 .
    AB169651 mRNA. Translation: BAE01732.1 .
    RefSeqi NP_001272270.1. NM_001285341.1.
    XP_005577364.1. XM_005577307.1.
    XP_005577365.1. XM_005577308.1.
    UniGenei Mfa.867.

    3D structure databases

    ProteinModelPortali Q9GLG2.
    SMRi Q9GLG2. Positions 13-713.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C02.001.

    Proteomic databases

    PRIDEi Q9GLG2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 101867512.
    KEGGi mcf:101867512.

    Phylogenomic databases

    HOVERGENi HBG012645.
    KOi K01367.

    Enzyme and pathway databases

    BRENDAi 3.4.22.52. 3121.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view ]
    Pfami PF01067. Calpain_III. 1 hit.
    PF13405. EF-hand_6. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view ]
    PRINTSi PR00704. CALPAIN.
    SMARTi SM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 3 hits.
    [Graphical view ]
    SUPFAMi SSF49758. SSF49758. 1 hit.
    PROSITEi PS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 4 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Different expression patterns for ubiquitous calpains and Capn3 splice variants in monkey ocular tissues."
      Nakajima T., Fukiage C., Azuma M., Ma H., Shearer T.R.
      Biochim. Biophys. Acta 1519:55-64(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Retina.
    2. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
      International consortium for macaque cDNA sequencing and analysis
      Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain cortex.

    Entry informationi

    Entry nameiCAN1_MACFA
    AccessioniPrimary (citable) accession number: Q9GLG2
    Secondary accession number(s): Q4R593
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2003
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3